scholarly journals Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis

2002 ◽  
Vol 25 (2) ◽  
pp. 243-250 ◽  
Author(s):  
Ana Maria Pujol Vieira dos Santos ◽  
Aida T. Santos Matsumura ◽  
Sueli Teresinha Van Der Sand
Keyword(s):  
Genetic Diversity ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Intraspecific Genetic Diversity

scholarly journals Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

2013 ◽  
Vol 13 (2) ◽  
pp. 73-78
Author(s):  
Jarina Joshsi ◽  
Lumanti Manandhar ◽  
Patima Shrestha ◽  
Rani Gupta ◽  
Rojlina Manadhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Neighbor Joining ◽  
German Shepherd ◽  
Polymorphic Loci ◽  
Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

Genetic diversity of potato determined by random amplified polymorphic DNA analysis

1996 ◽  
Vol 15 (9) ◽  
pp. 662-667 ◽  
Author(s):  
L.M. Kawchuk ◽  
G.C. Kozub ◽  
J.D. Armstrong ◽  
D. R. Lynch ◽  
T. Demeke
Keyword(s):  
Genetic Diversity ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna

Genetic diversity in sunflower (Helianthus annuus L.) as revealed by random amplified polymorphic DNA analysis

1994 ◽  
Vol 45 (7) ◽  
pp. 1319 ◽  
Author(s):  
WR Lawson ◽  
RJ Henry ◽  
JK Kochman ◽  
GA Kong
Keyword(s):  
Genetic Diversity ◽  
Ribosomal Rna ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Efficient Tool ◽  
Breeding Programs ◽  
Breeding Lines ◽  
Sunflower Genome ◽  
Pair Wise Comparison ◽  
Selection Of

A cross-section of sunflower genotypes grown in Australia including commercial cultivars (Suncross 40R, Hysun 33, Hysun 45CQ, Advance, DK3873), breeding lines (Sunfola, S37- 388, PhRR3, HA-R2, MC29, MC69, S37-388RR), wild sunflower varieties (H. annuus, H. argophyllus), a distantly related species (Tithonia diversifolia), and a hexaploid/diploid cross (H. tuberosus L.x H. annuus L.) were assessed for genetic diversity using RAPD (Random Amplified Polymorphic DNA) analysis. A considerable amount of polymorphism was revealed. Of the total of 158 markers amplified, 133 were polymorphic for at least one pair-wise comparison within the 16 genotypes. Overall, 33% dissimilarity was detected, with an average of 27% dissimilarity revealed among the hybrids and breeding lines, which exhibited 38% dissimilarity to the wild varieties H. annuus and H. tuberosus, and 51% dissimilarity to Tithonia and H. tuberosus x H, annuus. PCR of the 5S ribosomal RNA gene spacer region did not reveal any polymorphisms among the cultivated and breeding lines, but did distinguish between H. tuberosus and the other wild species. This survey of a selection of sunflower genotypes indicates that the genetic base of domesticated sunflower may be quite wide. These results suggest that RAPD methodology will provide an efficient tool for the analysis of the sunflower genome, in particular in breeding programs.

Genetic diversity in cultivated Osteospermum as revealed by random amplified polymorphic DNA analysis

2000 ◽  
Vol 119 (4) ◽  
pp. 351-355 ◽  
Author(s):  
P. Faccioli ◽  
V. Terzi ◽  
N. Pecchioni ◽  
T. Berio ◽  
A. Giovannini ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna

scholarly journals Genetic Diversity of Two Tilapia Species (Oreochromis Niloticus and Sarthrodon Galilaeus) Using Random Amplified Polymorphic DNA

2020 ◽  
Vol 8 (1) ◽  
pp. 22-28
Author(s):  
Omeima M. Omer ◽  
Abd El Wahab H. Abdalla ◽  
Zuheir N. Mahmoud
Keyword(s):  
Genetic Diversity ◽  
Oreochromis Niloticus ◽  
Dna Analysis ◽  
Nile Blue ◽  
Random Amplified Polymorphic Dna ◽  
Tissue Samples ◽  
Rapd Pcr ◽  
White Nile ◽  
Sarotherodon Galilaeus ◽  
High Level

Introduction: In this study, the genetic variability in eight populations of Oreochromis niloticus and seven populations of Sarotherodon galilaeus was estimated using molecular markers. Materials and Methods: Fish specimens were collected from eight sites representing the White Nile, Blue Nile and the River Nile. Tissue samples from gills and dorsal fin were removed from individual specimens and preserved separately in absolute ethanol prior to molecular analysis by RAPD-PCR using eight primers. DNA analysis using OPA-04, OPA-13, OPA-03, OPA-06, OPA-07, OPA-09, OPA-10 and RAPD-8 produced different bands for each. Results and Discussion: The total bands generated by the primers were: 17, 16, 18, 12, 12, 14, 14, and 17. They were in the range of 100 to 1020 bp. Levels of variability were estimated by the proportion of polymorphic bands obtained by each primer within a population. The range of variability was wider in O. niloticus (46.0 to 91.7) compared to S. galilaeus (56.2 to 83.3). The dendrogram obtained differentiated the populations into 22 sub-clusters. Oreochromis niloticus from Al Kalakla exhibited a high level of genetic diversity. This diversity is evident among and within the studied populations, as estimated by RAPD-PCR techniques. Conclusion: To promote tilapia production, the study recommended increasing genetic variation within broodstocks by crossing high similarity breeds with low similarity ones.

scholarly journals Environmental DNA analysis shows high potential as a tool for estimating intraspecific genetic diversity in a wild fish population

2019 ◽  
Author(s):  
Satsuki Tsuji ◽  
Atsushi Maruyama ◽  
Masaki Miya ◽  
Masayuki Ushio ◽  
Hirotoshi Sato ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Water Sample ◽  
Sanger Sequencing ◽  
Large Scale ◽  
High Throughput Sequencing ◽  
Dna Analysis ◽  
Environmental Dna ◽  
Intraspecific Diversity ◽  
Survey Method ◽  
Intraspecific Genetic Diversity

AbstractEnvironmental DNA (eDNA) analysis has recently been used as a new tool for estimating intraspecific diversity. However, whether known haplotypes contained in a sample can be detected correctly using eDNA-based methods has been examined only by an aquarium experiment. Here, we tested whether the haplotypes of Ayu fish (Plecoglossus altivelis altivelis) detected in a capture survey could also be detected from an eDNA sample derived from the field that contained various haplotypes with low concentrations and foreign substances. A water sample and Ayu specimens collected from a river on the same day were analysed by eDNA analysis and Sanger sequencing, respectively. The 10 L water sample was divided into 20 filters for each of which 15 PCR replications were performed. After high-throughput sequencing, denoising was performed using two of the most widely used denoising packages, UNOISE3 and DADA2. Of the 42 haplotypes obtained from the Sanger sequencing of 96 specimens, 38 (UNOISE3) and 41 (DADA2) haplotypes were detected by eDNA analysis. When DADA2 was used, except for one haplotype, haplotypes owned by at least two specimens were detected from all the filter replications. This study showed that the eDNA analysis for evaluating intraspecific genetic diversity provides comparable results for large-scale capture-based conventional methods, suggesting that it could become a more efficient survey method for investigating intraspecific genetic diversity in the field.

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