scholarly journals Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report

1995 ◽  
Vol 37 (4) ◽  
pp. 357-359 ◽  
Author(s):  
Vanda Akico Ueda Fick de Souza ◽  
Laura Masami Sumita ◽  
Mary Eiko S. Otsubo ◽  
Kioko Takei ◽  
Cláudio Sérgio Pannuti
Keyword(s):  
Enzyme Immunoassay ◽  
Correlation Coefficient ◽  
Filter Paper ◽  
Preliminary Report ◽  
Enzyme Linked Immunosorbent Assay ◽  
Simple Method ◽  
Blood Samples ◽  
Commercial Test ◽  
Antigen Production ◽  
Commercial Kit

A simple method of rubella antigen production by treatment with sodium desoxycholate for use in enzyme immunoassay (IMT-ELISA) is presented. When this assay was compared with a commercial test (Enzygnost-Rubella, Behring), in the study of 108 sera and 118 filter paper blood samples, 96.9% (219/226) overall agreement and correlation coefficient of 0.90 between absorbances were observed. Seven samples showed discordant results, negative by the commercial kit and positive by our test. Four of those 7 samples were available, being 3 positive by HI.

Enzyme immunoassay of free thyroxin in dried blood samples on filter paper.

1985 ◽  
Vol 31 (5) ◽  
pp. 750-753 ◽  
Author(s):  
N Hata ◽  
K Miyai ◽  
M Ito ◽  
Y Endo ◽  
Y Iijimi ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Room Temperature ◽  
Normal Subjects ◽  
Blood Samples ◽  
Double Antibody ◽  
Coefficients Of Variation ◽  
The Mean ◽  
Measurable Range

Abstract We describe a double-antibody enzyme immunoassay for determination of free thyroxin (FT4) in dried blood samples on filter paper, with use of a T4-beta-D-galactosidase complex. The measurable range of FT4 concentration in two 3-mm blood discs, each of which contained about 2.7 microL of blood, was 1.9 to 93 ng/L, as determined by comparison with concentrations of FT4 in known serum standards. FT4 in blood samples dried on filter paper was stable for at least four weeks when kept dry at -20 degrees C, room temperature, or 37 degrees C. The mean coefficients of variation were 7.6% (within assay) and 6.4% (between assays). Results for FT4 by this method correlated well with those for serum determined by radioimmunoassay (r = 0.98). The proposed method can be used to differentiate persons with hyper- and hypothyroidism from normal subjects and those with abnormal concentrations of thyroxin-binding globulin. The procedure seems suited for screening studies.

scholarly journals Fluorophotometric enzyme immunoassay of thyroxine in dried blood samples on filter paper.

1982 ◽  
Vol 31 (2) ◽  
pp. E55-E61 ◽  
Author(s):  
Hidetoshi ARAKAWA ◽  
Masako MAEDA ◽  
Akio TSUJI ◽  
Sumikazu ISHII ◽  
Hiroshi NARUSE ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Blood Samples

scholarly journals The Evolution of the Enzyme Immunoassay/Enzyme-Linked Immunosorbent Assay

2021 ◽  
Vol 2 (3) ◽  
pp. 13-17
Author(s):  
Leonid Tarassishin
Keyword(s):  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
High Specificity ◽  
Short Review ◽  
Immunological Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Target Antigen ◽  
Simple Method ◽  
Antibody Complex ◽  
Low Sensitivity

50 years ago the Enzyme Immunoassay Enzyme-Linked Immunosorbent Assay, mostly known as ELISA was developed. This is a powerful but simple method that is very widely used in the diagnostic practice, as well as in biomedical research. During this time a number of ELISA modification were developed that significantly increased its properties, especially the senstivity, such as avidin-biotin assay, immuno-PCR, nano-ELISA and finally, the digital ELISA. This short review describes the principles of ELISA and the evolution from a conventional assay to the modern ultra-sensitive method. Most of the immunological methods have two components: antigen and antibody. The high specificity of their interaction gives a possibility to detect one of them if other one is included in the reaction as a specific partner. The simplest method for antigen detection in the presence of the antibody is immune diffusion (radial immune diffusion in that case), which practically the formation of precipitate of the “antigen-antibody” complex, when the target antigen diffuses from well into agarose containing the specific antibody. Unfortunately, this assay, as well as other traditional methods, like hemagglutination or complement fixation, have a low sensitivity and are unwieldy.

