scholarly journals Production and properties of Xylanase from thermophilic Bacillus sp.

2002 ◽  
Vol 45 (4) ◽  
pp. 413-418 ◽  
Author(s):  
Carlos Alberto Martins Cordeiro ◽  
Meire Lelis Leal Martins ◽  
Angélica Bárbara Luciano ◽  
Roberta Freitas da Silva
Keyword(s):  
Bacillus Sp ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Original Activity ◽  
Sequence Comparisons ◽  
Liquid Cultures ◽  
Local Soil ◽  
Beechwood Xylan ◽  
Optimum Ph ◽  
Crude Enzyme Solution

An aerobic, thermophilic, xylanolytic bacterium was isolated from local soil. The results of 16S rRNA sequence comparisons indicated that the isolate was closely related to Bacillus caldoxylolyticus and Bacillus sp strain AK1. These organisms exhibited 94% levels of ribossomal DNA sequence homology. Studies on the xylanase characterisation from liquid cultures grown on beechwood xylan revealed that the enzyme retained 100% of activity for 2 hours at temperatures ranging from 30 to 50º C, while at 60, 70 and 100º C, 10%, 11% and 29% of the original activities were lost, respectively. The optimum pH of the enzyme was found to be between 6.5 and 7.0. After incubation of crude enzyme solution for 24 hours at 25º C and at pH 5.5 to 8.0, a decrease of about 12% of its original activity was observed.

The use of functional analysis of the ribosome as a tool to determine archaebacterial phylogeny

1989 ◽  
Vol 35 (1) ◽  
pp. 141-147 ◽  
Author(s):  
R. Amils ◽  
L. Ramírez ◽  
J. L. Sanz ◽  
I. Marín ◽  
A. G. Pisabarro ◽  
...  
Keyword(s):  
Functional Analysis ◽  
16S Rrna ◽  
Phylogenetic Tree ◽  
Functional Information ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Sequence Comparisons ◽  
Free System ◽  
Translation Apparatus ◽  
Evolutionary Clock

Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were used to cluster the different archaebacteria analyzed. This cluster resembles the phylogenetic tree generated by 16S rRNA sequence comparisons. These results strongly suggest that functional analysis of an appropriate evolutionary clock, such as the ribosome, is of intrinsic phylogenetic value. More importantly, they indicate that the study of the nexus between genotypic and phenotypic (functional) information may shed considerable light on the evolution of the protein synthetic machinery.Key words: antibiotics, ribosomes, archaebacteria, phylogeny, functional analysis.

scholarly journals OPTIMIZATION OF CULTURE CONDITION FOR IAA RODUCTION BY BACILLUS SP. ISOLATED FROM CASSAVA FIELD OF VIETNAM

2021 ◽  
Vol 59 (3) ◽  
pp. 312
Author(s):  
Pham Viet Cuong ◽  
Nguyen Phuong Hoa
Keyword(s):  
16S Rrna ◽  
Yeast Extract ◽  
Culture Condition ◽  
Culture Conditions ◽  
Maximum Amount ◽  
Bacillus Sp ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Iaa Production ◽  
Optimization Of Culture Conditions

Abstract. The bacteria capable producing IAA were isolated from roots of cassava growing in Tay Ninh region (Southern of Vietnam). The isolate producing highest amount of IAA was chosen for identification and optimization of culture conditions for IAA production. By analyzing 16S rRNA sequence, the selected isolate was identified as Bacillus sp. DTAN-M5. The influence of different parameters on IAA production of this strains was evaluated by “one factor at a time” method and the obtained results showed that the best conditions for IAA production of DTAN-M5 were medium with following components: 1% peptone; 0.5% sucrose , 0.6% yeast extract, 0.5% NaCl, pH 7.1, supplemented with 2mg/L L-tryptophan, incubation in a shaker 150 rpm at 30o C for 84 hours. The maximum amount of IAA was 84,13 µg/mL

scholarly journals A novel filamentous Bacillus sp., strain NAF001, forming endospores and budding cells The GenBank/EMBL/DDBJ accession number for the 16S rRNA sequence reported in this paper is AB049195.

Microbiology ◽  
2001 ◽  
Vol 147 (6) ◽  
pp. 1415-1423 ◽  
Author(s):  
Vasudevan P Ajithkumar ◽  
Bindu Ajithkumar ◽  
Koji Mori ◽  
Kazuhiro Takamizawa ◽  
Ryozo Iriye ◽  
...  
Keyword(s):  
16S Rrna ◽  
Bacillus Sp ◽  
Accession Number ◽  
Rrna Sequence ◽  
16S Rrna Sequence

scholarly journals Isolation, purification and characterization of mannan degrading enzyme from a new isolate Acinetobacter baummanni

2021 ◽  
Vol 924 (1) ◽  
pp. 012078
Author(s):  
D Muzaki ◽  
E Zubaidah ◽  
S Santoso ◽  
A Sutrisno
Keyword(s):  
Enzyme Activity ◽  
Guar Gum ◽  
Relative Activity ◽  
Purification And Characterization ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Ammonium Sulphate Precipitation ◽  
Optimum Ph ◽  
16S Rrna Sequence Analysis

