Redescription of Diplogasteroides nasuensis Takaki, 1941 and D. magnus Völk, 1950 (Nematoda: Diplogastrina) associated with Scarabaeidae (Coleoptera)

Nematology ◽  
2001 ◽  
Vol 3 (8) ◽  
pp. 817-832 ◽  
Author(s):  
Walter Sudhaus ◽  
Karin Kiontke ◽  
Albrecht Manegold
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Broad Sense ◽  
Morphological Description ◽  
Scanning Electron

AbstractA detailed morphological description is presented of Diplogasteroides nasuensis Takaki, 1941 and D. magnus Völk, 1950, using light and scanning electron microscopy. Neoaplectana melolonthae Weiser, 1958 and Diplogasteroides (Rhabdontolaimus) berwigi Rühm, 1959 are synonymised with D. nasuensis. This gonochoristic species is distinguished from the hermaphroditic D. magnus by the dorsal metastomal decoration which consists of bristle-like protrusions instead of a more or less uniform tooth. The spermatocytes in D. nasuensis are twice as big as those in hermaphrodites of D. magnus. Dauer juveniles of D. nasuensis are considerably bigger than those of D. magnus. Dauer juveniles of both species are present on wood cockchafer (Melolontha hippocastani) larvae and adults but, on adult beetles, D. nasuensis dauer juveniles enter the genital pouch, whereas those of D. magnus are found under the hind coxae. In Germany, D. nasuensis is found only on M. hippocastani. D. magnus dauer juveniles are also found on a variety of other Scarabaeidae. Further small differences distinguish the two species. The genus Diplogasteroides is proposed to be taken in a broad sense. Several genera names are synonymised with Diplogasteroides.

scholarly journals Use of different SEM techniques in the study of Tyrophagus putrescentiae (Acari: Acaridae) in Costa Rica

2013 ◽  
Vol 5 (2) ◽  
pp. 201-208
Author(s):  
Pamela Murillo ◽  
Hugo Aguilar ◽  
Ethel Sanchez
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Costa Rica ◽  
Morphological Characters ◽  
Morphological Description ◽  
Tyrophagus Putrescentiae ◽  
Viable Option ◽  
Efficient Procedure ◽  
Advantages And Disadvantages ◽  
Scanning Electron

Scanning electron microscopy (SEM) has been used as a tool to facilitate the morphological description of mites and to complement the information obtained from the light microscope. Unfortunately, several of SEM techniques used for soft-bodied mites such as Tyrophagus, have in many cases not shown the desired results. This investigation sought to develop an efficient procedure for preparing specimens of T. putrescentiae for SEM so that its major morphological characters could be observed and photographed. This species is the most important contaminant of tissue culture laboratories in Costa Rica. Seven methods were used to process the samples. The evaluated treatments showed differences in their ability to preserve the morphological structures of the specimens. The advantages and disadvantages of each treatment are discussed.  Mites processed using ethanol were the most viable option with method 3 being the procedure that showed the best results; this technique also reduced the time and costs of processing the specimens.KEY WORDSAcari, Acaridae, Tyrophagus putrescentiae, Scanning Electron Microscopy (SEM), SEM techniques

scholarly journals Detailed morphological description of Habronema clarki Foster & Chitwood, 1937, a nematode parasite of capybaras Hydrochoerus hydrochaeris (Linnaeus, 1766) in Brazil

2014 ◽  
Vol 23 (2) ◽  
pp. 251-254
Author(s):  
Estevam Guilherme Lux Hoppe ◽  
José Hairton Tebaldi ◽  
Daniel Fontana Ferreira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nematode Parasite ◽  
Morphological Data ◽  
Valid Species ◽  
Morphological Description ◽  
Hydrochoerus Hydrochaeris ◽  
Scanning Electron

The genus Habronema has four valid species, of which only two are properly known. The present study aimed to describe in detail the morphology of Habronema clarki through optical and scanning electron microscopy analyses. Our results showed that the labial morphology of this parasite is closer to H. muscae than to H. microstoma. Even so, the characteristic pseudolabia and the slightly convex border of the dorsal and ventral lips are sufficient to distinguish these nematodes. Additional morphological data are presented, thus contributing to the knowledge on this little known nematode. In addition, this study provides new locality records for this species.

