scholarly journals Genetic Diversity of Plant Growth Promoting Rhizobacteria of Bacillus sp. Based on 16S rRNA Sequence and Amplified rDNA Restriction Analysis

2009 ◽  
Vol 3 (1) ◽  
pp. 12-16 ◽  
Author(s):  
SYAMSUL BAHRI SYAMSUL BAHRI ◽  
ARIS TRI WAHYUDI ◽  
NISA RACHMANIA MUBARIK
Keyword(s):  
Genetic Diversity ◽  
16S Rrna ◽  
Restriction Analysis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Sp ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Amplified Rdna Restriction Analysis

scholarly journals Molecular Characterization of Rhizobacteria (Isolate EO-4) as Potential Solvent of Phosphate in-vitro from the Mangrove Ecosystem of Teluk Awur, Jepara-Indonesia

2020 ◽  
Vol 5 (01) ◽  
pp. 22-33
Author(s):  
Eka Oktaviani ◽  
Rejeki Siti Ferniah ◽  
Arina Tri Lunggani
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
Molecular Characterization ◽  
Plant Growth Promoting Rhizobacteria ◽  
Mangrove Ecosystem ◽  
16S Rrna Sequence ◽  
Plant Growth Promoting ◽  
Phosphate Solubilizing

Rejuvenation and conservation of mangrove ecosystems, especially the Teluk Awur mangrove ecosystem, Jepara Regency, can be done by using bacteria that are capable of supporting plant growth or called Plant Growth Promoting Rhizobacteria (PGPR). One of the mechanisms that support plant growth by the PGPR group is phosphate dissolving activity, because the phosphate in the soil is in the form of a compound that is difficult for plants to absorb. This study aims to determine the character of Rhizobacter isolates as a superior phosphate solvent in dissolving phosphates in-vitro from the Teluk Awur mangrove ecosystem, namely isolates coded EO-4. These isolates have similar microbiological and biochemical characters to the genus Enterobacter. Molecular characterization of isolates was carried out using the Polymerase Chain Reaction (PCR) method - 16S rRNA sequence analysis (comparing with 16S rRNA sequences in gene banks). The results showed that the phosphate-solubilizing rhizobacteria that were isolated had the same base pair percentage of 48% with Enterobacter pyrinus (access number NR_028875).

scholarly journals OPTIMIZATION OF CULTURE CONDITION FOR IAA RODUCTION BY BACILLUS SP. ISOLATED FROM CASSAVA FIELD OF VIETNAM

2021 ◽  
Vol 59 (3) ◽  
pp. 312
Author(s):  
Pham Viet Cuong ◽  
Nguyen Phuong Hoa
Keyword(s):  
16S Rrna ◽  
Yeast Extract ◽  
Culture Condition ◽  
Culture Conditions ◽  
Maximum Amount ◽  
Bacillus Sp ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Iaa Production ◽  
Optimization Of Culture Conditions

Abstract. The bacteria capable producing IAA were isolated from roots of cassava growing in Tay Ninh region (Southern of Vietnam). The isolate producing highest amount of IAA was chosen for identification and optimization of culture conditions for IAA production. By analyzing 16S rRNA sequence, the selected isolate was identified as Bacillus sp. DTAN-M5. The influence of different parameters on IAA production of this strains was evaluated by “one factor at a time” method and the obtained results showed that the best conditions for IAA production of DTAN-M5 were medium with following components: 1% peptone; 0.5% sucrose , 0.6% yeast extract, 0.5% NaCl, pH 7.1, supplemented with 2mg/L L-tryptophan, incubation in a shaker 150 rpm at 30o C for 84 hours. The maximum amount of IAA was 84,13 µg/mL

scholarly journals Characterization of potential plant growth-promoting rhizobacteria isolated from sunflower (Helianthus annuus L.) in Iran

2019 ◽  
Vol 70 (4) ◽  
pp. 268-277
Author(s):  
Nabi Khezrinejad ◽  
Gholam Khodakaramian ◽  
Fatemeh Shahryari
Keyword(s):  
Genetic Diversity ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pgp Traits ◽  
North West ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Identification And Characterization

Purpose This study aims to characterize plant growth-promoting rhizobacteria (PGPR) in sunflowers growing in different locations at North West of Iran. Materials and methods Sunflower plants were collected from different regions of West Azarbaijan, and rhizospheric bacterial strains were isolated and screened for PGP traits. Identification and characterization of the PGPR were conducted based on 16s rDNA sequences and phenotypic analysis, the strains clustered for genetic diversity by rep-PCR method. Results Among the 80 bacterial isolates, 20 showed PGP traits and were selected for other potentials. All the selected isolates produced indole-3-acetic acid at the rate of 9.2–33.7 mg/ml. In addition, 13, 15, 12, and 16 were positive for phosphate solubilization, siderephore, hydrogen cyanide, and ammonia production, respectively. The results from a subsequent pot experiment indicated that PGPRs distinctly increased sun flower shoot and root length, shoot and root fresh weight, as well as shoot and root dry weight. Based on 16S rDNA sequences and biochemical and physiological characteristics, 20 PGPRs were identified as Pseudomonas fluorescens (five isolates), Pseudomonas aeruginosa (four isolates), Pseudomonas geniculata (one isolate), Bacillus subtilis (four isolates), Bacillus pumilus (two isolates), Stenotrophomonas maltophilia (two isolates), and Brevibacterium frigoritolerans (two isolates). In rep-PCR, PGPR isolates were differentiated into seven clusters (A–G) at 65% similarity level. These results demonstrated the existence of a considerable species richness and genetic diversity among PGPRs isolated from different regions of North West of Iran. Conclusions To the best of our knowledge, this is first report for the identification and characterization of B. frigoritolerans as PGPR in sunflower plants.

