A STUDY OF THE ANTIBODIES PRODUCED IN RESPONSE TO PURIFIED PREPARATIONS OF SHEEP INTERSTITIAL CELL STIMULATING HORMONE

1958 ◽  
Vol 16 (3) ◽  
pp. 310-325 ◽  
Author(s):  
SARAH S. HENRY ◽  
H. B. van DYKE
Keyword(s):  
Biological Effect ◽  
Interstitial Cell ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Cross Reaction ◽  
Antigen Antibody ◽  
Stimulating Hormone

SUMMARY 1. The antibodies produced in rabbits in response to purified preparations of sheep interstitial cell stimulating hormone (ICSH) have been studied by the Ouchterlony technique for the analysis of precipitins. It has been possible to identify the ICSH precipitin in vitro. 2. The antibody to sheep ICSH forms precipitin bands with sheep ICSH and with ox ICSH, but not with hog ICSH or with human chorionic gonadotrophin (HCG). 3. Antiserum to sheep ICSH, absorbed so that a single demonstrable antibody is present, inhibits the biological effect of sheep and of ox ICSH but not of hog ICSH or of HCG. This antiserum did not interfere with the action of endogenous rat ICSH. 4. There was no cross-reaction demonstrable between the sheep ICSH antigen-antibody system and the pneumococcus polysaccharide type XIV system as has been reported for the antigen-antibody system of HCG. 5. Two attempts to repeat the method of Takeda, Otsuka & Noda [1952] for the preparation of crystalline ox ICSH were unsuccessful.

345 EFFECT OF FOLLICLE STIMULATING HORMONE AND HUMAN CHORIONIC GONADOTROPHIN ON THE IN VITRO MATURATION OF CANINE OOCYTES

2007 ◽  
Vol 19 (1) ◽  
pp. 288
Author(s):  
M.-K. Kim ◽  
H.-J. Oh ◽  
Y. H. Fibrianto ◽  
G. Jang ◽  
H.-J. Kim ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Uv Light ◽  
Follicle Stimulating Hormone ◽  
Reproductive Hormones ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Nuclear Maturation ◽  
Maturation Rate ◽  
Stimulating Hormone

Growth, maturation, and ovulation of the Graafian follicle depend on appropriate patterns of secretion, sufficient concentrations, and adequate ratios of various reproductive hormones, especially follicle stimulating hormone (FSH) and luteinizing hormone (LH) (Van Tol et al. 1996 Mol. Reprod. Dev. 45, 218–224). The present study investigated the effects of FSH and human chorionic gonadotrophin (hCG) on the nuclear maturation of canine oocytes. In addition, in order to investigate the effect of stage of the estrous cycle on the meiotic competence of canine oocytes matured in vitro, oocytes were collected from various reproductive states and matured in vitro in the presence of the gonadotrophins. Estrous cycle stage was evaluated for each bitch by ovarian morphology, and bitches were categorized according to the stage of the estrous cycle (anestrus, follicular, or diestrus) prior to oocyte collection. Recovered oocytes were cultured in serum-free tissue culture medium (TCM)-199 supplemented with various concentrations of FSH (Exp. 1: 0, 0.1, 1.0, or 10 IU) or hCG (Exp. 2: 0, 0.5, 1.0, or 10 IU) or both (Exp. 3: 1 IU FSH + 1 IU hCG) for 72 h to determine the effective concentration of these hormones, and to examine their combined effect. After maturation culture, oocytes were denuded in PBS containing 0.1% (w/v) hyaluronidase by gentle pipetting. The denuded oocytes were stained with Hoechst 33342 in glycerol and the nuclear state of oocytes was evaluated under UV light. The rates of maturation to the MII stage were significantly higher (P < 0.05) when follicular-stage oocytes were supplemented with 1 IU FSH (6.2%) compared with the other FSH-supplemented groups (0.0 to 3.3%) or to the control (1.8%), or 0.1 or 10.0 IU FSH (0 to 1.2%). Significantly higher (P < 0.05) maturation rate to MII stage was observed in follicular-stage oocytes supplemented with 1.0 IU hCG (7.2%) compared with the control or other hCG-supplemented groups (0 to 1.5%). However, FSH and hCG together did not improve the nuclear maturation rate of canine oocytes (2.4%) compared with FSH (6.2%) and hCG alone (7.2%). In conclusion, FSH or hCG alone significantly increased the maturation of canine oocytes to the MII stage. This work was supported by grant No. M1062503005-06N250300510 from KOSEF, Republic of Korea.

