scholarly journals Transition from Preinvasive Carcinoma In Situ to Seminoma Is Accompanied by a Reduction of Connexin 43 Expression in Sertoli Cells and Germ Cells

Neoplasia ◽  
2006 ◽  
Vol 8 (6) ◽  
pp. 499-509 ◽  
Author(s):  
Ralph Brehm ◽  
Christina Ruttinger ◽  
Petra Fischer ◽  
Isabella Gashaw ◽  
Elke Winterhager ◽  
...  
Keyword(s):  
Germ Cells ◽  
Sertoli Cells ◽  
Carcinoma In Situ ◽  
Connexin 43

scholarly journals Expression Analysis ofGnrh1andGnrhr1in Spermatogenic Cells of Rat

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Vincenza Ciaramella ◽  
Rosanna Chianese ◽  
Paolo Pariante ◽  
Silvia Fasano ◽  
Riccardo Pierantoni ◽  
...  
Keyword(s):  
Germ Cells ◽  
Sertoli Cells ◽  
Leydig Cells ◽  
Spermatogenic Cells ◽  
Myoid Cells ◽  
Quantitative Real Time Pcr ◽  
The Past ◽  
Expression Rate ◽  
Sperm Functions

Hypothalamic Gonadotropin Releasing Hormone (GnRH),viaGnRH receptor (GnRHR), is the main actor in the control of reproduction, in that it induces the biosynthesis and the release of pituitary gonadotropins, which in turn promote steroidogenesis and gametogenesis in both sexes. Extrabrain functions of GnRH have been extensively described in the past decades and, in males, local GnRH activity promotes the progression of spermatogenesis and sperm functions at several levels. The canonical localization ofGnrh1andGnrhr1mRNA is Sertoli and Leydig cells, respectively, but ligand and receptor are also expressed in germ cells. Here, we analysed the expression rate ofGnrh1andGnrhr1in rat testis (180 days old) by quantitative real-time PCR (qPCR) and byin situhybridization we localizedGnrh1andGnrhr1mRNA in different spermatogenic cells of adult animals. Our data confirm the testicular expression ofGnrh1and ofGnrhr1in somatic cells and provide evidence that their expression in the germinal compartment is restricted to haploid cells. In addition, not only Sertoli cells connected to spermatids in the last steps of maturation but also Leydig and peritubular myoid cells expressGnrh1.

Altered expression of connexins 26 and 43 in Sertoli cells in seminiferous tubules infiltrated with carcinoma-in-situ or seminoma

2002 ◽  
Vol 197 (5) ◽  
pp. 647-653 ◽  
Author(s):  
Ralph Brehm ◽  
Alexander Marks ◽  
Rodolfo Rey ◽  
Sabine Kliesch ◽  
Martin Bergmann ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Carcinoma In Situ ◽  
Seminiferous Tubules ◽  
Altered Expression

scholarly journals Carcinoma in situ testis displays permissive chromatin modifications similar to immature foetal germ cells

2010 ◽  
Vol 103 (8) ◽  
pp. 1269-1276 ◽  
Author(s):  
K Almstrup ◽  
J E Nielsen ◽  
O Mlynarska ◽  
M T Jansen ◽  
A Jørgensen ◽  
...  
Keyword(s):  
Germ Cells ◽  
Carcinoma In Situ ◽  
Chromatin Modifications

Investigation of the potential role of the germ cell complement in control of the expression of transferrin mRNA in the prepubertal and adult rat testis

1997 ◽  
Vol 19 (1) ◽  
pp. 67-77 ◽  
Author(s):  
S M Maguire ◽  
M R Millar ◽  
R M Sharpe ◽  
J Gaughan ◽  
P T K Saunders
Keyword(s):  
Germ Cell ◽  
Mrna Expression ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Direct Access ◽  
Adult Rats ◽  
Rat Testis ◽  
Adult Rat