Abstract A mannan-degrading microbe was isolated from rotting porang tubers (Amorphophallus muelleri Blume). Molecular identification using 16S-rRNA sequence analysis revealed that the isolate showed 99.67% similarity with Acinetobacter baummanni. A crude enzyme from ammonium sulphate precipitation was used for preliminary characterization. The characterization results showed that the enzyme activity is optimum at 45 °C, and stable at 35-50 °C, while the optimum pH is 7, and stable at pH 5-7. The substrate with the highest relative activity was found in guar gum which was 137.512%. The enzyme activity was inhibited by Ca, Na, K ions, and increased by Mn2+ ions.

scholarly journals Differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis analysis and 16S rRNA sequence comparisons

1996 ◽  
Vol 136 (2) ◽  
pp. 181-186 ◽  
Author(s):  
Thad B. Stanton ◽  
Darren J. Trott ◽  
Jae I. Lee ◽  
Andrew J. McLaren ◽  
David J. Hampson ◽  
...  
Keyword(s):  
16S Rrna ◽  
Enzyme Electrophoresis ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Sequence Comparisons ◽  
Multilocus Enzyme Electrophoresis

scholarly journals Studying on the antifungal activityof the actinomycete XK1 isolatedfrom growing orange’s soil against pathogenic fungi causing disease in citrus

2021 ◽  
Vol 63 (5) ◽  
pp. 41-45
Author(s):  
Bao Tram Tran ◽  
◽  
Thi Hien Nguyen ◽  
Binh Minh Tran ◽  
Thi Thuy Linh Nguyen ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Morphological Characteristics ◽  
Pathogenic Fungi ◽  
Fungal Species ◽  
Plant Diseases ◽  
Fruit Rot ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Liquid Cultures ◽  
Actinomycete Strain

Streptomyces are considered to be one of the actinomycetes genera having potential in the production of agricultural probiotics because they are safe and have strong antagonism to many bacterial and fungal species that cause plant diseases. In this study, 18 strains of actinomycetes were isolated from growing oranges’ soil in Ha Giang province, of which the actinomycete strain XK1 was considered to be strongly resistant to the fungi Penicillium digitatum and Colletotrichum gloeosporioides causing fruit rot and shedding in citrus. Based on morphological characteristics and 16S rRNA sequence, the strain XK1 was determined to belong to the Streptomyces albulus. On MT2 medium with pH=6, after five days of shaking cultivation at 30°C, the liquid cultures of S. albulus XK1 showed the strongest resisting activity to P. digitatum and C. gloeosporioides. At the same time, the broth cultures of S. albulus XK1 showed inhibitory activity against P. digitatum in oranges. The actinomycete strain S. albulus XK1 was selected base on strong antifungal ability against P. digitatum and C. gloeosporioides and had a potential for application in the production of probiotics used to control fruit rot and shedding in citrus.

scholarly journals Pseudomonas eucalypticola sp. nov., a producer of antifungal agents isolated from Eucalyptus dunnii leaves

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yujing Liu ◽  
Zhang Song ◽  
Hualong Zeng ◽  
Meng Lu ◽  
Weiyao Zhu ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Antifungal Agents ◽  
Novel Species ◽  
Phytopathogenic Fungi ◽  
Strictly Aerobic ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Eucalyptus Dunnii ◽  
16S Rrna Sequence Analysis

AbstractPseudomonas are ubiquitously occurring microorganisms and are known for their ability to produce antimicrobials. An endophytic bacterial strain NP-1 T, isolated from Eucalyptus dunnii leaves, exhibits antifungal properties against five tested phytopathogenic fungi. The strain is a Gram-negative rod-shaped bacterium containing a single polar flagellum. It is strictly aerobic, grows at 4–37 °C, 2–5% NaCl, and pH 3–7. The 16S rRNA sequence analysis showed that NP-1 T belongs to the Pseudomonas genus. Phylogenetic analysis based on four concatenated partial genes (16S rDNA, gyrB, rpoB and rpoD) and the phylogenomic tree indicated that NP-1 T belongs to Pseudomonas fluorescens lineage but is distinct from any known Pseudomonas species. The G + C mol % of NP-1 T genome is 63.96, and the differences between NP-1 T and related species are larger than 1. The digital DNA-DNA hybridization and tetranucleotide signatures are 23.8 and 0.97, which clearly separates strain NP-1 T from its closest neighbours, Pseudomonas coleopterorum and Pseudomonas rhizosphaerae. Its phenotypic and chemotaxonomic features confirmed its differentiation from related taxa. The results from this polyphasic approach support the classification of NP-1 T as a novel species of Pseudomonas, and the name of Pseudomonas eucalypticola is thus proposed for this strain, whose type is NP-1 T (= CCTCC M2018494T = JCM 33572 T).

scholarly journals 16S rRNA Sequence-based Rapid and Sensitive Detection of Aquatic Bacteria by On-chip Hybridization Following Multiplex PCR

2008 ◽  
Vol 54 (1) ◽  
pp. 123-128 ◽  
Author(s):  
Tomoaki Ichijo ◽  
Nobuyasu Yamaguchi ◽  
Katsuji Tani ◽  
Masao Nasu
Keyword(s):  
16S Rrna ◽  
Multiplex Pcr ◽  
Sensitive Detection ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Aquatic Bacteria ◽  
On Chip
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