Nomenclature Abstract for Agrobacterium vitis Ophel and Kerr 1990.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
George M Garrity
Keyword(s):  
Agrobacterium Vitis

Exemplar Abstract for Agrobacterium vitis Ophel and Kerr 1990.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Agrobacterium Vitis

scholarly journals Isolation of a Gene Responsible for the Oxidation oftrans-Anethole topara-Anisaldehyde by Pseudomonas putida JYR-1 and Its Expression in Escherichia coli

2012 ◽  
Vol 78 (15) ◽  
pp. 5238-5246 ◽  
Author(s):  
Dongfei Han ◽  
Ji-Young Ryu ◽  
Robert A. Kanaly ◽  
Hor-Gil Hur
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Putida ◽  
Hypothetical Protein ◽  
Aldehyde Group ◽  
Agrobacterium Vitis ◽  
T7 Promoter ◽  
Content Type ◽  
E Coli ◽  
Cell Extracts ◽  
Isoeugenol Monooxygenase

ABSTRACTA plasmid, pTA163, inEscherichia colicontained an approximately 34-kb gene fragment fromPseudomonas putidaJYR-1 that included the genes responsible for the metabolism oftrans-anethole to protocatechuic acid. Three Tn5-disrupted open reading frame 10 (ORF 10) mutants of plasmid pTA163 lost their abilities to catalyzetrans-anethole. Heterologously expressed ORF 10 (1,047 nucleotides [nt]) under a T7 promoter inE. colicatalyzed oxidative cleavage of a propenyl group oftrans-anethole to an aldehyde group, resulting in the production ofpara-anisaldehyde, and this gene was designatedtao(trans-anetholeoxygenase). The deduced amino acid sequence of TAO had the highest identity (34%) to a hypothetical protein ofAgrobacterium vitisS4 and likely contained a flavin-binding site. Preferred incorporation of an oxygen molecule from water intop-anisaldehyde using18O-labeling experiments indicated stereo preference of TAO for hydrolysis of the epoxide group. Interestingly, unlike the narrow substrate range of isoeugenol monooxygenase fromPseudomonas putidaIE27 andPseudomonas nitroreducensJin1, TAO fromP. putidaJYR-1 catalyzed isoeugenol,O-methyl isoeugenol, and isosafrole, all of which contain the 2-propenyl functional group on the aromatic ring structure. Addition of NAD(P)H to the ultrafiltered cell extracts ofE. coli(pTA163) increased the activity of TAO. Due to the relaxed substrate range of TAO, it may be utilized for the production of various fragrance compounds from plant phenylpropanoids in the future.

scholarly journals LhnR and upstream operon LhnABC in Agrobacterium vitis regulate the induction of tobacco hypersensitive responses, grape necrosis and swarming motility

2012 ◽  
Vol 13 (7) ◽  
pp. 641-652 ◽  
Author(s):  
DESEN ZHENG ◽  
GUIXIA HAO ◽  
LUCIANA CURSINO ◽  
HONGSHENG ZHANG ◽  
THOMAS J. BURR
Keyword(s):  
Agrobacterium Vitis ◽  
Swarming Motility

scholarly journals The Presence and Characterization of a virF Gene on Agrobacterium vitis Ti Plasmids

1998 ◽  
Vol 11 (5) ◽  
pp. 429-433 ◽  
Author(s):  
B. Schrammeijer ◽  
J. Hemelaar ◽  
P. J. J. Hooykaas
Keyword(s):  
Amino Acids ◽  
Agrobacterium Tumefaciens ◽  
Molecular Mass ◽  
Promoter Region ◽  
Consensus Sequence ◽  
Tumor Formation ◽  
Nicotiana Glauca ◽  
Agrobacterium Vitis ◽  
Ti Plasmids

Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N. glauca a host plant for these strains, indicating that the VirF protein is a host-range determinant. Here we show the presence of a virF locus not only on the Agrobacterium vitis octopine/cucumopine plasmids pTiAg57 and pTiTm4, but also on the nopaline Ti plas-mids pTiAT1, pTiAT66a, and pTiAT66b. On the octopine Ti plasmids from A. tumefaciens the virF gene is located between the virE locus and the left border of the T-region. In contrast, the virF gene on Ti plasmids of A. vitis is located at the very left end of the vir-region near the virA locus. The virF gene of pTiAg57 has been sequenced and codes for a protein of 202 amino acids with a molecular mass of 22,280 Da. Comparison showed that the virF gene from A. vitis strain Ag57 is almost identical to that from A. tumefaciens octopine strains. The transcription of the pTiAg57 virF is inducible by the plant phenolic compound acetosyringone through the presence of a vir-box consensus sequence in its promoter region. The VirF protein from pTiAg57 can complement octopine A. tumefaciens strains deleted for virF as shown by tumor formation on N. glauca.

scholarly journals Bazı Üzüm Çeşit ve Amerikan Asma Anaçlarında Sıcak Su Uygulamasının Aşılı Çeliklerde Kallus Oluşumu Üzerine Etkileri

2020 ◽  
Vol 4 (2) ◽  
pp. 1-10
Author(s):  
Özlem ÇALKAN SAĞLAM ◽  
Hayri SAĞLAM
Keyword(s):  
Agrobacterium Vitis

Bu çalışmada, sağlıklı asma fidanı üretiminde Agrobacterium vitis eleminasyonu amacıyla yapılan sıcak su uygulamasının aşılı çeliklerde kallus oluşumuna etkisinin belirlenmesi amaçlanmıştır. Çalışmada 99R ve Ramsey anaçlarına ait aşılık çelikler ile Sultani Çekirdeksiz ve Yalova İncisi çeşitlerine ait kalemler materyal olarak kullanılmıştır. Aşılık çelik ve kalemler 50 oC de 15, 30 ve 45 dakikalık sıcak su uygulamaların ardından masabaşı omega aşılama yöntemi ile aşılanmıştır. Ramsey anacına Sultani Çekirdeksiz çeşidi, 99R anacına ise Sultani Çekirdeksiz ve Yalova İncisi çeşitleri aşılanmıştır. Bunun ardından aşılı çelikler standart aşılı fidan üretim programına alınarak kallus oluşumu durumu değerlendirilmiştir. Sıcak su uygulaması yapıldıktan sonra 21 gün gelişmeye bırakılmış aşılı çeliklerde kallus oluşumları değerlendirilmiştir. Kombinasyonlar arasında önemli farklar olduğu belirlenmiştir. Sıcak su uygulamasında kallus oluşturma durumu uygulama süresinden çok anaç ve çeşide bağlı görünmektedir.

scholarly journals Crown Gall of Grape: Biology of Agrobacterium vitis and the Development of Disease Control Strategies

Plant Disease ◽  
1998 ◽  
Vol 82 (12) ◽  
pp. 1288-1297 ◽  
Author(s):  
Thomas J. Burr ◽  
Carlo Bazzi ◽  
Sandor Süle ◽  
Leon Otten
Keyword(s):  
Disease Control ◽  
Crown Gall ◽  
Control Strategies ◽  
Agrobacterium Vitis

scholarly journals Regulation of Long-Chain N-Acyl-Homoserine Lactones in Agrobacterium vitis

2006 ◽  
Vol 188 (6) ◽  
pp. 2173-2183 ◽  
Author(s):  
Guixia Hao ◽  
Thomas J. Burr
Keyword(s):  
Sinorhizobium Meliloti ◽  
Site Directed Mutagenesis ◽  
Agrobacterium Vitis ◽  
Fusion Construct ◽  
Homoserine Lactones ◽  
Dna Binding Domains ◽  
Binding Domains ◽  
Acyl Homoserine Lactones ◽  
High Level ◽  
Long Chain

