Myotoxic effects of mastoparan from Polybia paulista (Hymenoptera, Epiponini) wasp venom in mice skeletal muscle

Toxicon ◽  
2007 ◽  
Vol 50 (5) ◽  
pp. 589-599 ◽  
Author(s):  
Thalita Rocha ◽  
Bibiana Monson de Souza ◽  
Mario Sérgio Palma ◽  
Maria Alice da Cruz-Höfling
Keyword(s):  
Skeletal Muscle ◽  
Wasp Venom ◽  
Polybia Paulista

scholarly journals Heterologous Expression, Purification and Immunoreactivity of the Antigen 5 from Polybia paulista Wasp Venom

Toxins ◽  
2017 ◽  
Vol 9 (9) ◽  
pp. 259 ◽  
Author(s):  
Murilo Bazon ◽  
Amilcar Perez-Riverol ◽  
José dos Santos-Pinto ◽  
Luis Fernandes ◽  
Alexis Lasa ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Wasp Venom ◽  
Polybia Paulista ◽  
Antigen 5

scholarly journals Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom

2011 ◽  
Vol 17 (2) ◽  
pp. 199-208 ◽  
Author(s):  
JFC Vinhote ◽  
AFC Torres ◽  
RT Dantas ◽  
TP Praciano ◽  
RRPPB Menezes ◽  
...  
Keyword(s):  
Wasp Venom ◽  
Vascular Effects ◽  
Calcium Dependent ◽  
Polybia Paulista

Revisiting Polybia paulista wasp venom using shotgun proteomics – Insights into the N-linked glycosylated venom proteins

2019 ◽  
Vol 200 ◽  
pp. 60-73 ◽  
Author(s):  
Caroline Lacerra de Souza ◽  
José Roberto Aparecido dos Santos-Pinto ◽  
Franciele Grego Esteves ◽  
Amilcar Perez-Riverol ◽  
Luís Gustavo Romani Fernandes ◽  
...  
Keyword(s):  
Shotgun Proteomics ◽  
Wasp Venom ◽  
Polybia Paulista ◽  
Venom Proteins

scholarly journals Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen from Polybia paulista Venom

Toxins ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 379
Author(s):  
Luís Gustavo Romani Fernandes ◽  
Amilcar Perez-Riverol ◽  
Murilo Luiz Bazon ◽  
Débora Moitinho Abram ◽  
Márcia Regina Brochetto-Braga ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cells ◽  
T Cell ◽  
Cd4 T Cells ◽  
Cell Subset ◽  
Cell Immune Response ◽  
Venom Immunotherapy ◽  
Phospholipase A1 ◽  
Wasp Venom ◽  
Polybia Paulista

Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from Polybia paulista wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated with Polybia paulista venom extract and the proliferation of CD8+ and CD4+ T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4+ T cells, but not of CD8+ T cells. The cytokine pattern showed a high concentration of IFN-γ and IL-6, and no significant differences to IL-4, IL-1β and TGF-β1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4+CD25+FoxP3+ and CD4+CD25-FoxP3+ Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4+ T cell subset, suggesting its potential use in wasp venom immunotherapy.

B-cell linear epitopes mapping of antigen-5 allergen from Polybia paulista wasp venom

2015 ◽  
Vol 135 (1) ◽  
pp. 264-267.e8 ◽  
Author(s):  
José Roberto Aparecido dos Santos-Pinto ◽  
Lucilene Delazari dos Santos ◽  
Helen Andrade Arcuri ◽  
Anally Ribeiro da Silva Menegasso ◽  
Paloma Napoleão Pêgo ◽  
...  
Keyword(s):  
B Cell ◽  
Wasp Venom ◽  
Polybia Paulista ◽  
Linear Epitopes ◽  
Antigen 5

scholarly journals Trypanocidal activity of mastoparan from Polybia paulista wasp venom by interaction with TcGAPDH

Toxicon ◽  
2017 ◽  
Vol 137 ◽  
pp. 168-172 ◽  
Author(s):  
Juliana Freire Chagas Vinhote ◽  
Dânya Bandeira Lima ◽  
Ramon Róseo Paula Pessoa Bezerra de Menezes ◽  
Clarissa Perdigão Mello ◽  
Bibiana Monson de Souza ◽  
...  
Keyword(s):  
Wasp Venom ◽  
Trypanocidal Activity ◽  
Polybia Paulista

scholarly journals Improved production of the recombinant phospholipase A1 from Polybia paulista wasp venom expressed in bacterial cells for use in routine diagnostics

3 Biotech ◽  
2020 ◽  
Vol 10 (5) ◽  
Author(s):  
Amilcar Perez-Riverol ◽  
Alexis Musacchio-Lasa ◽  
Luis Gustavo Romani Fernandes ◽  
Jose Roberto Aparecido dos Santos-Pinto ◽  
Franciele Grego Esteves ◽  
...  
Keyword(s):  
Bacterial Cells ◽  
Phospholipase A1 ◽  
Wasp Venom ◽  
Routine Diagnostics ◽  
Polybia Paulista

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

1974 ◽  
Vol 32 ◽  
pp. 148-149
Author(s):  
D. E. Philpott ◽  
A. Takahashi
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Salivary Gland ◽  
Carotid Artery ◽  
Basement Membrane ◽  
Coronary Vessels ◽  
Ruthenium Red ◽  
E Coli ◽  
Old Rats ◽  
The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

Sign in / Sign up

Export Citation Format

Share Document