scholarly journals An AFLP marker linked to the Pm-1 gene that confers resistance to Podosphaera xanthii race 1 in Cucumis melo

2008 ◽  
Vol 31 (2) ◽  
pp. 547-550 ◽  
Author(s):  
Ana Paula Matoso Teixeira ◽  
Fatima Aparecida da Silva Barreto ◽  
Luis Eduardo Aranha Camargo
Keyword(s):  
Cucumis Melo ◽  
Aflp Marker ◽  
Podosphaera Xanthii ◽  
Race 1

scholarly journals Occurrence of Podosphaera xanthii Race 2 on Cucumis melo in Brazil

Plant Disease ◽  
2004 ◽  
Vol 88 (10) ◽  
pp. 1161-1161 ◽  
Author(s):  
R. F. Kobori ◽  
O. Suzuki ◽  
R. Wierzbicki ◽  
P. T. Della Vecchia ◽  
L. E. A. Camargo
Keyword(s):  
Cucumis Melo ◽  
Fungal Growth ◽  
Sao Paulo ◽  
Podosphaera Xanthii ◽  
São Paulo ◽  
Erysiphe Cichoracearum ◽  
Summer Squash ◽  
Golovinomyces Cichoracearum ◽  
Race 1 ◽  
Race 2

Powdery mildew is an important disease of melons (Cucumis melo L.) cultivated in greenhouses in Brazil. Currently, there are 5 races of Podosphaera xanthii (formerly known as Sphaerotheca fuliginea) and 2 races of Golovinomyces cichoracearum (formerly known as Erysiphe cichoracearum) described on melons worldwide, but only race 1 of P. xanthii has been reported in Brazil (1). However, typical whitish powdery fungal growth was observed on an experimental hybrid yellow melon resistant to race 1 of P. xanthii during the summer of 2000 in a greenhouse in Bragança Paulista, State of São Paulo. Conidia collected from diseased leaves were spread onto 0.5% water agar medium and maintained at 22°C for 24 h with 12 h of light and 12 h of darkness. Most of the germinated conidia displayed fibrosin inclusion bodies when observed in a solution of 3% potassium hydroxide (KOH), and approximately 1 of 50 also displayed forked germ tubes. These features allowed us to identify P. xanthii as the causal agent. Conidia raised on the susceptible yellow melon ‘Amarelo CAC’ were used to inoculate cotyledons of the differential melon lines (2) ‘Hale's Best Jumbo’ (susceptible to races 1, 2, and 3 of P. xanthii), ‘PMR-45’ (resistant to race 1 and susceptible to races 2 and 3), and ‘PMR-6’ (resistant to races 1 and 2 and susceptible to race 3). Inoculations were performed on 10 plants of each differential line and replicated four times. The presence or absence of symptoms was evaluated 18 days after inoculation. ‘Hale's Best Jumbo’ and ‘PMR-45’ were rated as susceptible while ‘PMR-6’ was rated as resistant, thus indicating the presence of race 2 of P. xanthii in Brazil. During field surveys from 2001 to 2003, this race was found on squash (Cucurbita moschata), summer squash (C. pepo), and melons in São Paulo. References: (1) F. J. B. Reifschneider et al. Plant Dis. 69:1069, 1985. (2) C. E. Thomas et al. Cucurbit Genet. Coop. 7:126, 1984.

scholarly journals Melon-Powdery Mildew Interactions Reveal Variation in Melon Cultigens and Podosphaera xanthii Races 1 and 2

2006 ◽  
Vol 131 (1) ◽  
pp. 59-65 ◽  
Author(s):  
James D. McCreight
Keyword(s):  
Powdery Mildew ◽  
Virulence Factors ◽  
Cucumis Melo ◽  
Podosphaera Xanthii ◽  
Sphaerotheca Fuliginea ◽  
Race 1 ◽  
New Races ◽  
Race 2 ◽  
Serious Disease ◽  
Greenhouse Tests

Powdery mildew is a serious disease of melon (Cucumis melo L.) worldwide. Twenty-two melon cultigens have been used to define 22 reported races of the pathogen Podosphaera xanthii (sect. Sphaerotheca) xanthii (Castag.) U. Braun & N. Shish. Comb. nov. [syn. Sphaerotheca fuliginea (Schlecht. ex Fr.) Poll.]. Discrepancies in the reactions of eight cultigens to populations of P. xanthii races 1 and 2 in California, Japan, and Spain revealed genetic differences among them that can be used to differentiate P. xanthii race 1 and 2 populations in these countries. Implicit in these results is the existence of previously unknown virulence factors in these populations of P. xanthii races 1 and 2 that permit designation of new races of P. xanthii on melon. Synthesis of these results with previous reports resulted in the identification of 28 putative races of P. xanthii on melon that include eight variants of race 1 and six variants of race 2. Six of the cultigens exhibited resistant blisters in response to heavy infection by P. xanthii in field and greenhouse tests.

