Acoustic and Genetic Data Can Reduce Uncertainty Regarding Populations of Migratory Tree-Roosting Bats Impacted by Wind Energy

Wind turbine-related mortality may pose a population-level threat for migratory tree-roosting bats, such as the hoary bat (Lasiurus cinereus) in North America. These species are dispersed within their range, making it impractical to estimate census populations size using traditional survey methods. Nonetheless, understanding population size and trends is essential for evaluating and mitigating risk from wind turbine mortality. Using various sampling techniques, including systematic acoustic sampling and genetic analyses, we argue that building a weight of evidence regarding bat population status and trends is possible to (1) assess the sustainability of mortality associated with wind turbines; (2) determine the level of mitigation required; and (3) evaluate the effectiveness of mitigation measures to ensure population viability for these species. Long-term, systematic data collection remains the most viable option for reducing uncertainty regarding population trends for migratory tree-roosting bats. We recommend collecting acoustic data using the statistically robust North American Bat Monitoring Program (NABat) protocols and that genetic diversity is monitored at repeated time intervals to show species trends. There are no short-term actions to resolve these population-level questions; however, we discuss opportunities for relatively short-term investments that will lead to long-term success in reducing uncertainty.

Timing and Weather Offer Alternative Mitigation Strategies for Lowering Bat Mortality at Wind Energy Facilities in Ontario

Relatively high mortality of migratory bats at wind energy facilities has prompted research to understand the underlying spatial and temporal factors, with the goal of developing more effective mitigation approaches. We examined acoustic recordings of echolocation calls at 12 sites and post-construction carcass survey data collected at 10 wind energy facilities in Ontario, Canada, to quantify the degree to which timing and regional-scale weather predict bat activity and mortality. Rain and low temperatures consistently predicted low mortality and activity of big brown bats (Eptesicus fuscus) and three species of migratory tree bats: hoary bat (Lasiurus cinereus), eastern red bat (L. borealis), and silver-haired bat (Lasionycteris noctivagans). Bat activity occurred in waves with distinct peaks through the season; regardless of seasonal timing, most activities occurred in the first half of the night. We conclude that wind energy facilities could adopt a novel and more effective curtailment strategy based on weather and seasonal and nocturnal timing that would minimize mortality risks for bats while increasing the opportunities for power generation, relative to the mitigation strategy of increasing cut-in wind speed to 5.5 m/s.

Silence and reduced echolocation during flight are associated with social behaviors in male hoary bats (Lasiurus cinereus)

Bats are renowned for their sophisticated echolocation. However, recent research has indicated that bats may be less reliant on echolocation than has long been assumed. To test the hypothesis that bats reduce their use of echolocation to avoid eavesdropping by conspecifics, we deployed miniature tags that recorded ultrasound and accelerations on 10 wild hoary bats (Lasiurus cinereus) for one or two nights. This resulted in 997 10-s recordings. Bats switched between periods predominated by their typical high-intensity echolocation, or periods predominated by micro calls (unusually short, quiet calls), or no detectable calls (“silence”). Periods of high-intensity echolocation included high rates of feeding buzzes, whereas periods of micro calls and silence included high rates of social interactions with other bats. Bats switched back to high-intensity echolocation during actual social interactions. These data support the hypothesis that bats use reduced forms of echolocation and fly in silence to avoid eavesdropping from conspecifics, perhaps in the context of mating-related behavior. They also provide the strongest demonstration to date that bats fly for extended periods of time without the use of echolocation.

Historical effective population size of North American hoary bat (Lasiurus cinereus) and challenges to estimating trends in contemporary effective breeding population size from archived samples

Background Hoary bats (Lasiurus cinereus) are among the bat species most commonly killed by wind turbine strikes in the midwestern United States. The impact of this mortality on species census size is not understood, due in part to the difficulty of estimating population size for this highly migratory and elusive species. Genetic effective population size (Ne) could provide an index of changing census population size if other factors affecting Ne are stable. Methods We used the NeEstimator package to derive effective breeding population size (Nb) estimates for two temporally spaced cohorts: 93 hoary bats collected in 2009–2010 and an additional 93 collected in 2017–2018. We sequenced restriction-site associated polymorphisms and generated a de novo genome assembly to guide the removal of sex-linked and multi-copy loci, as well as identify physically linked markers. Results Analysis of the reference genome with psmc suggested at least a doubling of Ne in the last 100,000 years, likely exceeding Ne = 10,000 in the Holocene. Allele and genotype frequency analyses confirmed that the two cohorts were comparable, although some samples had unusually high or low observed heterozygosities. Additionally, the older cohort had lower mean coverage and greater variability in coverage, and batch effects of sampling locality were observed that were consistent with sample degradation. We therefore excluded samples with low coverage or outlier heterozygosity, as well as loci with sequence coverage far from the mode value, from the final data set. Prior to excluding these outliers, contemporary Nb estimates were significantly higher in the more recent cohort, but this finding was driven by high values for the 2018 sample year and low values for all other years. In the reduced data set, Nb did not differ significantly between cohorts. We found base substitutions to be strongly biased toward cytosine to thymine or the complement, and further partitioning loci by substitution type had a strong effect on Nb estimates. Minor allele frequency and base quality bias thresholds also had strong effects on Nb estimates. Instability of Nb with respect to common data filtering parameters and empirically identified factors prevented robust comparison of the two cohorts. Given that confidence intervals frequently included infinity as the stringency of data filtering increased, contemporary trends in Nb of North American hoary bats may not be tractable with the linkage disequilibrium method, at least using the protocol employed here.

