scholarly journals Longitudinal study of the indirect immunofluorescence and complement fixation tests for diagnosis of chagas' disease in immunosuppressed patients submitted to renal transplantation

1993 ◽  
Vol 26 (4) ◽  
pp. 211-214 ◽  
Author(s):  
José Fernando de Castro Figueiredo ◽  
Adhemar Mário Fiorillo ◽  
Agenor Spallini Ferraz
Keyword(s):  
Renal Transplantation ◽  
Chagas Disease ◽  
Indirect Immunofluorescence ◽  
Complement Fixation ◽  
Signs And Symptoms ◽  
Serological Response ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Immunosuppressed Patients

Clinical and serological follow-up of 7 patients submitted to renal transplantation and presenting positive serological reactions to Chagas 'disease before immunossupression did not show significant changes in indirect immunofluorescence and complement fixation titres for Chagas ' disease, or signs and symptoms indicating exacerbation of the disease during follow- up. In addition, 18 of 66 recipients of renal transplants considered to be non-chagasic before immunosuppression showed at least one positive result to the indirect immunofluorescence test for Chagas ' disease during the study period. The results suggest that the immunosuppression State induced in chagasic patients submitted to renal transplant did notpromoted exacerbation of the chronic infection in these patients and not interfere with the serological response of chronic chagasics, thus permitting the use of these serologic reactions for diagnostic purposes in these cases. However, the positive results ofthe indirect immunofluorescence test in non- chagasic patients indicate the needforjudicious interpretation ofthe indirect immunofluorescence test for the diagnosis of Chagas' disease in renal transplanted patients.

scholarly journals Indirect immunofluorescence(IgG and IgM) tests for toxoplasmosis on 203 persons, with no symptomatology suggesting the disease, located in the city of Rio de Janeiro. Serological follow up one to two years later.

1981 ◽  
Vol 76 (4) ◽  
pp. 397-407
Author(s):  
Maria Regina Reis Amendoeira ◽  
Sergio Gomes Coutinho
Keyword(s):  
Indirect Immunofluorescence ◽  
Rio De Janeiro ◽  
Physical Sign ◽  
Immunofluorescence Test ◽  
Group Iii ◽  
Indirect Immunofluorescence Test ◽  
Group I ◽  
One Year ◽  
The City

Clinical and serological follow up examinations were performed on 203 persons, from three to twenty years of age, from the otolaryngology department of a hospital in the city of Rio de Janeiro, with no symptomatology suggesting toxoplasmosis, but suffering from chronic tonsillitis. According to results obtained during the first indirect immunofluorescence tests, the patients were divided into following groups: Group I (non-reactive IgG and IgM), 98 persons (48.3%); Group II (1:16 ≤ IgG ≤ 1:256 and non-reactive IgM), 74 persons (36.5%); Group III (IgM ≥ 1:1024 and non-reactive IgM), 18 persons (8.8%), and Group IV (IgG and IgM reactive), 13 persons (6.4%). One to two years later, 131 (64.5%) of the 203 persons were reexamined by a second indirect immunofluorescence test. In the case of 66 persons (Group I) whose serum was non-reactive in the IgG and IgM classes during the first indirect immunofluorescence test, serum conversion was observed in aproximately 21.2%. in 65 individuals (49.6%), (Groups II, III and IV),with reactive serum in the IgG classes during the first indirect immunofluorescence test, the second reaction showed an increase in titres in 20% of the cases, a decrease in 67.7% of the cases, or no alterations in 12.3 of the cases. In the IgM class, all 131 sera were non-reactive at 116 dilution the second immunofluorescence test, including the 13 cases that had previously been reactive in the immunoglobulin class, Symptomatology suggesting toxoplasmosis was only observed in one case during the second testing, this patient's principal physical sign being hypertrophied lymph nodes. during this period, the Toxoplasma antibodies showed titres of IgG 1:32000 and non-reactive IgM, whilst one year previously, during the first test, these titres were IgG 1:1024 and IgM 1:64. Differences in the age, sex and skin coloring of patients were not statistically significant as regards alterations in the indirect immunofluorescence test titres.

