Evaluation of methanolic crude extract of Linum usitatissimum for the removal of biofilm in diabetic foot isolates

Linum usitatissimum L is a widely used traditionally for multiple ailments. The present research was carried out to explore the antimicrobial, and anti-biofilm activity of crude extract of Linum usitatissimum L (Lu. Cr). Phytochemical and proximate analyses were performed. The bandages of diabetic foot patients were collected from the various hospitals. The bandages were cultured to isolate the bacterial strains present on it. The disc diffusion method was used to identify the antimicrobial potential whereas the minimum inhibitory concentration of the Lu.Cr were also determined. Proximate analysis confirms moisture content 8.33%, ash content 4.33%, crude protein 21.20%, crude fat 49.2% and crude fiber 5.63%. It was revealed that Gram-positive bacteria are most prevalent among all study groups. Lu.Cr possess significant bactericidal potential against S. aureus among all other microbes. Owing to this potential, linseed coated bandages can be used alternatively for the treatment of diabetic foot.

Influence of Post- and Pre-Acid Treatment during Hydrothermal Carbonization of Sewage Sludge on P-Transformation and the Characteristics of Hydrochar

Phosphorus (P) recovery from alternative P-rich residues is essential to meet the growing demands of food production globally. Despite sewage sludge being a potential source for P, its direct application on agricultural land is controversial because of the obvious concerns related to heavy metals and organic pollutants. Further, most of the available P recovery and sludge management technologies are cost-intensive as they require mandatory dewatering of sewage sludge. In this regard, hydrothermal carbonization (HTC) has gained great attention as a promising process to effectively treat the wet sewage sludge without it having to be dewatered, and it simultaneously enables the recovery of P. This study was conducted to analyse and compare the influence of acid (H2SO4) addition during and after HTC of sewage sludge on P leaching and the characteristics of hydrochar. The obtained results suggested that despite using the same amount of H2SO4, P leaching from solid to liquid phase was significantly higher when acid was used after the HTC of sewage sludge in comparison with acid utilization during the HTC process. After HTC, the reduction in acid-buffering capacity of sewage sludge and increase in solubility of phosphate precipitating metal ions had a greater influence on the mobilization of P from solid to liquid phase. In contrast, utilization of H2SO4 in different process conditions did not have a great influence on proximate analysis results and calorific value of consequently produced hydrochar.

Physico-chemical and Sensory Evaluation of Biscuits Developed from Blends of Jowar (Sorghum), Organic Jaggery and Whole Wheat Flours

Background: Jowar (Sorghum) is one of the most popular millet grown in tropical and semi-arid regions. The present study was aimed to design and develop the process technology for the preparation of biscuits from blends of jowar and jaggery by replacing wheat flour. Methods: The biscuits were analysed for physico-chemical and sensory properties. The proportions taken were 30:70:60, 40:60:60, 50:50:60, 60:40:60, 70:30:60 and 100:0:60 of jowar flour, whole wheat flour and jaggery respectively. Sensory evaluation was performed by using composite scoring test and means were evaluated by Krushkal Walli H-Test. Result: Sensory evaluation revealed that the ratio of 100:0:60 (SWJ 6) jowar, whole wheat flour and jaggery has highest acceptability and was considered for further studies. The physical parameters of biscuits such as mass (20 g), thickness (7 mm), spread factor (64.28), diameter (45 mm) and color values were determined. The texture analysis revealed that the biscuits held hardness (32.5 g), fracturability (8.60 mm) and cutting strength (3.390 kg). Proximate analysis was estimated as energy (583 kcal), carbohydrate (92.6 g), protein (10.8 g), fat (4.8 g), crude fibre (8.6 g), calcium (46 mg), zinc (58.3 mg), iron (16.1 mg) and potassium (420 mg) per 100 g for SWJ 6 proportion.

