scholarly journals Molecular characterization of van genes found in vancomycin-resistant Enterococcus spp. isolated from Hospital das Clínicas, FMUSP, São Paulo, Brazil

2003 ◽  
Vol 7 (3) ◽  
Author(s):  
H.H. Caiaffa Filho ◽  
G.D. Almeida ◽  
G.A. Oliveira ◽  
L. Sarahyba ◽  
E.M. Mamizuka ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Sao Paulo ◽  
São Paulo ◽  
Vancomycin Resistant Enterococcus ◽  
Enterococcus Spp ◽  
Vancomycin Resistant

scholarly journals Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil

2008 ◽  
Vol 50 (4) ◽  
pp. 195-198 ◽  
Author(s):  
Pedro Alves d'Azevedo ◽  
Guilherme H.C. Furtado ◽  
Eduardo A.S. Medeiros ◽  
Kelly Aline Santiago ◽  
Suzane Silbert ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Genetic Relatedness ◽  
Sao Paulo ◽  
São Paulo ◽  
Pfge Analysis ◽  
Predominant Species ◽  
Vana Gene ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

E. faecium was the first reported VRE species, carrying the vanA gene in Brazil. In spite of this, vancomycin-resistant E. faecalis has become the predominant species in Brazilian hospitals. The aim of this study was to evaluate the genetic relatedness of VREs isolated in a Brazilian teaching hospital eight years apart from its first isolation. We analyzed 38 VRE strains obtained from 81 surveillance cultures of patients admitted to the four largest intensive care units in Hospital São Paulo in February, 2006. Presence of the vanA gene was assayed by PCR and PFGE analysis was used for molecular characterization. All VRE strains carried the vanA gene. Two distinct clonal groups were observed among vancomycin-resistant E. faecalis. Vancomycin-resistant E. faecium belonged to five distinct clones were demonstrated by molecular typing. All of these clones were different from the first vancomycin-resistant enterococci clone isolated eight years ago in our hospital.

Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates from Hungary and Serbia

2008 ◽  
Vol 40 (10) ◽  
pp. 778-784 ◽  
Author(s):  
Balázs Libisch ◽  
Zorica Lepsanovic ◽  
Janetta Top ◽  
Mónika Muzslay ◽  
Marianne Konkoly-Thege ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Clinical Isolates ◽  
Vancomycin Resistant Enterococcus ◽  
Enterococcus Spp ◽  
Vancomycin Resistant

scholarly journals Identification and Molecular Characterization of Norovirus in São Paulo State, Brazil

2008 ◽  
Vol 39 (4) ◽  
pp. 619-622 ◽  
Author(s):  
Simone Guadagnucci Morillo ◽  
Cilli Audrey ◽  
Rita de Cássia Compagnoli Carmona ◽  
Maria do Carmo Sampaio Tavares Timenetsky
Keyword(s):  
Molecular Characterization ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State

scholarly journals Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Korea

2005 ◽  
Vol 43 (5) ◽  
pp. 2303-2306 ◽  
Author(s):  
K. S. Ko ◽  
J. Y. Baek ◽  
J.-Y. Lee ◽  
W. S. Oh ◽  
K. R. Peck ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Vancomycin Resistant Enterococcus ◽  
Vancomycin Resistant

scholarly journals Molecular Characterization of Mediterranean Spotted Fever Rickettsia Isolated From a European Traveler in the State of São Paulo, Brazil

2013 ◽  
Vol 20 (1) ◽  
pp. 54-56 ◽  
Author(s):  
Flávia S. Gehrke ◽  
Rodrigo N. Angerami ◽  
Mauro T. Marrelli ◽  
Eliana R. de Souza ◽  
Elvira M.M. do Nascimento ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Spotted Fever ◽  
Sao Paulo ◽  
The State ◽  
Mediterranean Spotted Fever ◽  
São Paulo ◽  
Spotted Fever Rickettsia

scholarly journals Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0171317 ◽  
Author(s):  
Patrick Eberechi Akpaka ◽  
Shivnarine Kissoon ◽  
Clyde Wilson ◽  
Padman Jayaratne ◽  
Ashley Smith ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Vancomycin Resistant Enterococcus ◽  
Vancomycin Resistant

scholarly journals Molecular characterization of two rocio flavivirus strains isolated during the encephalitis epidemic in são paulo state, brazil and the development of a one-step rt-pcr assay for diagnosis

2008 ◽  
Vol 50 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Terezinha Lisieux Moraes Coimbra ◽  
Raimundo N. Santos ◽  
Selma Petrella ◽  
Teresa Keico Nagasse-Sugahara ◽  
Silvana Beres Castrignano ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Clinical Manifestations ◽  
Cross Reactivity ◽  
Sao Paulo ◽  
São Paulo ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Paulo State ◽  
São Paulo State

Rocio virus (ROCV) was responsible for an explosive encephalitis epidemic in the 1970s affecting about 1,000 residents of 20 coastland counties in São Paulo State, Brazil. ROCV was first isolated in 1975 from the cerebellum of a fatal human case of encephalitis. Clinical manifestations of the illness are similar to those described for St. Louis encephalitis. ROCV shows intense antigenic cross-reactivity with Japanese encephalitis complex (JEC) viruses, particularly with Ilheus (ILHV), St. Louis encephalitis, Murray Valley and West Nile viruses. In this study, we report a specific RT-PCR assay for ROCV diagnosis and the molecular characterization of the SPAn37630 and SPH37623 strains. Partial nucleotide sequences of NS5 and E genes determined from both strains were used in phylogenetic analysis. The results indicated that these strains are closely related to JEC viruses, but forming a distinct subclade together with ILHV, in accordance with results recently reported by Medeiros et al. (2007).

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