Enzyme immunoassay of thyroxin-binding globulin in dried blood samples on filter paper.

1983 ◽  
Vol 29 (7) ◽  
pp. 1437-1440 ◽  
Author(s):  
N Hata ◽  
M Ito ◽  
H Mizuta ◽  
O Nose ◽  
K Miyai
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Room Temperature ◽  
Neonatal Hypothyroidism ◽  
Blood Samples ◽  
Coefficients Of Variation ◽  
The Mean ◽  
Positive Results ◽  
Measurable Concentration

Abstract A double-antibody enzyme immunoassay was developed for determination of thyroxin-binding globulin in dried blood samples on filter paper. The measurable concentration range of thyroxin-binding globulin in two 3-mm blood discs was 3.3 to 52 mg/L equivalent of serum (i.e., equivalent to the concentrations in known serum standards). Thyroxin-binding globulin in dried blood samples on filter paper was stable for at least four weeks when kept dry at -20 degrees C, 4 degrees C, or room temperature. The mean coefficients of variation were 6.6% (within assay) and 5.9% (between assays). The concentrations of thyroxin-binding globulin in dried blood samples determined by this method correlated well with those in serum determined by radioimmunoassay (r = 0.95) and by enzyme immunoassay (r = 0.96). This method is applicable for detecting cases of thyroxin-binding globulin deficiency and avoids the false-positive results for neonatal hypothyroidism obtained by measuring thyroxin.

Two-site enzyme immunoassay for alpha-fetoprotein in dried-blood samples collected on filter paper.

1986 ◽  
Vol 32 (11) ◽  
pp. 2079-2082 ◽  
Author(s):  
D Tsao ◽  
K J Hsiao ◽  
J C Wu ◽  
C K Chou ◽  
S D Lee
Keyword(s):  
Hepatocellular Carcinoma ◽  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Blood Donors ◽  
Alpha Fetoprotein ◽  
Polystyrene Bead ◽  
Blood Samples ◽  
Nitrophenyl Phosphate ◽  
High Risk Populations ◽  
Blood Spot

Abstract This is a method for measuring alpha-fetoprotein (AFP) in eluates of dried-blood samples on filter paper by use of a simple, sensitive two-site enzyme immunoassay. The spot, 6 mm in diameter (equivalent to about 12 microL of whole blood), is incubated overnight with alkaline phosphatase conjugated to rabbit anti-AFP antibody in a tube containing a polystyrene bead coated with mouse monoclonal antibody to AFP. After the beads are washed the enzyme activities associated with them are determined colorimetrically, with p-nitrophenyl phosphate as substrate. The measurable range of AFP is from 9 to 900 micrograms per liter of plasma. AFP in the dried-blood spot as determined by this method correlated well with the AFP value for serum from the same blood sample as determined by radioimmunoassay (r = 0.957, p less than 0.001). Preliminary studies in which we used this method with 242 healthy blood donors and 60 patients with hepatocellular carcinoma indicate that it may be suitable for use in mass screening for hepatocellular carcinoma in high-risk populations.

Enzyme immunoassay of thyroxine in serum and dried blood samples on filter paper.

1980 ◽  
Vol 27 (3) ◽  
pp. 375-380 ◽  
Author(s):  
KIYOSHI MIYAI ◽  
KAICHIRO ISHIBASHI ◽  
MINORU KAWASHIMA
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Blood Samples
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