scholarly journals Micropropagation of Alibertia edulis Rich

2008 ◽  
Vol 51 (6) ◽  
pp. 1103-1114 ◽  
Author(s):  
Fernanda Augusta Boato da Silva ◽  
Luiz Alfredo Rodrigues Pereira ◽  
Conceição Eneida dos Santos Silveira
Keyword(s):  
Butyric Acid ◽  
Growth Regulators ◽  
Activated Charcoal ◽  
Shoot Proliferation ◽  
Shoot Elongation ◽  
Optimum Concentration ◽  
Ms Medium ◽  
Brazilian Cerrado ◽  
Positive Effect

The aim of this work was to develop a micropropagation protocol for Alibertia edulis, a medicinal Brazilian Cerrado plant. A. edulis seeds were sterilized and germinated in water-agar. Fifteen millimeter long explants from the seedlings cultivated for 50 days were used for the multiplication in three consecutive subcultures. The cytokinin benzyladenine (BA) alone or combined with indole-3-butyric acid (IBA) was added to ¼ MS medium. The treatments containing combinations of the BA + IBA induced more shoot proliferation than the BA alone, with the optimum concentration of 0.5 mg.L-1 for both growth regulators. A. edulis shoots elongated easily and the presence of growth regulators did have a positive effect on the shoot elongation. Consecutive subcultures also had a positive effect on both sprouting and shoot elongation. Activated charcoal was essential for rooting, and all rooted plants were able to acclimatize.

scholarly journals THE EFFICIENCY OF SHOOT AND PLANTLET FORMATION OF Cephaelis ipecacuanha AFTER THREE SUBCULTURES IN VITRO

1994 ◽  
Vol 24 (3) ◽  
pp. 523-526 ◽  
Author(s):  
Osmar Alves Lameira ◽  
Marly Pedroso da Costa ◽  
José Eduardo Brasil Pereira Pinto
Keyword(s):  
Gibberellic Acid ◽  
Butyric Acid ◽  
Growth Regulators ◽  
Activated Charcoal ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Plantlet Formation ◽  
Cephaelis Ipecacuanha

Multiple adventitious shoot formed from internodal segments of Cephaelis ipecacuanha cultured 25 days on Gamborg basal medium (GAMBORG et al., 1968) supplemented with 6.66mM 6-benzylaminopurine there was a maximum of nine shoots per segment and an average of five shoots per segment formed. The presence of gibberellic acid in the subculture media promoted shoot elongation in all treatments. The shoots attained 3cm in height and rooting of 100% after 35 days of culturing upon Murashige and Skoog's basal medium (MS), added with 4.92mM indole-3-butyric acid, 0.87m gibberellic acid and 0.1% activated charcoal. Further growth was accelerated after the transfer to 1/2 MS without growth regulators. Rooted plantlets transferred to potting soil could be successfully established.

scholarly journals Micropropagation of Marsdenia brunoniana Wight & Arn. - A rare antidiabetic plant

2010 ◽  
Vol 20 (1) ◽  
pp. 7-12 ◽  
Author(s):  
A. Ugraiah ◽  
S. Karuppusamy ◽  
T. Pullaiah
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Growth Regulators ◽  
Plant Tissue ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Ms Medium ◽  
Plant Micropropagation ◽  
Number Of Shoots

Shoot multiplication of M. brunoniana Wight & Arn. was achieved from the nodal explants of mature plants using MS with different concentrations and combina-tions of growth regulators. Maximum explant response and highest number of shoots per explant was obtained on MS medium fortified with 1.0 mg/l BAP. The highest degree of shoot proliferation was found to be 90%. The combination of BAP and Kn was also found to be effective for regeneration. The regenerated shoots were successfully rooted on MS supplemented with 0.5 mg/l NAA, after sequential hardening; survival rate was 90%.  Key words: Marsdenia brunoniana, Medinal plant, Micropropagation, Conservation D. O.I. 10.3329/ptcb.v20i1.5958 Plant Tissue Cult. & Biotech. 20(1): 7-12, 2010 (June)

scholarly journals Micropropagation of the Rare Lakeside Daisy (Hymenoxys acaulis var. glabra)

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 200-201 ◽  
Author(s):  
James R. Ault
Keyword(s):  
Overall Survival ◽  
Butyric Acid ◽  
Potassium Salt ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Stem Segment ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
Shoot Initiation

