Somatic Embryo from Basal Leaf Segments of Vanda tricolor Lindl. var. pallida

<p class="Els-Abstract-text">Somatic embryogenesis is one of techniques in plant micropropagation. The induction of somatic embryogenesis through callus phase was done on <em>Vanda tricolor</em> Lindl. var. pallida. This study aimed to find out the effect of naphtalene acetic acid (NAA) and benzyl amino purine (BAP) in inducing somatic embryogenesis via callus on the basal leaf segments of <em>Vanda tricolor</em> Lindl. var. pallida. The half-strength of Murashige and Skoog (½ MS) medium with 1 % sucrose, incorporated with (0.02 mg · L^–1and 0.05 mg · L^–1) NAA and also 0.01 mg · L^–1 BAP were used in this experiment. The best medium for embryogenic callus formation and proliferation was 0.05 mg · L^–1 NAA in combination with 0.01 mg · L^–1 BAP. The formation of somatic embryos occurred 30 d after the calluses were cultured on to ½ MS without the addition of plant growth regulator and subsequently formed shoots.</p>

Micropropagation of Marsdenia brunoniana Wight & Arn. - A Rare Antidiabetic Plant

Shoot multiplication of Marsdenia brunoniana was obtained from the nodal explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response and highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of shoot profilieration was found to be 90%. The combination of BAP and Kn was also found to be effective for regeneration. The regenerated shoots were successfully rooted on MS supplemented with 0.5 mg/l NAA, after sequential hardening, survival rate was 90%. Key words: Marsdenia brunoniana, Antidiabetic plant, Micropropagation, Conservation D. O. I. 10.3329/ptcb.v21i1.9613 Plant Tissue Cult. & Biotech. 21(1): 89-93, 2011 (June) - Short communication

Nemesia Root Hair Response to Paper Pulp Substrate for Micropropagation

Agar substrates forin vitroculture are well adapted to plant micropropagation, but not to plant rooting and acclimatization. Conversely, paper-pulp-based substrates appear as potentially well adapted forin vitroculture and functional root production. To reinforce this hypothesis, this study comparesin vitrodevelopment of nemesia on several substrates. Strong differences between nemesia roots growing in agar or in paper-pulp substrates were evidenced through scanning electron microscopy. Roots developed in agar have shorter hairs, larger rhizodermal cells, and less organized root caps than those growing on paper pulp. In conclusion, it should be noted that in this study,in vitromicroporous substrates such as paper pulp lead to the production of similar root hairs to those found in greenhouse peat substrates. Consequently, if agar could be used for micropropagation, rooting, and plant acclimatization, enhancement could be achieved if rooting stage was performed on micro-porous substrates such as paper pulp.