scholarly journals Detailed morphological description of Habronema clarki Foster & Chitwood, 1937, a nematode parasite of capybaras Hydrochoerus hydrochaeris (Linnaeus, 1766) in Brazil

2014 ◽  
Vol 23 (2) ◽  
pp. 251-254
Author(s):  
Estevam Guilherme Lux Hoppe ◽  
José Hairton Tebaldi ◽  
Daniel Fontana Ferreira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nematode Parasite ◽  
Morphological Data ◽  
Valid Species ◽  
Morphological Description ◽  
Hydrochoerus Hydrochaeris ◽  
Scanning Electron

The genus Habronema has four valid species, of which only two are properly known. The present study aimed to describe in detail the morphology of Habronema clarki through optical and scanning electron microscopy analyses. Our results showed that the labial morphology of this parasite is closer to H. muscae than to H. microstoma. Even so, the characteristic pseudolabia and the slightly convex border of the dorsal and ventral lips are sufficient to distinguish these nematodes. Additional morphological data are presented, thus contributing to the knowledge on this little known nematode. In addition, this study provides new locality records for this species.

Redescription of Diplogasteroides nasuensis Takaki, 1941 and D. magnus Völk, 1950 (Nematoda: Diplogastrina) associated with Scarabaeidae (Coleoptera)

Nematology ◽  
2001 ◽  
Vol 3 (8) ◽  
pp. 817-832 ◽  
Author(s):  
Walter Sudhaus ◽  
Karin Kiontke ◽  
Albrecht Manegold
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Broad Sense ◽  
Morphological Description ◽  
Scanning Electron

AbstractA detailed morphological description is presented of Diplogasteroides nasuensis Takaki, 1941 and D. magnus Völk, 1950, using light and scanning electron microscopy. Neoaplectana melolonthae Weiser, 1958 and Diplogasteroides (Rhabdontolaimus) berwigi Rühm, 1959 are synonymised with D. nasuensis. This gonochoristic species is distinguished from the hermaphroditic D. magnus by the dorsal metastomal decoration which consists of bristle-like protrusions instead of a more or less uniform tooth. The spermatocytes in D. nasuensis are twice as big as those in hermaphrodites of D. magnus. Dauer juveniles of D. nasuensis are considerably bigger than those of D. magnus. Dauer juveniles of both species are present on wood cockchafer (Melolontha hippocastani) larvae and adults but, on adult beetles, D. nasuensis dauer juveniles enter the genital pouch, whereas those of D. magnus are found under the hind coxae. In Germany, D. nasuensis is found only on M. hippocastani. D. magnus dauer juveniles are also found on a variety of other Scarabaeidae. Further small differences distinguish the two species. The genus Diplogasteroides is proposed to be taken in a broad sense. Several genera names are synonymised with Diplogasteroides.

New data on the morphology of Comephoronema oschmarini (Nematoda, Cystidicolidae), a little-known gastrointestinal parasite of Lota lota (Teleostei) in Palaearctic Eurasia

2007 ◽  
Vol 52 (2) ◽  
Author(s):  
František Moravec ◽  
Vladimíra Hanzelová ◽  
Daniel Gerdeaux
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Features ◽  
Valid Species ◽  
Lake Geneva ◽  
Gastrointestinal Parasite ◽  
Intensity Of Infection ◽  
Lota Lota ◽  
Scanning Electron

AbstractThe nematode Comephoronema oschmarini Trofimenko, 1974 (Cystidicolidae) was found in the stomach of the burbot Lota lota (Linnaeus) of Lake Geneva, Savoy, France, collected on 23 March 2000 [prevalence 60% (3/5); intensity of infection 2–66 (mean 35)]. This material made it possible to study in detail the morphology of this so far little-known parasite, using both light and scanning electron microscopy (the latter not previously used for this species). The SEM examination showed taxonomically important morphological features, not previously reported, including the presence of distinct terminal pseudolabial projections (protuberances), four submedian labia, four bilobed submedian sublabia, and only four cephalic papillae. Deirids were found to be bifurcated. A description of the true arrangement of the two last pairs of minute caudal papillae and phasmids in the male is given. Species of Compehoronema Layman, 1933 are considered to be closely related to those of Ascarophis van Beneden, 1871. A key to valid species of Comephoronema is provided.

scholarly journals Scanning electron microscopy image representativeness: morphological data on nanoparticles

2016 ◽  
Vol 265 (1) ◽  
pp. 34-50 ◽  
Author(s):  
KATARZYNA ODZIOMEK ◽  
DANIELA USHIZIMA ◽  
PRZEMYSLAW OBERBEK ◽  
KRZYSZTOF JAN KURZYDŁOWSKI ◽  
TOMASZ PUZYN ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Data ◽  
Scanning Electron Microscopy Image ◽  
Electron Microscopy Image ◽  
Microscopy Image ◽  
Scanning Electron

scholarly journals Use of different SEM techniques in the study of Tyrophagus putrescentiae (Acari: Acaridae) in Costa Rica

2013 ◽  
Vol 5 (2) ◽  
pp. 201-208
Author(s):  
Pamela Murillo ◽  
Hugo Aguilar ◽  
Ethel Sanchez
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Costa Rica ◽  
Morphological Characters ◽  
Morphological Description ◽  
Tyrophagus Putrescentiae ◽  
Viable Option ◽  
Efficient Procedure ◽  
Advantages And Disadvantages ◽  
Scanning Electron

Scanning electron microscopy (SEM) has been used as a tool to facilitate the morphological description of mites and to complement the information obtained from the light microscope. Unfortunately, several of SEM techniques used for soft-bodied mites such as Tyrophagus, have in many cases not shown the desired results. This investigation sought to develop an efficient procedure for preparing specimens of T. putrescentiae for SEM so that its major morphological characters could be observed and photographed. This species is the most important contaminant of tissue culture laboratories in Costa Rica. Seven methods were used to process the samples. The evaluated treatments showed differences in their ability to preserve the morphological structures of the specimens. The advantages and disadvantages of each treatment are discussed.  Mites processed using ethanol were the most viable option with method 3 being the procedure that showed the best results; this technique also reduced the time and costs of processing the specimens.KEY WORDSAcari, Acaridae, Tyrophagus putrescentiae, Scanning Electron Microscopy (SEM), SEM techniques

A new species of Acanthella Schmidt, 1862 (Porifera, Demospongiae, Dictyonellidae) from northeast region, Brazil

Zootaxa ◽  
2019 ◽  
Vol 4555 (2) ◽  
pp. 291
Author(s):  
ELIELTON NASCIMENTO ◽  
THAYNÃ CAVALCANTI ◽  
ULISSES PINHEIRO
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Light Microscopy ◽  
Valid Species ◽  
Taxonomic Identification ◽  
Herbaceous Species ◽  
Northeast Region ◽  
A New Species ◽  
Scanning Electron

Acanthella Schmidt, 1862 species are characterized by choanosomal skeleton of dendritic tracts cored by interwoven sinuously bent strongyles or strongyloxeas and echinated by straight styles, oxeas or anisoxeas (Van Soest et al. 2002). The genus Acanthella comprises 33 valid species, with six known from the Atlantic Ocean (Van Soest et al. 2018). Previously, the only record for Brazil was A. flagelliformis (Van Soest & Stentoft, 1988) (Muricy 2018). Species of the genus include a transition from shrub like ‘Axinellas’ and herbaceous species similar to the genus Suberites Nardo, 1833 (Schmidt 1862; Vosmaer 1912). Due to its heterogeneous complex of species, Acanthella has been confused with other axinellids. Similarities were seen in genera such as Phakellia (Van Soest et al. 2002), which shares spicule complement and comparable choanosomal skeletons. In the present study, we describe a new species of Acanthella from oceanographic expeditions of the northeast region of Brazil. Two specimens of Acanthella sp. nov. were collected by dredging at Ceará and Pernambuco State, Brazil. All specimens were preserved in 92% ethanol and deposited in the Porifera Collections at the Universidade Federal de Pernambuco (UFPEPOR). The taxonomic identification was carried out through analysis of spicules morphologies, using Light Microscopy (LM) and Scanning Electron Microscopy (SEM), following methods described by Hajdu et al. (2011). 