scholarly journals Effect of plant growth promoting rhizobacteria (PGPR) and IBA treatments on rooting in cuttings of apple (Malus × domestica Borkh.) clonal rootstock Merton 793

2017 ◽  
Vol 9 (2) ◽  
pp. 1135-1138
Author(s):  
Pramod Verma ◽  
P. S. Chauhan ◽  
J. S. Chandel
Keyword(s):  
Plant Growth ◽  
Bacillus Licheniformis ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Sp ◽  
Main Shoot ◽  
Primary Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Different Strains

The preliminary studies on the effect of different strains of plant growth promoting rhizobacteria (PGPR) alone and in combination with IBA at 1000 ppm on rooting in cuttings of apple clonal rootstock Merton 793 were carried out during 2012-13. The PGPR strains (RG (1)3 – Bacillus sp.), B6 – Bacillus licheniformis and R3 (3) – Sirretia sp. alone failed to induce rooting response in cuttings of apple clonal rootstock Merton 793. The results revealed that IBA 2500 ppm recorded the maximum rooting (65 %), number of primary roots (5.00), length (28.43 cm) and diameter (3.25 mm) of primary roots, fresh (3.67 g) and dry weight (2.59 g) of roots, length of main shoot (134.14cm), diameter of main shoot (8.18 mm), fresh (30.40 g) and dry weight (22.60 g) of shoots in cuttings of Merton 793. However, the PGPR strains RG (1)3 – Bacillus sp., B6 – Bacillus licheniformis and R3 (3) – Sirretia sp. in combination with IBA 1000 ppm showed improvement in rooting of cuttings to the extent of 10, 15 and 5 per cent rooting, respectively and growth of the rooted plants. IBA at 2500 ppm resulted better rooting and growth of rooted plants. Hence, this treatment is suggested for commercial propagation of apple clonal rootstock Merton 793 through cuttings.

scholarly journals A novel filamentous Bacillus sp., strain NAF001, forming endospores and budding cells The GenBank/EMBL/DDBJ accession number for the 16S rRNA sequence reported in this paper is AB049195.

Microbiology ◽  
2001 ◽  
Vol 147 (6) ◽  
pp. 1415-1423 ◽  
Author(s):  
Vasudevan P Ajithkumar ◽  
Bindu Ajithkumar ◽  
Koji Mori ◽  
Kazuhiro Takamizawa ◽  
Ryozo Iriye ◽  
...  
Keyword(s):  
16S Rrna ◽  
Bacillus Sp ◽  
Accession Number ◽  
Rrna Sequence ◽  
16S Rrna Sequence

scholarly journals Effect of Indigenous Pseudomonas sp. and Bacillus sp. Strains on Yield and Main Chemical Growth Parameters of Radicchio

2018 ◽  
Vol 67 (1) ◽  
pp. 20-26
Author(s):  
Aleksandra Stanojković-Sebić ◽  
Radmila Pivić ◽  
Zoran Dinić ◽  
Renata Iličić ◽  
Dragana Latković ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Aerial Parts ◽  
Dry Biomass ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Calcium And Magnesium

Summary Pseudomonas sp. and Bacillus sp. belong to plant growth promoting rhizobacteria which are able to colonize the plants roots and stimulate growth. In this study, the effect of two indigenous plant growth promoting rhizobacterial strains Pseudomonas sp. Q4 and Bacillus sp. Q10 and their mixture (mix Q4+Q10) on content of the main chemical growth parameters (nitrogen, phosphorus, potassium, calcium and magnesium) and the yield of dry biomass of radicchio (Cichorium spp. var. rossa di treviso) aerial parts and root, was investigated. The study was carried out with stagnosol type of soil in pot experiments under semi-controlled conditions in the Institute of Soil Science (Belgrade), in the period from July to October in 2013. Phosphorus was determined by spectrophotometer, potassium - by flame emission photometry and total nitrogen and carbon - using elemental CNS analyzer, while calcium and magnesium were determined by AAS. The data on yield of both aerial parts and root dry biomass of radicchio showed that its treatment with Q4 and Q10 strains, as well as with their mixture, caused noticeably increase in this parameter in relation to the control, whereby the strain Q4 was more effective for aerial parts, while mix Q4+Q10 - for roots. The obtained data on the studied chemical parameters of radicchio root and aerial parts were in total accordance with their yield. Concluding, studied strains have a potential in promoting the biomass yield and main chemical growth parameters of both aerial parts and root of radicchio.

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