Heterologous Expression, Purification and Immunoreactivity of the Antigen 5 from Polybia paulista Wasp Venom

The social wasp Polybia paulista (Hymenoptera, Vespidae) is highly aggressive, being responsible for many medical occurrences. One of the most allergenic components of this venom is Antigen 5 (Poly p 5). The possible modulation of the in vitro immune response induced by antigen 5 from P. paulista venom, expressed recombinantly (rPoly p 5), on BALB/c mice peritoneal macrophages, activated or not with LPS, was assessed. Here, we analyzed cell viability changes, expression of the phosphorylated form of p65 NF-κB subunit, nitric oxide (NO), proinflammatory cytokines production, and co-stimulatory molecules (CD80, CD86). The results suggest that rPoly p 5 does not affect NO production nor the expression of co-stimulatory molecules in mouse peritoneal macrophages. On the other hand, rPoly p 5 induced an increase in IL-1β production in non-activated macrophages and a reduction in the production of TNF-α and MCP-1 cytokines in activated macrophages. rPoly p 5 decreased the in vitro production of the phosphorylated p65 NF-κB subunit in non-activated macrophages. These findings suggest an essential role of this allergen in the polarization of functional M2 macrophage phenotypes, when analyzed in previously activated macrophages. Further investigations, mainly in in vivo studies, should be conducted to elucidate Polybia paulista Ag5 biological role in the macrophage functional profile modulation.

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A novel cDNA from Drosophila encoding a protein with similarity to mammalian cysteine-rich secretory proteins, wasp venom antigen 5, and plant group 1 pathogenesis-related proteins

Gene ◽

10.1016/s0378-1119(97)00010-3 ◽

1997 ◽

Vol 191 (2) ◽

pp. 135-141 ◽

Cited By ~ 38

Author(s):

Michael C Schreiber ◽

J.Colleen Karlo ◽

Gae E Kovalick

Keyword(s):

Secretory Proteins ◽

Wasp Venom ◽

Pathogenesis Related Proteins ◽

Plant Group ◽

Pathogenesis Related ◽

Related Proteins ◽

Antigen 5 ◽

Group 1

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Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom

Journal of Venomous Animals and Toxins including Tropical Diseases ◽

10.1590/s1678-91992011000200011 ◽

2011 ◽

Vol 17 (2) ◽

pp. 199-208 ◽

Cited By ~ 5

Author(s):

JFC Vinhote ◽

AFC Torres ◽

RT Dantas ◽

TP Praciano ◽

RRPPB Menezes ◽

...

Keyword(s):

Wasp Venom ◽

Vascular Effects ◽

Calcium Dependent ◽

Polybia Paulista

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Using Proteomic Strategies for Sequencing and Post-Translational Modifications Assignment of Antigen-5, a Major Allergen from the Venom of the Social Wasp Polybia paulista

Journal of Proteome Research ◽

10.1021/pr4008927 ◽

2013 ◽

Vol 13 (2) ◽

pp. 855-865 ◽

Cited By ~ 21

Author(s):

José Roberto Aparecido dos Santos-Pinto ◽

Lucilene Delazari dos Santos ◽

Helen Andrade Arcuri ◽

Fábio Morato Castro ◽

Jorge Elias Kalil ◽

...

Keyword(s):

Social Wasp ◽

Major Allergen ◽

Post Translational Modifications ◽

The Social ◽

Polybia Paulista ◽

Antigen 5

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Revisiting Polybia paulista wasp venom using shotgun proteomics – Insights into the N-linked glycosylated venom proteins

Journal of Proteomics ◽

10.1016/j.jprot.2019.03.012 ◽

2019 ◽

Vol 200 ◽

pp. 60-73 ◽

Cited By ~ 1

Author(s):

Caroline Lacerra de Souza ◽

José Roberto Aparecido dos Santos-Pinto ◽

Franciele Grego Esteves ◽

Amilcar Perez-Riverol ◽

Luís Gustavo Romani Fernandes ◽

...

Keyword(s):

Shotgun Proteomics ◽

Wasp Venom ◽

Polybia Paulista ◽

Venom Proteins

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Wasp Venom Biochemical Components and Their Potential in Biological Applications and Nanotechnological Interventions

Toxins ◽

10.3390/toxins13030206 ◽

2021 ◽

Vol 13 (3) ◽

pp. 206

Author(s):

Aida Abd El-Wahed ◽

Nermeen Yosri ◽

Hanem H. Sakr ◽

Ming Du ◽

Ahmed F. M. Algethami ◽

...

Keyword(s):

Solid Phase ◽

Solid Phase Peptide Synthesis ◽

Biological Applications ◽

Bioactive Constituents ◽

Wasp Venom ◽

New Agents ◽

Current Review ◽

Medicinal Value ◽

Antigen 5 ◽

Different Parts

Wasps, members of the order Hymenoptera, are distributed in different parts of the world, including Brazil, Thailand, Japan, Korea, and Argentina. The lifestyles of the wasps are solitary and social. Social wasps use venom as a defensive measure to protect their colonies, whereas solitary wasps use their venom to capture prey. Chemically, wasp venom possesses a wide variety of enzymes, proteins, peptides, volatile compounds, and bioactive constituents, which include phospholipase A2, antigen 5, mastoparan, and decoralin. The bioactive constituents have anticancer, antimicrobial, and anti-inflammatory effects. However, the limited quantities of wasp venom and the scarcity of advanced strategies for the synthesis of wasp venom’s bioactive compounds remain a challenge facing the effective usage of wasp venom. Solid-phase peptide synthesis is currently used to prepare wasp venom peptides and their analogs such as mastoparan, anoplin, decoralin, polybia-CP, and polydim-I. The goal of the current review is to highlight the medicinal value of the wasp venom compounds, as well as limitations and possibilities. Wasp venom could be a potential and novel natural source to develop innovative pharmaceuticals and new agents for drug discovery.

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Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen from Polybia paulista Venom

Toxins ◽

10.3390/toxins12060379 ◽

2020 ◽

Vol 12 (6) ◽

pp. 379

Author(s):

Luís Gustavo Romani Fernandes ◽

Amilcar Perez-Riverol ◽

Murilo Luiz Bazon ◽

Débora Moitinho Abram ◽

Márcia Regina Brochetto-Braga ◽

...

Keyword(s):

Immune Response ◽

T Cells ◽

T Cell ◽

Cd4 T Cells ◽

Cell Subset ◽

Cell Immune Response ◽

Venom Immunotherapy ◽

Phospholipase A1 ◽

Wasp Venom ◽

Polybia Paulista

Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from Polybia paulista wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated with Polybia paulista venom extract and the proliferation of CD8+ and CD4+ T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4+ T cells, but not of CD8+ T cells. The cytokine pattern showed a high concentration of IFN-γ and IL-6, and no significant differences to IL-4, IL-1β and TGF-β1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4+CD25+FoxP3+ and CD4+CD25-FoxP3+ Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4+ T cell subset, suggesting its potential use in wasp venom immunotherapy.

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