Enzymatic Hydrolysis of Blend of Lignicellulosic Materials for Reducing Sugar Production: Screening of Significant Process Factors

The screening of the process parameters on the enzymatic hydrolysis of a blend of two lignocellulosic materials - corn cob (CC) and deseeded fluted pumpkin fruit (DFPF) using Trichoderma reesei was the focus of the present study. Four process parameters – time, temperature, substrate blend ratio and pH were screened for statistical significance using a 4 x 4 matrix of Graeco-Latin square design of experiment. The reducing sugar yield was determined using the dinitrosalisylic acid (DNS) method and maximum reducing sugar yield of 57.92mg/ml obtained in 2days at 40oC, blend ratio of 0.2:0.8 (CC:DFPF) and pH 5.5. Result of the Analysis of Variance (ANOVA) obtained showed that with 3 degrees of freedom and 6 residual degree of freedom at 95% confidence level (i.e. 0.05 significance), time, temperature and substrate blend ratio significantly affected the enzymatic hydrolysis process whereas the effect of pH was not significant. However, only time was significant at 99% confidence level (0.01 significance) while temperature, substrate blend ratio and pH were not significant. This study has highlighted the significant factors among the process variables in emzymatic hydrolysis of a blend of CC and DFPF.

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High-temperature heating and microwave pretreatments: A new light in bamboo's enzymatic hydrolysis

Thermal Science ◽

10.2298/tsci1603999f ◽

2016 ◽

Vol 20 (3) ◽

pp. 999-1002 ◽

Cited By ~ 1

Author(s):

Jiajia Fu ◽

Yu Li ◽

Qun Zhang ◽

Shen Shen ◽

Xiao-Ying Du ◽

...

Keyword(s):

High Temperature ◽

Enzymatic Hydrolysis ◽

Protein Adsorption ◽

Reducing Sugar ◽

Sugar Yield ◽

X Ray ◽

Temperature Heating ◽

Hydrolysis Of ◽

High Temperature Heating

A combined technology of high-temperature heating and microwave is proposed to treat bamboo substrates prior to enzymatic hydrolysis to overcome the limits of ei- ther technology. The combined technology helps to promote the reducing sugar yield from enzymatic hydrolysis of bamboo. The protein adsorption test indicated that more enzyme proteins could adsorb on the surface of bamboo pretreated by the combined technology. Thus, the possibility of enzymes to hydrolyze bamboo increased. Meanwhile, X-ray diffractometry tested revealed that the combined technology benefited the removal of non-cellulosic substances from bamboo and resulted in a higher crystallinity.

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Effect of Severity Factor on the Subcritical Water and Enzymatic Hydrolysis of Coconut Husk for Reducing Sugar Production

BULLETIN OF CHEMICAL REACTION ENGINEERING AND CATALYSIS ◽

10.9767/bcrec.15.3.8870.786-797 ◽

2020 ◽

Vol 15 (3) ◽

pp. 786-797

Author(s):

Maktum Muharja ◽

Nur Fadhilah ◽

Rizki Fitria Darmayanti ◽

Hanny Frans Sangian ◽

Tantular Nurtono ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Subcritical Water ◽

Biomass Conversion ◽

Reducing Sugar ◽

Sugar Yield ◽

Sugar Production ◽

Severity Factor ◽

Coconut Husk ◽

Hydrolysis Process ◽

Hydrolysis Of

Preventing the further degradation of monomeric or oligomeric sugar into by-product during biomass conversion is one of the challenges for fermentable sugar production. In this study, the performance of subcritical water (SCW) and enzymatic hydrolysis of coconut husk toward reducing sugar production was investigated using a severity factor (SF) approach. Furthermore, the optimal condition of SCW was optimized using response surface methodology (RSM), where the composition changes of lignocellulose and sugar yield as responses. From the results, at low SF of SCW, sugar yield escalated as increasing SF value. In the enzymatic hydrolysis process, the effect of SCW pressure is a significant factor enhancing sugar yield. A maximum total sugar yield was attained on the mild SF condition of 2.86. From this work, it was known that the SF approach is sufficient parameter to evaluate the SCW and enzymatic hydrolysis of coconut husk. Copyright © 2020 BCREC Group. All rights reserved