ABSTRACT Homologs of quorum-sensing luxR and luxI regulatory genes, avsR and avsI, were identified in Agrobacterium vitis strain F2/5. Compared to other LuxI proteins from related species, the deduced AvsI shows the greatest identity to SinI (71%) from Sinorhizobium meliloti Rm1021. AvsR possesses characteristic autoinducer binding and helix-turn-helix DNA binding domains and shares a high level of identity with SinR (38%) from Rm1021. Site-directed mutagenesis of avsR and avsI was performed, and both genes are essential for hypersensitive-like response (HR) and necrosis. Two hypothetical proteins (ORF1 and ORF2) that are positioned downstream of avsR-avsI are also essential for the phenotypes. Profiles of N-acyl-homoserine lactones (AHLs) isolated from the wild type and mutants revealed that disruption of avsI, ORF1, or ORF2 abolished the production of long-chain AHLs. Disruption of avsR reduces long-chain AHLs. Expression of a cloned avsI gene in A. tumefaciens strain NT1 resulted in synthesis of long-chain AHLs. The necrosis and HR phenotypes of the avsI and avsR mutants were fully complemented with cloned avsI. The addition of synthetic AHLs (C16:1 and 3-O-C16:1) complemented grape necrosis in the avsR, avsI, ORF1, and ORF2 mutants. It was determined by reverse transcriptase PCR that the expression level of avsI is regulated by avsR but not by aviR or avhR, two other luxR homologs which were previously shown to be associated with induction of a tobacco hypersensitive response and grape necrosis. We further verified that avsR regulates avsI by measuring the expression of an avsI::lacZ fusion construct.

scholarly journals Grapevine (Vitis vinifera) Crown Galls Host Distinct Microbiota

2016 ◽  
Vol 82 (18) ◽  
pp. 5542-5552 ◽  
Author(s):  
Hanna Faist ◽  
Alexander Keller ◽  
Ute Hentschel ◽  
Rosalia Deeken
Keyword(s):  
Microbial Community ◽  
Crown Gall ◽  
Amplicon Sequencing ◽  
Agrobacterium Vitis ◽  
Graft Union ◽  
Content Type ◽  
Crown Gall Tumor ◽  
Crown Gall Disease ◽  
Opportunistic Bacteria

ABSTRACTCrown gall disease of grapevine is caused by virulentAgrobacteriumstrains and establishes a suitable habitat for agrobacteria and, potentially, other bacteria. The microbial community associated with grapevine plants has not been investigated with respect to this disease, which frequently results in monetary losses. This study compares the endophytic microbiota of organs from grapevine plants with or without crown gall disease and the surrounding vineyard soil over the growing seasons of 1 year. Amplicon-based community profiling revealed that the dominating factor causing differences between the grapevine microbiota is the sample site, not the crown gall disease. The soil showed the highest microbial diversity, which decreased with the distance from the soil over the root and the graft union of the trunk to the cane. Only the graft union microbiota was significantly affected by crown gall disease. The bacterial community of graft unions without a crown gall hosted transient microbiota, with the three most abundant bacterial species changing from season to season. In contrast, graft unions with a crown gall had a higher species richness, which in every season was dominated by the same three bacteria (Pseudomonassp.,Enterobacteriaceaesp., andAgrobacterium vitis). Forin vitro-cultivated grapevine plantlets,A. vitisinfection alone was sufficient to cause crown gall disease. Our data show that microbiota in crown galls is more stable over time than microbiota in healthy graft unions and that the microbial community is not essential for crown gall disease outbreak.IMPORTANCEThe characterization of bacterial populations in animal and human diseases using high-throughput deep-sequencing technologies, such as 16S amplicon sequencing, will ideally result in the identification of disease-specific microbiota. We analyzed the microbiota of the crown gall disease of grapevine, which is caused by infection with the bacterial pathogenAgrobacterium vitis.All otherAgrobacteriumspecies were found to be avirulent, even though they lived together withA. vitisin the same crown gall tumor. As has been reported for human cancer, the crown gall tumor also hosted opportunistic bacteria that are adapted to the tumor microenvironment. Characterization of the microbiota in various diseases using amplicon sequencing may help in early diagnosis, to serve as a preventative measure of disease in the future.

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