Evaluation of an IBAD thin-film process as an alternative method for surface incorporation of bioceramics on dental implants: a study in dogs

Thin-film bioceramic coatings are potential alternatives to overcome the limitations provided by other commercially available coating techniques like PSHA, where variable bioceramic dissolution added to a metalloceramic weak link are process- inherent. The purpose of this investigation was to determine the overall and site specific (to 0.5 mm from implant surface) levels of osseoactivity around a thin-film (IBAD processed) coated titanium alloy implant versus a non surface modified (sand-blasted/acid etched) titanium alloy implant in a canine model. The surgical model comprised the proximal tibiae epiphyses with four implants placed in each limb remaining for 2 and 4 weeks in-vivo. 10 mg/Kg oxytetracycline was administered 48 hours prior to euthanization. The limbs were retrieved by sharp dissection, reduced to blocks, and subsequently nondecalcified processed for fluorescent microscopy. Micrographs (20x mag) were acquired around the implant perimeter and merged for overall biological response evaluations, and four micrographs (40x mag. subdivided in rectangles) were acquired along one of the implant sides for tetracycline labeled area fraction quantification. The results showed biocompatible and osseoconductive properties for the thin-film coated and uncoated titanium alloy implants. Tetracycline labeled area fraction analyses showed that the thin-film coated implants presented significantly higher overall and site specific osseoactivity levels at 2 and 4 weeks. The site specific osseoactivity values were significantly higher compared to overall values for control and thin-film coated implants at both times in-vivo. According to the results obtained in this study, thin-film coated implants enhanced biological response at the early implantation times evaluated.

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Intraosseous Temperature Change during Installation of Dental Implants with Two Different Surfaces and Different Drilling Protocols: An In Vivo Study in Sheep

Journal of Clinical Medicine ◽

10.3390/jcm8081198 ◽

2019 ◽

Vol 8 (8) ◽

pp. 1198 ◽

Cited By ~ 4

Author(s):

Michele Stocchero ◽

Yohei Jinno ◽

Marco Toia ◽

Marianne Ahmad ◽

Evaggelia Papia ◽

...

Keyword(s):

Temperature Increase ◽

Element Model ◽

Multiple Regression Model ◽

Metatarsal Bone ◽

Area Fraction ◽

Bone Area ◽

Implant Surface ◽

Bone Necrosis ◽

Bone To Implant Contact

Background: The intraosseous temperature during implant installation has never been evaluated in an in vivo controlled setup. The aims were to investigate the influence of a drilling protocol and implant surface on the intraosseous temperature during implant installation, to evaluate the influence of temperature increase on osseointegration and to calculate the heat distribution in cortical bone. Methods: Forty Brånemark implants were installed into the metatarsal bone of Finnish Dorset crossbred sheep according to two different drilling protocols (undersized/non-undersized) and two surfaces (moderately rough/turned). The intraosseous temperature was recorded, and Finite Element Model (FEM) was generated to understand the thermal behavior. Non-decalcified histology was carried out after five weeks of healing. The following osseointegration parameters were calculated: Bone-to-implant contact (BIC), Bone Area Fraction Occupancy (BAFO), and Bone Area Fraction Occupancy up to 1.5 mm (BA1.5). A multiple regression model was used to identify the influencing variables on the histomorphometric parameters. Results: The temperature was affected by the drilling protocol, while no influence was demonstrated by the implant surface. BIC was positively influenced by the undersized drilling protocol and rough surface, BAFO was negatively influenced by the temperature rise, and BA1.5 was negatively influenced by the undersized drilling protocol. FEM showed that the temperature at the implant interface might exceed the limit for bone necrosis. Conclusion: The intraosseous temperature is greatly increased by an undersized drilling protocol but not from the implant surface. The temperature increase negatively affects the bone healing in the proximity of the implant. The undersized drilling protocol for Brånemark implant systems increases the amount of bone at the interface, but it negatively impacts the bone far from the implant.

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Biological response of laser macrostructured and oxidized titanium alloy: An in vitro and in vivo study

Journal of Applied Biomaterials & Functional Materials ◽

10.5301/jabb.2011.8923 ◽

2011 ◽

Vol 9 (3) ◽

pp. 214-222

Author(s):

Maria Dolores Paz ◽

J. Iñaki Álava ◽

Leire Goikoetxea ◽

Stefano Chiussi ◽

Idoia Díaz-Güemes ◽

...

