scholarly journals Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection

2010 ◽  
Vol 52 (3) ◽  
pp. 125-128 ◽  
Author(s):  
Joyce Matie Kinoshita da Silva ◽  
Cibele Aparecida Villares ◽  
Maria do Socorro Monteiro ◽  
Carlos Colaúto ◽  
Anibal Ferreira dos Santos ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Breath Test ◽  
False Negative ◽  
Lateral Flow ◽  
Antigen Test ◽  
Kappa Index ◽  
Stool Antigen Test ◽  
Infection Diagnosis ◽  
Stool Antigen ◽  
H Pylori

The aim of this study was to validate the rapid lateral flow Helicobacter pylori stool antigen test (One step H. pylori antigen test, ACON laboratories, San Diego, USA; Prime diagnostics, São Paulo), using 13C-Urea Breath Test as the gold standard for H. pylori infection diagnosis. A total of 98 consecutive patients, asymptomatic or dyspeptic, entered the study. Sixty-nine were women, with a mean age of 45.76 ± 14.59 years (14 to 79 years). In the H. pylori-positive group, the rapid stool antigen test detected H. pylori antigen in 44 of the 50 positive patients (sensitivity 88%; 95% CI: 75.7-95.5%), and six false-negative; and in the H. pylori-negative group 42 presented negative results (specificity 87.5%; 95% CI: 74.7-95.3%), and six false-positive, showing a substantial agreement (Kappa Index = 0.75; p < 0.0001; 95% CI: 0.6-0.9). Forty four of fifty patients that had positive stool antigen were H. pylori-positive, the PPV of the stool antigen test was 88% (95% CI: 75.7-95.5%), and 42 patients with negative stool antigen test were H. pylori-negative, the NPV of the stool antigen test was 87.5% (95% CI: 74.7-95.3%). We conclude that the lateral flow stool antigen test can be used as an alternative to breath test for H. pylori infection diagnosis especially in developing countries.

scholarly journals Helicobacter pylori Infection in Infants and Toddlers in South America: Concordance between [13C]Urea Breath Test and Monoclonal H. pylori Stool Antigen Test

2013 ◽  
Vol 51 (11) ◽  
pp. 3735-3740 ◽  
Author(s):  
Dulciene Maria Magalhães Queiroz ◽  
Mayuko Saito ◽  
Gifone Aguiar Rocha ◽  
Andreia Maria Camargos Rocha ◽  
Fabrício Freire Melo ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
South America ◽  
Breath Test ◽  
Urea Breath Test ◽  
Infants And Toddlers ◽  
Antigen Test ◽  
Stool Antigen Test ◽  
Stool Antigen ◽  
13C Urea Breath Test ◽  
H Pylori

scholarly journals Evaluation of [13C]Urea Breath Test and Helicobacter pylori Stool Antigen Test for Diagnosis of H. pylori Infection in Children from a Developing Country

2003 ◽  
Vol 41 (7) ◽  
pp. 3334-3335 ◽  
Author(s):  
L. d. C. C. Cardinali ◽  
G. A. Rocha ◽  
A. M. C. Rocha ◽  
S. B. de Moura ◽  
T. de Figueiredo Soares ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Developing Country ◽  
Breath Test ◽  
Urea Breath Test ◽  
Antigen Test ◽  
Stool Antigen Test ◽  
Stool Antigen ◽  
13C Urea Breath Test ◽  
H Pylori

scholarly journals Investigating the Efficacy of Triple Drug Therapy and Sequential Drug Therapy in the Eradication of Helicobacter Pylori with Respect to Antigen Stool test: A Pilot Study

2020 ◽  
Vol 18 (1) ◽  
pp. 74-83
Author(s):  
R. Shrestha ◽  
R. Poudel ◽  
S. Shakya ◽  
R.B. Gurung ◽  
R. Makaju ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Drug Therapy ◽  
Sequential Therapy ◽  
Drug Regimen ◽  
Epidemiological Studies ◽  
Response To Treatment ◽  
Antigen Test ◽  
Stool Antigen Test ◽  
Stool Antigen ◽  
H Pylori

Background Helicobacter pylori is one of the most prevalent infectious disease worldwide. The treatment regimens involve mainly two therapies: Standard Triple drug therapy and Sequential drug therapy. Several studies have shown that the sequential therapy has higher eradication rates of H. pylori than the standard triple drug therapy and since proper study on sequential drug therapy and standard triple drug therapy is still lacking in Nepal, this study is attempted to compare efficacy of Sequential Drug Therapy in the eradication of H. pylori in gastritis with respect to the Standard triple drug therapy. Objective To investigate the efficacy of Triple Drug Therapy and Sequential Drug Therapy in the eradication of Helicobacter pylori with respect to Antigen Stool test. Method This study was the prospective study conducted in 62 patients attending the Department of Gastroenterology, Dhulikhel Hospital, meeting the inclusion criteria who were confirmed as H. pylori positive by histopathology and stool antigen test. Patients were randomized into two groups. One group prescribed with Standard triple drug regimen and another group with Sequential drug regimen. Eradication of H. pylori infection was confirmed by repeating the stool antigen test at least five weeks after the completion of the regimen. Result Among the 62 participants included in this study, 54.5% of them were males. Among the study population, the eradication achieved by standard triple drug therapy was 87.8% and 89.6% with Sequential drug therapy. Higher numbers (82.3%) of patients were compliant to the prescribed medication. Forgetfulness was the main reason for missing the dose (91%) of the non-compliant patients. Conclusion The study revealed an equal efficacy of both Standard Triple drug regimen and Sequential drug regimen in the eradication of H. pylori infection. Further, Stool antigen test can be preferred as a non-invasive test, for diagnosis of H. pylori infection, monitoring the response to treatment and in epidemiological studies.

