scholarly journals Differences in the antigenic profile of bloodstream and cell culture derived trypomastigotes of Trypanosoma cruzi

1989 ◽  
Vol 31 (3) ◽  
pp. 146-150
Author(s):  
Ana M. M. Da Silva ◽  
Cláudia I. Brodskyn ◽  
Harumi A. Takehara ◽  
I. Mota
Keyword(s):  
Cell Culture ◽  
Trypanosoma Cruzi ◽  
Comparative Study ◽  
Infected Blood ◽  
Chagasic Patient ◽  
Physiological Differences ◽  
Antigenic Profile

A comparative study of the antigenic profile of bloodstream and cell culture derived trypomastigotes showed many differences in their components. Using mouse anti-T. cruzi antibodies the differences were located mostly in the 120 kDa band, whereas using chagasic patient sera the differences were located in the 85 and 52 kDa bands. These findings might explain known physiological differences between trypomatigotes obtained from cell culture and from infected blood. A brief report of this work has already been published9.

scholarly journals A simple method to purify biologically and antigenically preserved bloodstream trypomastigotes of Trypanosoma cruzi using Deae-cellulose columns

1983 ◽  
Vol 78 (3) ◽  
pp. 317-333 ◽  
Author(s):  
Maria Auxiliadora de Sousa
Keyword(s):  
Trypanosoma Cruzi ◽  
Deae Cellulose ◽  
Mouse Blood ◽  
Simple Method ◽  
Infected Blood ◽  
Normal Morphology ◽  
Mean Survival Time ◽  
Triatomine Bugs ◽  
Forms Processing ◽  
Polymorphism Pattern

A method to purify trypanosomastigotes of some strains of Trypanosoma cruzi (Y, CL, FL, F, "Berenice", "Colombiana" and "São Felipe") from mouse blood by using DEAE-cellulose columns was standardized. This procedure is a modification of the Lanham & Godfrey methods and differs in some aspects from others described to purify T. cruzi bloodstream trypomastigotes, mainly by avoidance of prior purifications of parasites. By this method, the broad trypomastigotes were mainly isolated, accounting for higher recoveries obtained with strains having higher percentages of these forms: processing of infected blood from irradiated mice could be advantageous by increasing the recovery of parasites (percentage and/or total number) and elution of more slender trypomastigotes. Trypomastigotes purified by this method presented normal morphology and motility, remained infective to triatomine bugs and mice, showing in the latter prepatent periods and courses parasitemia similar to those of control parasites, and also reproducing the polymorphism pattern of each strain. Their virulence and pathogenicity also remained considerably preserved, the latter property being evaluated by LD 50 tests, mortality rates and mean survival time of inoculated mice. Moreover, these parasites presented positive, clear and peripheral immunofluorescence reaction at titres similar to those of control organisms, thus suggesting important preservation of their surface antigens.

Comparative study on the replication of HCV1b genome between wild‐type and cell culture‐adaptive mutant in regard to sensitivities against anti‐HCV drugs

2020 ◽  
Vol 64 (4) ◽  
pp. 296-303
Author(s):  
Yohei Miyayama ◽  
Heini Lee ◽  
HoJoong Song ◽  
Hiromi Abe‐Chayama ◽  
Daiki Miki ◽  
...  
Keyword(s):  
Cell Culture ◽  
Comparative Study ◽  
Wild Type

Comparative study of the biological properties of Trypanosoma cruzi I genotypes in a murine experimental model

2015 ◽  
Vol 29 ◽  
pp. 110-117 ◽  
Author(s):  
Lissa Cruz ◽  
Angie Vivas ◽  
Marleny Montilla ◽  
Carolina Hernández ◽  
Carolina Flórez ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Comparative Study ◽  
Experimental Model ◽  
Biological Properties

DNA as molecular target of analogous palladium and platinum anti-Trypanosoma cruzi compounds: A comparative study

2011 ◽  
Vol 105 (12) ◽  
pp. 1704-1711 ◽  
Author(s):  
Marisol Vieites ◽  
Pablo Smircich ◽  
Mariana Pagano ◽  
Lucía Otero ◽  
Francielle Luane Fischer ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Comparative Study ◽  
Molecular Target
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