Enzymatic hydrolysis of salmon oil by native lipases: optimization of process parameters

The aim of this work is to study the acid and enzymatic hydrolysis of cocoyam peels using HCl, H2S04 acids and cellulase enzyme. The cellulase was secreted from Aspergillus Niger (A. niger) fungi. The proximate analysis of the substrate showed that cocoyam peel is a lignocellulosic biomass with a cellulose composition of 48%. The effect of the process parameters (time, temperature, acid concentration and pH) on the yield of glucose in acid and enzymatic hydrolysis of the cocoyam peel was respectively investigated. Maximum glucose yield of 44.5% was obtained after 3 days of enzymatic hydrolysis at 30°C and pH 5. The HCl acid hydrolysis showed a maximum glucose yield of 27.3% at 70°C, 5% HCl after 180 minutes. The glucose yield in H2S04 hydrolysis was relatively lower than that of the HCl with a maximum yield of 26.5% at 70°C, 5% H2SO4 after 180 minutes. In addition to, the functional groups present in the glucose synthesized from cocoyam ground peels and the standard glucose were evaluated using Fourier Transformed Infrared (FTIR). The FTIR results showed similarities in the functional groups present in both sugars. Cocoyam peel can be used for the production of glucose and further fermentative process to produce ethanol.

Download Full-text

Enzymatic Hydrolysis of Catfish (Pangasius hypophthalmus) By-Product: Kinetic Analysis of Key Process Parameters and Characteristics of the Hydrolysates Obtained

Journal of Aquatic Food Product Technology ◽

10.1080/10498850.2017.1376027 ◽

2017 ◽

Vol 26 (9) ◽

pp. 1070-1082

Author(s):

Nguyen Cong Ha ◽

Dang Minh Hien ◽

Nguyen Thi Thuy ◽

Loc Thai Nguyen ◽

Lavaraj Devkota

Keyword(s):

Enzymatic Hydrolysis ◽

Kinetic Analysis ◽

Process Parameters ◽

Hydrolysis Of

Download Full-text

Study of the Effect of Process Parameters on the Yield of Fermentable Sugar from Tuber Peels Via Acid and Enzyme Hydrolysis

Journal of Energy Research and Reviews ◽

10.9734/jenrr/2021/v9i230228 ◽

2021 ◽

pp. 24-32

Author(s):

Onoh Ikechukwu Maxwell ◽

Anho Lawrence Oghenerivwe ◽

Egwuagu Onyekachi

Keyword(s):

Enzymatic Hydrolysis ◽

Functional Groups ◽

Process Parameters ◽

Maximum Yield ◽

Proximate Analysis ◽

Enzyme Hydrolysis ◽

Glucose Yield ◽

Hydrolysis Of ◽

Fermentative Process ◽

Water Yam

The aim of this work is to study the acid and enzymatic hydrolysis of water yam peels using HCl, H2S04 acids and cellulase enzyme. The cellulase was secreted from Aspergillus niger (A.niger). The proximate analysis of the substrate showed that water yam peel is a lignocellulosic biomass with a cellulose composition of 48%. The effect of the process parameters (time, temperature, acid concentration and pH) on the yield of glucose in acid and enzymatic hydrolysis of the water yam peel was respectively investigated. Maximum glucose yield of 44.5% was obtained after 3 days of enzymatic hydrolysis at 30°C and pH 5. The HCl acid hydrolysis showed a maximum glucose yield of 27.3% at 70°C, 5% HCl after 180 minutes. The glucose yield in H2S04 hydrolysis was relatively lower than that of the HCl with a maximum yield of 26.5% at 70°C, 5% H2SO4 after 180 minutes. In addition to, the functional groups present in the glucose synthesized from ground water yam peels and the standard glucose were evaluated using Fourier Transformed Infrared (FTIR) Spectroscopy. The FTIR results showed similarities in the functional groups present in both sugars. Yam peel can be used for the production of glucose and further fermentative process to produce ethanol.

Download Full-text

Enzymatic Hydrolysis of Blend of Lignicellulosic Materials for Reducing Sugar Production: Screening of Significant Process Factors

Asian Journal of Chemical Sciences ◽

10.9734/ajocs/2020/v8i419048 ◽

2020 ◽

pp. 1-10

Author(s):

Bathlomew Chukwudi Amadi ◽

Philomena Kañulia Igbokwe ◽

Chijioke Elijah Onu

Keyword(s):

Enzymatic Hydrolysis ◽

Process Parameters ◽

Confidence Level ◽

Statistical Significance ◽

Latin Square ◽

Reducing Sugar ◽

Sugar Yield ◽

Blend Ratio ◽

Process Factors ◽

Hydrolysis Of

The screening of the process parameters on the enzymatic hydrolysis of a blend of two lignocellulosic materials - corn cob (CC) and deseeded fluted pumpkin fruit (DFPF) using Trichoderma reesei was the focus of the present study. Four process parameters – time, temperature, substrate blend ratio and pH were screened for statistical significance using a 4 x 4 matrix of Graeco-Latin square design of experiment. The reducing sugar yield was determined using the dinitrosalisylic acid (DNS) method and maximum reducing sugar yield of 57.92mg/ml obtained in 2days at 40oC, blend ratio of 0.2:0.8 (CC:DFPF) and pH 5.5. Result of the Analysis of Variance (ANOVA) obtained showed that with 3 degrees of freedom and 6 residual degree of freedom at 95% confidence level (i.e. 0.05 significance), time, temperature and substrate blend ratio significantly affected the enzymatic hydrolysis process whereas the effect of pH was not significant. However, only time was significant at 99% confidence level (0.01 significance) while temperature, substrate blend ratio and pH were not significant. This study has highlighted the significant factors among the process variables in emzymatic hydrolysis of a blend of CC and DFPF.

Download Full-text