AUTORADIOGRAPHIC STUDY OF LYSINE AND ARGININE INCORPORATION STIMULATED BY FOLLICLE-STIMULATING HORMONE IN THE MOUSE TESTIS IN VIVO

Hypophysectomized adult mice were given injections of highly purified FSH 12 h before killing, and of tritiated lysine or arginine 2 h before killing. Autoradiographs were prepared from paraffin wax sections of testes. Treatment with FSH increased the density of silver grains over the nuclei of the Sertoli cells and of all types of germinal cell except the early spermatids. The stimulatory effect of FSH on incorporation of both lysine and arginine was most marked in the nuclei of preleptotene primary spermatocytes. The ratio of arginine to lysine incorporation was greater in spermatids undergoing nuclear elongation than in other types of cell.

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TIMING AND LOCALIZATION OF THE STIMULATORY EFFECT OF FOLLICLE-STIMULATING HORMONE ON URIDINE INCORPORATION IN THE MOUSE TESTIS IN VIVO

Journal of Endocrinology ◽

10.1677/joe.0.0880443 ◽

1981 ◽

Vol 88 (3) ◽

pp. 443-449 ◽

Cited By ~ 1

Author(s):

A. G. DAVIES ◽

N. R. LAWRENCE

Keyword(s):

Sertoli Cells ◽

Rna Synthesis ◽

Germinal Cell ◽

Human Pituitary ◽

Stimulatory Action ◽

Subcutaneous Injections ◽

Adult Mice ◽

Old Mice ◽

Silver Grains

Human pituitary FSH increased the incorporation of [5-3H]uridine into RNA in vivo in the testes of intact 9-day-old mice and of hypophysectomized adults. In both groups the effect was greatest 8 h after administration of 5–7·5 i.u. FSH. Autoradiographs were prepared from the testes of hypophysectomized adult mice given subcutaneous injections of FSH or of 0·9% saline 6 h, and [5-3H]uridine 1·5 h, before death. Treatment with FSH caused statistically significant increases in the density of silver grains over the nuclei of Sertoli cells, type A and intermediate spermatogonia, and preleptotene and mid-pachytene primary spermatocytes. It was concluded that FSH has a generalized stimulatory action on RNA synthesis in the nuclei of Sertoli cells and those types of germinal cell which synthesize RNA most actively.

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Autoradiographic Study of Binding and Internalization of Follicle-Stimulating Hormone in the Mouse Testis Minces In Vitro

Endocrinologia Japonica ◽

10.1507/endocrj1954.34.431 ◽

1987 ◽

Vol 34 (3) ◽

pp. 431-442 ◽

Cited By ~ 11

Author(s):

AKITOSHI SHIMIZU ◽

KAZUYOSHI TSUTSUI ◽

SEIICHIRO KAWASHIMA

Keyword(s):

Follicle Stimulating Hormone ◽

Autoradiographic Study ◽

Mouse Testis ◽

Stimulating Hormone

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Manipulating the In Vivo mRNA Expression Profile of FSHbeta to Resemble that of LHbeta Does Not Promote a Concomitant Increase in Intracellular Storage of Follicle-Stimulating Hormone

Journal of Neuroendocrinology ◽

10.1046/j.1365-2826.2001.00594.x ◽

2001 ◽

Vol 13 (1) ◽

pp. 50-62 ◽

Cited By ~ 7

Author(s):

P. Brown ◽

J. R. McNeilly ◽

J. G. Evans ◽

G. M. Crawford ◽

M. Walker ◽

...

Keyword(s):

Mrna Expression ◽

Expression Profile ◽

Follicle Stimulating Hormone ◽

Concomitant Increase ◽

Mrna Expression Profile ◽

Intracellular Storage ◽

Stimulating Hormone

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Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: Modulation by calcium

Acta Endocrinologica ◽

10.1530/acta.0.1250280 ◽

1991 ◽

Vol 125 (3) ◽

pp. 280-285 ◽

Cited By ~ 6

Author(s):

J. Alan Talbot ◽

Ann Lambert ◽

Robert Mitchell ◽

Marek Grabinski ◽

David C. Anderson ◽

...

Keyword(s):

Sertoli Cells ◽

Culture Medium ◽

Follicle Stimulating Hormone ◽

Dibutyryl Camp ◽

Immature Rat ◽

Estradiol Secretion ◽

Old Rats ◽

Stimulating Hormone

Abstract We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/1) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091±322 to 1480±84 pmol/l (p<0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360±45 to 1242±133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 μmol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 μmol/l) and A23187 (2 μmol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by >80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 μmol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 μmol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.

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