Recognition of a Non-HLA-ABC Antigen Present on B and T Lymphocytes and Monocytes only Detectable with the Indirect Immunofluorescence Test

Vox Sanguinis ◽  
1979 ◽  
Vol 36 (3) ◽  
pp. 150-158
Author(s):  
F. Décary ◽  
F.W.A. Verheugt ◽  
L. van Helden-Henningheim ◽  
E. von Riesz ◽  
R. Schreuder-van Gelder ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Indirect Immunofluorescence ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Antigen Present ◽  
B And T Lymphocytes

scholarly journals Behaviour of indirect immunofluorescence test and some cerebrospinal parameters in neurocysticercosis

1987 ◽  
Vol 45 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Amauri Braga Simonetti ◽  
Jorge Teixeira
Keyword(s):  
Cerebrospinal Fluid ◽  
Indirect Immunofluorescence ◽  
False Positive ◽  
Clinical Evidence ◽  
Routine Analysis ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Immunofluorescent Test ◽  
Positive Results

Cerebrospinal fluid from 53 patients with clinical evidence of neurocysticercosis and 11 who suffered from several diseases were studied to evaluate the behaviour of indirect immunofluorescence test and some parameters of routine analysis. In neurocysticercosis there were pleocitosis in 88.7% of cases, eosinophilorrachia in 60.3%o, hyperproteinorrachia in 71.7% and hypoglucorrachia in 13.2%. The indirect immunofluorescence test was positive in 19.2% of cases but false-positive results were found when the samples showed xanthochromia or erythrocyte contamination. The authors discuss their results in comparison with those in literature and conclude that the immunofluorescent test is sensitive and useful in diagnosis of neurocysticercosis, except when the interferents previously mentioned are present.

Evaluation of the automated indirect immunofluorescence test for anti-dsDNA antibodies

2019 ◽  
Vol 498 ◽  
pp. 143-147 ◽  
Author(s):  
Shiji Wu ◽  
Feng Wang ◽  
Jin Huang ◽  
Botao Yin ◽  
Min Huang ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Dsdna Antibodies

Correlation between enzyme immunoassay and indirect immunofluorescence test for high titer of anti-ribosomal-P antibodies

2012 ◽  
Vol 413 (5-6) ◽  
pp. 650-652 ◽  
Author(s):  
Kyeong-Hee Kim ◽  
Bo-Ram Kim ◽  
Ji-Eun Kim ◽  
Kwang-Sook Woo ◽  
Jin-Yeong Han ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Indirect Immunofluorescence ◽  
High Titer ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Ribosomal P

Therapy of Alveococcosis in Man

1977 ◽  
Vol 5 (5) ◽  
pp. 367-368 ◽  
Author(s):  
C E Lüdin ◽  
K Gyr ◽  
K Karoussos
Keyword(s):  
Partial Hepatectomy ◽  
Indirect Immunofluorescence ◽  
The Other ◽  
Immunofluorescence Test ◽  
Serological Tests ◽  
Passive Haemagglutination ◽  
Indirect Immunofluorescence Test ◽  
Reliable Methods

Diagnostic, therapeutic and prognostic aspects are interpreted on the basis of two cases of Echinococcus alveolaris. Both patients had undergone partial hepatectomy and were subsequently treated chemotherapeutically. In one case dehydroemetine and mebendazole (Vermox®, Janssen), and in the other case metrifonate (Bilarcil®, Bayer A. G.) were used. The diagnosis of alveococcosis of the liver was demonstrated in one case histologically on the occasion of appendectomy, and in the other case by the indirect immunofluorescence test and passive haemagglutination. In case of suspected alveococcosis these serological tests are imperative, as they are the most reliable methods of demonstrating this disease. The latest trend in therapy aims at a combination of surgery and chemotherapy. Mebendazole seems to be promising as an anthelmintic agent for the treatment of alveococcosis. Remission of the disease was obtained in either case.

Isolation of the Causative Organism of Canine Encephalitozoonosis

1978 ◽  
Vol 15 (4) ◽  
pp. 449-460 ◽  
Author(s):  
J. A. Shadduck ◽  
R. Bendele ◽  
G. T. Robinson
Keyword(s):  
Endothelial Cells ◽  
Indirect Immunofluorescence ◽  
Renal Tubule ◽  
Causative Organism ◽  
Encephalitozoon Cuniculi ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Tubule Cells ◽  
Spontaneous Infection

Spontaneous infection with Encephalitozoon cuniculi resulted in a lethal disease in all but one of a litter of puppies in Texas. The disease was characterized by severe nonsuppurative nephritis, encephalitis and segmental vasculitis. Many protozoa were in renal tubule cells, endothelial cells and brain. The number of organisms decreased and the granulomatous character of the lesions became more prominent as the disease progressed. Sera from affected puppies and their parents reacted in an indirect immunofluorescence test with canine E. cuniculi propagated in vitro. The sera of owners of affected pups were negative.

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