scholarly journals Rabies diagnosis and serology in bats from the State of São Paulo, Brazil

2011 ◽  
Vol 44 (2) ◽  
pp. 140-145 ◽  
Author(s):  
Marilene Fernandes de Almeida ◽  
Luzia Fátima Alves Martorelli ◽  
Miriam Martos Sodré ◽  
Ana Paula Arruda Geraldes Kataoka ◽  
Adriana Ruckert da Rosa ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Sao Paulo ◽  
São Paulo ◽  
Tadarida Brasiliensis ◽  
Control Center ◽  
Glossophaga Soricina ◽  
Molossus Molossus ◽  
Immunofluorescent Test ◽  
Artibeus Lituratus ◽  
Rabies Diagnosis

INTRODUCTION: Bats are one of the most important reservoirs and vectors of the rabies virus in the world. METHODS: From 1988 to 2003, the Zoonosis Control Center in São Paulo City performed rabies diagnosis on 5,670 bats by direct immunofluorescent test and mouse inoculation test. Blood samples were collected from 1,618 bats and the sera were analyzed using the rapid fluorescent focus inhibition test to confirm rabies antibodies. RESULTS: Forty-four (0.8%) bats were positive for rabies. The prevalence of rabies antibodies was 5.9% using 0.5IU/ml as a cutoff. Insectivorous bats (69.8%) and bats of the species Molossus molossus (51.8%) constituted the majority of the sample; however, the highest prevalence of antibodies were observed in Glossophaga soricina (14/133), Histiotus velatus (16/60), Desmodus rotundus (8/66), Artibeus lituratus (5/54), Nyctinomops macrotis (3/23), Tadarida brasiliensis (3/48), Carollia perspicillata (3/9), Eumops auripendulus (2/30), Nyctinomops laticaudatus (2/16), Sturnira lilium (2/17) and Eumops perotis (1/13). The prevalence of rabies antibodies was analyzed by species, food preference and sex. CONCLUSIONS: The expressive levels of antibodies associated with the low virus positivity verified in these bats indicate that rabies virus circulates actively among them.

scholarly journals Vírus da raiva em quirópteros naturalmente infectados no Estado de São Paulo, Brasil

2007 ◽  
Vol 41 (3) ◽  
pp. 389-395 ◽  
Author(s):  
Karin Corrêa Scheffer ◽  
Maria Luiza Carrieri ◽  
Avelino Albas ◽  
Helaine Cristina Pires dos Santos ◽  
Ivanete Kotait ◽  
...  
Keyword(s):  
Sao Paulo ◽  
São Paulo ◽  
Tadarida Brasiliensis ◽  
Desmodus Rotundus ◽  
Lasiurus Cinereus ◽  
Artibeus Lituratus

OBJETIVO: Identificar as espécies de morcegos envolvidas na manutenção do ciclo da raiva, verificar a distribuição do vírus da raiva em tecidos e órgãos de morcegos e os períodos de mortalidade dos camundongos inoculados. MÉTODOS: A positividade para o vírus da raiva foi avaliada por imunofluorescência direta em morcegos de municípios do Estado de São Paulo, de abril de 2002 a novembro de 2003. A distribuição do vírus nos morcegos foi avaliada pela inoculação de camundongos e infecção de células N2A, com suspensões a 20% preparadas a partir de fragmentos de diversos órgãos e tecidos, além de cérebro e glândula salivar. A mortalidade dos camundongos foi observada diariamente, após inoculação intracerebral. RESULTADOS: Dos 4.393 morcegos pesquisados, 1,9% foram positivos para o vírus da raiva, pertencentes a dez gêneros, com predomínio de insetívoros. A média do período máximo de mortalidade dos camundongos pós-inoculação a partir de cérebros e glândulas salivares de morcegos hematófagos foi de 15,33±2,08 dias e 11,33±2,30 dias; insetívoros, 16,45±4,48 dias e 18,91±6,12 dias; e frugívoros, 12,60±2,13 dias e 15,67±4,82 dias, respectivamente. CONCLUSÕES: As espécies infectadas com o vírus da raiva foram: Artibeus lituratus, Artibeus sp., Myotis nigricans, Myotis sp., Eptesicus sp., Lasiurus ega, Lasiurus cinereus, Nyctinomops laticaudatus, Tadarida brasiliensis, Histiotus velatus, Molossus rufus, Eumops sp. e Desmodus rotundus. A pesquisa de vírus em diferentes tecidos e órgãos mostrou-se que os mais apropriados para o isolamento foram cérebro e glândulas salivares.

