EFFECT OF CORTICOTROPHIN AND CORTICOSTEROIDS ON PLASMA INTERSTITIAL CELL-STIMULATING HORMONE AND URINARY STEROIDS IN THE BOAR

SUMMARY Levels of interstitial cell-stimulating hormone (ICSH) activity in plasma estimated by the rat ovarian cholesterol depletion method and urinary gonadal steroid excretion were studied in boars under various experimental conditions. Injections of adrenocorticotrophic hormone (ACTH), hydrocortisone and prednisolone trimethylacetate were found to lower the level of plasma ICSH and urinary dehydroepiandrosterone (DHA) and oestrogens. This action of ACTH is thought to be mediated through the adrenal cortex since ACTH did not reduce urinary DHA or plasma ICSH activity in the adrenalectomized boar. Hydrocortisone reduced ICSH activity in the castrated boar. When hydrocortisone was administered at the same time as testicular steroidogenesis was stimulated by chorionic gonadotrophin in young hypophysectomized or intact boars, no pronounced influence was found on their elevated DHA excretion. It is concluded that in the boar some adrenal corticosteroids are capable of depressing plasma ICSH activity which results in reduced testicular steroidogenesis.

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LACK OF AROMATISATION OF CIRCULATING DEHYDROEPIANDROSTERONE AND DEHYDROEPIANDROSTERONE SULPHATE IN AMENORRHOEIC WOMEN STIMULATED WITH HUMAN GONADOTROPHINS

Acta Endocrinologica ◽

10.1530/acta.0.0490248 ◽

1965 ◽

Vol 49 (2) ◽

pp. 248-261 ◽

Cited By ~ 8

Author(s):

S. Mancuso ◽

Francesca P. Mancuso ◽

K.-G. Tillinger ◽

E. Diczfalusy

Keyword(s):

Pregnant Women ◽

Ovarian Stimulation ◽

Interstitial Cell ◽

Follicular Phase ◽

Radioactive Material ◽

Dehydroepiandrosterone Sulphate ◽

Experimental Conditions ◽

Human Menopausal Gonadotrophin ◽

Urine Specimens ◽

Stimulating Hormone

ABSTRACT Two amenorrhoeic women were given a course of 10 injections of human menopausal gonadotrophin (HMG) in daily doses corresponding to 260 IU of follicle stimulating hormone (FSH) activity and 165 IU of interstitial cell stimulating hormone (ICSH) activity. In both patients an extensive ovarian stimulation was observed as indicated by the greatly increased urinary excretion of oestrone, 17β-oestradiol and oestriol. When HMG-treatment was followed subsequently by the administration of human chorionic gonadotrophin (HCG) for 5 days in a total dose of 18 000 and 30 000 IU, respectively, functional corpus luteum tissue was formed in both patients as evidenced by a huge rise in urinary pregnane-3α,20α-diol excretion and by the secretory transformation of a previously atrophic endometrium. At the approximate height of the follicular phase tracer doses of 3H-labelled dehydroepiandrosterone sulphate (DHAS) and 14C-labelled dehydroepiandrosterone (DHA) were administered to both patients in the form of a continuous intravenous infusion of 10 hours' duration. Infusion of the same dose was repeated under identical experimental conditions at the approximate height of the luteal phase. In both patients, very little radioactive material was associated with oestrone and 17β-oestradiol and none with oestriol isolated from 96-hours' urine specimens obtained at both phases of ovarian stimulation. It is concluded that — in contradistinction to the situation in pregnant women — circulating DHAS is not a significant precursor of urinary oestrogens in non-pregnant women.

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A STUDY OF THE ANTIBODIES PRODUCED IN RESPONSE TO PURIFIED PREPARATIONS OF SHEEP INTERSTITIAL CELL STIMULATING HORMONE

Journal of Endocrinology ◽

10.1677/joe.0.0160310 ◽

1958 ◽

Vol 16 (3) ◽

pp. 310-325 ◽

Cited By ~ 9

Author(s):

SARAH S. HENRY ◽

H. B. van DYKE

Keyword(s):

Biological Effect ◽

Interstitial Cell ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Cross Reaction ◽

