Utilization of microsatellite markers to form families of "pejerrey" Odontesthes bonariensis in a genetic breeding program

Fusariosis and base rot, caused by the fungi Fusarium oxysporum f. sp. passiflorae and F. solani, respectively, seriously damage the cultivation of sour passion fruit in Brazil. This work aims to obtain and evaluate F1 hybrids of Passiflora spp. wild plants resistant to fusariosis and base rot using genotypes of P. edulis (commercial species) and certify interspecific hybridizations using microsatellite markers. Hybridizations were performed using a P. edulis female parent and P. nitida and P. mucronata male parents for crosses aiming fusariosis resistance, and P. nitida, P. cincinnata and P. quadrangularis for hybridizations aiming tolerance to base rot. 35 microsatellite markers were used to confirm hybridization. The washed roots method was used for fusarium resistance tests and inoculation procedure with a mycelium disk fixed on a small wound on the plant stem for base rot. The interspecific hybridizations provided 49 potentially hybrid genotypes. Confirmation of hybridization by microsatellite marker was verified for 57% of the analyzed genotypes. The hybrids 115-1, 115-3, 115-4, 115-5, 115-6, 115-7, 115-9 and 128 are indicated as promising genotypes for a new stage of the breeding program. In the resistance evaluation of 13 F1 hybrids to F. oxysporum f. sp. passiflorae, the genotypes 142 and 143-2 were selected as the most resistant to continue the breeding program.

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Application of Microsatellite Markers to Fingerprint and Determine the Representational Diversity within a Recently Established Elite Maize Inbred Line Breeding Program

Journal of Agricultural Science ◽

10.5539/jas.v4n6p258 ◽

2012 ◽

Vol 4 (6) ◽

Author(s):

Truong Van Nguyen ◽

Thao T. B. Doan ◽

Audrey E. Leo ◽

Cuong Manh Bui ◽

Paul W. J. Taylor ◽

...

Keyword(s):

Microsatellite Markers ◽

Inbred Line ◽

Breeding Program ◽

Maize Inbred Line ◽

Line Breeding

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Estimation of soybean genetic progress in the South of Brazil using multi-environmental yield trials

Scientia Agricola ◽

10.1590/s0103-90162009000300005 ◽

2009 ◽

Vol 66 (3) ◽

pp. 309-316 ◽

Cited By ~ 10

Author(s):

Cláudia Erna Lange ◽

Luiz Carlos Federizzi

Keyword(s):

Cropping Systems ◽

Breeding Program ◽

Maturity Group ◽

Genetic Progress ◽

Data Set ◽

Early Maturity ◽

Breeding Programs ◽

Genetic Breeding ◽

Genetic Gains ◽

Maturity Groups

The success of a genetic breeding program in a certain period can be assessed by the genetic gain observed. Genetic progress can be estimated from the multi environmental trials (MET) data which are routinely carried out by annual species breeding programs for the assessment of new commercial cultivars. A data set of 20 years of MET of advanced soybean lines derived from four breeding programs was used to estimate and to compare the genetic gains obtained for three soybean maturity groups (early, medium and late) in four cropping regions of the State of Rio Grande do Sul. The estimated yield gains ranged from 0.0 to 71.5 kg ha-1 year-1 (3.49% per year), depending on the maturity group and region, which suggests that the genetic breeding effort does not have a similar effect among the maturity groups or benefit the regions equally. There was no evidence of genetic progress for the early maturity group in any of the four regions, whereas gains in Regions I and IV were comparatively greater than those in Regions II and III. The objectives of the soybean breeding program in the region should be redirected. Since not all the experimental lines used to estimate genetic gains were commercially released, the reported genetics gains were achieved by the breeding programs rather than those achieved by the cropping systems.

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Description of Valuable Genotypes from Germplasm Collection of Hot Peppers Set by Directions of Use

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture ◽

10.15835/buasvmcn-hort:2020.0043 ◽

2020 ◽

Vol 77 (2) ◽

pp. 117

Author(s):

Ovidia Loredana AGAPIE ◽

Florin STĂNICĂ ◽

Costel VÎNĂTORU ◽

Bianca TĂNASE ◽

Elena BARCANU ◽

...

