scholarly journals Study of the Effect of Process Parameters on the Yield of Fermentable Sugar from Tuber Peels Via Acid and Enzyme Hydrolysis

2021 ◽  
pp. 24-32
Author(s):  
Onoh Ikechukwu Maxwell ◽  
Anho Lawrence Oghenerivwe ◽  
Egwuagu Onyekachi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Functional Groups ◽  
Process Parameters ◽  
Maximum Yield ◽  
Proximate Analysis ◽  
Enzyme Hydrolysis ◽  
Glucose Yield ◽  
Hydrolysis Of ◽  
Fermentative Process ◽  
Water Yam

The aim of this work is to study the acid and enzymatic hydrolysis of water yam peels using HCl, H2S04 acids and cellulase enzyme. The cellulase was secreted from Aspergillus niger (A.niger). The proximate analysis of the substrate showed that water yam peel is a lignocellulosic biomass with a cellulose composition of 48%. The effect of the process parameters (time, temperature, acid concentration and pH) on the yield of glucose in acid and enzymatic hydrolysis of the water yam peel was respectively investigated. Maximum glucose yield of 44.5% was obtained after 3 days of enzymatic hydrolysis at 30°C and pH 5. The HCl acid hydrolysis showed a maximum glucose yield of 27.3% at 70°C, 5% HCl after 180 minutes. The glucose yield in H2S04 hydrolysis was relatively lower than that of the HCl with a maximum yield of 26.5% at 70°C, 5% H2SO4 after 180 minutes. In addition to, the functional groups present in the glucose synthesized from ground water yam peels and the standard glucose were evaluated using Fourier Transformed Infrared (FTIR) Spectroscopy. The FTIR results showed similarities in the functional groups present in both sugars. Yam peel can be used for the production of glucose and further fermentative process to produce ethanol.

scholarly journals Study of the effect of process parameters on the yield of fermentable sugar from red cocoyam (Xanthosoma sagittifolium) peels via acid and enzyme hydrolysis

2021 ◽  
Vol 1 (1) ◽  
pp. 061-069
Author(s):  
Onoh Ikechukwu Maxwell ◽  
Anho Lawrence Oghenerivwe ◽  
Egwuagu Onyekachi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Functional Groups ◽  
Process Parameters ◽  
Maximum Yield ◽  
Proximate Analysis ◽  
Enzyme Hydrolysis ◽  
Glucose Yield ◽  
Xanthosoma Sagittifolium ◽  
Hydrolysis Of ◽  
Fermentative Process

The aim of this work is to study the acid and enzymatic hydrolysis of cocoyam peels using HCl, H2S04 acids and cellulase enzyme. The cellulase was secreted from Aspergillus Niger (A. niger) fungi. The proximate analysis of the substrate showed that cocoyam peel is a lignocellulosic biomass with a cellulose composition of 48%. The effect of the process parameters (time, temperature, acid concentration and pH) on the yield of glucose in acid and enzymatic hydrolysis of the cocoyam peel was respectively investigated. Maximum glucose yield of 44.5% was obtained after 3 days of enzymatic hydrolysis at 30°C and pH 5. The HCl acid hydrolysis showed a maximum glucose yield of 27.3% at 70°C, 5% HCl after 180 minutes. The glucose yield in H2S04 hydrolysis was relatively lower than that of the HCl with a maximum yield of 26.5% at 70°C, 5% H2SO4 after 180 minutes. In addition to, the functional groups present in the glucose synthesized from cocoyam ground peels and the standard glucose were evaluated using Fourier Transformed Infrared (FTIR). The FTIR results showed similarities in the functional groups present in both sugars. Cocoyam peel can be used for the production of glucose and further fermentative process to produce ethanol.