Antigen Antibody ◽

Stimulating Hormone

SUMMARY 1. The antibodies produced in rabbits in response to purified preparations of sheep interstitial cell stimulating hormone (ICSH) have been studied by the Ouchterlony technique for the analysis of precipitins. It has been possible to identify the ICSH precipitin in vitro. 2. The antibody to sheep ICSH forms precipitin bands with sheep ICSH and with ox ICSH, but not with hog ICSH or with human chorionic gonadotrophin (HCG). 3. Antiserum to sheep ICSH, absorbed so that a single demonstrable antibody is present, inhibits the biological effect of sheep and of ox ICSH but not of hog ICSH or of HCG. This antiserum did not interfere with the action of endogenous rat ICSH. 4. There was no cross-reaction demonstrable between the sheep ICSH antigen-antibody system and the pneumococcus polysaccharide type XIV system as has been reported for the antigen-antibody system of HCG. 5. Two attempts to repeat the method of Takeda, Otsuka & Noda [1952] for the preparation of crystalline ox ICSH were unsuccessful.

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URINARY STEROID EXCRETION BY THE SQUIRREL MONKEY (SAIMURI SCIUREUS)

Journal of Endocrinology ◽

10.1677/joe.0.0730365 ◽

1977 ◽

Vol 73 (2) ◽

pp. 365-375 ◽

Cited By ~ 17

Author(s):

K. D. R. SETCHELL ◽

K. S. CHUA ◽

R. L. HIMSWORTH

Keyword(s):

Squirrel Monkey ◽

Urinary Free Cortisol ◽

Complete Suppression ◽

Adrenocorticotrophic Hormone ◽

Conspicuous Feature ◽

Urinary Steroids ◽

Free Cortisol ◽

Steroid Excretion ◽

Urinary Steroid ◽

Steroid Conjugates

SUMMARY Urinary steroids and steroid conjugates were measured in the squirrel monkey (Saimuri sciureus). The principal steroids excreted were cortisol, 11β,17α,20β,21-tetrahydroxy-4pregnen-3-one (20β-dihydrocortisol), 11β,17α,20α,21-tetrahydroxy-4-pregnen-3-one (20α-dihydrocortisol), α- and β-cortol and α- and β-cortolone. The majority of the steroids were excreted unconjugated and a conspicuous feature of the pattern was the large amount of urinary free cortisol. Unlike man there was an insignificant excretion of 3α,17α,21-trihydroxy-5β-pregnane-11,20-dione (tetrahydrocortisone) and 3α,11β,17α,21-tetrahydroxy-5β-pregnan-20-one (tetrahydrocortisol). A steroid not previously identified in urine from any species was one of the major glucuronide conjugates; it was characterized as having the structure 3β,17α,20ξ,21-tetrahydroxy-5β-pregnan-11-one. Administration of dexamethasone resulted in complete suppression of steroid output, whilst the response to adrenocorticotrophic hormone was inconstant.

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STANDARDIZATION OF THE BIOLOGICAL ACTIVITY OF HUMAN PITUITARY INTERSTITIAL CELL-STIMULATING HORMONE IN TERMS OF HUMAN CHORIONIC GONADOTROPHIN

Journal of Endocrinology ◽

10.1677/joe.0.0260215 ◽

1963 ◽

Vol 26 (2) ◽

pp. 215-218 ◽

Cited By ~ 5

Author(s):

A. J. LOSTROH ◽

P. G. SQUIRE ◽

C. H. LI

Keyword(s):

Biological Activity ◽

Dose Response ◽

Interstitial Cell ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Female Rats ◽

Male Rats ◽

Ventral Prostate ◽

Flat Dose ◽

Stimulating Hormone

SUMMARY The biological activity of purified human interstitial cell-stimulating hormone (ICSH) has been standardized in terms of human chorionic gonadotrophin (HCG), by means of the ventral prostate test in hypophysectomized male rats and by means of the interstitial cell repair test in hypophysectomized female rats. In the ventral prostate assay, human ICSH injected in saline, unlike HCG, gave a very flat dose-response curve; injected in 16% gelatin, the two hormones gave dose-response curves that were indistinguishable from each other; the estimated potency of the ICSH preparation was equivalent to 500 i.u./mg. In the interstitial repair test, a total dose of 1·4 μg. of human ICSH effected repair similar to that obtained with 0·7 i.u. of HCG, i.e. 1 mg. of ICSH was equivalent to 500 i.u. of HCG. Thus, the potency of purified human ICSH can be estimated in terms of HCG by either of the two assays, and comparisons between different ICSH preparations can be made on this basis.

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