Exemplar Abstract for Neisseria flavescens Branham 1930 (Approved Lists 1980).

ABSTRACT Neisseria gonorrhoeae strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 μg/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, N. gonorrhoeae ATCC 19424, by transformation of the penicillin-binding protein 2 gene (penA) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 μg/ml). The sequences of penA in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported N. gonorrhoeae penA gene sequences. Some regions in the transpeptidase-encoding domain in this penA gene were similar to those in the penA genes of Neisseria perflava (N. sicca), Neisseria cinerea, Neisseria flavescens, and Neisseria meningitidis. These results showed that a mosaic-like structure in the penA gene conferred reductions in the levels of susceptibility of N. gonorrhoeae to cephems and penicillin in a manner similar to that found for N. meningitidis and Streptococcus pneumoniae.

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Septicaemia caused by Neisseria flavescens

Journal of Clinical Pathology ◽

10.1136/jcp.21.4.437 ◽

1968 ◽

Vol 21 (4) ◽

pp. 437-439 ◽

Cited By ~ 14

Author(s):

P. T. Wertlake ◽

T. W. Williams

Keyword(s):

Neisseria Flavescens

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Functional diversity of three different DsbA proteins from Neisseria meningitidis

Microbiology ◽

10.1099/mic.0.27216-0 ◽

2004 ◽

Vol 150 (9) ◽

pp. 2993-3000 ◽

Cited By ~ 32

Author(s):

Sunita Sinha ◽

Paul R. Langford ◽

J. Simon Kroll

Keyword(s):

Escherichia Coli ◽

Alkaline Phosphatase ◽

Protein Folding ◽

Membrane Proteins ◽

Neisseria Meningitidis ◽

Outer Membrane Proteins ◽

Invasive Disease ◽

Neisseria Lactamica ◽

Neisseria Subflava ◽

Neisseria Flavescens

The genome of Neisseria meningitidis serogroup B strain MC58 contains three genes – nmb0278, nmb0294 and nmb0407 – encoding putative homologues of DsbA, a periplasmic thiol disulphide oxidoreductase protein-folding catalyst of the Dsb protein family. DsbA assists the folding of periplasmic and membrane proteins in diverse organisms. While all three cloned genes complemented the DTT sensitivity of dsbA-null Escherichia coli, they showed different activities in folding specific target proteins in this background. NMB0278 protein was the most active in complementing defects in motility and alkaline phosphatase activity, while NMB0294 was the most active in folding periplasmic MalF. NMB0407 showed the weakest activity in all assays. It is extremely unusual for organisms to contain more than one chromosomal dsbA. Among the members of the genus Neisseria, only the meningococcus carries all three of these genes. Strains of Neisseria gonorrhoeae, Neisseria lactamica, Neisseria cinerea and Neisseria polysaccharea contained only homologues of nmb0278 and nmb0407, while Neisseria flava, Neisseria subflava and Neisseria flavescens carried only nmb0294. It is speculated that the versatility of the meningococcus in surviving in different colonizing and invasive disease settings may be derived in part from an enhanced potential to deploy outer-membrane proteins, a consequence of carrying an extended repertoire of protein-folding catalysts.

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DNA base composition of Neisseria, Moraxella, and Acinetobacter, as determined by measurement of buoyant density in CsCl gradients

Canadian Journal of Microbiology ◽

10.1139/m69-062 ◽

1969 ◽

Vol 15 (4) ◽

pp. 335-338 ◽

Cited By ~ 20

Author(s):

K. Bøvre ◽

M. Fiandt ◽

W. Szybalski

Keyword(s):

Buoyant Density ◽

Mutual Transformation ◽

Supporting Evidence ◽

Dna Base Composition ◽

Group I ◽

Dna Base ◽

Wide Range ◽

Additional Support ◽

Neisseria Flavescens

The guanine + cytosine contents (%(G + C)) of DNAs from 75 strains of asaccharolytic Neisseria, Moraxella, and Acinetobacter have been determined by measuring their buoyant densities in the CsCl gradient. The main purpose was to provide supporting evidence for taxonomic conclusions based on assay of genetic transformation to streptomycin resistance among the same strains and species.Three groups of neisseriae can be recognized, both by determination of %(G + C) and by transformation assay: (i) Neisseria flavescens and N. cinerea (46.5–49%), (ii) N. catarrhalis (41–42.5%), and (iii) N. caviae and N. ovis (44.5–45%). There is no transformation compatibility between group (i) and the other groups, whereas groups (ii) and (iii) show mutual transformation interactions. N. catarrhalis, N. caviae, and N. ovis, therefore, can also be considered as one group of nonpigmented, asaccharolytic neisseriae.Four groups of moraxellae can be distinguished: (i) Moraxella nonliquefaciens, M. lacunata, M. liquefaciens, and M. bovis (40–43%), (ii) M. osloensis (43–43.5%), (iii) M. phenylpyrouvica (43–43.5%), and (iv) M. kingii (44.5%). Although groups (ii) and (iii) are identical in terms of %(G + C), they exhibit low transformation compatibility, of the same order as between groups (i) and (ii). The distinctly higher G + C content of M. kingii DNA is consistent with its lack of transformation compatibility with other moraxellae.The similar %(G + C) range for the moraxellae and the nonpigmented, asaccharolytic neisseriae is consistent with the finding of some transformation compatibility between most of these organisms (except M. kingii) and provides additional support for the assumption that they are closely related.The Acinetobacter strains studied exhibit a wide range of G + C contents (38–45%) and hardly any transformation compatibility with neisseriae and moraxellae, even if some of them have matching %(G + C).It can be concluded that determination of the G + C contents of bacterial DNAs provides useful supplementary taxonomic data, but has only limited value as a sole taxonomic criterion.

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