Genetic linkage map of Phaseolus vulgaris and identification of QTLs responsible for resistance to Xanthomonas axonopodis pv. phaseoli

Common bean (Phaseolus vulgaris) cultivars with a high degree of resistance to Xanthomonas axonopodis pv. phaseoli (Xap) are not available in Brazil. Despite many studies, a low degree of resistance to Xap continues to exist due to its complex genetic inheritance, which is not well known. The objectives of this research were to complement a common bean genetic map based on the cross between a susceptible genotype 'HAB-52' and a resistant genotype 'BAC-6', and to map and analyze genomic regions (quantitative trait loci – QTLs) related to Xap resistance. Eleven linkage groups were determined using 143 RAPD markers, covering 1,234.5 cM of the genome. This map was used to detect QTLs associated with Xap resistance on leaves and pods. The averages of disease severity on leaves (represented by the transformed disease index – TDI) and pods (represented by the diameter of lesion on pods – DLP) were added to the data of the linkage map. Five TDI QTLs and only one LDP QTL were detected. The TDI QTLs were placed in the A, B, G and J linkage groups, with phenotypic variations ranging from 12.7 to 71.6%. The DLP QTL explained 12.9% of the phenotypic variation and was mapped in a distinct linkage group. These results indicate that there are different genes involved in the control of resistance on leaves and pods.

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Genome conservation among three legume genera detected with DNA markers

Genome ◽

10.1139/g95-122 ◽

1995 ◽

Vol 38 (5) ◽

pp. 928-937 ◽

Cited By ~ 68

Author(s):

S. R. Boutin ◽

N. D. Young ◽

T. C. Olson ◽

Z.-H. Yu ◽

C. E. Vallejos ◽

...

Keyword(s):

Glycine Max ◽

Common Bean ◽

Dna Markers ◽

Marker Density ◽

Linkage Maps ◽

Linkage Groups ◽

Comparative Maps ◽

Genomic Regions ◽

High Degree ◽

Linkage Conservation

A set of 219 DNA clones derived from mungbean (Vigna radiata), cowpea (V. unguiculata), common bean (Phaseolus vulgaris), and soybean (Glycine max) were used to generate comparative linkage maps among mungbean, common bean, and soybean. The maps allowed an assessment of linkage conservation and collinearity among the three genomes. Mungbean and common bean, both of the subtribe Phaseolinae, exhibited a high degree of linkage conservation and preservation of marker order. Most linkage groups of mungbean consisted of only one or two linkage blocks from common bean (and vice versa). The situation was significantly different with soybean, a member of the subtribe Glycininae. Mungbean and common bean linkage groups were generally mosaics of short soybean linkage blocks, each only a few centimorgans in length. These results suggest that it would be fruitful to join maps of mungbean and common bean, while knowledge of conserved genomic blocks would be useful in increasing marker density in specific genomic regions for all three genera. These comparative maps may also contribute to enhanced understanding of legume evolution.Key words: RFLP, gene mapping, Phaseolus, Glycine, Vigna.

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Comparison of Seed Transmission and Survival of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans in Common Bean Seeds

Plant Health Progress ◽

10.1094/php-2013-0923-01-br ◽

2013 ◽

Vol 14 (1) ◽

pp. 41 ◽

Cited By ~ 1

Author(s):

Y. He ◽

G. P. Munkvold

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Bacterial Blight ◽

Disease Incidence ◽

Seed Transmission ◽

Common Bacterial Blight ◽

Phaseolus Vulgaris L ◽

Transmission Frequency ◽

Xanthomonas Axonopodis ◽

Seed Health

Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin (Xap) and Xanthomonas fuscans subsp. fuscans Schaad et al. (Xff) cause indistinguishable symptoms known as common bacterial blight of bean (Phaseolus vulgaris L.). These results confirm a higher disease incidence and seed transmission frequency of Xff compared to Xap and reinforce the need for seed health tests that can differentiate the two species. Accepted for publication 21 August 2013. Published 23 September 2013.

