The galactopoietic effect of bovine growth hormone in goats is associated with increased concentrations of insulin-like growth factor-I in milk and mammary tissue

1991 ◽  
Vol 128 (3) ◽  
pp. 457-463 ◽  
Author(s):  
C. G. Prosser ◽  
C. Royle ◽  
I. R. Fleet ◽  
T. B. Mepham
Keyword(s):  
Growth Factor ◽  
Mammary Tissue ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Lactating Goats ◽  
Significant Difference ◽  
Igf I ◽  
Growth Factor I ◽  
Group 2 ◽  
Group 1

ABSTRACT Lactating goats exhibiting widely divergent responses to short-term (4 days) treatment with bovine GH (bGH) were retrospectively divided into two groups based on the magnitude of this response. There was no difference between groups in terms of the pretreatment milk yield, but by day 4 of treatment milk secretion had increased by 4·99±2·5 (s.e.m.) ml/h (P > 0·05 compared with pretreatment) for group 1 and 22·9±2·4 ml/h (P< 0·001) for group 2. Plasma GH increased in both groups, but concentrations were significantly higher both before and during treatment in group 1 compared with group 2. Plasma concentrations of insulin-like growth factor-I (IGF-I) increased significantly during bGH treatment for both groups and there was no significant difference between the two until day 4 of treatment when levels of IGF-I in group 1 began to decline, whereas those from group 2 were maintained. Concentrations of IGF-I in milk from goats in group 1 were not significantly altered by GH administration, whereas those in goats in group 2 were increased by 40% (P < 0·01 compared with pretreatment). Levels of IGF-I in mammary secretory tissue from four animals from group 1 were not altered by bGH (2·8±0·2 and 2·77 ±0·08 nmol/kg tissue before and after treatment respectively), but were significantly (P < 0·05) increased in four animals from group 2 (2·80±0·2 and 9·9±1·1 nmol/kg tissue). Thus, it appears that the galactopoietic response in goats was associated with significantly lower levels of GH in plasma after 3 days of treatment and, more strikingly, greater amounts of IGF-I in milk and mammary tissue. This latter observation is consistent with the hypothesis that the effects of bGH on the mammary gland itself are mediated by IGF-I and that the availability of IGF-I to mammary tissue is an important component of the overall galactopoietic response to bGH. Journal of Endocrinology (1991) 128, 457–463

scholarly journals Influence of the level of insulin-like growth factor-I and endostatin in blood serum on the lipid profile indicators in patients with acute myocardial infarction and obesity

2019 ◽  
Vol 85 (4) ◽  
pp. 48-54
Author(s):  
D.V. Martovitskyi
Keyword(s):  
Myocardial Infarction ◽  
Growth Factor ◽  
Blood Serum ◽  
Insulin Like Growth Factor ◽  
Elisa Kit ◽  
Factor I ◽  
Igf I ◽  
Growth Factor I ◽  
Group 2 ◽  
Group 1

The levels of insulin-like growth factor I and endostatin in the blood serum and their relationship with lipid profile were investigated in patients with acute myocardial infarction and obesity. The object of the study was 105 patients. All patients were divided into two groups: group 1 consisted of patients with AMI and concomitant obesity (n=60), group 2 consisted of AMI patients without obesity (n=45). The control group consisted of 20 practically healthy people. The average age of patients in group 1 was (67.44±1.34) years old, and in group 2 was (66.85±1.72) years old. The content of IGF-I and endostatin was determined by the enzyme immunoassay. To determine IGF-I, an enzyme-linked immunosorbent assay was used using the Human Insulin like growth factor-I ELISA Kit (MEDIAGNOST, Germany). The endostatin level was determined by the enzyme immunoassay using the Endostatin Elisa Kit (BIOMEDICA, Austria). The biochemical study included the determination of the level of TC and HDL, carried out by the peroxidase method using a set of reagents «Cholesterol Liquicolor» from «Human» (Germany) in blood serum stabilized with heparin. The obtained correlations indicate that an increase in the level of endostatin in the blood serum is significantly associated with an increase in the levels of TC, LDL, TG, CA and a decrease in HDL. Also, reliable data were obtained on the feedback between IGF-I and the level of TC, LDL, TG and CA, as well as a direct relationship between the indicators of IGF-I and HDL. The data obtained indicate that endostatin as a marker of angiogenesis is associated with obesity and dyslipidemia, and also indicate the anti-inflammatory and antioxidant properties of IGF-I under conditions of high autoimmune activity.