Potential Bio-Fuel from Refinery Waste through Anaerobic Digestion

In this study we report biofuel potential in waste cake obtained from oil refinery. The sample was analyzed for its calorific value using auto bomb calorimeter (LECO AC-500), proximate analysis using Thermogravimetric analyzer (LECO 701) and elemental analysis using CHNS analyzer (LECO Tru-Spec). The elemental analysis of dry waste cake vs wet cake depicted the percentage composition of carbon (49.8%, 40.8%), hydrogen (7.9%, 6.0%), nitrogen (2.8%, 1.9%), Sulphur (1.9%, 0.5%) and oxygen content (37.6%, 40.4%). As for as the thermal degradation behavior of dry and wet cake in TGA is concerned, higher moisture contents (68.50%) found in wet cake and lower in dry cake (40.1%). Whereas the volatile matter in dry cake (30.9%) and low volatile in wet cake (14.3%). Similarly, %age of ash become high in dry cake (17.3%) and low in wet cake (5.11%). The results reflected that higher heating value of dry waste cake is higher (22.5 MJ/kg) than wet waste cake (20.5 MJ/kg) and commonly used sugarcane bagasse (17.88 MJ/kg).

KARAKTERISTIK ORGANOLEPTIK DAN PROKSIMAT MIE BASAH DENGAN PENAMBAHAN TEPUNG TERIPANG PASIR (Holothuria scabra)

ABSTRACTThe purpose of this study was to determine the effect of the addition of sea cucumber flour on the organoleptic and proximate characteristics of wet noodles. This study consisted of four treatments, namely without the addition of sand sea cucumber flour (T0), the addition of 5% sea cucumber flour (T1), the addition of 10% sea cucumber flour (T2), and the addition of 15% sea cucumber flour (T3). The analyzed parameters were organoleptic and physicochemical values. This study used a completely randomized design (CRD). Data were analyzed using Analysis of Variance (ANOVA) and continued with Duncan's Multiple Range Test (DMRT) at a 95% confidence level. The results show that the effect of adding sand sea cucumber flour had a very significant effect on organoleptic assessment in T1 treatment (95% wheat flour: 5% sea cucumber flour) with preference scores of color, aroma, taste, and texture reached 4.03 (like), 4.06 (like), 3.91 (like), and 4.06 (like). Proximate analysis of the best treatment shows that it contained 50.12% water, 1.18% ash, 0.20% fat, 7.23% protein, and 41.75% carbohydrate. The results show that the product met the standard of SNI 01-2987-1992 for ash, fat, and protein contents. Keywords: Sea Cucumber, Wet NoodlesABSTRAKTujuan penelitian ini untuk menentukan pengaruh penambahan tepung teripang pasir terhadap karakteristik organoleptik dan proksimat mie basah. Penelitian ini terdiri dari empat perlakuan yaitu tanpa penambahan tepung teripang pasir (T0), penambahan tepung teripang pasir 5% (T1), penambahan tepung teripang pasir 10% (T2), dan penambahan tepung teripang pasir 15% (T3), lalu dianalisis terhadap nilai organoleptik dan fisikokimia. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Data di analisis menggunakan Analysis of Varian (ANOVA) dan dilanjutkan dengan uji Duncan’s Multiple Range Test (DMRT) pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa pengaruh penambahan tepung teripang pasir berpengaruh sangat nyata terhadap penilaian organoleptik pada perlakuan T1 (tepung terigu 95% : tepung teripang pasir 5%) sebesar warna 4.03 (suka), aroma 4.06 (suka), rasa 3.91 (suka), dan tekstur 4.06 (suka). Analisis proksimat perlakuan terbaik diperoleh nilai kadar air 50.12%, kadar abu 1.18%, kadar lemak 0.20%, kadar protein 7.23% dan kadar karbohidrat 41.75%. Berdasarkan hasil penelitian nilai kadar abu, kadar lemak, dan kadar protein telah memenuhi SNI 01-2987-1992.Kata kunci: Teripang Pasir, Mie Basah