The myobiid mites (Acariformes, Eleutherengona, Myobiidae) from Mexico: hosts, distribution and identification key for the genera and species

The family Myobiidae Mégnin, 1878 includes permanent parasites of the fur of different orders of mammals. Because the information on this family is scarce and scattered, in this work, we compile the available information on Myobiidae in Mexico. In total, we recorded 41 species (one undetermined, one a species inquirenda) belonging to 13 genera and three subfamilies, associated with four orders of mammals: Chiroptera, Didelphimorphia, Rodentia and Soricomorpha. We also reported for the first time in Mexico Eudusbabekia jimenezi (Dusbábek, 1967) and E. saguei (Dusbábek, 1967), parasitizing bats of the family Mormoopidae in the States of Colima, Nayarit and San Luis Potosí, and Acanthophthirius lasiurus (Fain & Whitaker, 1976) associated with the bat Lasiurus cinereus (Palisot de Beauvois) (family Vespertilionidae) in Ciudad de México. We recorded new associations with bat hosts: E. provirilia Morales-Malacara, Guzmán-Cornejo & López-Ortega, 2002 with Leptonycteris yerbabuenae Martínez & Villa (Phyllostomidae); E. saguei with Pteronotus davyi Gray (Mormoopidae), and Ioannela leptonycteris (Fain, 1973) with L. yerbabuenae. In this work, Zacaltepetla hoffmannae Basurto-R., 1973 is treated as a species inquirenda for the first time. We also provide an identification key to the genera and species of Myobiidae found in Mexico.

Local trends in abundance of migratory bats across 20 years

Hoary bats (Lasiurus cinereus) and silver-haired bats (Lasionycteris noctivagans) are species of conservation concern because of the documented annual mortality that occurs at wind energy facilities. Several recent studies have predicted continental-scale declines of hoary bat populations due to interactions with wind turbines. We predicted a decrease in captures at a summer site over 20 years where researchers have captured bats using generally consistent methods. We developed a hierarchical Bayesian model to estimate the relative change in the expected number of captures while controlling for time of year, temperature, and netting effort. We found no decrease in the number of captures for either species. We suggest that the lack of decrease observed at our study site may be a result of compensatory immigration, despite potential broader-scale population declines.

Species-specific environmental conditions for winter bat acoustic activity in North Carolina, United States

Low winter temperatures are a major driver of hibernation and migration in temperate North American bats. Hibernation and migration in turn affect bat mortality via white-nose syndrome and collisions with wind turbines. To describe winter bat acoustic activity across a wide temperature gradient and to understand species-specific responses to low temperatures, we recorded nightly acoustic activity of bats at 15 sites across the state of North Carolina, United States, from December through February 2016 – 2018. Bat acoustic activity was recorded at all sites during both winters. Nightly probability of bat acoustic presence regressed positively on ambient temperature. Nightly probability of presence in Lasionycteris noctivagans (silver-haired bat) and Eptesicus fuscus (big brown bat) regressed negatively on wind speed. The mean probability of presence within the same winter condition was highest for L. noctivagans, followed by E. fuscus, Perimyotis subflavus (tricolored bat), and Lasiurus cinereus (hoary bat). Differences in species’ mean body weight and roosting preference explained part of the variation of the species-specific probability of presence. Our results can be used to predict bat acoustic presence for these species across the southeastern United States in winter, and better understand the potential threats to bats such as white-nose syndrome and wind turbine interactions.

Potential foraging niche release in insectivorous bat species relatively unaffected by white-nose syndrome?

White-nose syndrome (WNS) has rendered four of Ontario’s species endangered, while leaving the other four species relatively unaffected. The causes and extent of the declines have been widely studied. The influence on remaining bat species has not. Comparing acoustic data recorded ∼10 years apart, we evaluated how species in southeastern Ontario, Canada, use different foraging habitats pre- and post-WNS detection. We observed activity declines in now-endangered species over open fields (small-footed myotis, Myotis leibii (Audubon and Bachman, 1842); little brown bat, Myotis lucifugus (Le Conte, 1831); northern myotis, Myotis septentrionalis (Trouessart, 1897); tricolored bat, Perimyotis subflavus (F. Cuvier, 1832)) and speculate that the reduction of the once most common species (M. lucifugus) may have resulted in other species searching for prey in habitat once dominated by M. lucifugus. That is, these changes may have allowed greater presence in open field and clutter or edge environments by the big brown bat (Eptesicus fuscus (Palisot de Beauvois, 1796)) and three migratory species (silver-haired bat, Lasionycteris noctivagans (Le Conte, 1831); red bat, Lasiurus borealis (Müller, 1776); hoary bat, Lasiurus cinereus (Palisot de Beauvois, 1796)). However, our results also suggest that (i) while the decline of most resident bat species due to WNS may have relaxed competition for relatively unaffected species in some, but not all habitats, that (ii) sensory and biomechanical constraints may limit prey exploitation by these less-affected bat species in these habitats.