Mechanism of secretion of plasma insulin-like growth factor-I into milk of lactating goats

1991 ◽  
Vol 131 (3) ◽  
pp. 459-466 ◽  
Author(s):  
C. G. Prosser ◽  
I. R. Fleet ◽  
A. J. Davis ◽  
R. B. Heap
Keyword(s):  
Growth Factor ◽  
Time Course ◽  
Specific Activity ◽  
Gel Filtration ◽  
Free Form ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Lactating Goats ◽  
Igf I ◽  
Growth Factor I

ABSTRACT 125I-Labelled insulin-like growth factor-I (IGF-I) was infused as the free form directly into the pudic artery supplying one gland of lactating goats (n = 6). The infusion was for 60 min and 0·4±0·09% (s.e.m.) of the infusate was secreted into milk from the infused gland during its first passage through that gland. A large proportion of the 125I-labelled IGF-I escaped into the systematic circulation and was secreted into milk of both glands. A total of 5·2±0·4% of infused radioactivity was recovered in milk from both glands from 0 to 720 min. Radioactivity consisted of trichloroacetic acid (TCA)-precipitable and -soluble counts which were shown by gel filtration to be authentic IGF-I and degraded products of the peptide. The amount and time course of TCA-soluble radioactivity in milk from both glands was similar, suggesting degradation of 125I-labelled IGF-I at extramammary sites. Maximum specific activity for 125I-labelled IGF-I in milk from the infused gland was reached 80–120 min after the start of infusion and was 2·5-fold greater than milk from the non-infused gland. The time course of appearance of 125I-labelled IGF-I in milk suggests that transfer was via the transcellular pathway and this was further supported by comparing the pattern of transfer of [14C]sucrose and [14C]amino acids. When excess unlabelled IGF-I was included in the infusate, specific activity in milk from the infused gland was reduced to that of the non-infused gland, indicating a competitive and saturable mechanism of secretion for 125I-labelled IGF-I. Comparison of uptake and secretion of 125I-labelled IGF-I into milk from the non-infused gland with that of endogenous immunoreactive IGF-I suggests that vectorial transport of IGF-I across the mammary gland may be a significant contributor of IGF-I levels in milk. Journal of Endocrinology (1991) 131, 459–466

Increase in milk secretion and mammary blood flow by intra-arterial infusion of insulin-like growth factor-I into the mammary gland of the goat

1990 ◽  
Vol 126 (3) ◽  
pp. 437-443 ◽  
Author(s):  
C. G. Prosser ◽  
I. R. Fleet ◽  
A. N. Corps ◽  
E. R. Froesch ◽  
R. B. Heap
Keyword(s):  
Blood Flow ◽  
Mammary Gland ◽  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Milk Secretion ◽  
Arterial Infusion ◽  
Factor I ◽  
Lactating Goats ◽  
Igf I ◽  
Growth Factor I

ABSTRACT The close-arterial infusion of free insulin-like growth factor-I (IGF-I; 1·1 nmol/min) for 6 h into the pudic artery supplying one mammary gland of lactating goats caused a 25±6% (mean ± s.e.m., n = 6) increase in the rate of milk secretion of that gland. The increase in the rate of milk secretion in the adjacent non-infused gland (14±4%) was not significantly different from that observed during saline infusion (4±5%). Blood flow to the infused gland was increased from 378±26 ml/min 1 h before to 487±56 ml/min approximately 5 h after the start of the infusion of IGF-I, declining to 420±44 ml/min approximately 2 h after the end of the infusion. The total concentration of IGF-I (free and bound) in milk of the infused gland was significantly higher than that of the non-infused gland. The concentrations of IGF-I in carotid arterial plasma samples increased during IGF-I infusion from a mean value of 32±2 nmol/l before to a maximum of 49±3 nmol/l 5 h after the infusion commenced. Circulating concentrations of total IGF-I declined slowly after the infusion with an estimated half-life of 5 h. Infusion of saline alone did not alter mammary blood flow or the concentration of total IGF-I in milk or plasma. The results indicate that the infusion of free IGF-I into the mammary arterial supply enhances milk secretion and mammary blood flow in intact, conscious goats. The more pronounced effect in the infused compared with the non-infused gland suggests that free IGF-I acts directly on the mammary gland. The response in the non-infused gland was attenuated presumably due to association of IGF-I with plasma binding proteins during recirculation. Journal of Endocrinology (1990) 126, 437–443

scholarly journals PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP FOLIKULOGENESIS MENCIT (Mus musculus)

2020 ◽  
Vol 7 (2) ◽  
pp. 102
Author(s):  
Abdullah Abdullah ◽  
Tjuk Imam Restiadi ◽  
Nunuk Dyah Retno Lastuti ◽  
Tita Damayanti ◽  
Wurlina Wurlina ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mus Musculus ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Igf I ◽  
Growth Factor I ◽  
Difference Test

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbreed mare serum (PMS) in mice (Mus musculus) folliculogenesis. The subject of this research were 20 female mice. The research was arranged by Completely Randomized Design (CRD) with four treatments and five replications. The treatment were K0 = 10 ng/ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, and P3 = 40 ng/ml of IGF-I PMS. Observed variables are number of primary, secondary, tertiary and de Graff follicles. During the treatment the estrus cycle was also observed. The data of follicles number were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS significantly affect (p<0,05) on increasing of the primary and secondary follicles number. The addition of IGF-I PMS 20 ng/ml and 40 ng/ml can increase the primary and secondary follicle significantly (p<0,05).

scholarly journals Insulin-Like Growth Factor-I (IGF-I) from Crossbred Pregnant MareSerum to Increase Follicle Number of Mice (Mus musculus)