Shoot tip and stem segment explants collected from greenhouse-maintained plants of Hymenoxys acaulis var. glabra were cultured in vitro for shoot initiation on a Murashige and Skoog (MS) medium supplemented with 30 g·L-1 sucrose, 2.5 μm BA, and 7 g·L-1 agar at a pH of 5.7. Unbranched shoot explants were subcultured to MS medium with 0.0, 0.5, 1, 2, 4 or 8 μm BA for shoot proliferation. A maximum of 10.3 shoots per explant was produced on the medium with 2.0 μm BA. Nonrooted shoots were subcultured to MS medium with 0.0, 0.5, 2, or 8 μm K-IBA for rooting. Maximum rooting was 90% on MS medium with 0.5 μm K-IBA. Rooted shoots were greenhouse-acclimatized for 10 days. Overall survival was 75%. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

scholarly journals In-vitro Conservation of Bacopa monniera (L.) Wettst.: A Memory Booster Plant

Our Nature ◽  
1970 ◽  
Vol 8 (1) ◽  
pp. 40-47 ◽  
Author(s):  
J. Prabha ◽  
U. Rani ◽  
A. Sen ◽  
R.N. Verma ◽  
A. Batra
Keyword(s):  
Growth Regulators ◽  
High Frequency ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Nodal Segment ◽  
Bud Break ◽  
Ms Medium ◽  
Bacopa Monniera ◽  
In Vitro Shoot Regeneration

A mass in-vitro propagation system of Bacopa monniera (L.) Wettst., a traditional medicinal herb, has been developed. Shoot proliferation and growth were greatly influenced by the months of the year during which the explant were collected. High frequency bud break coupled with maximum number of shoots through nodal segment were found in the month of June and August. Of the different growth regulators tried, for in vitro shoot regeneration, MS medium with BAP (1.0 mg/l) induced maximum number of proliferative shoots (65.00±1.453). Multiplication and elongation of the shoots were obtained after regular sub culturing on the same medium after 15-21 days. Rooting from basal end of the shoots occurred on MS medium with the addition of IBA (0.5 mg/l). After a hardening phase of 4 weeks, almost 95% transplantation was success in the field.DOI: 10.3126/on.v8i1.4311

scholarly journals (281) Micropropagation of Trifoliate Orange Rootstock (Poncirus trifoliate Raf.)

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1052C-1052 ◽  
Author(s):  
Abdelrahman Al-Wasel
Keyword(s):  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Trifoliate Orange ◽  
Mature Trees ◽  
Poncirus Trifoliate

In vitro propagation of trifoliate orange rootstock (Poncirus trifoliate Raf.) was achieved using axillary buds taken from new flushes of mature trees and then cultured on Murashige and Skoog medium (MS). The addition of growth regulators [0.5 mg·L-1 gibberellic acid (GA3) or 0.1 mg·L-1 6-benzyladenine (BA) and 0.1 mg·L-1 indol-3-butyric acid (IBA)] were necessary to promote bud breakage and shoot elongation. Shoot proliferation was induced on MS medium supplemented with various levels of BA (0.0, 0.5, 1.0, 1.5, and 2.0 mg·L-1) and α-naphthalene acetic acid (NAA) (0.0, 0.1, and 0.5 mg·L-1). Maximal shoot multiplication (9.3 shoots/explant) and elongation (2.3 cm) occurred on media containing either 1.0 mg·L-1 BA alone or with 0.1 mg·L-1 NAA. Shoots rooted better and gave high root number (7.6 roots/shoot) and long roots (5.4 cm) when cultured on a liquid MS medium provided by 0.1 mg·L-1 NAA. Rooted shoots were successfully established in soil (≥90%).

scholarly journals Impact of Plant Growth Regulators on Greek oregano Micropropagation and Antioxidant Activity

2016 ◽  
Vol 16 (2) ◽  
pp. 297-305
Author(s):  
Ely Georgieva Zayova ◽  
Maria Prokopova Geneva ◽  
Kamelia Dimitrova Miladinova-Georgieva ◽  
Marieta Georgieva Hristozkova ◽  
Ira Valkova Stancheva
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Butyric Acid ◽  
Growth Regulators ◽  
Multiple Shoots ◽  
Antioxidant Potential ◽  
Naphthalene Acetic Acid ◽  
Average Height ◽  
Ms Medium

