scholarly journals Seeds enriched with phosphorus and molybdenum improve the contribution of biological nitrogen fixation to common bean as estimated by 15n isotope dilution

2010 ◽  
Vol 34 (4) ◽  
pp. 1093-1101 ◽  
Author(s):  
Eduardo Chagas ◽  
Adelson Paulo Araújo ◽  
Bruno José Rodrigues Alves ◽  
Marcelo Grandi Teixeira
Keyword(s):  
Common Bean ◽  
N2 Fixation ◽  
15N Isotope Dilution ◽  
15N Isotope ◽  
Mineral N ◽  
N Accumulation ◽  
Biological N2 Fixation ◽  
Soil P ◽  
P Concentration ◽  
The Common

Seeds with a high concentration of P or Mo can improve the growth and N accumulation of the common bean (Phaseolus vulgaris L.), but the effect of enriched seeds on biological N2 fixation has not been established yet. This study aimed to evaluate the effect of seeds enriched with P and Mo on growth and biological N2 fixation of the common bean by the 15N isotope dilution technique. An experiment was carried out in pots in a 2 x 3 x 2 x 2 factorial design in randomized blocks with four replications, comprising two levels of soil applied P (0 and 80 mg kg-1), three N sources (without N, inoculated with rhizobia, and mineral N), two seed P concentrations (low and high), and two seed Mo concentrations (low and high). Non-nodulating bean and sorghum were used as non-fixing crops. The substrate was 5.0 kg of a Red Latosol (Oxisol) previously enriched with 15N and mixed with 5.0 kg of sand. Plants were harvested 41 days after emergence. Seeds with high P concentration increased the growth and N in shoots, particularly in inoculated plants at lower applied P levels. Inoculated plants raised from high P seeds showed improved nodulation at both soil P levels. Higher soil P levels increased the percentage of N derived from the atmosphere (%Ndfa) in bean leaves. Inoculation with the selected strains increased the %Ndfa. High seed P increased the %Ndfa in inoculated plants at lower soil P levels. High seed Mo increased the %Ndfa at lower soil P levels in plants that did not receive inoculation or mineral N. It is concluded that high seed P concentration increases the growth, N accumulation and the contribution of the biological N2 fixation in the common bean, particularly in inoculated plants grown at lower soil P availability.

scholarly journals Nodulation, contribution of biological N2 fixation, and productivity of the common bean (Phaseolus vulgaris L.) inoculated with rhizobia isolates

2017 ◽  
Vol 11 (06) ◽  
pp. 644-651 ◽  
Author(s):  
Leniany Patrícia Moreira ◽  
◽  
Ana Paula Santos Oliveira ◽  
Enderson Petrônio de Brito Ferreira ◽  
◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
N2 Fixation ◽  
Phaseolus Vulgaris L ◽  
Biological N2 Fixation ◽  
The Common

scholarly journals Nitrogen Pools in Tropical Plantations of N2-Fixing and Non-N2-Fixing Legume Trees under Different Tree Stand Densities

Nitrogen ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 86-98
Author(s):  
Kelly Nery Bighi ◽  
Ranieri Ribeiro Paula ◽  
Marcos Vinícius Winckler Caldeira ◽  
Diego Lang Burak ◽  
Eduardo de Sá Mendonça ◽  
...  
Keyword(s):  
N2 Fixation ◽  
Stand Density ◽  
N Accumulation ◽  
Biological N2 Fixation ◽  
Nitrogen Acquisition ◽  
Nitrogen Pools ◽  
Acquisition Strategies ◽  
Tropical Plantations ◽  
Schizolobium Parahyba ◽  
Slow Growing

We investigated the nitrogen pools in monocultures of legume species widely used in reforestation in Brazil that have contrasting growth and nitrogen acquisition strategies. The plantations were established with the slow-growing and N2-fixing tree Anadenanthera peregrina var. peregrina, and the fast-growing and non-fixing tree Schizolobium parahyba var. amazonicum. The measurements of N pools in the tree biomass and the soil followed standard methods and were carried out on 54 experimental plots. The N2 fixation pools were evaluated by abundance natural of 15N and the N accretion methods. The soil N content was of similar magnitude between species and stand densities. The species showed similar amounts of N in the biomass, but divergent patterns of N accumulation, as well as the 15N signature on the leaves. S. parahyba accumulated most N in the stem, while A. peregrina accumulated N in the roots and leaves. However, the N accumulation in biomass of A. peregrina stand was less constrained by environment than in S. parahyba stands. The percentage of N derived from N2 fixation in A. peregrina stands decreased with the increase of stand density. The biological N2 fixation estimates depended on the method and the response of tree species to environment.

scholarly journals Growth and yield of common bean cultivars at two soil phosphorus levels under biological nitrogen fixation

2000 ◽  
Vol 35 (4) ◽  
pp. 809-817 ◽  
Author(s):  
ADELSON PAULO ARAÚJO ◽  
MARCELO GRANDI TEIXEIRA ◽  
DEJAIR LOPES DE ALMEIDA
Keyword(s):  
Common Bean ◽  
N2 Fixation ◽  
Soil Phosphorus ◽  
Block Design ◽  
Growth And Yield ◽  
Yield Potential ◽  
Genotypic Differences ◽  
Biological N2 Fixation ◽  
Randomized Block Design ◽  
P Supply

The genotypic differences on growth and yield of common bean (Phaseolus vulgaris L.) in response to P supply were evaluated in a field experiment under biological N2 fixation. Eight cultivars were grown at two levels of applied P (12 and 50 kg ha-1 of P -- P1 and P2 respectively), in randomized block design in factorial arrangement. Vegetative biomass was sampled at three ontogenetic stages. The effects of genotype and phosphorus were significant for most traits, but not the genotype ´ phosphorus interaction. The cultivars presented different patterns of biomass production and nutrient accumulation, particularly on root system. At P1, P accumulation persisted after the beginning of pod filling, and P translocation from roots to shoots was lower. The nodule senescence observed after flowering might have reduced N2 fixation during pod filling. The responses of vegetative growth to the higher P supply did not reflect with the same magnitude on yield, which increased only 6% at P2; hence the harvest index was lower at P2. The cultivars with highest yields also presented lower grain P concentrations. A sub-optimal supply of N could have limited the expression of the yield potential of cultivars, reducing the genotypic variability of responses to P levels.

