The mosquitoes Aedes aegypti, A. triseriatus, Culex pipiens, C. quinquefasciatus, and Anopheles quadrimaculatus were all found to be efficient experimental vectors of Shope's virus-induced fibromas of cottontail rabbits, transmitting the virus during interrupted feedings as well as after long intervals from an infective meal. The reduviid bugs, Triatoma infestans, T. phyllosoma pallidipennis and Rhodnius prolixus, and the bedbug, Cimex lectularius, were also capable of transmitting fibroma by interrupted or delayed feeding.Evidence from various types of experiments indicated that arthropod transmission is mechanical, the virus being extremely stable in the insects. Some experiments did indicate the possibility of virus proliferation. Although mosquitoes did seem to serve as ‘flying pins’ when transmitting virus by interrupted feeding, they certainly were distinctive in that they maintained their ability to transmit for very long periods of time. To transmit fibromas, arthropods actually must draw virus up between the stylets of the mouthparts; mosquitoes were unable to transmit by feeding through skin moistened with a suspension of fibroma virus or by feeding subsequent to having their mouthparts painted with a virus suspension.While cottontail tumours at peak virus titres are always infective for suitable insects, the fibromas of adult domestic rabbits generally are not infective, even though the virus titre is equivalent. However, the tumours of suckling domestic rabbits do become infective for insects.
The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymph
