Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom

20-Hydroxyeicosatetraenoic acid (20-HETE), a cytochrome P-450 metabolite of arachidonic acid, is a vasoconstrictor in the systemic circulation and a vasodilator in the adult pulmonary circulation. Little is known about the vasoactive properties of 20-HETE in the newborn pulmonary circulation. The objectives of this study were to determine the vascular effects of 20-HETE and to explore the signaling mechanism(s) that mediate these effects in newborn pulmonary resistance-level arteries (PRA). Our findings demonstrate that, in contrast to the adult pulmonary circulation where 20-HETE mediates vasodilation, it causes constriction in newborn PRA at resting tone. Furthermore, inhibition of cyclooxygenase (COX) with indomethacin augments 20-HETE-induced constriction. The enhanced constrictor response to 20-HETE under conditions of COX inhibition is abolished in endothelium-disrupted PRA, suggesting that 20-HETE either stimulates endothelium-derived COX to release a counteracting vasodilator or is rapidly metabolized by COX to a less potent vasoconstrictor. 20-HETE-induced constriction is significantly inhibited by blocking calcium-dependent K+ (KCa) channels and the thromboxane-PGH2 receptor. Altogether, our data indicate that the vascular actions of 20-HETE are partially mediated via the activation of KCa channels and are significantly modulated by interactions with the COX-prostaglandin pathway.

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Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen from Polybia paulista Venom

Toxins ◽

10.3390/toxins12060379 ◽

2020 ◽

Vol 12 (6) ◽

pp. 379

Author(s):

Luís Gustavo Romani Fernandes ◽

Amilcar Perez-Riverol ◽

Murilo Luiz Bazon ◽

Débora Moitinho Abram ◽

Márcia Regina Brochetto-Braga ◽

...

Keyword(s):

Immune Response ◽

T Cells ◽

T Cell ◽

Cd4 T Cells ◽

Cell Subset ◽

Cell Immune Response ◽

Venom Immunotherapy ◽

Phospholipase A1 ◽

Wasp Venom ◽

Polybia Paulista

Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from Polybia paulista wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated with Polybia paulista venom extract and the proliferation of CD8+ and CD4+ T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4+ T cells, but not of CD8+ T cells. The cytokine pattern showed a high concentration of IFN-γ and IL-6, and no significant differences to IL-4, IL-1β and TGF-β1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4+CD25+FoxP3+ and CD4+CD25-FoxP3+ Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4+ T cell subset, suggesting its potential use in wasp venom immunotherapy.

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Postsynaptic effects of Polybia paulista wasp venom on skeletal muscle

Toxicon ◽

10.1016/s0041-0101(00)80135-x ◽

2000 ◽

Vol 38 (4) ◽

pp. 580-581

Keyword(s):

Skeletal Muscle ◽

Wasp Venom ◽

Polybia Paulista

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Myotoxic effects of mastoparan from Polybia paulista (Hymenoptera, Epiponini) wasp venom in mice skeletal muscle

Toxicon ◽

10.1016/j.toxicon.2007.05.003 ◽

2007 ◽

Vol 50 (5) ◽

pp. 589-599 ◽

Cited By ~ 18

Author(s):

Thalita Rocha ◽

Bibiana Monson de Souza ◽

Mario Sérgio Palma ◽

Maria Alice da Cruz-Höfling

Keyword(s):

Skeletal Muscle ◽

Wasp Venom ◽

Polybia Paulista

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B-cell linear epitopes mapping of antigen-5 allergen from Polybia paulista wasp venom

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2014.07.006 ◽

2015 ◽

Vol 135 (1) ◽

pp. 264-267.e8 ◽

Cited By ~ 13

Author(s):

José Roberto Aparecido dos Santos-Pinto ◽

Lucilene Delazari dos Santos ◽

Helen Andrade Arcuri ◽

Anally Ribeiro da Silva Menegasso ◽

Paloma Napoleão Pêgo ◽

...

Keyword(s):

B Cell ◽

Wasp Venom ◽

Polybia Paulista ◽

Linear Epitopes ◽

Antigen 5

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Trypanocidal activity of mastoparan from Polybia paulista wasp venom by interaction with TcGAPDH

Toxicon ◽

10.1016/j.toxicon.2017.08.002 ◽

2017 ◽

Vol 137 ◽

pp. 168-172 ◽

Cited By ~ 10

Author(s):

Juliana Freire Chagas Vinhote ◽

Dânya Bandeira Lima ◽

Ramon Róseo Paula Pessoa Bezerra de Menezes ◽

Clarissa Perdigão Mello ◽

Bibiana Monson de Souza ◽

...

Keyword(s):

Wasp Venom ◽

Trypanocidal Activity ◽

Polybia Paulista

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Improved production of the recombinant phospholipase A1 from Polybia paulista wasp venom expressed in bacterial cells for use in routine diagnostics

3 Biotech ◽

10.1007/s13205-020-02202-8 ◽

2020 ◽

Vol 10 (5) ◽

Author(s):

Amilcar Perez-Riverol ◽

Alexis Musacchio-Lasa ◽

Luis Gustavo Romani Fernandes ◽

Jose Roberto Aparecido dos Santos-Pinto ◽

Franciele Grego Esteves ◽

...

Keyword(s):

Bacterial Cells ◽

Phospholipase A1 ◽

Wasp Venom ◽

Routine Diagnostics ◽

Polybia Paulista

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Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050287 ◽

1974 ◽

Vol 32 ◽

pp. 54-55

Author(s):

S. Phyllis Steamer ◽

Rosemarie L. Devine

Keyword(s):

Structural Changes ◽

Radiation Treatment ◽

Arterial Stenosis ◽

Ultrastructural Changes ◽

Vascular Effects ◽

Microvascular Changes ◽

Surrounding Connective Tissue ◽

Fission Neutrons ◽

Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

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