Egg morphology update based on new chorionic data of Potamanthus luteus  (Linnaeus), Ephemera danica Müller and Oligoneuriella rhenana (Imhoff) (Insecta, Ephemeroptera) obtained by scanning electron microscopy

Zootaxa ◽  
2007 ◽  
Vol 1465 (1) ◽  
pp. 15-29 ◽  
Author(s):  
NICOLÁS UBERO-PASCAL ◽  
M. ANGELS PUIG
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Adhesive Layer ◽  
Morphological Data ◽  
Current Trends ◽  
Sem Study ◽  
Egg Morphology ◽  
Ephemera Danica ◽  
Scanning Electron

The chorionic patterns of Ephemeroptera eggs are very diverse and these have often been used for taxonomic and systematic purposes. In a great number of species, including Potamanthus luteus, Ephemera danica and Oligoneuriella rhenana, these egg features have been studied using light microscopy. However, current trends in egg morphology studies use scanning electron microscopy (SEM), so that the eggs of these species need to be re-described in order to establish morphological comparisons. The general chorionic features which have already been described in these three species are confirmed in our SEM study, although a more detailed description of both the architecture and arrangement of these can now be offered. In addition, this study has allowed us to note new morphological data, such as the chorionic reticulation in P. luteus and the complex extrachorion-adhesive layer in E. danica; classification of the lateral attachment structure in P. luteus and O. rhenana has been changed; and the variability of the polar cap observed in P. luteus underlines the care that must to be taken when selecting chorionic structures for taxonomic purposes.

scholarly journals Studies on the genus Setaria Viborg, 1795 in South Africa. III. Setaria thwaitei Mönnig, 1933

2004 ◽  
Vol 71 (2) ◽  
Author(s):  
R. Watermeyer ◽  
J. Boomker ◽  
J.F. Putterill
Keyword(s):  
Electron Microscopy ◽  
South Africa ◽  
Scanning Electron Microscopy ◽  
Original Description ◽  
Valid Species ◽  
The North ◽  
Roan Antelope ◽  
Hippotragus Equinus ◽  
North And South ◽  
Scanning Electron

Mönnig (1933) described Setaria thwaitei from a sable antelope, Hippotragus niger, the type host, as well as from roan antelope, Hippotragus equinus, and waterbuck, Kobus ellipsiprymnus. Yeh (1959) considered Setaria thwaitei to be synonym of Setaria hornbyi. Material collected from roan antelopes, sable antelopes and gemsbuck, Oryx gazella, from several localities in the north and south of South Africa, together with Mönnig's (1933) material, were re-examined. Measurements of the adult worms obtained in this study were compared with those in the original description of the species. Scanning electron microscopy of the anterior and posterior regions of the female worms confirmed S. thwaitei as a valid species.

scholarly journals Anisakidae and Raphidascarididae larvae parasitizing Selene setapinnis (Mitchill, 1815) in the State of Rio de Janeiro, Brazil

2015 ◽  
Vol 24 (1) ◽  
pp. 72-77 ◽  
Author(s):  
Gabrielle Fontenelle ◽  
Marcelo Knoff ◽  
Nilza Nunes Felizardo ◽  
Eduardo José Lopes Torres ◽  
Leila Maria da Silva Lopes ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Rio De Janeiro ◽  
Abdominal Cavity ◽  
Infection Intensity ◽  
Morphological Data ◽  
Mean Intensity ◽  
Nematode Larvae ◽  
Anisakid Nematodes ◽  
Scanning Electron

