Carbon sources and polyethylene glycol on soybean somatic embryo conversion

Somatic embryogenesis is an efficient method for the production of target cells for soybean genetic transformation. However, this method still offers low percentages of plant regeneration, and perhaps is related to the maturation process and high morphological abnormalities of the matured embryos. This study aimed to identify a maturation medium that could contribute to the outcome of more efficient plant regeneration results. Embryogenic clusters, derived from cotyledons of immature seeds of the soybean cultivars Bragg and IAS5, were used as starting material for embryos development. Different maturation media were tested by using 6% maltose, 3% sucrose or 6% sucrose, combined with or without 25 g L-1 of the osmotic regulator polyethylene glycol (PEG-8000). The histodifferentiated embryos were quantified and classified in morphological types. Percentages of converted embryos were analyzed. Cultivar Bragg resulted in higher matured embryo quantities, but lower percentages were obtained for the conversion in comparison to cultivar IAS5. While the addition of PEG did not affect the number of embryos converted into plants, 6% sucrose enhanced the conversion percent significantly.

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Somatic embryogenesis of the hybrid Abies cilicica × Abies cephalonica

Journal of Forest Science ◽

10.17221/54/2010-jfs ◽

2011 ◽

Vol 57 (No. 9) ◽

pp. 401-408 ◽

Cited By ~ 3

Author(s):

J. Korecký ◽

J. Vítámvás

Keyword(s):

Somatic Embryogenesis ◽

Native Species ◽

Zygotic Embryos ◽

Nutrient Media ◽

Abies Cephalonica ◽

Primary Explants ◽

Maturation Medium ◽

Immature Seeds ◽

Murashige And Skoog Medium ◽

Initiation Frequency

The interspecific hybrid fir Abies cilicica × Abies cephalonica is based on native species Abies cephalonica (Loud.) and Abies cilicica (Ant. et Kotschy) Carr. Many variants of nutrient media have been tested. The medium BAP at a concentration of 1 mg·l–1, 1,000 mg·l–1 myo-inositol, 500 mg·l–1 glutamine, and 1,000 mg·l–1 casein was found to be the most suitable initiation medium for somatic embryogenesis of this hybrid. Embryogenic suspensor mass was produced from immature and mature primary explants. The initiation frequency of ESM was dependent on the composition of nutrient medium, plant growth regulators, and time of seeds collection. The ESM initiation from immature seeds was higher in comparison with mature zygotic embryos, but the results were not significantly different. As the most suitable maturation medium was evaluated the medium based on Murashige and Skoog medium which contained 10 mg·l–1 abscisic acid, 100 g·l–1 PEG and 40 g·l–1 maltose (MM 2 medium).

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Somatic embryogenesis in Norway spruce: Anatomical study of embryo development and influence of polyethylene glycol on maturation process

Plant Physiology and Biochemistry ◽

10.1016/s0981-9428(99)80036-9 ◽

1999 ◽

Vol 37 (3) ◽

pp. 209-221 ◽

Cited By ~ 40

Author(s):

Hana Svobodová ◽

Jana Albrechtová ◽

Lucie Kumstýřová ◽

Helena Lipavská ◽

Martin Vágner ◽

...

Keyword(s):

Somatic Embryogenesis ◽

Polyethylene Glycol ◽

Norway Spruce ◽

Embryo Development ◽

Anatomical Study ◽

Maturation Process

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Somatic embryogenesis and plant regeneration from immature seeds of Magnolia obovata Thunberg

Plant Biotechnology Reports ◽

10.1007/s11816-007-0037-0 ◽

2007 ◽

Vol 1 (4) ◽

pp. 237-242 ◽

Cited By ~ 5

Author(s):

Yong Wook Kim ◽

So Young Park ◽

In Sun Park ◽

Heung Kyu Moon

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Magnolia Obovata ◽

Immature Seeds

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Screening of Brazilian soybean genotypes with high potential for somatic embryogenesis and plant regeneration

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2010000700012 ◽

2010 ◽

Vol 45 (7) ◽

pp. 715-720 ◽

Cited By ~ 4

Author(s):

Annette Droste ◽

Anelise Machado da Silva ◽

Iliane Freitas de Souza ◽

Beatriz Wiebke-Strohm ◽

Lauro Bücker-Neto ◽

...

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Good Alternative ◽

High Potential ◽

Embryo Induction ◽

Regenerated Plants ◽

Maturation Medium ◽

Secondary Embryo ◽

Soybean Genotypes

The aim of this work was to identify Brazilian soybean (Glycine max) genotypes with potential to respond to in vitro culture stimuli for primary somatic embryo induction, secondary embryo proliferation and plant regeneration. Differences among eight tested cultivars were observed at each stage. Two cultivars, IAS-5 and BRSMG 68 Vencedora, were selected for the evaluation of the capacity for embryo differentiation and plant regeneration. These cultivars had high embryo induction frequencies, repetitive embryogenic proliferation, and low precocious embryo germination in the initial experiment. The effect of abscisic acid (ABA) and charcoal addition on plant regeneration was investigated. The addition of ABA to proliferation medium and of ABA and activated charcoal to maturation medium increased embryo differentiation rates, which resulted in a higher number of regenerated plants. The BRSMG 68 Vencedora cultivar was found to have a high potential for embryo induction, embryo proliferation and plant regeneration. The potential of this cultivar for somatic embryogenesis was similar to that observed for cultivar IAS-5, which is currently used for soybean transformation in Brazil. BRSMG 68 Vencedora may be a good alternative genotype for soybean genetic engineering via somatic embryogenesis protocols.

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