scholarly journals Scanning electron microscopy of acrothoracican cypris larvae (Crustacea, Thecostraca, Cirripedia, Acrothoracica, Lithoglyptidae)

1999 ◽  
Vol 68 (3) ◽  
pp. 143-160 ◽  
Author(s):  
Gregory A. Kolbasov ◽  
Jens T. Høeg ◽  
Alexei S. Elfimov
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Description ◽  
Fourth Segment ◽  
Lattice Organs ◽  
Attachment Organ ◽  
Scanning Electron

Scanning electron microscopy was used to provide a full morphological description of cypris morphologyin the acrothoracican species Lithoglyptes milis and L. habei (Lithoglyptidae).Special attention was givento lattice organs, antennules, thorax, thoracopods, abdomen, and furcal rami. Cypris larvae of the Acrothoracica share some putative plesiomorphic features with the cypris-like ascothoracid larvae of the non-cirripede taxon Ascothoracida. The most notable are traces of abdominal segmentation and carapace lattice organs without pore fields. Acrothoracican cyprids also share numerous synapomorphies with those of the Thoracica and the Rhizocephala. This list includes a four-segmented antennule with a triangular first segment of two sclerites set at an angle to each other, a cylindrical second segment, a small third segment functioning asan attachment organ, and a cylindrical fourth segment bearinghomologous sensory setae. Further apomorphies are a pair of frontolateral horn glands exiting anteroventrally on the headshield (carapace), a pair of multicellular cement glands exiting on the attachment organs, a single stout, serrated and non-natatory seta on the thoracopodal exopods and a highly reduced abdomen with at best traces of segmentation. These synapomorphies in cypris morphology supporta monophyletic taxon Cirripedia comprising the Acrothoracica, Thoracica, and Rhizocephala but excluding the Ascothoracida.

Early morphological development of gills in smallmouth bass (Micropterus dolomieui)

1984 ◽  
Vol 62 (5) ◽  
pp. 951-958 ◽  
Author(s):  
David J. Coughlan ◽  
Steven P. Gloss
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Smallmouth Bass ◽  
Gill Arch ◽  
Morphological Description ◽  
Morphological Development ◽  
Secondary Lamellae ◽  
Micropterus Dolomieui ◽  
First 90 Days ◽  
Scanning Electron

The morphological development of the gill apparatus in 11- to 180-day old smallmouth bass (Micropterus dolomieui) is described as seen by scanning electron microscopy. Filament protrusions without lamellae developed on the midregion of the gill arches in yolk-sac fry. Filament development progressed from the midregion of the gill arch toward the insertions. Secondary lamellae were first observed 15 days after egg fertilization when the fry became free swimming. Lamellae first appeared on filaments proximal to the gill arch and advanced distally. Proliferation of filaments and lamellae continued through at least the first 90 days of life. Filaments on the medial hemibranch of a holobranch were longer than those on the lateral hemibranch for at least the first 90 days. Filaments were alternately arranged between two hemibranchs of a holobranch. Substantial differences existed between gill developmental stage at hatching in smallmouth bass and that previously reported for salmonids. The dynamic nature of the developing gill indicated that quantitative descriptions of filament length, lamellar number and area, and total gill surface area requires examination of specimens at numerous different ages. This is the first morphological description of developing gills in a teleost fish based on observations by scanning electron microscopy.

Larval development of the barnacle Ibla cumingi (Cirripedia: Pendunculata: Iblidae) reared in the laboratory

2005 ◽  
Vol 85 (4) ◽  
pp. 903-907 ◽  
Author(s):  
Yan Yan ◽  
Chen Haoru ◽  
Huang Liangmin ◽  
Sun Lihua
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Larval Development ◽  
Development Model ◽  
Daya Bay ◽  
Morphological Description ◽  
The Difference ◽  
Cephalic Shield ◽  
Scanning Electron

Larvae of Ibla cumingi from Daya Bay, China were cultured in the laboratory. Larval development includes six naupliar stages and a non-feeding cypris stage following the ground patterns of cirripeds. Larvae reached the cypris stage in nine days at ∼25°C after hatching. Morphological features including the cephalic shield, frontal horns, labrum, abdominal process, antennules, antennae and mandibles in all nauplii were described and illustrated using light microscopy. A full morphological description of cyprid larvae was provided using scanning electron microscopy. Attempts were made to compare the difference between the larval development model of I. cumingi and that of Ibla species.

