Lentiviral-Mediated HoxB4 Expression in Human Embryonic Stem Cells Initiates Early Hematopoiesis in a Dose-Dependent Manner but Does Not Promote Myeloid Differentiation

Stem Cells ◽  
2008 ◽  
Vol 26 (10) ◽  
pp. 2455-2466 ◽  
Author(s):  
Christian Unger ◽  
Elerin Kärner ◽  
Alexandra Treschow ◽  
Birgitta Stellan ◽  
Ulrika Felldin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Myeloid Differentiation ◽  
Dependent Manner ◽  
Dose Dependent

304 THE DEVELOPMENTAL TOXICITY EVALUATION OF 5-FLUOROURACIL AND INDOMETHACIN IN HUMAN EMBRYONIC STEM CELLS

2013 ◽  
Vol 25 (1) ◽  
pp. 299
Author(s):  
E. M. Jung ◽  
E. B. Jeung
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Developmental Process ◽  
Developmental Toxicity ◽  
Embryonic Stem ◽  
Surface Markers ◽  
Dependent Manner ◽  
Undifferentiated Hesc

Embryonic stem cells have pluripotency and differentiate into and constitute the cells and tissues of our body. In this study, using human embryonic stem cells (hESC), we evaluated novel methods for screening toxicological chemicals during developmental process. We elucidated developmental toxicity of two well-known chemicals, 5-fluorouracil (5-FU) and indomethacin (Indo) in hESC. The undifferentiated hESC were treated with the chemicals (10–4 to 104 µM of 5-FU and Indo) in a dose-dependent manner during 1 to 3 days. Surface markers (SSEA-4, TRA-1-60, and TRA-1-81) expressed only in undifferentiated hESC were monitored by immunocytochemistry to ensure the characterisation of undifferentiated hESC. Moreover, expression of embryonic stem cell-specific genes was assessed with real-time PCR after treatment of 5-FU and Indo (10–2, 100, and102 µM of 5-FU and Indo). The expression of surface markers was not significantly affected by treatment of 5-FU and Indo. The expression of transcription factors (Oct-4, Sox-2, Nanog, and hTERT) was significantly decreased by high concentrations of 5-FU and Indo (102 µM). However, no difference was observed in treatment of low concentration of 5-FU and Indo (10–2 µM). Taken together, these results suggest that 5-FU and Indo have cytotoxic effects, and modulate the expression of transcription factors that have pivotal roles in undifferentiated hESC. Therefore, we suggest that hESC may have potential to test toxicity of chemicals during embryonic developmental stage.

scholarly journals Immunity-and-Matrix-Regulatory Cells Derived from Human Embryonic Stem Cells Safely and Effectively Treat Mouse Lung Injury and Fibrosis

2020 ◽  
Author(s):  
Jun Wu ◽  
Dingyun Song ◽  
Zhongwen Li ◽  
Baojie Guo ◽  
Yani Xiao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Lung Injury ◽  
Human Embryonic Stem Cells ◽  
Pulmonary Inflammation ◽  
Embryonic Stem ◽  
Mouse Lung ◽  
Dependent Manner ◽  
Regulatory Cells

AbstractLung injury and fibrosis represent the most significant outcomes of severe and acute lung disorders, including COVID-19. However, there are still no effective drugs to treat lung injury and fibrosis. In this study, we report the generation of clinical-grade human embryonic stem cells (hESCs)-derived immunity- and matrix-regulatory cells (IMRCs) produced under good manufacturing practice (GMP) requirements, that can treat lung injury and fibrosis in vivo. We generate IMRCs by sequentially differentiating hESCs with serum-free reagents. IMRCs possess a unique gene expression profile distinct from umbilical cord mesenchymal stem cells (UCMSCs), such as higher levels of proliferative, immunomodulatory and anti-fibrotic genes. Moreover, intravenous delivery of IMRCs inhibits both pulmonary inflammation and fibrosis in mouse models of lung injury, and significantly improves the survival rate of the recipient mice in a dose-dependent manner, likely through paracrine regulatory mechanisms. IMRCs are superior to both primary UCMSCs and FDA-approved pirfenidone, with an excellent efficacy and safety profile in mice and monkeys. In light of public health crises involving pneumonia, acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), our findings suggest that IMRCs are ready for clinical trials on lung disorders.

Differentiation of human embryonic stem cells into osteogenic or hematopoietic lineages: A dose-dependent effect of osterix over-expression

2009 ◽  
Vol 218 (2) ◽  
pp. 323-333 ◽  
Author(s):  
Elerin Kärner ◽  
Christian Unger ◽  
Radim Cerny ◽  
Lars Ährlund-Richter ◽  
Bernhard Ganss ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Dependent Effect ◽  
Over Expression ◽  
Dose Dependent ◽  
Dose Dependent Effect

scholarly journals Transgenes Delivered by Lentiviral Vector are Suppressed in Human Embryonic Stem Cells in A Promoter-Dependent Manner

2007 ◽  
Vol 16 (1) ◽  
pp. 167-176 ◽  
Author(s):  
Xiaofeng Xia ◽  
Yingsha Zhang ◽  
Caroline R. Zieth ◽  
Su-Chun Zhang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Lentiviral Vector ◽  
Embryonic Stem ◽  
Dependent Manner

scholarly journals Assessment of Toxic Effects of Ochratoxin A in Human Embryonic Stem Cells

Toxins ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 217 ◽  
Author(s):  
Slaven Erceg ◽  
Eva Mateo ◽  
Iván Zipancic ◽  
Francisco Rodríguez Jiménez ◽  
María Pérez Aragó ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Ochratoxin A ◽  
Human Embryonic Stem Cells ◽  
Cell Attachment ◽  
Animal Feed ◽  
Embryonic Stem ◽  
Toxic Effects ◽  
Dependent Manner ◽  
Toxicological Studies

Ochratoxin A (OTA) is a mycotoxin produced by different Aspergillus and Penicillium species, and it is considered a common contaminant in food and animal feed worldwide. On the other hand, human embryonic stem cells (hESCs) have been suggested as a valuable model for evaluating drug embryotoxicity. In this study, we have evaluated potentially toxic effects of OTA in hESCs. By using in vitro culture techniques, specific cellular markers, and molecular biology procedures, we found that OTA produces mild cytotoxic effects in hESCs by inhibiting cell attachment, survival, and proliferation in a dose-dependent manner. Thus, we suggest that hESCs provide a valuable human and cellular model for toxicological studies regarding preimplantation stage of human fetal development.

Silver nanoparticles exhibit coating and dose-dependent neurotoxicity in glutamatergic neurons derived from human embryonic stem cells

2016 ◽  
Vol 57 ◽  
pp. 45-53 ◽  
Author(s):  
Aynun N. Begum ◽  
Jose S. Aguilar ◽  
Lourdes Elias ◽  
Yiling Hong
Keyword(s):  
Stem Cells ◽  
Silver Nanoparticles ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Glutamatergic Neurons ◽  
Dose Dependent

356 Human embryonic stem cells improve diastolic function in rats with acute myocardial infarction

2007 ◽  
Vol 6 (1) ◽  
pp. 82-82
Author(s):  
M LINDBOM ◽  
T RAMUNDDAL ◽  
E BOLLANO ◽  
E OMEROVIC ◽  
M SHARINTANG
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Diastolic Function ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression
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