Keyword(s):

Research And Development ◽

Genetic Stability ◽

Germplasm Collection ◽

Point Of View ◽

Breeding Program ◽

Hot Pepper ◽

Genetic Breeding ◽

Hot Peppers ◽

Chili Powder

The Genetic, Breeding and Biodiversity Laboratory from Vegetable Research and Development Station Buzau (VRDS Buzău) has a valuable germplasm collection of hot pepper consisting of 200 genotypes structured by their genetic stability. In this study, seven genotypes were characterized from an agro-morphological point of view. The aim of this study was to choose the most valuable genotypes for directions of use as: fresh consumption, chili powder or for pharmaceutical use. The agro-morphological results will be used in the breeding program to obtain new genotypes adapted to the pedoclimatic conditions of Romania.

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Trait selection using procrustes analysis for the study of genetic diversity in Conilon coffee

Acta Scientiarum Agronomy ◽

10.4025/actasciagron.v42i1.43195 ◽

2020 ◽

Vol 42 ◽

pp. e43195

Author(s):

Daiana Salles Pontes ◽

Renato Domiciano Silva Rosado ◽

Cosme Damião Cruz ◽

Moysés Nascimento ◽

Ana Maria Cruz Oliveira ◽

...

Keyword(s):

Genetic Diversity ◽

Decision Making ◽

Agronomic Traits ◽

Coffea Canephora ◽

Breeding Program ◽

Procrustes Analysis ◽

Genetic Breeding ◽

Graphical Visualization ◽

Diversity Studies

Trait selection is occasionally necessary to save money and time, as well as accelerate breeding program processes. This study aimed to propose two criteria to select traits based on a Procrustes analysis that are poorly explored in genetic breeding: Criterion 1 (backward algorithm) and Criterion 2 (exhaustive algorithm). Then, these two criteria were further compared with Jolliffe’s criterion, which has often been used to select traits in genetic diversity studies. Sixteen agronomic traits were considered, and 40 Conilon coffee (Coffea canephora) accessions were evaluated. This study showed that the flexibility in selecting traits by researcher preference, graphical visualization, and Procrustes statistic through criteria 1 and 2 is a fast and reliable alternative for decision-making. These decisions are based on the removal and addition of traits for phenotyping in studies of Conilon coffee diversity that can be applied to other crops. Other relevant aspects of selection traits criteria were also discussed.

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ANALISIS KERAGAMAN GENETIK AKSESI KEDELAI INTRODUKSI DARI WILAYAH SUBTROPIS BERBASIS MORFOLOGI DAN MOLEKULER

BERITA BIOLOGI ◽

10.14203/beritabiologi.v19i3b.3894 ◽

2020 ◽

Vol 19 (3B) ◽

Author(s):

Rerenstradika Tizar Terryana ◽

Nickita Dewi Safina ◽

Suryani Suryani ◽

Kristianto Nugroho ◽

Puji Lestari

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Polymorphic Information Content ◽

Molecular Genetic ◽

Flower Color ◽

Morphological Characters ◽

Breeding Program ◽

United States Department ◽

Average Value ◽

Molecular Genetic Diversity

Genetic diversity information on soybean germplasm will establish the success of soybean breeding program. In the present study, four qualitative morphological traits information collected from Germplasm Resources Information Network (GRIN), United States Department of Agriculture (USDA) database (www.ars-grin.gov) and 10 microsatellite markers were used to analyze the relationship among 45 accessions of subtropical introduced soybean. The morphological characters of introduced soybean accessions contributed to support the result of molecular characterization. The introduced soybean accessions used in this study were diverse based on morphological and molecular characters. Based on principle component analysis, the flower color, pod color, and growth habit contributed most of the total genetic diversity. All introduced accessions were overlap into four quadrants based on principal coordinate analysis. All microsatellite primers showed polymorphism on total accession observed. High allele variation (9–27 alleles) was observed among tested accessions, with an average allele number and Polymorphic Information Content (PIC) value of 20.7 and 0.95 (0.92–0.97), respectively. All microsatellite markers showed PIC value >0.7 indicating that these markers were suitable for soybean diversity studies with high differentiation and with the average value of genetic diversity of 0.95. The phylogenetic analysis revealed that 45 soybean accessions could be divided into two major groups. Soybean accessions belonging to the same area did not always occupy the same group. The results confirmed that both morphology and molecular genetic diversity in a combined way could efficiently evaluate the variation present in different soybean accessions in any breeding program.

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