scholarly journals Primeros registros e intervalos de distribución de Holothuria (Theelothuria) princeps Selenka, 1867 (Echinodermata: Holothuroidea: Holothuriidae) en el golfo de Morrosquillo, Caribe Colombiano

2019 ◽  
Vol 54 (1) ◽  
pp. 139
Author(s):  
Carlos Carlos Nisperuza-Pérez ◽  
Jorge Quirós-Rodríguez ◽  
Francisco Alonso Solís-Marín
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Spatial Distribution ◽  
Geographic Distribution ◽  
Morphological Description ◽  
Caribbean Coast ◽  
The Caribbean ◽  
Microscopy Images ◽  
Scanning Electron

The geographic distribution of Holothuria (Theelothuria) princeps in Colombian Caribbean is extended inside the Gulf of Morrosquillo, based on 7 individuals collected in Banco de Arena sector, Córdoba. A morphological description of this species record with color figures and scanning electron microscopy images of their ossicles is provided. This finding increases the spatial distribution of this species in the Caribbean coast of Colombia.

scholarly journals Morphological approach focusing on the structure of the external genitalia of female Triatoma jatai, Triatoma costalimai, and Triatoma williami (Hemiptera: Reduviidae: Triatominae)

2020 ◽  
Author(s):  
Simone Caldas Teves ◽  
Teresa Cristina Monte Gonçalves ◽  
Simone Patricia Carneiro de Freitas ◽  
Catarina Macedo Lopes ◽  
Ana Laura Carbajal-de-la-Fuente ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Natural Infection ◽  
External Genitalia ◽  
Biological Species ◽  
Morphological Description ◽  
Adhesive Tape ◽  
Reference Laboratory ◽  
Dichotomous Key ◽  
Scanning Electron

Abstract BackgroudTaxonomic identification of triatomines is generally performed based on aspects of their external morphology. However, the use of a multidisciplinary approach, considering morphological aspects of the external genitalia, morphometry, genetics, and phylogeography has been suggested, especially for similar and/or cryptic species. The rupestral species Triatoma jatai Gonçalves et al. 2013, Triatoma costalimai Verano & Galvão 1959, and Triatoma williami Galvão et al. 1965, which are morphologically similar, have been found with natural infection by Trypanosoma cruzi (Chagas, 1930) in wild, peridomestic, and intradomestic environments, representing a risk of new outbreaks of Chagas disease. This study presents a morphological description complementation of these species, with an emphasis on the structures of the female external genitalia, using scanning electron microscopy. MethodsFemale specimens of T. jatai and T. costalimai captured in Brazilian Municipalites of Paranã and Aurora do Tocantins were identified with the use of a dichotomous key for the sub-complex Matogrossensis, as well T. williami, obtained by colony of reference laboratory in triatomines. The insects were sectioned with a scalpel in the transverse direction at the height of the sixth abdominal segment. The terminal portion of the abdomen was washed, dehydrated in an alcoholic series and mounted on a metallic support on double-sided adhesive tape in the ventral or dorsal positions. Samples were then left in an oven and in a desiccator containing silica gel for drying until metallization. They were metallized with gold and examined using a scanning electron microscope at the Oswaldo Cruz/FIOCRUZ Institute Electronic Microscopy Platform. ResultsIt was possible to differentiate between the species based on characteristics of the 7th, 8th, and 9th urotergites and 7th urosternite, as well as the genital plaques, gonocoxites, and gonapophyses. For the first time, morphological differences in the thorns present on gonocoxite 8 are described in triatomines.ConclusionsWe results show that external genitalia of female it is a useful structure to differentiate T. jatai, T. costalimai and T. williami. The scanning electron microscopy contribute and corroborate, together with other tools morphological and molecular, to the differentiation of T. jatai, T. costalimai and T. williami, as well as expand the taxonomic knowledge. It may also help to understand if there is reproductive compatibility between T. jatai and T. costalimai as well possible formation of hybrids under artificial conditions. This one is underway in order to fully contemplate all definitions of biological species.