scholarly journals Bat rabies in the north-northwestern regions of the state of São Paulo, Brazil: 1997-2002

2006 ◽  
Vol 40 (6) ◽  
pp. 1082-1086 ◽  
Author(s):  
Elenice Maria Sequetin Cunha ◽  
Luzia Helena Queiroz da Silva ◽  
Maria do Carmo Custódio Souza Hunold Lara ◽  
Alessandra Figueiredo Castro Nassar ◽  
Avelino Albas ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Sao Paulo ◽  
The State ◽  
Southeastern Brazil ◽  
São Paulo ◽  
The North ◽  
Artibeus Lituratus ◽  
Bat Rabies

OBJECTIVE: Reports on bat rabies in Brazil are sporadic and isolated. This study aimed at describing the detection of rabies virus in bats in the state of São Paulo. METHODS: A total of 7,393 bats from 235 municipalities of the north and northwestern areas of the state of São Paulo, Southeastern Brazil, were assessed according to their morphological and morphometric characteristics from 1997 to 2002. Fluorescent antibody test and mice inoculation were used for viral identification. RESULTS: Of all samples examined, 1.3% was rabies virus positive, ranging from 0.2% in 1997 to 1.6% in 2001. There were found 98 bats infected, 87 in the urban area. Fluorescent antibody test was detected in 77 positive samples, whereas 92 produced rabies signs in mice; incubation period ranging from 4 to 23 days. In 43 cities at least one rabid bat was observed. The highest proportion (33.7%) of rabies virus was found in Artibeus lituratus. Eptesicus and Myotis were the most frequent positive species (24.5%) of the Vespertilionidae family. The species Molossus molossus and Molossus rufus showed 14.3% positive bats. There were no differences in the distribution of positive rabies between females (33; 48.5%) and males (35; 51.5%). CONCLUSIONS: Rabies-infected bats were found in environments that pose a risk to both human and domestic animal population and there is a need for actions aiming at the control of these species and public education.

scholarly journals Canine rabies epidemiology in Araçatuba and neighborhood, Northwestern São Paulo State - Brazil

2004 ◽  
Vol 37 (2) ◽  
pp. 139-142 ◽  
Author(s):  
Luzia Helena Queiroz da Silva ◽  
César Eduardo Bissoto ◽  
Ádna Cleia B. Delbem ◽  
Clara Isabel de Lucca Ferrari ◽  
Sílvia Helena Venturoli Perri ◽  
...  
Keyword(s):  
Urban Areas ◽  
Sao Paulo ◽  
São Paulo ◽  
Canine Rabies ◽  
Paulo State ◽  
São Paulo State ◽  
The Mean ◽  
Northwest Region ◽  
Epidemiological Characteristics ◽  
Rabies Diagnosis

Epidemiological characteristics of canine rabies in the northwest region of São Paulo State (Araçatuba region), Brazil, from 1993 to 1997 are presented. Out of 1,984 dogs, a total of 351 (17.7%) were positive for rabies diagnosis; 89% (312/351) of these occurred in urban areas and 85% (266/312) of the urban positive cases were among owned dogs. The mean age of the rabid dogs was 34 months and 61% were male. Aggressive behavior was observed in 77% of rabid dogs, followed by lack of coordination and paralysis (42%) and 48% of these dogs were responsible biting people or other animals. Information about vaccination status was obtained from 182 records and 51% of rabid dogs were non-vaccinated. The number of unvaccinated rabid dogs indicates a low vaccination index and this factor added to the high dog/man ratio must have contributed to the canine rabies epizootic observed in the studied area.