Synergistic effects on process parameters to enhance enzymatic hydrolysis of alkaline oil palm fronds

2018 ◽  
Vol 122 ◽  
pp. 617-626 ◽  
Author(s):  
Masniroszaime Md Zain ◽  
Abdul Wahab Mohammad ◽  
Shuhaida Harun ◽  
Nurul Aina Fauzi ◽  
Nur Hanis Hayati Hairom
Keyword(s):  
Enzymatic Hydrolysis ◽  
Process Parameters ◽  
Oil Palm ◽  
Synergistic Effects ◽  
Oil Palm Fronds ◽  
Palm Fronds ◽  
Hydrolysis Of

Optimization of olive pomace enzymatic hydrolysis for fermentable sugar production

2016 ◽  
Vol 46 (6) ◽  
pp. 778-790 ◽  
Author(s):  
Ghassan Abo Chameh ◽  
Fadi Kheder ◽  
Francois Karabet
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ph Value ◽  
Maximum Yield ◽  
Olive Pomace ◽  
Mixing Rate ◽  
Content Type ◽  
Hydrolysis Yield ◽  
Pretreatment Time ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

Purpose The purpose of this paper was to find out the appropriate enzymatic hydrolysis conditions of alkali pretreated olive pomace (OP) which enable maximum yield of reducing sugar. Design/methodology/approach The commercial enzymatic preparation (Viscozyme® L) was used for the hydrolysis of OP. The effects of pretreatment, time, temperature, pH, enzyme quantity and substrate loading on the hydrolysis yield were investigated. Findings This study showed that enzymatic hydrolysis of OP using Viscozyme® L can be successfully performed at 50°C. Alkaline pretreatment step of OP prior the enzymatic hydrolysis was indispensable. The hydrolysis yield of alkaline pretreated OP was 2.6 times higher than the hydrolysis yield of untreated OP. Highest hydrolysis yield (33.5 ± 1.5 per cent) was achieved after 24 h using 1 per cent (w/v) OP load in the presence of 100 μl Viscozyme® L at 50°C and pH 5.5 with mixing rate of 100 rpm (p = 0.05). Originality/value Reaction time, temperature, pH value and enzyme quantity were found to have a significant effect on enzymatic hydrolysis yield of alkali pretreated of OP. Although high-solid loadings of OP lowered the hydrolysis yield, it produced higher concentration of reducing sugars, which may render the OP conversion process more economically feasible.

scholarly journals Enzymatic hydrolysis of potato starch and ethanol production

2004 ◽  
Vol 58 (7-8) ◽  
pp. 322-326 ◽  
Author(s):  
Miodrag Lazic ◽  
Suzana Raskovic ◽  
Mihajlo Stankovic ◽  
Vlada Veljkovic
Keyword(s):  
Particle Size ◽  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Potato Starch ◽  
Enzyme Hydrolysis ◽  
Potato Flour ◽  
Yeast Saccharomyces Cerevisiae ◽  
Enzyme Preparations ◽  
Dextrose Equivalent ◽  
Hydrolysis Of

The hydrolysis of potato starch using one (Termamyl or Fungamyl) and two combined (Termamyl and Supersan) commercial enzyme preparations and ethanol production from the hydrolysates obtained using the yeast Saccharomyces cerevisiae were studied. Potato tubers were previously prepared as mash or flour. The study dealt with the effects of the hydromodulus (1:1 and 1:0.5), particle size (0.1, 0.2 and 0.4 mm) as well as the type and concentration of enzyme on the enzymatic hydrolysis of potato starch. The highest dextrose equivalent (79.3%, DE) was achieved during two-enzyme hydrolysis (combination of two enzyme preparations: Termamyl 14.4 KNU/dm3 and Supersan 28.8 AGU/dm3) of starch from potato flour (particle size: 0.1-0.2 mm) at an inital starch concentration of 75 g/dm3. During the two-enzyme hydrolysis, a lower DE (61%) was achieved when potato mash was used as a starch source. Using Termamyl (14,4 KNU/dm3) and potato mash a higher DE was achieved at the hydromodulus 1:1 (51.2%) than at the hydromodulus 1:0.5 (40.9%). The highest ethanol concentration (5.0 vol%) was obtained when the hydrolyzate of potato flour from the two-enzyme process was used as a substrate for alcoholic fermentation.

scholarly journals Chemical Pretreatment And Enzymatic Hydrolysis Of Mixed Source-Separated Organic (SSO) And Wood Waste

