Atividade celulolítica de fungos isolados de bagaço de cana-de-açúcar e madeira em decomposição

O objetivo deste trabalho foi identificar isolados de fungos a partir de bagaço de cana-de-açúcar e madeira em decomposição e avaliar a sua atividade celulolítica em bagaço de cana. Cinco isolados foram avaliados, tendo-se como referências os fungos Trichoderma reesei QM9414 e T. reesei RUT C30. A atividade celulolítica foi estimada pela capacidade hidrolítica do extrato enzimático dos fungos cultivados em bagaço de cana sobre os substratos papel de filtro (atividade celulolítica total) e carboximetilcelulose sódica (atividade da endoglucanase). Os isolados foram identificados pela análise molecular da região 26S rDNA. Os gêneros Paecilomyces, Aspergillus, Acremonium/Penicillium e Trichoderma foram identificados. Embora T. reesei QM9414 tenha apresentado a mais alta atividade celulolítica total, alguns isolados também apresentaram alta atividade de endoglucanase. A biodiversidade, em nichos como bagaço de cana-de-açúcar, pode fornecer linhagens de fungos celulolíticos com grande potencial biotecnológico.

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Action of Trichoderma reesei QM9414 and C30 cellulase on substrates with varying crystallinity

Enzyme and Microbial Technology ◽

10.1016/0141-0229(83)90006-6 ◽

1983 ◽

Vol 5 (5) ◽

pp. 345-351 ◽

Cited By ~ 7

Author(s):

W.L. Marsden ◽

N.W. Dunn ◽

P.P. Gray

Keyword(s):

Trichoderma Reesei ◽

Trichoderma Reesei Qm9414

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On-site integrated production of cellulases and β-glucosidases by Trichoderma reesei Rut C30 using steam-pretreated sugarcane bagasse

10.1101/461012 ◽

2018 ◽

Cited By ~ 3

Author(s):

Marcella Fernandes de Souza ◽

Elba Pinto da Silva Bon ◽

Ayla Sant’ Ana da Silvab

Keyword(s):

Carbon Source ◽

Lignocellulosic Biomass ◽

Trichoderma Reesei ◽

Sugarcane Bagasse ◽

Enzyme Preparation ◽

Integrated Approach ◽

Aspergillus Awamori ◽

Pretreated Sugarcane Bagasse ◽

Hydrolysis Of ◽

Rut C30

AbstractThe high cost of commercial cellulases still hampers the economic competitiveness of the production of fuels and chemicals from lignocellulosic biomasses. This cost may be decreased by the on-site production of cellulases with the integrated use of the lignocellulosic biomass as carbon source. This integrated approach was evaluated in the present study whereby steam-pretreated sugarcane bagasse (SPSB) was used as carbon source for the production of cellulases by Trichoderma reesei Rut C30 and the produced enzymes were subsequently used for SPSB hydrolysis. An enzyme preparation with a high cellulase activity, of 1.93 FPU/mL, was obtained, and a significant β-glucosidase activity was achieved in buffered media, indicating the importance of pH control during enzyme production. The hydrolysis of SPSB with the laboratory-made mixture resulted in a glucose yield of 80%, which was equivalent to those observed for control experiments using commercial enzymes. Even though the supplementation of this mixture with external β-glucosidase from Aspergillus awamori was found to increase the initial hydrolysis rates, it had no impact on the final hydrolysis yield. It was shown that SPSB is a promising carbon source for the production of cellulases and β-glucosidases by T. reesei Rut C30 and that the enzyme preparation obtained is effective for the hydrolysis of SPSB, supporting the on-site integrated approach to decrease the cost of the enzymatic hydrolysis of lignocellulosic biomass.

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