Between February and August, 2012, thirty specimens of Atlantic moonfish, Selene setapinnis, were purchased in local markets in Niterói, State of Rio de Janeiro, Brazil, with the aim of analyzing the presence of anisakid nematodes, establishing their rates of parasitism and infection sites, due to importance in the sanitary inspection. A total of sixty nematode larvae, belonging to at least two species were found: nine larvae of Terranova sp., Anisakidae, with prevalence (P) of 13.3%, mean intensity (MI) of 2.25, mean abundance (MA) of 0.30 and range of infection intensity (RI) from 1 to 6; and 51 larvae of Hysterothylaciumfortalezae, Raphidascarididae, with P = 26.7%, MI = 6.40, MA = 1.70, and RI = 1-17. The infection sites for Terranova sp. were the mesentery and liver serosa; and for H. fortalezae, the infection sites were the mesentery, abdominal cavity and liver serosa. New morphological data from scanning electron microscopy, on the external structures of H. fortalezae (mainly at the posterior end), are presented. This is the first report of H. fortalezae parasitizing S. setapinnis.

scholarly journals Scanning electron microscopy of acrothoracican cypris larvae (Crustacea, Thecostraca, Cirripedia, Acrothoracica, Lithoglyptidae)

1999 ◽  
Vol 68 (3) ◽  
pp. 143-160 ◽  
Author(s):  
Gregory A. Kolbasov ◽  
Jens T. Høeg ◽  
Alexei S. Elfimov
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Description ◽  
Fourth Segment ◽  
Lattice Organs ◽  
Attachment Organ ◽  
Scanning Electron

Scanning electron microscopy was used to provide a full morphological description of cypris morphologyin the acrothoracican species Lithoglyptes milis and L. habei (Lithoglyptidae).Special attention was givento lattice organs, antennules, thorax, thoracopods, abdomen, and furcal rami. Cypris larvae of the Acrothoracica share some putative plesiomorphic features with the cypris-like ascothoracid larvae of the non-cirripede taxon Ascothoracida. The most notable are traces of abdominal segmentation and carapace lattice organs without pore fields. Acrothoracican cyprids also share numerous synapomorphies with those of the Thoracica and the Rhizocephala. This list includes a four-segmented antennule with a triangular first segment of two sclerites set at an angle to each other, a cylindrical second segment, a small third segment functioning asan attachment organ, and a cylindrical fourth segment bearinghomologous sensory setae. Further apomorphies are a pair of frontolateral horn glands exiting anteroventrally on the headshield (carapace), a pair of multicellular cement glands exiting on the attachment organs, a single stout, serrated and non-natatory seta on the thoracopodal exopods and a highly reduced abdomen with at best traces of segmentation. These synapomorphies in cypris morphology supporta monophyletic taxon Cirripedia comprising the Acrothoracica, Thoracica, and Rhizocephala but excluding the Ascothoracida.

Early morphological development of gills in smallmouth bass (Micropterus dolomieui)

1984 ◽  
Vol 62 (5) ◽  
pp. 951-958 ◽  
Author(s):  
David J. Coughlan ◽  
Steven P. Gloss
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Smallmouth Bass ◽  
Gill Arch ◽  
Morphological Description ◽  
Morphological Development ◽  
Secondary Lamellae ◽  
Micropterus Dolomieui ◽  
First 90 Days ◽  
Scanning Electron

The morphological development of the gill apparatus in 11- to 180-day old smallmouth bass (Micropterus dolomieui) is described as seen by scanning electron microscopy. Filament protrusions without lamellae developed on the midregion of the gill arches in yolk-sac fry. Filament development progressed from the midregion of the gill arch toward the insertions. Secondary lamellae were first observed 15 days after egg fertilization when the fry became free swimming. Lamellae first appeared on filaments proximal to the gill arch and advanced distally. Proliferation of filaments and lamellae continued through at least the first 90 days of life. Filaments on the medial hemibranch of a holobranch were longer than those on the lateral hemibranch for at least the first 90 days. Filaments were alternately arranged between two hemibranchs of a holobranch. Substantial differences existed between gill developmental stage at hatching in smallmouth bass and that previously reported for salmonids. The dynamic nature of the developing gill indicated that quantitative descriptions of filament length, lamellar number and area, and total gill surface area requires examination of specimens at numerous different ages. This is the first morphological description of developing gills in a teleost fish based on observations by scanning electron microscopy.

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