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Mapping genetic factors affecting the reaction to Xanthomonas axonopodis pv. phaseoli in Phaseolus vulgaris L. under field conditions

Genome ◽

10.1139/g01-099 ◽

2001 ◽

Vol 44 (6) ◽

pp. 1046-1056 ◽

Cited By ~ 19

Author(s):

B Tar'an ◽

T E Michaels ◽

K P Pauls

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Length Polymorphism ◽

Bacterial Blight ◽

Genetic Factors ◽

Fragment Length Polymorphism ◽

Segregation Ratio ◽

Common Bacterial Blight ◽

Phaseolus Vulgaris L ◽

Xanthomonas Axonopodis

The objectives of the present study were to evaluate the field effects of Xanthomonas axonopodis pv. phaseoli (Xap), which causes common bacterial blight (CBB) on common bean (Phaseolus vulgaris L.), and to identify genetic factors for resistance to CBB using a linkage map constructed with random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP) markers. One hundred and forty-two F2:4 lines, derived from a cross between 'OAC Seaforth' and 'OAC 95-4', and the parents were evaluated for their field reaction to CBB. In the inoculated plots, the reaction to CBB was negatively correlated with seed yield, days to maturity, plant height, hypocotyl diameter, pods per plant, and harvest index. A reduction in seed yield and its components was observed when disease-free and CBB-inoculated plots were compared. The broad-sense heritability estimate of the reaction to CBB was 0.74. The disease segregation ratio was not significantly different from the expected segregation ratio for a single locus in an F2 generation. The major gene for CBB resistance was localized on linkage group (LG) G5. A simple interval mapping procedure identified three genomic regions associated with the reaction to CBB. One quantitative trait loci (QTL), each on LG G2 (BNG71DraI), G3 (BNG21EcoRV), and G5 (PHVPVPK-1) explained 36.3%, 10.2%, and 42.2% of the phenotypic variation for the reaction to CBB, respectively. Together, these loci explained 68.4% of the phenotypic variation. The relative positions of these QTL on the core common bean map and their comparison with the previous QTL for CBB resistance are discussed.Key words: common bean, molecular markers, common bacterial blight.

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Marker-based linkage map of Andean common bean (Phaseolus vulgaris L.) and mapping of QTLs underlying popping ability traits

BMC Plant Biology ◽

10.1186/1471-2229-12-136 ◽

2012 ◽

Vol 12 (1) ◽

pp. 136 ◽

Cited By ~ 17

Author(s):

Fernando J Yuste-Lisbona ◽

Marta Santalla ◽

Carmen Capel ◽

Manuel García-Alcázar ◽

María De La Fuente ◽

...

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Linkage Map ◽

Phaseolus Vulgaris L

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Construction of an intraspecific integrated linkage map of pepper using molecular markers and doubled-haploid progenies

Genome ◽

10.1139/g95-014 ◽

1995 ◽

Vol 38 (1) ◽

pp. 112-121 ◽

Cited By ~ 94

Author(s):

Véronique Lefebvre ◽

Alain Palloix ◽

Carole Caranta ◽

Edmond Pochard

Keyword(s):

Linkage Map ◽

Capsicum Annuum ◽

Doubled Haploid ◽

Linkage Groups ◽

Hypersensitive Resistance ◽

Integrated Linkage Map ◽

New Genes ◽

Rflp Rapd ◽

Pepper Genome ◽

Genomic Regions

An integrated molecular linkage map of pepper (Capsicum annuum L.), including mainly RFLP and RAPD markers, has been constructed by alignment of three intraspecific linkage maps generated by segregating doubled-haploid progenies. A total of 85 markers covered approximately 820 cM in 14 linkage groups. Four linkage groups were assigned to 4 chromosomes. Two new genes of agronomic interest were located: L controlling hypersensitive resistance to TMV and up controlling the erect habit of the fruits. The C gene controlling the fruit pungency was more precisely located. This map is estimated to represent from 36 to 59% of the total pepper genome. An examination of segregation data has revealed several genomic regions with aberrant segregation ratios often favouring the agronomic big-fruited parents, particularly in crosses involving the exotic parent CM334, suggesting that these genome regions are subjected to selection during the process of doubled-haploid production. The suitability of doubled-haploid progenies for mapping projects and the differences observed between this intraspecific integrated map with earlier published interspecific pepper maps are discussed.Key words: Capsicum annuum, RFLP, RAPD, integrated linkage map, doubled-haploid progenies.

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Genomic Analysis of Chromosomal Regions Introgressed from Tepary Bean Associated with CBB Resistance in Phaseolus. vulgaris L.