Histological aspects of Cucumis melo PI 313970 resistance to Podosphaera xanthii and Golovinomyces cichoracearum

2009 ◽  
Vol 116 (4) ◽  
pp. 169-176 ◽  
Author(s):  
M. Sedlá ◽  
A. Lebeda ◽  
P. Mikšíková ◽  
M. Duchoslav ◽  
B. Sedláková ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
Podosphaera Xanthii ◽  
Golovinomyces Cichoracearum

scholarly journals FIELD EVIDENCE FOR A NEW RACE OF POWDERY MILDEW ON MELON

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 888a-888 ◽  
Author(s):  
James D. McCreight ◽  
Michael D. Coffey ◽  
Thomas A. Turini ◽  
Michael E. Matheron
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Genetic Resistance ◽  
Podosphaera Xanthii ◽  
Sphaerotheca Fuliginea ◽  
Imperial Valley ◽  
Field Evidence ◽  
Race 1 ◽  
New Race ◽  
The U.S

Races 1 and 2 of Podosphaera xanthii (syn. Sphaerotheca fuliginea) were defined in Imperial Valley, Calif. 1938 when P. xanthii overcame genetic resistance in `PMR 45'. Race 3 was first observed in the U.S. in 1976 in Texas; 15 additional races of P. xanthii have been reported in the literature since 1996. Races 1 and 2 have been common in Arizona and California based upon the effectiveness of the powdery mildew resistance genes in commercially available melon cultivars grown in these states. Field data from 11 commonly used melon P. xanthii race differentials in 2001 and 2002 indicated the presence of race 1 in the Imperial Valley and San Joaquin Valley of California, and Yuma, Arizona. In spring 2003, the powdery mildew race situation changed. The first evidence was the occurrence of a severe and widespread infection of powdery mildew in a commercial cantaloupe field. The 11 powdery mildew race differentials were susceptible to powdery mildew in a nearby replicated field test. PI 313970, a melon from India, was resistant to this apparent new race of powdery mildew.

scholarly journals Development of Sequence-specific Primers that Amplify a 1.5-kb DNA Marker for Race 1 Fusarium Wilt Resistance in Cucumis melo L.

HortScience ◽  
1998 ◽  
Vol 33 (2) ◽  
pp. 291-292 ◽  
Author(s):  
W. Patrick Wechter ◽  
Ralph A. Dean ◽  
Claude E. Thomas
Keyword(s):  
Fusarium Wilt ◽  
Cucumis Melo ◽  
Specific Primers ◽  
Breeding Programs ◽  
Breeding Lines ◽  
Race 1 ◽  
Dna Fragment ◽  
Polymerase Chain ◽  
Cucumis Melo L

Two 24-mer primers, MUSKFOM I and MUSKFOM II, were developed that amplify a 1.5-kb DNA fragment in race 1 Fusarium wilt resistant muskmelon (Cucumis melo L.), but not in race 1 susceptible germplasm tested. Three race 1 resistant cultivars and two race 1 resistant breeding lines as well as eight race 1 susceptible lines were analyzed using the two sequence-specific primers in the polymerase chain reaction. These primers should prove valuable for nondestructive determination of Fom 2 gene introgression in breeding programs.

scholarly journals Genes for Resistance to Powdery Mildew Races 1 and 2U.S. in Melon PI 313970

HortScience ◽  
2003 ◽  
Vol 38 (4) ◽  
pp. 591-594 ◽  
Author(s):  
James D. McCreight
Keyword(s):  
Powdery Mildew ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Sphaerotheca Fuliginea ◽  
Limited Data ◽  
Race 1 ◽  
Resistance Reaction ◽  
Greenhouse Tests

Powdery mildew is a major problem in melon (Cucumis melo L.) production worldwide. Three genes for resistance to Sphaerotheca fuliginea (Schlecht. ex Fr.) Poll. race 1 and race 2U.S. were identified in growth chamber and greenhouse tests in the cross of PI 313970 × `Top Mark'. A recessive gene conditioned resistance of true leaves to race 1. A recessive gene appeared to condition resistance of cotyledons to race 2U.S., although a second recessive gene may be involved. A semi-dominant gene conditioned resistance of true leaves to race 2U.S. Limited data suggested linkage of the recessive gene for resistance to race 1 and the semi-dominant gene for resistance to race 2U.S. The resistance reaction of PI 313970 to infection of true leaves by race 2U.S. included water-soaked spots and resistant blisters, but segregation data for the resistant blister reaction were inconclusive. Allelic relationships of these genes with previously reported melon powdery mildew resistance genes remain to be determined.