Oricymba gongshanensis sp. nov. (Cymbellaceae; Bacillariophyceae), a new species from southwest China

Phytotaxa ◽  
2021 ◽  
Vol 508 (1) ◽  
Author(s):  
JI-SHU GUO ◽  
WEN-WEN MENG ◽  
XIAO-YANG HE ◽  
YUN ZHANG ◽  
YAN-LING LI ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Yunnan Province ◽  
Southwest China ◽  
Morphological Description ◽  
Single Opening ◽  
A New Species ◽  
Scanning Electron ◽  
Apical Axis

Oricymba gongshanensis sp. nov. is identified as a new cymbelloid species, and described from a rock wall in Gongshan County, Yunnan Province, China. A detailed morphological description of the new species is presented, based on light and scanning electron microscopy. O. gongshanensis sp. nov. has the features that assign it to the genus Oricymba, including a thickened ridge positioned around the margin of the valve, presence of pore fields at the apices, a stigma with a single opening externally and two openings internally together with external distal raphe ends deflected dorsally. There are three features that easily distinguish the new species from all others in the genus. They include: 1) valves that are weakly asymmetrical about the apical axis, 2) presence of mostly biseriate striae and 3) areolae that are round to elliptical in shape. Among the currently described species of Oricymba, the new species is most similar to O. tianmuensis in valve shape, but is easily distinguished by the degree of asymmetry about the apical axis and presence of biseriate striae. These findings increase our understanding the morphology and distribution of the genus Oricymba in China.

Cymbella xiaojinensis sp. nov., a new cymbelloid diatom species (Bacillariophyceae) from high altitude lakes, China

Phytotaxa ◽  
2021 ◽  
Vol 482 (1) ◽  
pp. 55-64
Author(s):  
YUN ZHANG ◽  
MENGNA LIAO ◽  
YAN-LING LI ◽  
FENG-QIN CHANG ◽  
JOHN PATRICK KOCIOLEK
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Central Area ◽  
Morphological Characteristics ◽  
Morphological Description ◽  
Hengduan Mountains ◽  
Diatom Species ◽  
A New Species ◽  
Scanning Electron

This paper describes a new species of Cymbella from an alpine lake in the Hengduan Mountains, southwestern China. The detailed morphological description of the new species, named Cymbella xiaojinensis sp. nov. is presented, based on light and scanning electron microscopy. The main features of C. xiaojinensis are characterized by strongly dorsiventral, triangular valves with a strongly reverse-lateral raphe system positioned near the proximal ends and an asymmetrical, larger central area, occupying approximately 1/2 of the width with the valve. Most morphological characteristics of the new species are similar to C. shudunensis, C. terrafuegiana, C. pamirensis, and C. gravida, but differ in details of size, valve shape, striae density, central area, and number of stigmata.

Cymbella hechiensis sp. nov., a new cymbelloid diatom species (Bacillariophyceae) from the upper tributary of Liujiang River, Guangxi Province, China

Phytotaxa ◽  
2019 ◽  
Vol 425 (1) ◽  
pp. 49-56
Author(s):  
YAN-LING LI ◽  
MING-YAN BAO ◽  
WEI LIU ◽  
JOHN PATRICK KOCIOLEK ◽  
WEI ZHANG
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Ventral Side ◽  
Morphological Description ◽  
Diatom Species ◽  
Guangxi Province ◽  
Freshwater Diatoms ◽  
Scanning Electron

During a survey of freshwater diatoms from streams in the upper reaches of the Liujiang River, Guangxi Province, China, a new Cymbella species, Cymbella liujiangensis sp. nov. was recorded. A detailed morphological description of C. liujiangensis is presented, based on light and scanning electron microscopy. The main features of C. liujiangensis include strongly dorsiventral valves with slightly truncate and obtusely rounded ends and one very large, transaspically-elongated stigma positioned on the ventral side of the central nodule and adjacent to the middle ventral striae. The new species is compared with C. dorsenotata, C. aspera, C. peraspera, C. australica, C. praerupta, C. convexa, C. crassistigmata, C. graciliformis and C. simonsenii, all of which show similarities to Cymbella liujiangensis, but differ in details of size, valve shape, striae density, puncta density, and number of stigmata.

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