Keyword(s):

Titanium Alloy ◽

Biological Response ◽

In Vivo Study

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Histomorphometrical Comparison of Alumina-Blasted/Acid-Etched and a Thin Ion Beam Deposited Bioceramic Surfaces at Early Implantation Times. An Experimental Study in Dogs.

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.396-398.327 ◽

2008 ◽

Vol 396-398 ◽

pp. 327-330

Author(s):

Marcelo Suzuki ◽

Charles Marin ◽

Rodrigo Granato ◽

Paulo Guilherme Coelho

Keyword(s):

Thin Film ◽

Dental Implants ◽

Ion Beam ◽

Canine Model ◽

Bone Architecture ◽

Control Surface ◽

Metallic Surface ◽

Implant Surface ◽

Bone Response ◽

Bioceramic Coatings

Among surface modifications commercially available for dental implants, the incorporation of bioceramic coatings is one of the most popular. However, concerns regarding the effectiveness of the bond between the metallic surface and the coating have led to the development of thin-film Ca- and P- based bioceramic coatings. The purpose of this study was to evaluate the early bone response to a thin ion bean deposited (Test) bioceramic implant surface compared to an alumina-blasted/acid-etched (Control) surface in a canine model. Results showed that although no difference in bone-to-implant (BIC) could be noted between the two different surfaces, more organized bone architecture was present around the Test implants in 4 weeks. Based on this observation, the incorporation of a thin- film bioceramic coating positively influenced bone healing around dental implants at early times.

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Histologic and Biomechanical Evaluation of Alumina-Blasted/Acid-Etched and Resorbable Blasting Media Surfaces

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-10-00105 ◽

2012 ◽

Vol 38 (5) ◽

pp. 549-557 ◽

Cited By ~ 11

Author(s):

Estevam A. Bonfante ◽

Charles Marin ◽

Rodrigo Granato ◽

Marcelo Suzuki ◽

Jenni Hjerppe ◽

...

Keyword(s):

Canine Model ◽

Implant Surface ◽

Surface Time ◽

Biomechanical Evaluation ◽

Bone To Implant Contact ◽

Calcium And Phosphorus

This study evaluated the early biomechanical fixation and bone-to-implant contact (BIC) of an alumina-blasted/acid-etched (AB/AE) compared with an experimental resorbable blasting media (RBM) surface in a canine model. Higher texturization was observed for the RBM than for the AB/AE surface, and the presence of calcium and phosphorus was only observed for the RBM surface. Time in vivo and implant surface did not influence torque. For both surfaces, BIC significantly increased from 2 to 4 weeks.

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In Vivo Studies of a Nanostructured Titanium Alloy Knee Plate and Pin

MRS Proceedings ◽

10.1557/opl.2011.1523 ◽

2011 ◽

Vol 1349 ◽

Author(s):

Julia C. Mirza Rosca ◽

Eladio D. Herrera Santana ◽

C. Vasilescu ◽

José A. García Llorente

Keyword(s):

Titanium Alloy ◽

Histological Examination ◽

Orthopedic Implants ◽

In Vivo Studies ◽

Free Access ◽

Implant Surface ◽

Total Joint Replacements ◽

Titanium Alloy Ti6al4v ◽

Mini Pigs

ABSTRACTTitanium alloys are among the most used metallic biomaterials, particularly for orthopedic applications. Ever since the pioneer titanium alloy (Ti6Al4V) has been used as biomaterial, lack of biocompatibility has been extensively reported and propelled research on improved materials with appropriate mechanical behavior and adequate biocompatibility. Studies have indicated that vanadium produces oxides harmful to the human body; in order to replace vanadium containing Ti alloys, Ti-6Al-7Nb was developed. Today this alloy is the preferred choice for cementless total joint replacements. It is very important to produce a nanostructured bioactive metal implant with appropriate mechanical properties and we applied a chemical and thermal treatment that converts the surface of titanium alloy into bioactive surface. Therefore, bioactive Ti6Al7Nb might represent an alternative for advanced orthopedic implants under load-bearing conditions.Eleven mini-pigs weighting around 50 kg, with free access to food pellets and water, were the experimental animals for this study. Ten of these pigs (one is the control) were anesthetized and after shaving, disinfection and draping, a straight 3 cm incision was made and the implants (plate and pin) were implanted into the epiphyses of the tibiae. Surgical procedures were performed bilaterally. At 6 months after implantation, the mini-pigs were sacrificed.After sacrifice, the segments of the proximal tibia epiphyses containing the implanted plates and pins were cut of, fixed in phosphate-buffered formalin and dehydrated in serial concentrations of ethanol after which they were embedded in polyester resin and then cutted and grounded to a thickness of 75-100 μm. With these samples a lot of observations were made: Scanning Electron Microscopy observations, histological examination at implant surface and histological examination of the bone-implant surface and SEM-EDX examinations were also made.All the results revealed that the plates and pins are in direct contact with newly formed bone without any intervening soft tissue layer. We regard osteoinductive ability of nanostructured Ti6Al7Nb as one of the advantages of this implant in consideration for clinical applications.