HortScience ◽

10.21273/hortsci.32.3.544d ◽

1997 ◽

Vol 32 (3) ◽

pp. 544D-544

Author(s):

Geunhwa Jung ◽

Paul Skroch ◽

James Nienhuis ◽

Dermot Coyne

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Interspecific Cross ◽

Genomic Analysis ◽

Bacterial Disease ◽

Tepary Bean ◽

Genomic Interval ◽

Recombinant Inbred Population ◽

Pedigree Selection ◽

Genomic Regions

One of the highest levels of common bacterial blight (CBB) resistance identified in Phaseolus vulgaris is found in XAN-159, which was developed for leaf resistance to CBB through six generations of pedigree selection of progenies derived from the interspecific cross [(`Pinto UI 114' × PI 319441) × P. acutifolius PI 319443] × `Masterpiece'. A RAPD genetic linkage map was previously constructed in a recombinant inbred population derived from the common bean cross PC-50 × XAN-159 for identification of genomic regions associated with bacterial disease resistance in XAN-159. To confirm that chromosomal regions associated with CBB resistance in XAN-159 were introgressed from tepary bean, we investigated the parentage of each genomic interval in XAN-159 by studying the genomic constitutions of the four different parents involved in the pedigree. The results indicate that all genomic regions associated with CBB resistance contain intervals derived exclusively from tepary bean. The uniqueness of marker polymorphisms associated with resistance to CBB in XAN-159 will allow the application of marker assisted selection for these resistance genes in most populations of common bean.

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Occurrence and Importance of Xanthomonas axonopodis pv. Phaseoli in Common Bean (Phaseolus vulgaris L) Seed Produced under Different Seed Production System in Central Rift Valley of Ethiopia

Journal of Plant Pathology & Microbiology ◽

10.4172/2157-7471.1000406 ◽

2017 ◽

Vol 08 (04) ◽

Author(s):

Leta A ◽

Lamessa F ◽

Ayana G

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Seed Production ◽

Production System ◽

Rift Valley ◽

Phaseolus Vulgaris L ◽

Xanthomonas Axonopodis

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Integration of Brassica A genome genetic linkage map between Brassica napus and B. rapa

Genome ◽

10.1139/g07-113 ◽

2008 ◽

Vol 51 (3) ◽

pp. 169-176 ◽

Cited By ~ 38

Author(s):

Keita Suwabe ◽

Colin Morgan ◽

Ian Bancroft

Keyword(s):

Molecular Markers ◽

Linkage Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Linkage Groups ◽

Indel Markers ◽

A Genome ◽

High Level ◽

High Degree ◽

Genetic Integration

An integrated linkage map between B. napus and B. rapa was constructed based on a total of 44 common markers comprising 41 SSR (33 BRMS, 6 Saskatoon, and 2 BBSRC) and 3 SNP/indel markers. Between 3 and 7 common markers were mapped onto each of the linkage groups A1 to A10. The position and order of most common markers revealed a high level of colinearity between species, although two small regions on A4, A5, and A10 revealed apparent local inversions between them. These results indicate that the A genome of Brassica has retained a high degree of colinearity between species, despite each species having evolved independently after the integration of the A and C genomes in the amphidiploid state. Our results provide a genetic integration of the Brassica A genome between B. napus and B. rapa. As the analysis employed sequence-based molecular markers, the information will accelerate the exploitation of the B. rapa genome sequence for the improvement of oilseed rape.

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The Dark Corona Character in Seedcoats of Common Bean Cosegregates with the Pink Flower Allele vlae

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.120.3.520 ◽

1995 ◽

Vol 120 (3) ◽

pp. 520-522 ◽

Cited By ~ 6

Author(s):

Mark J. Bassett

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Linkage Map ◽

Pleiotropic Effect ◽

Phaseolus Vulgaris L ◽

The Third ◽

Pink Flower ◽

Tight Linkage

Crosses were made with two common bean (Phaseolus vulgaris L.) parents that have pink flowers (vlae/-) and mineral-brown seedcoats with dark corona, viz., vlae BC3 5-593 (derived from Lamprecht V0491) and F3 vlae dark corona (derived from Lamprecht M0048). The third parent v BC2 5-593 had white flowers (v/v) and mineral-brown seedcoats without dark corona (derived from Lamprecht M0056). The F2 progenies of the crosses v BC2 5-593 × vlae BC3 5-593 and F3vlae dark corona × v BC2 5-593 segregated for only two phenotypic classes: either pink flowers and seeds with dark corona or white flowers and seeds without dark corona. Thus, it was demonstrated that the dark corona character (Cor) is either tightly linked to vlae (<4 map units) or is a pleiotropic effect of vlae. Pleiotropy is more probable, but tight linkage cannot be ruled out. A linkage of 15 map units between Cor and R (currently, R is known to be tightly linked with C) reported by Lamprecht was not found by subsequent authors, and the linkage map of common bean should be revised accordingly, i.e., no linkage exists between V (Cor) and C.

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