Evaluation of nutritive values and digestibility’s cacao( Theobroma cacao L.) pod husk fermented with lingzhi mushroom (Ganoderma lucidum) at different concentration and incubation time

Cacao pod husk has been widely utilized as animal feed. The purpose of this study is to evaluate nutritive values and digestibility of fermented cacao pod husk with different concentration and incubation time. A completely randomized factorial design consisting two factors; lingzhi mushroom concentration (K1 = 7,5%; K2 = 15%) and incubation time (L1 = 15 d; L2 =30 d; L3 = 45 d) was employed in this study (n=3 replicates). Proximate analysis was performed to determine nutritive values of fermented cacao pod husk. Fermented samples were then subjected to an in vitro digestibility with rumen fluid and McDougall’s buffer mixture. The results of study reveal that cacao pod husk fermented with the concentration of 15% at 15d and 30 d significantly increased (P<0.05) crude protein content but not for other parameters. Dry and organic matter digestibility of cacao pod husk fermented with 7.5% of lingzhi mushroom at 45 d significantly improved (P<0.05) in vitro dry and organic matter of treatment. In conclusion, cacao pod husk fermented with different concentration and incubation time was able to improve the nutritive values and in vitro digestibility of cacao pod husk.

Dietary Inclusion of Citrullus lanatus Seed in Fish Feed: Proximate Analysis, Growth Performance and Feed Utilization in Clarias gariepinus Fingerlings

One of the factors militating against fish farming in Nigeria have been lack of adequate feed that are formulated to meet the nutrient requirement of culturable fish species. To this end, the study on dietary inclusion of Citrullus lanatus (watermelon) seed in fish feed: the proximate analysis, determination of growth performance and feed utilization in Clarias gariepinus fingerlings was carried out between August and October 2019. The proximate analysis and economic viability were determined according to standard methods. The experimental diet was set up across varying proportions: 0% as the control, 15%, 20%, 25% and 36% inclusion levels respectively. The result of the proximate analysis for the experimental diets showed that diet 3 (20.00% inclusion) had the highest crude protein content; lipid level was highest in diet 4 (25% inclusion); while diet 1 (control) had the highest dry matter, ash and nitrogen free extract contents. The result of the experiment showed that 36% watermelon seed inclusion yielded the highest growth performance and feed utilization in Clarias gariepinus fingerlings. However, there was no significant difference (P > 0.05) in relation to inclusion percentages of watermelon seed on growth performance in Clarias gariepinus fingerlings. In conclusion, relatively high proportion of watermelon seed inclusion in fish feed formulation is hereby recommended in order to improve growth performance and feed utilization in Clarias gariepinus fingerlings.

Effect of Incorporation of Oats and Malted Barley Flours on the Quality of Cookies

The study aimed to determine the potential of oats and malted barley flours incorporation on cookie making. D-optimal mixture design was employed for formulating the recipe of cookies. Seven different formulations of recipe containing oats flour: malted barley flour: wheat flour in the ratio of 20:25:55, 15:30:55, 13:32:55, 10:35:55, 7:38:55, 5:40:55 and 100:0:0 was prepared. The prepared cookies were subjected to sensory evaluation for consumer acceptability and the data obtained were statistically analyzed using two-way ANOVA (no blocking) at a 5% level of significance. From the mean sensory scores, the formulation 10:35:55 was selected as the optimized formulation and subjected to proximate analysis. The optimized cookies were significantly (p<0.05) superior in the case of protein (7.50%), fat (22.93%), fiber (2.90%), and ash content (0.97%) to wheat cookies (6.43%, 18.83% 0.83% and 0.41% respectively) as seen higher in oats and malted barley flours than wheat flour. The calcium content was found to be significantly (p<0.05) higher in optimized cookies (35.3 mg/100g) than wheat cookies (18.40 mg/).