scholarly journals The Use of 13C-Urea Breath Test for Non-Invasive Diagnosis of Helicobacter pylori Infection in Comparison to Endoscopy and Stool Antigen Test

Diagnostics ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 448
Author(s):  
Hamed Alzoubi ◽  
Asma’a Al-Mnayyis ◽  
Ibrahim Al rfoa ◽  
Amin Aqel ◽  
Mohammad Abu-Lubad ◽  
...  
Keyword(s):  
Breath Test ◽  
Eradication Therapy ◽  
Urea Breath Test ◽  
P Value ◽  
Antigen Test ◽  
Stool Antigen Test ◽  
Non Invasive ◽  
Stool Antigen ◽  
13C Urea Breath Test ◽  
H Pylori

Helicobacter pylori (H. pylori) can cause gastritis, peptic ulcer diseases and gastric carcinoma. Endoscopy as the gold standard method of diagnosis is an invasive procedure that might not be suitable in all scenarios. Therefore, this first study in Jordan aimed to assess the non-invasive 13C urea breath test (UBT) and stool antigen test for diagnosis of H. pylori infection and the successfulness of eradication therapy as alternatives for endoscopy. Hence, a total of 30 patients attending the endoscopy units at Alkarak teaching hospital were asked to complete a questionnaire with demographic and clinical data. They were then tested for H. pylori using 13C UBT and H. pylori stool antigen before having endoscopy. Another 30 patients who were positive for H. pylori by endoscopy were tested using both tests 6 weeks post eradication therapy. Results showed that the rate of H. pylori detection using endoscopy was 56.7% (17/30). Heartburns (82.3%, p value = 0.019), epigastric pain (88.2%, p value = 0.007) and vomiting (70.5%, p value = 0.02) were the most significant symptoms. Family history of peptic ulcer diseases was significantly associated with an increased risk for having a H. pylori positive result (p value = 0.02). Compared to endoscopy, the sensitivity of 13C UBT for the diagnosis of H. pylori was 94.1% (16/17), while it was 76.5% (13/17) for the stool antigen test. The specificity of both tests was equal (76.9%). However, the positive predictive and negative predictive values (84.2% and 90.9%) for 13C UBT were higher than those (81.3% and 71.4%) for the stool antigen test. The accuracy of 13C UBT was 86.7% compared to 76.7% for the stool antigen test. There was an 87% agreement (20 patients out of 23) between both tests when used to assess success of the eradication therapy. In conclusion, the 13C UBT was found to be more sensitive and accurate than the stool antigen test when used for diagnosis; furthermore, it has a comparable outcome to the stool antigen test in assessing the successfulness of the eradication treatment.

Comparison between the 13C-urea breath test and stool antigen test for the diagnosis of childhood Helicobacter pylori infection

2004 ◽  
Vol 39 (11) ◽  
pp. 1045-1050 ◽  
Author(s):  
Seiichi Kato ◽  
Keiko Nakayama ◽  
Takanori Minoura ◽  
Mutsuko Konno ◽  
Hitoshi Tajiri ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Breath Test ◽  
Urea Breath Test ◽  
Helicobacter Pylori Infection ◽  
Antigen Test ◽  
Stool Antigen Test ◽  
Stool Antigen ◽  
13C Urea Breath Test

scholarly journals Evaluation of a stool antigen test using a mAb for native catalase for diagnosis of Helicobacter pylori infection in children and adults

2014 ◽  
Vol 63 (12) ◽  
pp. 1621-1625 ◽  
Author(s):  
Masumi Okuda ◽  
Takako Osaki ◽  
Shogo Kikuchi ◽  
Junko Ueda ◽  
Yingsong Lin ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Real Time ◽  
Real Time Pcr ◽  
High Specificity ◽  
Antigen Test ◽  
Stool Antigen Test ◽  
Non Invasive ◽  
Significant Difference ◽  
Stool Antigen ◽  
H Pylori

Non-invasive diagnosis of Helicobacter pylori infection is important not only for screening of infection but also for epidemiological studies. Stool antigen tests are non-invasive and are convenient to identify H. pylori infection, particularly in children. We evaluated the stool antigen test, which uses a mAb for native catalase of H. pylori developed in Japan. A total of 151 stool samples were collected from participants (52 children and 99 adults) of the Sasayama Cohort Study and stored between −30 and −80 °C. The stool antigen test used was Testmate pylori antigen (TPAg), and was performed according to the manufacturer’s instructions. Furthermore, we conducted a quantitative real-time PCR test and compared the PCR results with those of the TPAg test. When compared with the results in real-time PCR, the sensitivity of TPAg was 89.5 % overall, 82.7 % for children and 92.4 % for adults, and the specificity was 100 %. The accuracy was 93.4 % overall, 90.4 % for children and 94.9 % for adults, and there was no significant difference in the accuracy of TPAg between children and adults. Five of 28 children (18 %) and five of 38 adults (13 %) were PCR positive with negative TPAg results. Four of five children with positive PCR and negative TPAg results were given a 13C-urea breath test and all four children tested negative. No significant correlation was observed between the TPAg results and DNA numbers of H. pylori in faeces among children or adults. A stool antigen test (TPAg) using a mAb for native catalase is useful for diagnosis of H. pylori in children and adults. Additionally, this test has particularly high specificity.

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