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The influence of solid/liquid separation techniques on the sugar yield in two-step dilute acid hydrolysis of softwood followed by enzymatic hydrolysis

Biotechnology for Biofuels ◽

10.1186/1754-6834-2-6 ◽

2009 ◽

Vol 2 (1) ◽

pp. 6 ◽

Cited By ~ 29

Author(s):

Sanam Monavari ◽

Mats Galbe ◽

Guido Zacchi

Keyword(s):

Enzymatic Hydrolysis ◽

Acid Hydrolysis ◽

Sugar Yield ◽

Dilute Acid ◽

Dilute Acid Hydrolysis ◽

Liquid Separation ◽

Separation Techniques ◽

Solid Liquid ◽

Solid Liquid Separation ◽

Hydrolysis Of

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Optimization of Fermentable Sugar Production from Pineapple Leaf Waste (Ananas comosus [L.] Merr) by Enzymatic Hydrolysis

Jurnal Presipitasi Media Komunikasi dan Pengembangan Teknik Lingkungan ◽

10.14710/presipitasi.v18i1.73-80 ◽

2021 ◽

Vol 18 (1) ◽

pp. 73-80

Author(s):

Yohanita Restu Widihastuty ◽

Sutini Sutini ◽

Aida Nur Ramadhani

Keyword(s):

Enzymatic Hydrolysis ◽

Complex Structure ◽

Reducing Sugar ◽

Sugar Yield ◽

Ananas Comosus ◽

Optimum Operating ◽

Cellulose Content ◽

Optimum Operating Condition ◽

Operating Condition ◽

Cellulase Enzyme

Pineapple leaf waste is one agricultural waste that has high cellulose content. Pineapple leaf waste's complex structure contains a bundle of packed fiber that makes it hard to remove lignin and hemicellulose structure, so challenging to produce reducing sugar. Dried pineapple leaf waste pretreated with a grinder to break its complex structure. Delignification process using 2% w/v NaOH solution at 87oC for 60 minutes has been carried out to remove lignin and hemicellulose structure so reducing sugar could be produced. Delignified pineapple leaf waste has been enzymatic hydrolyzed using cellulase enzyme (6 mL, 7 mL, and 8 mL) to produce reducing sugar. The sample was incubated in an incubator shaker at 155 rpm at 45, 55, and 60oC for 72 hours. Determination of reducing sugar yield had been carried out using the Dubois method and HPLC. The model indicated that the optimum operating condition of enzymatic hydrolysis is 7 mL of cellulase enzyme at 55oC to produce 96,673 mg/L reducing sugar. This result indicated that the enzymatic hydrolysis operating condition improved the reducing sugar yield from pineapple leaf waste. The optimum reducing sugar yield can produce biofuel by the saccharification process.

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Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya

E-Journal Menara Perkebunan ◽

10.22302/ppbbi.jur.mp.v83i1.12 ◽

2016 ◽

Vol 83 (1) ◽

Author(s):

Vera BARLIANTI ◽

Deliana DAHNUM ◽

. MURYANTO ◽

Eka TRIWAHYUNI ◽

Yosi ARISTIAWAN ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Oil Palm ◽

Volume Ratio ◽

Enzyme Concentration ◽

Reducing Sugar ◽

Economic Feasibility ◽

First Order ◽

Empty Fruit Bunches ◽

Hydrolysis Process ◽

Hydrolysis Of

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi. Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung. Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu. Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The maximum sugar concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.