2021 ◽  
Author(s):  
Michael Faye
Keyword(s):  
Enzyme Hydrolysis ◽  
Hydrolysis Rate ◽  
Lime Treatment ◽  
Demolition Waste ◽  
Glucose Yield ◽  
Cellulose Solvent ◽  
Hydrolysis Yield ◽  
Lime Pretreatment ◽  
Hydrolysis Of

This paper examines the effectiveness of two pretreatments on Source-Separated Organic waste (SSO) mixed with wood wastes: long term lime for SSO mixed with forestry waste (hardwoods), and the cellulose solvent-organic lignocellulose fractionation (COSLIF) method, with SSO and demolition waste (softwoods). For long term lime treatment, the highest overall conversions from cellulose to glucose and xylose were 50.4%, and 43.5% respectively. The best temperature found for long term lime pretreatment was 65°C. The COSLIF pretreatment glucose yield was found to be 93.7%. The highest enzyme hydrolysis yield found was 93.5% for a cellulase loading of 30 FPU/g glucan at 50°C. The best hydrolysis yield found at lower loading (10 FPT/g glucan), was 83.5%. At 40 and 50°C, all peak hydrolysis yields were achieved between 12 and 24 hours. A drop in temperature below 40°C caused a slowing of the hydrolysis rate.

scholarly journals Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood

Energies ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 4552 ◽  
Author(s):  
Merve Nazli Borand ◽  
Asli Isler Kaya ◽  
Filiz Karaosmanoglu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Liquid Fraction ◽  
Sugar Content ◽  
Release Time ◽  
Variable Parameters ◽  
Glucose Yield ◽  
Steam Explosion Pretreatment ◽  
Saccharification Yield ◽  
Hydrolysis Of

Pressure, temperature, and retention time are the most studied parameters in steam explosion pretreatment. However, this work aimed to fix these parameters and to evaluate the influences of several less investigated steam explosion parameters on the saccharification yield in hydrolysis. In this study, firstly, pinewood samples smaller than 200 µm were treated with steam explosion at 190 °C for 10 min. The variable parameters were biomass loading, N2 pressure, and release time. Steam-exploded samples were hydrolyzed with the Trichoderma reesei enzyme for saccharification for 72 h. The sugar content of the resultant products was analyzed to estimate the yield of sugars (such as glucose, xylose, galactose, mannose, and arabinose). The best glucose yield in the pulp was achieved with 4 g of sample, N2 pressure of 0.44 MPa, and short release time (22 s). These conditions gave a glucose yield of 97.72% in the pulp, and the xylose, mannose, galactose, and arabinose yields in the liquid fraction were found to be 85.59%, 87.76%, 86.43%, and 90.3%, respectively.

scholarly journals Enzymatic hydrolysis of salmon oil by native lipases: optimization of process parameters

2009 ◽  
Vol 20 (1) ◽  
pp. 117-124 ◽  
Author(s):  
Patrícia de O. Carvalho ◽  
Paula R. B. Campos ◽  
Maximiliano D'Addio Noffs ◽  
Patrícia B. L. Fregolente ◽  
Leonardo V. Fregolente
Keyword(s):  
Enzymatic Hydrolysis ◽  
Process Parameters ◽  
Optimization Of Process Parameters ◽  
Hydrolysis Of

The influence of urea and formaldehyde on enzymatic hydrolysis of cellulose

2020 ◽  
Vol 110 ◽  
pp. 92-96
Author(s):  
Monika Marchwicka ◽  
Anna Lesiak ◽  
Andrzej Radomski
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Enzymatic Hydrolysis ◽  
High Performance ◽  
Glucose Yield ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Effect of selected urea and formaldehyde concentrations on glucose yield of enzymatic hydrolysis of cellulose was investigated. Urea and formaldehyde were added separately at the concentrations of 0.001, 0.002 and 0.005 g/cm3. Glucose was determined by high-performance liquid chromatography (HPLC). It was found that the used concentrations of urea didn’t influence glucose yield. In the case of formaldehyde, the results vary between used concentrations. The glucose yield of enzymatic hydrolysis of cellulose with the highest investigated concentration of formaldehyde (0.005 g/cm3) decreased by 50 %.

Sign in / Sign up

Export Citation Format

Share Document