scholarly journals Host cell adhesion to Schistosoma mansoni larvae in the peritoneal cavity of naive mice: histological and scanning electron microscopic studies

Author(s):  
Alan Lane de Melo ◽  
Conceição Ribeiro da Silva Machado ◽  
Leógenes Horácio Pereira

Cercariae of Schistosoma mansoni inoculated into the peritoneal cavity of naive mice induced host cell adhesion to their surface, but after 90 minutes the number of adherent cells sharply decreased. The cell detachment is progressive and simultaneous to the cercaria-schistosomule transformation. The histological study showed mainly neutrophils in close contact with the larvae. Mononuclear cells and some eosinophils were occasionally seen surrounding the adherent neutrophils. The scanning electron microscopy showed cells displaying twisted microvilli and several microplicae contacting or spreading over the larval surface, and larvae completely surrounded by clusters of cells. These results suggest that the neutrophils recognize molecules on the cercarial surface which induce their spreading

1992 ◽  
Vol 34 (3) ◽  
pp. 205-209 ◽  
Author(s):  
Alan L. Melo ◽  
Leógenes H. Pereira ◽  
Conceição R. S. Machado

The peritoneal cavity of laboratory mice was used to study the phenomenon of host cell adhesion to different evolutive stages of the Schistosoma mansoni (cercaria, adult worm, developing and mature eggs, miracidium, young and mature daughter sporocysts). Material recovered from the peritoneal cavity 30 and 180 min after the inoculation of each evolutive form was examined with the help of a stereomicroscope. The free swimming larvae (cercaria and miracidium), and the evolutive forms producing such larvae (mature egg and mature daughter sporocyst) elicited the host cell adhesion phenomenon. In all forms but cercariae the adherent cells remained as so till 180 minutes after inoculation


1993 ◽  
Vol 35 (3) ◽  
pp. 291-293
Author(s):  
Alan Lane de Melo ◽  
Leógenes Horácio Pereira

The treatment of naive mice with high closes of oxamniquine, 1 hour before the intraperitoneal inoculation of Schistosoma mansoni cercariae, induces a delay in the transformation process resulting in a longer host cell adhesion.


1989 ◽  
Vol 51 (4) ◽  
pp. 775-791 ◽  
Author(s):  
Mitsuo ABE ◽  
Kazushige TAKEHANA ◽  
Kenji IWASA ◽  
Takeo HIRAGA

1979 ◽  
Vol 88 (6_suppl4) ◽  
pp. 2-14 ◽  
Author(s):  
Malcolm D. Graham ◽  
Rodney Perkins

The structure of the normal human stapes was studied with the scanning electron microscope. Specimens were obtained 48 hours after death from adult human temporal bones free from obvious inflammatory disease. The specimens were fixed, dissected, critical-point dried and coated with gold. In this scanning electron microscopic study an attempt has been made to systematically demonstrate the average scanning electron microscopic features of various areas of the normal human stapes. An emphasis has been placed upon demonstrating as clearly as possible the details previously unclear or unrecognized and duplication of many excellent earlier light and electron microscopic studies has not been attempted. The typical appearance of the stapes head, neck, arch, crura and footplate has been presented. It is apparent that there exists a high degree of structural specialization particularly in the stapes arch and footplate area.


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