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Comparison of High Purity Factor IX Concentrates and a Prothrombin Complex Concentrate in a Canine Model of Thrombogenicity

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646468 ◽

1991 ◽

Vol 66 (05) ◽

pp. 609-613 ◽

Cited By ~ 14

Author(s):

I R MacGregor ◽

J M Ferguson ◽

L F McLaughlin ◽

T Burnouf ◽

C V Prowse

Keyword(s):

High Purity ◽

Time Course ◽

Prothrombin Complex Concentrate ◽

Degradation Products ◽

Canine Model ◽

Factor Ix ◽

In Vitro Tests ◽

Albumin Infusion

SummaryA non-stasis canine model of thrombogenicity has been used to evaluate batches of high purity factor IX concentrates from 4 manufacturers and a conventional prothrombin complex concentrate (PCC). Platelets, activated partial thromboplastin time (APTT), fibrinogen, fibrin(ogen) degradation products and fibrinopeptide A (FPA) were monitored before and after infusion of concentrate. Changes in FPA were found to be the most sensitive and reproducible indicator of thrombogenicity after infusion of batches of the PCC at doses of between 60 and 180 IU/kg, with a dose related delayed increase in FPA occurring. Total FPA generated after 100-120 IU/kg of 3 batches of PCC over the 3 h time course was 9-12 times that generated after albumin infusion. In contrast the amounts of FPA generated after 200 IU/kg of the 4 high purity factor IX products were in all cases similar to albumin infusion. It was noted that some batches of high purity concentrates had short NAPTTs indicating that current in vitro tests for potential thrombogenicity may be misleading in predicting the effects of these concentrates in vivo.

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The Development of Homologous (Canine/Anti-Canine) Antibodies in Dogs with Haemophilia A (Factor VIII Deficiency): A Ten-Year Longitudinal Study

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651541 ◽

1993 ◽

Vol 69 (01) ◽

pp. 021-024 ◽

Cited By ~ 20

Author(s):

Shawn Tinlin ◽

Sandra Webster ◽

Alan R Giles

Keyword(s):

Factor Viii ◽

Canine Model ◽

Significant Inhibition ◽

Human Condition ◽

Haemophilia A ◽

Variable Expression ◽

The Family ◽

Over Time

SummaryThe development of inhibitors to factor VIII in patients with haemophilia A remains as a serious complication of replacement therapy. An apparently analogous condition has been described in a canine model of haemophilia A (Giles et al., Blood 1984; 63:451). These animals and their relatives have now been followed for 10 years. The observation that the propensity for inhibitor development was not related to the ancestral factor VIII gene has been confirmed by the demonstration of vertical transmission through three generations of the segment of the family related to a normal (non-carrier) female that was introduced for breeding purposes. Haemophilic animals unrelated to this animal have not developed functionally significant factor VIII inhibitors despite intensive factor VIII replacement. Two animals have shown occasional laboratory evidence of factor VIII inhibition but this has not been translated into clinical significant inhibition in vivo as assessed by clinical response and F.VIII recovery and survival characteristics. Substantial heterogeneity of inhibitor expression both in vitro and in vivo has been observed between animals and in individual animals over time. Spontaneous loss of inhibitors has been observed without any therapies designed to induce tolerance, etc., being instituted. There is also phenotypic evidence of polyclonality of the immune response with variable expression over time in a given animal. These observations may have relevance to the human condition both in determining the pathogenetic factors involved in this condition and in highlighting the heterogeneity of its expression which suggests the need for caution in the interpretation of the outcome of interventions designed to modulate inhibitor activity.

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