ISOLATION OF RAT LEYDIG CELLS BY DENSITY GRADIENT CENTRIFUGATION

1982 ◽  
Vol 92 (2) ◽  
pp. 293-NP ◽  
Author(s):  
J. S. GALE ◽  
J. ST J. WAKEFIELD ◽  
H. C. FORD
Keyword(s):  
Density Gradient ◽  
Binding Sites ◽  
Leydig Cells ◽  
Interstitial Cell ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Collagenase Treatment

A rapid method for preparing Leydig cells from rat testes is described. An interstitial cell suspension, prepared by collagenase treatment of decapsulated testes, was centrifuged for 10 min over a cushion of 60% (v/v) Percoll to remove red blood cells, and then centrifuged for 20 min in a 0–60% linear density gradient of Percoll. Seventy-four per cent of the cells present in that fraction of the gradient comprising 35–50% Percoll were Leydig cells; the yield from each testis was about 1·5 × 106 cells. The Leydig cells appeared viable, excluded Trypan blue, possessed high-affinity binding sites for human chorionic gonadotrophin (hCG) and synthesized increased quantities of testosterone in response to hCG. The cells could be stored overnight in 20% (v/v) glycerol at −20 °C, with only minimal effect on the specific activities of a number of enzymes used as markers of subcellular components. Testosterone production in vitro by the cells after storage for 20 h was greater than that of hCG-stimulated fresh cells and was not further increased by hCG.

THE ACTION OF FOLLICLE STIMULATING HORMONE AND OF HUMAN CHORIONIC GONADOTROPHIN UPON STEROID SYNTHESIS BY RABBIT OVARIAN TISSUES IN VITRO

1964 ◽  
Vol 47 (2) ◽  
pp. 293-305 ◽  
Author(s):  
Denis Gospodarowicz
Keyword(s):  
Metabolic Pathways ◽  
Specific Activity ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Steroid Synthesis ◽  
Site Of Action ◽  
Metabolic Sequence ◽  
Stimulating Hormone

ABSTRACT Rabbit ovarian follicles have been incubated with sodium acetate-14C with and without the presence of gonadotrophin. The major radioactive metabolites found were dehydroepiandrosterone (DHEA), androst-5-ene-3β,17β-diol, testosterone, and 17α-oestradiol. Lesser amounts of radioactivity were found in pregnenolone, progesterone, androstenedione, and oestrone. The results are interpreted as evidence that two metabolic pathways exist in the follicle; the major one giving rise to androgens and oestrogens from Δ5-3β-hydroxysteroids while the other pathway involves pregnenolone and progesterone as intermediates. Follicle stimulating hormone (FSH) and human chorionic gonadotrophin (HCG) both increased the incorporation of acetate-14C into steroids and decreased the specific activity of cholesterol. The site of action of the gonadotrophins in the metabolic sequence is discussed.

TESTICULAR ANDROGEN BIOSYNTHESIS FOLLOWING CORTICOTROPHIN AND HUMAN CHORIONIC GONADOTROPHIN ADMINISTRATION

1965 ◽  
Vol 50 (3) ◽  
pp. 365-378 ◽  
Author(s):  
Edgar J. Schoen ◽  
Leo T. Samuels
Keyword(s):  
Interstitial Cell ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Steroid Biosynthesis ◽  
Clear Cut ◽  
Adrenocortical Hyperplasia ◽  
Testicular Interstitial Cells ◽  
Stimulation Of

ABSTRACT The effects of in vivo administration of corticotrophin (ACTH) and human chorionic gonadotrophin (HCG) on rat testes were studied. HCG, administered for 14 days to young rats, led to testicular interstitial cell hypertrophy and hyperplasia, accompanied by stimulation of in vitro testicular androgen production, as manifested by increased 17α-hydroxylase and 17β-desmolase enzyme activities, with no clear-cut effect on 20α-hydroxysteroid dehydrogenase activity. The findings in ACTH-treated animals were similar to those in the saline-treated controls. The lack of influence of ACTH on the testicular enzymes involved in steroid biosynthesis is consonant with the concept that ACTH-dependent bilateral testicular tumours occurring in boys with congenital adrenocortical hyperplasia arise from »adrenocortical rest cells« rather than from testicular interstitial cells.