scholarly journals First report of powdery mildew caused by Podosphaera xanthii race 1 on watermelon in China

2021 ◽  
Author(s):  
Haonan Cui ◽  
Zicheng Zhu ◽  
Zhuo Ding ◽  
Yuanzuo Lv ◽  
Lei Sun ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Podosphaera Xanthii ◽  
First Report ◽  
Race 1

scholarly journals Estudo da diversidade genética de Podosphaera xanthii através de marcadores AFLP e seqüências ITS

2011 ◽  
Vol 37 (2) ◽  
pp. 94-100 ◽  
Author(s):  
Erika Sayuri Naruzawa ◽  
Raphaelle Komatsu Dalla Vale ◽  
Cynthia Maria Silva ◽  
Luis Eduardo Aranha Camargo
Keyword(s):  
Cucumis Melo ◽  
Podosphaera Xanthii ◽  
Para Determinar ◽  
Cucumis Melo L

O meloeiro (Cucumis melo L.) é uma frutífera largamente cultivada no Brasil, principalmente no nordeste brasileiro, onde é produzida principalmente para a exportação. Plantas da família do meloeiro, como pepino e abóbora, podem ser severamente afetadas pelo oídio, causado por Podosphaera xanthii.. Este fungo apresenta diversas raças fisiológicas cuja correta identificação é importante para o manejo da doença, já que o uso de variedades resistentes é o método mais eficaz de seu controle. No entanto, a identificação destas raças por meio da prática tradicional de inoculações em uma série diferenciadora de variedades de meloeiro é laboriosa e passível de erros. Devido a isso, um método alternativo seria o uso de marcadores moleculares para determinar de forma rápida a identidade das raças. O objetivo deste estudo foi o de analisar a variabilidade entre isolados de P. xanthii previamente classificados em raças através da técnica de AFLP e do seqüenciamento da região ITS 5.8S do rDNA. A partir dos marcadores AFLP obteve-se um dendrograma no qual não houve separação dos isolados quanto às suas raças, origem geográfica ou hospedeiro de origem. Com esta técnica verificou-se alta variabilidade entre isolados, com similaridade genética máxima de 69% e similaridade mínima de 23%. Ao contrário da informação gerada por AFLP, não foi observada variação na sequência da região ITS 5.8S entre isolados. Desta forma, a análise por AFLP indicou que os isolados tem composição genética heterogênea muito embora este fato não tenha sido evidenciado pelo sequenciamento da região ITS.

scholarly journals CmPMRl and CmPMrs Were Responsible for Resistance to Powdery Mildew Caused by Podosphaera Xanthii Race 1 in Melon

2021 ◽  
Author(s):  
Haonan Cui ◽  
Chao Fan ◽  
Zhuo Ding ◽  
Xuezheng Wang ◽  
Lili Tang ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Candidate Gene ◽  
Cucumis Melo ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Cellular Membrane ◽  
Epistatic Effect ◽  
Marker Assisted Breeding ◽  
Stem Resistance ◽  
Race 1

Abstract Cucumis melo L. is an economically important crop, the production of which is threatened by the prevalence of melon powdery mildew (PM) infections. We herein utilized the MR-1 (P1; resistant to PM) and M4-7 (P2; susceptible to PM) accessions to assess the heritability of PM (race 1) resistance in these melon plants. PM resistance in MR-1 leaves was linked to a dominant gene (CmPMRl), whereas stem resistance was under the control of a recessive gene (CmPMrs), with the dominant gene having an epistatic effect on the recessive gene. The CmPMRl gene was mapped to a 50 Kb interval on chromosome 12, while CmPMrs was mapped to an 89 Kb interval on chromosome 10. The CmPMRl candidate gene MELO3C002441 and the CmPMrs candidate gene MELO3C012438 were identified through sequence alignment, functional annotation, and expression pattern analyses of all genes within these respective intervals. MELO3C002441 and MELO3C012438 were both localized to the cellular membrane and were contained conserved NPR gene-like and MLO domains, respectively, which were linked to PM resistance. In summary, we identified patterns of PM resistance in the disease-resistant MR-1 melon cultivar, and conducted finally-mapping to identify two putative genes linked to resistance. Our results offer new genetic resources and markers guide the future molecular marker-assisted breeding of PM-resistant melon.

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