This study highlights the development and achievements made for the micropropagation of Greek oregano (Origanum heracleoticum L. ) using stem tip explants. The shoots were cultured on Murashige and Skoog (MS) medium followed different concentrations of plant growth regulators (PGR) - 6-benzyl aminopurine, thidiazuron and zeatin at concentrations (0.5 or 1.0 mg L-1). The induction of multiple shoots from stem tip segments was the highest in MS medium supplemented with 1.0 mg L-1 zeatin. It was the most effective medium for shoot formation, which produced multiple shoots (2.7) with an average height of 3.5 cm. These shoots were transferred on half strength MS medium containing three different auxins: indole-3-butyric acid, α-naphthalene acetic acid or indole-3-acetic acid (0, 0.1 and 0.5 mg L-1) for rooting, Multiple shoots were the most efficiently rooted on ½ MS medium supplemented with 0.5 mg L-1indole-3-butyric acid. Rooted plants showed the best adaptation on pots containing peat: perlite (2: 1 v/v). The higher rates of shoots number and height per plant have a positive relationship with the production of metabolites with antioxidant potential as phenols and flavonoids as well as with ferric reducing antioxidant potential.

scholarly journals Micropropagation of Endemic and Endangered Mexican Species of Ponytail Palms

HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1481-1484 ◽  
Author(s):  
María Luisa Osorio-Rosales ◽  
Martín Mata-Rosas
Keyword(s):  
Endangered Species ◽  
Growth Regulators ◽  
Activated Charcoal ◽  
Initial Treatment ◽  
Survival Rates ◽  
Multiple Shoots ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Mexican Species

Experiments were conducted to establish an efficient protocol of micropropagation of Beaucarnea gracilis and B. recurvata two endemic and endangered Mexican species. Multiple shoots were induced by direct organogenesis from in vitro seedlings and longitudinal sections of seedlings in both species. The highest formation of shoots per explant, both B. gracilis and B. recurvata, was obtained from longitudinal sections of seedlings on Murashige and Skoog (MS) medium supplemented with 22.2 μm 6-benzylaminopurine, induced 8.2 and 11.1 shoots per explant respectively. In vitro rooting was readily achieved on MS medium with 1 g/l activated charcoal without growth regulators. According to initial treatment and depending on where the shoots come from, the rooting rates were 61% to 100% for B. gracilis, and 83% to 100% for B. recurvata. Survival rates in greenhouse conditions for both species were 80% to 100% after 3 months. These results indicate that the micropropagation of these species of Beaucarnea is technically feasible, and that in vitro culture is a useful option for the conservation and propagation of these important endangered species.

scholarly journals Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne

2022 ◽  
Author(s):  
Monika Poniewozik ◽  
Marzena Parzymies ◽  
Paweł Szot
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Activated Charcoal ◽  
Root Formation ◽  
Multiplication Rate ◽  
Ms Medium ◽  
In Vitro Morphogenesis ◽  
Half Strength ◽  
Positive Effect

Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.

scholarly journals Effect of Growth Regulators for in-vitro Mass Multiplication of Marigold

2011 ◽  
Vol 54 (3) ◽  
pp. 126-130
Author(s):  
Beena Naqvi ◽  
Yasmeen Tariq
Keyword(s):  
Gibberellic Acid ◽  
Growth Regulators ◽  
Root Development ◽  
Shoot Proliferation ◽  
Tagetes Erecta ◽  
Ms Medium ◽  
Mass Multiplication ◽  
Commercial Purpose ◽  
Tagetes Erecta L

This study describes an effective and reproducible protocol for the mass multiplication of marigold (Tagetes erecta L.) for commercial purpose. Twenty five different combinations of BAP, IAA, GA3 and AgNO3 were added to the basal MS medium to culture marigold explants. The highest mean number (4.83±0.49) and length (5.28 cm ±1.06) of healthy shoots per explant was observed in media supplemented with 2 mg/L BAP along with 2 mg/L IAA. When these shoots were sub-cultured for root development, the maximum number (17.08±2.44) and length (13.67 cm ±0.98) of roots were produced   in media supplemented with 4mg/L BAP and 2 mg/L IAA. Addition of gibberellic acid and AgNO3 did not have any significant effect on shoot proliferation and root development of marigold.

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