Evaluation of reference plants by labelling with 35S in the estimation of N2 fixation by 15N isotope dilution

1992 ◽  
Vol 145 (2) ◽  
pp. 177-185 ◽  
Author(s):  
S. D. Hamilton ◽  
P. M. Chalk ◽  
C. J. Smith ◽  
P. Hopmans
Keyword(s):  
Isotope Dilution ◽  
N2 Fixation ◽  
15N Isotope Dilution ◽  
15N Isotope ◽  
Reference Plants

Field estimation of N2 fixation by Acacia spp. using 15N isotope dilution and labelling with 35S

1993 ◽  
Vol 56 (1-4) ◽  
pp. 297-313 ◽  
Author(s):  
S.D. Hamilton ◽  
P. Hopmans ◽  
P.M. Chalk ◽  
C.J. Smith
Keyword(s):  
Isotope Dilution ◽  
N2 Fixation ◽  
15N Isotope Dilution ◽  
15N Isotope ◽  
Field Estimation

Comparison of growth and N accumulation of common bean (Phaseolus vulgaris L.) cv. OAC Rico and its two nodulation mutants, R69 and R99

1996 ◽  
Vol 76 (1) ◽  
pp. 73-83 ◽  
Author(s):  
Steven J. Shirtliffe ◽  
J. Kevin Vessey ◽  
B. R. Buttery ◽  
S. J. Park
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Rhizobium Leguminosarum ◽  
Field Measurements ◽  
Wild Type ◽  
Phaseolus Vulgaris L ◽  
Mineral N ◽  
N Accumulation ◽  
Nodulation Mutants ◽  
Wild Type Parent

Nodulation mutants are present in many species of legumes and have potential as ideal reference crops in field measurements of N2 fixation. The objective of this experiment was to characterize the growth, development, mineral-N accumulation, and N2 fixation of two nodulation mutants of common bean (Phaseolus vulgaris L.), R69 (nod+/fix−) and R99 (nod−) in comparison with the wild type parent OAC Rico. The mutants were incapable of N2 fixation. With dual dependence on N2 fixation and [Formula: see text] as N sources, OAC Rico accumulated more N than R69 and R99. However, when supplied with mineral [Formula: see text] sufficient to prevent N2 fixation in OAC Rico, its growth and N accumulation did not differ from those of the mutants. There were differences in internode length that resulted in different heights among the three lines. The addition of silver to the nutrient solution failed to restore nodulation to R99. As a result, it was determined that the nod− phenotype is not a result of an ethylene-related mutation. The nodulation mutants R69 and R99 appear to be good choices as reference crops for common bean in N2 fixation studies. Key words: Common bean, N accumulation, N2 fixation, nodulation mutants, Phaseolus vulgaris, Rhizobium leguminosarum bv. phaseoli

scholarly journals Seeds with high molybdenum concentration improved growth and nitrogen acquisition of rhizobium-inoculated and nitrogen-fertilized common bean plants

2013 ◽  
Vol 37 (2) ◽  
pp. 367-378 ◽  
Author(s):  
Fernanda Fátima Delgado Almeida ◽  
Adelson Paulo Araújo ◽  
Bruno José Rodrigues Alves
Keyword(s):  
Nitrate Reductase ◽  
Common Bean ◽  
N2 Fixation ◽  
Nitrogenase Activity ◽  
N Fertilizer ◽  
Growth Stages ◽  
Mineral N ◽  
N Sources ◽  
Bean Plants ◽  
Common Bean Plants

Seeds of common bean (Phaseolus vulgaris) with high molybdenum (Mo) concentration can supply Mo plant demands, but to date no studies have concomitantly evaluated the effects of Mo-enriched seeds on plants inoculated with rhizobia or treated with N fertilizer. This work evaluated the effects of seed Mo on growth and N acquisition of bean plants fertilized either by symbiotic N or mineral N, by measuring the activities of nitrogenase and nitrate reductase and the contribution of biological N2 fixation at different growth stages. Seeds enriched or not with Mo were sown with two N sources (inoculated with rhizobia or fertilized with N), in pots with 10 kg of soil. In experiment 1, an additional treatment consisted of Mo-enriched seeds with Mo applied to the soil. In experiment 2, the contribution of N2 fixation was estimated by 15N isotope dilution. Common bean plants grown from seeds with high Mo concentration flowered one day earlier. Seeds with high Mo concentration increased the leaf area, shoot mass and N accumulation, with both N sources. The absence of effects of Mo application to the soil indicated that Mo contents of Mo-enriched seeds were sufficient for plant growth. Seeds enriched with Mo increased nitrogenase activity at the vegetative stage of inoculated plants, and nitrate reductase activity at late growth stages with both N sources. The contribution of N2 fixation was 17 and 61 % in plants originating from low- or high-Mo seeds, respectively. The results demonstrate the benefits of sowing Mo-enriched seeds on growth and N nutrition of bean plants inoculated with rhizobia or fertilized with mineral N fertilizer.

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