ABSTRACT Iron is required for the normal development of germ cells during spermatogenesis. Because these cells have no direct access to systemic iron, there exists a shuttle system involving production and secretion of the iron-transporting protein transferrin by the Sertoli cells. Previous reports using cultures of immature Sertoli cells exposed to adult germ cells, or in vivo studies involving germ cell-depleted adult rat testes, concluded that production of transferrin by Sertoli cells is modulated by germ cell complement. In the present study we have used in situ hybridisation with cRNA probes directed against the 5′ and 3′ ends of transferrin mRNA to examine the pattern of expression of transferrin in the immature and adult rat testis. Adult rats were treated with ethane dimethane sulphonate or methoxyacetic acid (MAA) to manipulate their testosterone levels or germ cell complement respectively. Initial findings obtained using the 3′ probe showed a decrease in transferrin mRNA associated with round spermatid depletion. However, these data were not confirmed by in situ hybridisation when the 5′ probe was used. The specificity of the probes was examined using Northern blotting and the 3′ probe was found to hybridise to the germ cell transcript for hemiferrin even under conditions of high stringency. Examination of immature and pubertal rat testes by in situ hybridisation using the 5′ transferrin-specific probe found that as early as 14 days of age the level of expression of transferrin mRNA was clearly different between tubules, and the mRNA appeared to be expressed in Leydig cells on and after day 31. In the adult rat testis, maximal expression of transferrin mRNA was found at stages VIII-XIV, calling into question the interpretation of the results of some previous studies showing expression of transferrin mRNA at all stages of the spermatogenic cycle. This stage-specific pattern of expression was not altered by acute germ cell depletion using MAA. However, Northern blot analysis showed a statistically significant increase in transferrin mRNA expression at 7 days after MAA treatment when pachytene spermatocytes were depleted from tubules at all stages of the spermatogenic cycle at which transferrin is normally expressed. In conclusion, we found that transferrin mRNA expression was not modulated by round spermatids as has been reported previously but that meiotic germ cells may influence expression of transferrin at specific stages of the spermatogenic cycle.

Immunohistochemical markers of carcinoma in situ of the testis also expressed in normal infantile germ cells

1993 ◽  
Vol 22 (4) ◽  
pp. 373-378 ◽  
Author(s):  
N. JφRGENSEN ◽  
A. GIWERCMAN ◽  
J. MÜLLER ◽  
N. E. SKAKKEBÆK
Keyword(s):  
Germ Cells ◽  
Carcinoma In Situ ◽  
Immunohistochemical Markers

scholarly journals Vitamin K-dependent γ-glutamyl carboxylase in Sertoli cells is essential for male fertility in mice

2021 ◽  
Author(s):  
Sachiko Shiba ◽  
Ikeda Kazuhiro ◽  
Kuniko Horie-Inoue ◽  
Kotaro Azuma ◽  
Tomoka Hasegawa ◽  
...  
Keyword(s):  
Vitamin K ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Connexin 43 ◽  
Late Onset ◽  
Sperm Concentration ◽  
Conditional Knockout ◽  
Seminiferous Tubules ◽  
Junction Protein ◽  
Gap Junction Protein

Gamma-glutamyl carboxylase (GGCX) is a vitamin K (VK)-dependent enzyme that catalyzes γ-carboxylation of glutamic acid residues in VK-dependent proteins. Anticoagulant warfarin is known to reduce GGCX activity by inhibiting VK cycle and is recently shown to disrupt spermatogenesis. To explore GGCX function in testis, we here generated Sertoli cell-specific Ggcx conditional knockout (Ggcx scKO) mice and investigated their testicular phenotype. Ggcx scKO mice exhibited late-onset male infertility. They possessed morphologically abnormal seminiferous tubules containing multinucleated and apoptotic germ cells, and their sperm concentration and motility were substantially reduced. Localization of connexin 43 (Cx43), a gap junction protein abundantly expressed in Sertoli cells and required for spermatogenesis, was distorted in Ggcx scKO testes, and Cx43 overexpression in Sertoli cells rescued the infertility of Ggcx scKO mice. These results highlight GGCX activity within Sertoli cells, which promotes spermatogenesis by regulating the intercellular connection between Sertoli cells and germ cells.

scholarly journals Altered Expression of ZO-1 and ZO-2 in Sertoli Cells and Loss of Blood-Testis Barrier Integrity in Testicular Carcinoma In Situ

Neoplasia ◽  
2006 ◽  
Vol 8 (12) ◽  
pp. 1019-1027 ◽  
Author(s):  
Cornelia Fink ◽  
Roswitha Weigel ◽  
Tanja Hembes ◽  
Heidrun Lauke-Wettwer ◽  
Sabine Kliesch ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Carcinoma In Situ ◽  
Altered Expression ◽  
Barrier Integrity ◽  
Testicular Carcinoma ◽  
Blood Testis Barrier
Sign in / Sign up

Export Citation Format

Share Document