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Reducing sugar loss in enzymatic hydrolysis of ethylenediamine pretreated corn stover

Bioresource Technology ◽

10.1016/j.biortech.2016.11.031 ◽

2017 ◽

Vol 224 ◽

pp. 405-410 ◽

Cited By ~ 8

Author(s):

Wen-Chao Li ◽

Xia Li ◽

Lei Qin ◽

Jia-Qing Zhu ◽

Xiao Han ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Corn Stover ◽

Reducing Sugar ◽

Pretreated Corn Stover ◽

Hydrolysis Of ◽

Sugar Loss

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Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

Journal of Energy ◽

10.1155/2016/8506214 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 20

Author(s):

Robinson Timung ◽

Narendra Naik Deshavath ◽

Vaibhav V. Goud ◽

Venkata V. Dasu

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Crystallinity Index ◽

Sem Analysis ◽

Reducing Sugar ◽

Dilute Acid Pretreatment ◽

Crystalline Cellulose ◽

Dilute Acid ◽

Acid Pretreatment ◽

Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

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Synergistic effects on process parameters to enhance enzymatic hydrolysis of alkaline oil palm fronds

Industrial Crops and Products ◽

10.1016/j.indcrop.2018.06.037 ◽

2018 ◽

Vol 122 ◽

pp. 617-626 ◽

Cited By ~ 3

Author(s):

Masniroszaime Md Zain ◽

Abdul Wahab Mohammad ◽

Shuhaida Harun ◽

Nurul Aina Fauzi ◽

Nur Hanis Hayati Hairom

Keyword(s):

Enzymatic Hydrolysis ◽

Process Parameters ◽

Oil Palm ◽

Synergistic Effects ◽

Oil Palm Fronds ◽

Palm Fronds ◽

Hydrolysis Of

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Evaluation of the Interactive Effect Pretreatment Parameters via Three Types of Microwave-Assisted Pretreatment and Enzymatic Hydrolysis on Sugar Yield

Processes ◽

10.3390/pr8070787 ◽

2020 ◽

Vol 8 (7) ◽

pp. 787 ◽

Cited By ~ 1

Author(s):

Saleem Ethaib ◽

Rozita Omar ◽

Mustapa Kamal Siti Mazlina ◽

Awang Biak Dayang Radiah

Keyword(s):

Response Surface Methodology ◽

Enzymatic Hydrolysis ◽

Interactive Effect ◽

Reducing Sugar ◽

Sugar Yield ◽

Solid Loading ◽

Acid Pretreatment ◽

Glucose Yield ◽

Sago Palm ◽

Microwave Assisted

This study aims to evaluate the sugar yield from enzymatic hydrolysis and the interactive effect pretreatment parameters of microwave-assisted pretreatment on glucose and xylose. Three types of microwave-assisted pretreatments of sago palm bark (SPB) were conducted for enzymatic hydrolysis, namely: microwave-sulphuric acid pretreatment (MSA), microwave-sodium hydroxide pretreatment (MSH), and microwave-sodium bicarbonate (MSB). The experimental design was done using a response surface methodology (RSM) and Box–Behenken Design (BBD). The pretreatment parameters ranged from 5–15% solid loading (SL), 5–15 min of exposure time (ET), and 80–800 W of microwave power (MP). The results indicated that the maximum total reducing sugar was 386 mg/g, obtained by MSA pretreatment. The results also illustrated that the higher glucose yield, 44.3 mg/g, was found using MSH pretreatment, while the higher xylose yield, 43.1 mg/g, resulted from MSA pretreatment. The pretreatment parameters MP, ET, and SL showed different patterns of influence on glucose and xylose yield via enzymatic hydrolysis for MSA, MSH, and MSB pretreatments. The analyses of the interactive effect of the pretreatment parameters MP, ET, and SL on the glucose yield from SPB showed that it increased with the high MP and longer ET, but this was limited by low SL values. However, the analysis of the interactive effect of the pretreatment parameters on xylose yields revealed that MP had the most influence on the xylose yield for MSA, MSH, and MSB pretreatments.

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