SEROLOGICAL AND MOLECULAR SURVEY OF Leptospira spp. AMONG CART HORSES FROM AN ENDEMIC AREA OF HUMAN LEPTOSPIROSIS IN CURITIBA, SOUTHERN BRAZIL

Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.

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Application of a real time Polymerase Chain Reaction (PCR) assay for the early diagnosis of human leptospirosis in Sri Lanka

Biologicals ◽

10.1016/j.biologicals.2016.09.004 ◽

2016 ◽

Vol 44 (6) ◽

pp. 497-502 ◽

Cited By ~ 6

Author(s):

D.T.H. Denipitiya ◽

N.V. Chandrasekharan ◽

W. Abeyewickreme ◽

C.M. Hartskeerl ◽

R.A. Hartskeerl ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Sri Lanka ◽

Early Diagnosis ◽

Real Time ◽

Chain Reaction ◽

Pcr Assay ◽

Human Leptospirosis ◽

Polymerase Chain

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Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2012000300002 ◽

2012 ◽

Vol 32 (3) ◽

pp. 194-198 ◽

Cited By ~ 8

Author(s):

Rodrigo Costa da Silva ◽

Veruska Maia da Costa ◽

Fabio Hiroto Shimabukuro ◽

Virgínia Bodelão Richini-Pereira ◽

Benedito Donizete Menozzi ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Bacterial Growth ◽

Agglutination Test ◽

The State ◽

Rodent Control ◽

Chain Reaction ◽

Serum Samples ◽

Leptospira Spp ◽

Positive Serum ◽

Polymerase Chain

Leptospirosis is a worldwide anthropozoonosis that infects livestock, including sheep as the carriers to other animals and humans. The present study aimed to determine the prevalence of Leptospira spp. in sheep from two slaughterhouses in the state of São Paulo, Brazil and its association with epidemiological variables. Serum samples from 182 sheep were evaluated for Leptospira spp. antibodies by microscopic agglutination test (MAT). Results indicated 34/182 (18.68%; CI95% 13.70-24.98%) positive serum samples, mainly to the serovar Copenhageni (17/34; 50%; CI95% 33.99-66.01%). Bacterial growth in the Fletcher medium was detected for 13/34 (38.24%; CI95% 23.87-55.08%) animals, and confirmed by Polymerase Chain Reaction (PCR) and sequencing for only two kidney samples from two animals. Thus, treatment and vaccination of sheep, besides rodent control, can be useful to prevent the infection in the studied region since sheep are important Leptospira spp. carriers, and its transmission to slaughterhouse workers is mainly through the manipulation of visceral tissues.

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Detection of intraocular Leptospira spp. by real‐time polymerase chain reaction in horses with recurrent uveitis in Belgium

Equine Veterinary Journal ◽

10.1111/evj.13012 ◽

2018 ◽

Vol 51 (3) ◽

pp. 299-303 ◽

Cited By ~ 8

Author(s):

A. C. Sauvage ◽

S. J. Monclin ◽

M. Elansary ◽

P. Hansen ◽

M. F. Grauwels

Keyword(s):

Polymerase Chain Reaction ◽

Real Time ◽

Chain Reaction ◽

Leptospira Spp ◽

Polymerase Chain

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Evidence of exposure to Leptospira spp. in dogs at a zoonosis control center in Brazil

Revista Acadêmica Ciência Animal ◽

10.7213/2596-2868.2020.18301 ◽

2020 ◽

Vol 18 ◽

pp. 1

Author(s):

Mírian Da Rocha Albuquerque ◽

Thalita Amaral dos Reis ◽

Katarine De Souza Rocha ◽

Jacqueline Da Silva Brito ◽

Gleiciane Schupp De Sena Mesquita ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Whole Blood ◽

Agglutination Test ◽

Microscopic Agglutination Test ◽

Chain Reaction ◽

Serum Samples ◽

Control Center ◽

Leptospira Spp ◽

Polymerase Chain ◽

Prior Contact

Evidence of exposure to Leptospira spp. in dogs housed in the kennel of the Zoonosis Control Center of Belém, Pará, Brazil, was investigated. Whole blood and serum samples from 145 dogs were investigated using the polymerase chain reaction (PCR) and microscopic agglutination test (MAT), respectively. A total of 64.14% of the dogs were found to be seropositive for Leptospira spp., with the most frequent serogroup being Djasiman (39.73%). However, PCR results revealed that all of the dogs were negative for Leptospira spp. DNA. Although the results of the study suggest the animals did not currently have leptospires in blood, they only show circulating anti-Leptospira spp. antibodies, implying prior contact with the bacteria.

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Caracterización epidemiológica de la infección por Leptospira spp. en caballos de trabajo y en personas ocupacionalmente expuestas en seis unidades de la Policía Nacional de Colombia

Biomédica ◽

10.7705/biomedica.v39i1.4475 ◽

2019 ◽

Vol 39 ◽

pp. 19-34 ◽

Cited By ~ 3

Author(s):

Juan Camilo Calderón ◽

Miryam Astudillo ◽

Marlyn H. Romero

Keyword(s):

Polymerase Chain Reaction ◽

Agglutination Test ◽

Chain Reaction ◽

Leptospira Spp ◽

Polymerase Chain

Introducción. Los caballos de trabajo de la Policía Nacional tienen un estrecho contacto con sus manejadores y la población en general durante las actividades recreativas y de patrullaje, lo cual puede favorecer la transmisión de la leptospirosis en los caballos y el personal ocupacionalmente expuesto.Objetivo. Caracterizar epidemiológicamente la leptospirosis mediante pruebas de serología, urocultivo y reacción en cadena de la polimerasa (Polymerase Chain Reaction, PCR) en caballos de trabajo y personal con riesgo ocupacional pertenecientes a seis unidades de la Policía Nacional de Colombia.Materiales y métodos. Se evaluaron 153 caballos machos castrados y 123 personas en las seis unidades en los municipios de Manizales, Pereira, Armenia, Ibagué, Tuluá y Cali. Se utilizaron tres formatos estructurados para recabar información y se obtuvieron muestras sanguíneas de las personas y de los caballos, las cuales se procesaron con la prueba de aglutinación microscópica (Macroscopic Agglutination Test, MAT) para 24 serogrupos. Se practicó el examen clínico de los caballos y se obtuvieron muestras de orina para el urocultivo y la PCR convencional.Resultados. La seroprevalencia de Leptospira spp. fue de 3,25 % (n=4) en las personas y de 85 % (n=130) en los caballos. Entre los caballos, los serogrupos Djasiman y Shermani fueron los más prevalentes. El urocultivo fue positivo en el 64,7 % (99/153) de las muestras, en tanto que los análisis de PCR fueron negativos. Se encontró una asociación estadísticamente significativa de la frecuencia de salida de las instalaciones (p=0,009) y la presencia de fauna silvestre (p=0,051) con la infección por el serogrupo Shermani. Conclusión. Las características epidemiológicas de la leptospirosis en los caballos sugieren una presentación endémica de la infección y su papel como reservorios de la bacteria; sin embargo, debe dilucidarse la patogenia de la enfermedad con estudios complementarios.

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