Exemplar Abstract for Desulfococcus multivorans Widdel 1981.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Desulfococcus Multivorans

scholarly journals Selenocysteine-Containing Proteins in Anaerobic Benzoate Metabolism of Desulfococcus multivorans

2004 ◽  
Vol 186 (7) ◽  
pp. 2156-2163 ◽  
Author(s):  
Franziska Peters ◽  
Michael Rother ◽  
Matthias Boll
Keyword(s):  
Trace Elements ◽  
Specific Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Model Organism ◽  
Strictly Anaerobic ◽  
Coa Ligase ◽  
Coa Reductase ◽  
Aliphatic Carboxylic Acids ◽  
Desulfococcus Multivorans

ABSTRACT The sulfate-reducing bacterium Desulfococcus multivorans uses various aromatic compounds as sources of cell carbon and energy. In this work, we studied the initial steps in the aromatic metabolism of this strictly anaerobic model organism. An ATP-dependent benzoate coenzyme A (CoA) ligase (AMP plus PPi forming) composed of a single 59-kDa subunit was purified from extracts of cells grown on benzoate. Specific activity was highest with benzoate and some benzoate derivatives, whereas aliphatic carboxylic acids were virtually unconverted. The N-terminal amino acid sequence showed high similarities with benzoate CoA ligases from Thauera aromatica and Azoarcus evansii. When cultivated on benzoate, cells strictly required selenium and molybdenum, whereas growth on nonaromatic compounds, such as cyclohexanecarboxylate or lactate, did not depend on the presence of the two trace elements. The growth rate on benzoate was half maximal with 1 nM selenite present in the growth medium. In molybdenum- and/or selenium-depleted cultures, growth on benzoate could be induced by addition of the missing trace elements. In extracts of cells grown on benzoate in the presence of [75Se]selenite, three radioactively labeled proteins with molecular masses of ∼100, 30, and 27 kDa were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. The 100- and 30-kDa selenoproteins were 5- to 10-fold induced in cells grown on benzoate compared to cells grown on lactate. These results suggest that the dearomatization process in D. multivorans is not catalyzed by the ATP-dependent Fe-S enzyme benzoyl-CoA reductase as in facultative anaerobes but rather involves unknown molybdenum- and selenocysteine-containing proteins.

Nomenclature Abstract for Desulfococcus multivorans Widdel 1981.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity
Keyword(s):  
Desulfococcus Multivorans

Extracellular metal-binding activity of the sulphate-reducing bacterium Desulfococcus multivorans

Microbiology ◽  
1999 ◽  
Vol 145 (10) ◽  
pp. 2987-2995 ◽  
Author(s):  
Toni A. M. Bridge ◽  
Geoffrey M. Gadd ◽  
Chris White
Keyword(s):  
Metal Binding ◽  
Binding Activity ◽  
Desulfococcus Multivorans

scholarly journals Polyhydroxyalkanoate (PHA) Accumulation in Sulfate-Reducing Bacteria and Identification of a Class III PHA Synthase (PhaEC) in Desulfococcus multivorans

2004 ◽  
Vol 70 (8) ◽  
pp. 4440-4448 ◽  
Author(s):  
Tran Hai ◽  
Daniela Lange ◽  
Ralf Rabus ◽  
Alexander Steinbüchel
Keyword(s):  
Amino Acid ◽  
Dry Matter ◽  
Sulfate Reducing Bacteria ◽  
Pha Synthase ◽  
Desulfovibrio Vulgaris ◽  
Class Iii ◽  
Sulfate Reducing ◽  
Desulfobacterium Autotrophicum ◽  
Reducing Bacteria ◽  
Desulfococcus Multivorans