Genetic characterization of Rabies virus isolated from cattle between 1997 and 2002 in an epizootic area in the state of São Paulo, Brazil

2009 ◽  
Vol 144 (1-2) ◽  
pp. 215-224 ◽  
Author(s):  
Pedro Carnieli ◽  
Juliana Galera Castilho ◽  
Willian de Oliveira Fahl ◽  
Nazle Mendonça Collaço Véras ◽  
Maria do Carmo Sampaio Tavares Timenetsky
Keyword(s):  
Rabies Virus ◽  
Genetic Characterization ◽  
Sao Paulo ◽  
The State ◽  
São Paulo

scholarly journals Pathogenicity of different rabies virus isolates and protection test in vaccinated mice

2010 ◽  
Vol 52 (5) ◽  
pp. 231-236 ◽  
Author(s):  
Elenice M.S. Cunha ◽  
Alessandra F.C. Nassar ◽  
Maria do Carmo C.S.H. Lara ◽  
Eliana C.M. Villalobos ◽  
Go Sato ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Virus Isolate ◽  
Death Rates ◽  
Intramuscular Route ◽  
Canine Rabies ◽  
Commercial Vaccine ◽  
Molossus Molossus ◽  
Artibeus Lituratus ◽  
Bat Rabies ◽  
Virus Isolates

This study was aimed to evaluate and compare the pathogenicity of rabies virus isolated from bats and dogs, and to verify the efficacy of a commercial rabies vaccine against these isolates. For evaluation of pathogenicity, mice were inoculated by the intramuscular route (IM) with 500MICLD50/0.03mL of the viruses. The cross-protection test was performed by vaccinating groups of mice by the subcutaneous route and challenged through the intracerebral (IC) route. Isolates were fully pathogenic when inoculated by the IC route. When inoculated intramuscularly, the pathogenicity observed showed different death rates: 60.0% for the Desmodus rotundus isolate; 50.0% for dog and Nyctinomops laticaudatus isolates; 40.0% for Artibeus lituratus isolate; 9.5% Molossus molossus isolate; and 5.2% for the Eptesicus furinalis isolate. Mice receiving two doses of the vaccine and challenged by the IC route with the isolates were fully protected. Mice receiving only one dose of vaccine were partially protected against the dog isolate. The isolates from bats were pathogenic by the IC route in mice. However, when inoculated through the intramuscular route, the same isolates were found with different degrees of pathogenicity. The results of this work suggest that a commercial vaccine protects mice from infection with bat rabies virus isolates, in addition to a canine rabies virus isolate.

Development of a simple HPLC-DAD multi-analyte procedure and its application in cases evaluated by the Poison Control Center of São Paulo, Brazil

2018 ◽  
Vol 32 (12) ◽  
pp. e4360 ◽  
Author(s):  
Sarah C.W.S.E. Franco de Oliveira ◽  
Alexandre D. Zucoloto ◽  
Carolina D.R. de Oliveira ◽  
Edna M.M. Hernandez ◽  
Ligia V.G. Fruchtengarten ◽  
...  
Keyword(s):  
Sao Paulo ◽  
Poison Control ◽  
Poison Control Center ◽  
São Paulo ◽  
Control Center

scholarly journals Quiropterofauna da Fazenda Santo Antônio dos Ipês, Jaú, estado de São Paulo, Brasil

Biotemas ◽  
2016 ◽  
Vol 29 (1) ◽  
pp. 97 ◽  
Author(s):  
Ayesha Ribeiro Pedrozo ◽  
Luiz Antonio Costa Gomes ◽  
Moisés Guimarães ◽  
Wilson Uieda
Keyword(s):  
Sao Paulo ◽  
São Paulo ◽  
Carollia Perspicillata ◽  
Artibeus Lituratus ◽  
Muntingia Calabura