STANDARDIZATION OF THE BIOLOGICAL ACTIVITY OF HUMAN PITUITARY INTERSTITIAL CELL-STIMULATING HORMONE IN TERMS OF HUMAN CHORIONIC GONADOTROPHIN

1963 ◽  
Vol 26 (2) ◽  
pp. 215-218 ◽  
Author(s):  
A. J. LOSTROH ◽  
P. G. SQUIRE ◽  
C. H. LI
Keyword(s):  
Biological Activity ◽  
Dose Response ◽  
Interstitial Cell ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
Male Rats ◽  
Ventral Prostate ◽  
Flat Dose ◽  
Stimulating Hormone

SUMMARY The biological activity of purified human interstitial cell-stimulating hormone (ICSH) has been standardized in terms of human chorionic gonadotrophin (HCG), by means of the ventral prostate test in hypophysectomized male rats and by means of the interstitial cell repair test in hypophysectomized female rats. In the ventral prostate assay, human ICSH injected in saline, unlike HCG, gave a very flat dose-response curve; injected in 16% gelatin, the two hormones gave dose-response curves that were indistinguishable from each other; the estimated potency of the ICSH preparation was equivalent to 500 i.u./mg. In the interstitial repair test, a total dose of 1·4 μg. of human ICSH effected repair similar to that obtained with 0·7 i.u. of HCG, i.e. 1 mg. of ICSH was equivalent to 500 i.u. of HCG. Thus, the potency of purified human ICSH can be estimated in terms of HCG by either of the two assays, and comparisons between different ICSH preparations can be made on this basis.

STUDIES ON THE SPECIFICITY OF THE RADIOIMMUNOASSAY OF LUTEINISING HORMONE

1970 ◽  
Vol 63 (3) ◽  
pp. 545-561 ◽  
Author(s):  
J. M. H. Buckler ◽  
Barbara E. Clayton
Keyword(s):  
Luteinising Hormone ◽  
Relative Concentration ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Cross Reaction ◽  
The Cross ◽  
Stimulating Hormone

ABSTRACT The cross reaction of follicle stimulating hormone (FSH) with antisera raised to human chorionic gonadotrophin (HCG) for use in the radioimmunoassay of luteinising hormone (LH) has been found to be influenced by many factors. These include the relative concentration of the hormones, the concentration of antiserum, the length of the assay stages, and the particular preparation of FSH. Antisera do not react identically with HCG and LH. This effect varies from antiserum to antiserum and so does the ability to detect the immunological differences which become apparent between different preparations of FSH and between hormones of urinary and pituitary origin. These findings are of considerable relevance to the assessment of the validity of a radioimmunoassay system.

STIMULATION BY HUMAN CHORIONIC GONADOTROPHIN OF OESTRADIOL PRODUCTION BY DISPERSED CELLS FROM HUMAN CORPUS LUTEUM: COMPARISON WITH PROGESTERONE PRODUCTION; UTILIZATION OF EXOGENOUS TESTOSTERONE

1981 ◽  
Vol 91 (2) ◽  
pp. 197-203 ◽  
Author(s):  
M. C. RICHARDSON ◽  
G. M. MASSON
Keyword(s):  
Luteal Phase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Cell Suspensions ◽  
Corpora Lutea ◽  
Progesterone Production ◽  
Late Luteal Phase ◽  
Oestradiol Production ◽  
Dispersed Cells

Cell suspensions were prepared from tissue samples of human corpora lutea obtained during the mid- and late-luteal phase of the menstrual cycle. Both oestradiol and progesterone production by dispersed cells were stimulated by similar concentrations of human chorionic gonadotrophin (hCG). As the degree of stimulation of production by hCG was greater for progesterone than for oestradiol (five- to tenfold compared with two- to threefold higher than basal production), the ratio of progesterone to oestradiol produced varied according to the level of trophic stimulation. A comparison of cell suspensions prepared from mid- and late-luteal phase corpora lutea, exposed to the same concentration of hCG (10 i.u./ml) in vitro, did not reveal a shift to oestradiol production in the late-luteal phase. Provision of additional testosterone during incubation raised the level of oestradiol production by dispersed luteal cells. At an optimum concentration of testosterone (1 μmol/l), oestradiol synthesis was not raised further in the presence of hCG or N6, O2-dibutyryl cyclic AMP, suggesting a lack of induction or activation of the aromatase system by gonadotrophin in short-term cultures. Basal and stimulated levels of progesterone production were not significantly impaired in the presence of testosterone.

Sign in / Sign up

Export Citation Format

Share Document