ABSTRACT Seven strains of sulfate-reducing bacteria (SRB) were tested for the accumulation of polyhydroxyalkanoates (PHAs). During growth with benzoate Desulfonema magnum accumulated large amounts of poly(3-hydroxybutyrate) [poly(3HB)]. Desulfosarcina variabilis (during growth with benzoate), Desulfobotulus sapovorans (during growth with caproate), and Desulfobacterium autotrophicum (during growth with caproate) accumulated poly(3HB) that accounted for 20 to 43% of cell dry matter. Desulfobotulus sapovorans and Desulfobacterium autotrophicum also synthesized copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyvalerate when valerate was used as the growth substrate. Desulfovibrio vulgaris and Desulfotalea psychrophila were the only SRB tested in which PHAs were not detected. When total DNA isolated from Desulfococcus multivorans and specific primers deduced from highly conserved regions of known PHA synthases (PhaC) were used, a PCR product homologous to the central region of class III PHA synthases was obtained. The complete pha locus of Desulfococcus multivorans was subsequently obtained by inverse PCR, and it contained adjacent phaEDm and phaCDm genes. PhaC Dm and PhaE Dm were composed of 371 and 306 amino acid residues and showed up to 49 or 23% amino acid identity to the corresponding subunits of other class III PHA synthases. Constructs of phaCDm alone (pBBRMCS-2::phaCDm ) and of phaEDmCDm (pBBRMCS-2::phaEDmCDm ) in various vectors were obtained and transferred to several strains of Escherichia coli, as well as to the PHA-negative mutants PHB−4 and GPp104 of Ralstonia eutropha and Pseudomonas putida, respectively. In cells of the recombinant strains harboring phaEDmCDm small but significant amounts (up to 1.7% of cell dry matter) of poly(3HB) and of PHA synthase activity (up to 1.5 U/mg protein) were detected. This indicated that the cloned genes encode functionally active proteins. Hybrid synthases consisting of PhaC Dm and PhaE of Thiococcus pfennigii or Synechocystis sp. strain PCC 6308 were also constructed and were shown to be functionally active.

scholarly journals Genome and catabolic subproteomes of the marine, nutritionally versatile, sulfate-reducing bacterium Desulfococcus multivorans DSM 2059

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Marvin Dörries ◽  
Lars Wöhlbrand ◽  
Michael Kube ◽  
Richard Reinhardt ◽  
Ralf Rabus
Keyword(s):  
Sulfate Reducing ◽  
Desulfococcus Multivorans

Anaerobically grown Thauera aromatica, Desulfococcus multivorans, Geobacter sulfurreducens are more sensitive towards organic solvents than aerobic bacteria

2007 ◽  
Vol 77 (3) ◽  
pp. 705-711 ◽  
Author(s):  
Ilka Duldhardt ◽  
Ivonne Nijenhuis ◽  
Frieder Schauer ◽  
Hermann J. Heipieper
Keyword(s):  
Organic Solvents ◽  
Geobacter Sulfurreducens ◽  
Aerobic Bacteria ◽  
Thauera Aromatica ◽  
Desulfococcus Multivorans

Anaerobic degradation of isobutyrate by methanogenic enrichment cultures and by a Desulfococcus multivorans strain

1989 ◽  
Vol 151 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Marion Stieb ◽  
Bernhard Schink
Keyword(s):  
Anaerobic Degradation ◽  
Enrichment Cultures ◽  
Desulfococcus Multivorans

The effect of temperature on sulfur and oxygen isotope fractionation by sulfate reducing bacteria (Desulfococcus multivorans)

2020 ◽  
Vol 367 (9) ◽  
Author(s):  
André Pellerin ◽  
Gilad Antler ◽  
Angeliki Marietou ◽  
Alexandra V Turchyn ◽  
Bo Barker Jørgensen
Keyword(s):  
Sulfate Reduction ◽  
Oxygen Isotope ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Sulfate Reducing Bacteria ◽  
Oxygen Isotope Fractionation ◽  
Sulfate Reducing ◽  
Sulfur Isotope Fractionation ◽  
Reducing Bacteria ◽  
Desulfococcus Multivorans

ABSTRACT Temperature influences microbiological growth and catabolic rates. Between 15 and 35 °C the growth rate and cell specific sulfate reduction rate of the sulfate reducing bacterium Desulfococcus multivorans increased with temperature. Sulfur isotope fractionation during sulfate reduction decreased with increasing temperature from 27.2 ‰ at 15 °C to 18.8 ‰ at 35 °C which is consistent with a decreasing reversibility of the metabolic pathway as the catabolic rate increases. Oxygen isotope fractionation, in contrast, decreased between 15 and 25 °C and then increased again between 25 and 35 °C, suggesting increasing reversibility in the first steps of the sulfate reducing pathway at higher temperatures. This points to a decoupling in the reversibility of sulfate reduction between the steps from the uptake of sulfate into the cell to the formation of sulfite, relative to the whole pathway from sulfate to sulfide. This observation is consistent with observations of increasing sulfur isotope fractionation when sulfate reducing bacteria are living near their upper temperature limit. The oxygen isotope decoupling may be a first signal of changing physiology as the bacteria cope with higher temperatures.

Sulfate transport in Desulfobulbus propionicus and Desulfococcus multivorans

1990 ◽  
Vol 154 (2) ◽  
pp. 144-149 ◽  
Author(s):  
Rolf Warthmann ◽  
Heribert Cypionka
Keyword(s):  
Sulfate Transport ◽  
Desulfobulbus Propionicus ◽  
Desulfococcus Multivorans
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