http://dx.doi.org/10.5007/2175-7925.2016v29n1p97O presente estudo registrou as espécies de morcegos que ocorrem na Fazenda Santo Antônio dos Ipês, Jaú, SP, Brasil, com a finalidade de identificar os itens alimentares consumidos por esses animais e avaliar a presença do vírus rábico nos mesmos. Vinte e uma noites de captura, distribuídas em cinco áreas da fazenda, foram realizadas utilizando redes de neblina entre novembro de 2009 e julho de 2011. Após um esforço de captura de 18217,5 m2.h, 580 morcegos de 16 espécies foram capturados. Sturnira lilium (n = 184), Artibeus lituratus (n = 134) e Carollia perspicillata (n = 126) foram as espécies mais abundantes. Dois indivíduos de Lasiurus ega foram adicionados ao inventário através de observação em abrigo, totalizando assim 17 espécies amostradas. Os itens alimentares mais consumidos foram os frutos de Solanaceae e de Piperaceae, e os frutos de Muntingia calabura os menos consumidos. Nenhum dos 132 indivíduos analisados para o vírus rábico apresentou resultado positivo. A Fazenda Santo Antônio dos Ipês é uma importante área, pois abriga a Reserva Ecológica Amadeu Botelho (uma Unidade de Conservação) que é o último resquício de Floresta Estacional Semidecidual da região de Jaú e que serve como refúgio para animais silvestres incluindo morcegos.

scholarly journals Antigenic and genotypic characterization of rabies virus isolated from bats (Mammalia: Chiroptera) from municipalities in São Paulo State, Southeastern Brazil

2017 ◽  
Vol 162 (5) ◽  
pp. 1201-1209 ◽  
Author(s):  
Benedito Donizete Menozzi ◽  
Rafael de Novaes Oliveira ◽  
Laís Moraes Paiz ◽  
Virgínia Bodelão Richini-Pereira ◽  
Helio Langoni
Keyword(s):  
Rabies Virus ◽  
Sao Paulo ◽  
Southeastern Brazil ◽  
São Paulo ◽  
Paulo State ◽  
Genotypic Characterization ◽  
São Paulo State

scholarly journals Multivariate analysis applied in dataset of Poison Control Center of São Paulo, Brazil

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sarah Eller ◽  
Alexandre Dias Zucoloto ◽  
Carolina Dizioli Rodrigues de Oliveira ◽  
Edna Maria Miello Hernandez ◽  
Ligia Veras Gimenez Fruchtengarten ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Sao Paulo ◽  
Poison Control ◽  
Poison Control Center ◽  
São Paulo ◽  
Control Center

scholarly journals Genetic characterization of rabies virus isolated from bovines and equines between 2007 and 2008, in the States of São Paulo and Minas Gerais

2010 ◽  
Vol 43 (2) ◽  
pp. 116-120 ◽  
Author(s):  
Carla Isabel Macedo ◽  
Pedro Carnieli Junior ◽  
Willian de Oliveira Fahl ◽  
Jonas Yoshitaka de Oliveira Lima ◽  
Rafael de Novaes Oliveira ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Genetic Characterization ◽  
Active Regions ◽  
Minas Gerais ◽  
Sao Paulo ◽  
Amino Acid Sequences ◽  
Biologically Active ◽  
Economic Losses ◽  
São Paulo

INTRODUCTION: Rabies is an acute disease of the central nervous system and is responsible for the deaths of thousands of humans, wild animals and livestock, particularly cattle, as well as causing major economic losses. This study describes the genetic characterization of rabies virus variants that circulate in Desmodus rotundus populations and are transmitted to herbivores. METHODS: Fifty rabies virus isolates from bovines and equines in the States of São Paulo and Minas Gerais, Brazil, were genetically characterized and compared with sequences retrieved from GenBank. RESULTS: Two clusters (I and II) with mean nucleotide identities of 99.1 and 97.6% were found. The first of these contained nearly all the samples analyzed. Lineages from other Brazilian states grouped in cluster II. CONCLUSIONS: Analysis of the amino acid sequences of the N proteins revealed the existence of genetic markers that may indicate possible variations between geographic regions, although the biologically active regions are conserved within the species over space and time.

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