2017 ◽  
Vol 3 (6) ◽  
pp. 658
Author(s):  
Abdullah Abdullah ◽  
Tjuk IRestiadi ◽  
Nunuk DR Lastuti ◽  
Tita Damayanti ◽  
Wurlina Wurlina ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mus Musculus ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Igf I ◽  
Growth Factor I ◽  
Difference Test

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbred mare serum (PMS) in mice (Mus musculus) folliculogenesis. The subjects of this research were 20 female mice. The research was arranged by Completely Randomized Design (CRD) with four treatments and five replications. The treatments were C0 = 10 ng/ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, and P3 = 40 ng/ml of IGF-I PMS. Observed variables are the number of primary, secondary, tertiary and de Graff follicles. During the treatment, the estrous cycle was also observed. The data of follicles number were analyzed by Analysis of Variance (ANOVA) and followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS significantly affects (p<0.05) on increasing the primary and secondary follicles number. The addition of IGF-I PMS 20 ng/ml and 40 ng/ml can increase the primary and secondary follicles significantly (p<0.05).  Keywords: IGF-I crossbreed mare serum pregnant; follicle; Mus musculus

Regulation of plasma and tissue levels of insulin-like growth factor-I by nutrition and treatment with growth hormone in sheep

1993 ◽  
Vol 136 (2) ◽  
pp. 217-224 ◽  
Author(s):  
K. M. Hua ◽  
R. Ord ◽  
S. Kirk ◽  
Q. J. Li ◽  
S. C. Hodgkinson ◽  
...  
Keyword(s):  
Growth Factor ◽  
Critical Role ◽  
Biceps Femoris ◽  
Insulin Like Growth Factor ◽  
Gh Treatment ◽  
Factor I ◽  
Significant Difference ◽  
Igf I ◽  
Growth Factor I ◽  
Rna Levels

ABSTRACT Tissue and plasma levels of insulin-like growth factor-I (IGF-I), and relative levels of liver IGF-I RNA, were measured in 6-month-old ewe lambs which were well fed (n = 10) or starved (n = 10) for 5 days. Half of each nutrition group was given daily (09.00 h) injections of human GH (hGH; 0·15 mg/kg body weight per day). Blood was sampled daily from 09.00 to 12.00 h at 15-min intervals through jugular vein catheters and the lambs were slaughtered 24 h after the fifth injection of hGH. Tissue and plasma IGF-I was extracted using an acid-ethanol-cryo-precipitation technique and estimated by radioimmunoassay. Tissue IGF-I was corrected for retained plasma IGF-I using tissue and blood haemaglobin levels. Liver IGF-I RNA levels were monitored by in-situ hybridization. Plasma IGF-I (nmol/l) was higher in both the fed group and the fed group given GH treatment. Tissue IGF-I from kidneys (nmol/kg) was also higher (P < 0·001) in the fed group. There was no significant difference in IGF-I concentrations in the muscle biceps femoris or liver between fed and starved lambs. Although GH treatment did not increase IGF-I levels in tissues significantly, IGF-I RNA levels in liver were increased (P = 0·02) in both fed and starved animals. The relative liver IGF-I RNA levels positively correlated with their corresponding tissue IGF-I levels in the fed group and the fed group given GH treatment. The lack of a significant IGF-I response to GH in tissues may be due to either the time at which tissues were sampled after the GH treatment or the dose of GH administered. However, the higher IGF-I concentrations in plasma and kidney from fed compared with starved animals and the positive correlations between liver IGF-I and IGF-I RNA levels suggest that tissue and plasma IGF-I is regulated by nutrition and GH, with nutrition playing a critical role in the regulation of tissue and plasma IGF-I in normal lambs. Journal of Endocrinology (1993) 136, 217–224

scholarly journals Inability of Overexpressed des(1–3)Human Insulin-Like Growth Factor I (IGF-I) to Inhibit Forced Mammary Gland Involution Is Associated with Decreased Expression of IGF Signaling Molecules*

Endocrinology ◽  
2001 ◽  
Vol 142 (4) ◽  
pp. 1479-1488 ◽  
Author(s):  
Darryl L. Hadsell ◽  
Tatiana Alexeenko ◽  
Yann Klemintidis ◽  
Daniel Torres ◽  
Adrian V. Lee
Keyword(s):  
Mammary Gland ◽  
Growth Factor ◽  
Human Insulin ◽  
Mammary Tissue ◽  
Insulin Like Growth Factor ◽  
Messenger Rnas ◽  
Signal Transducers ◽  
Factor I ◽  
Igf I ◽  
Growth Factor I

Abstract Overexpression of des(1–3) human insulin-like growth factor I (IGF-I) in the mammary glands of transgenic mice (WAP-DES) inhibits apoptosis during natural, but not forced, mammary involution. We hypothesized that this differential response would correlate with the expression of IGF signal transducers. Forced and natural involution were analyzed in nontransgenic and WAP-DES mice beginning on day 16 postpartum. During natural involution, mammary gland wet weight was higher and apoptosis was lower in WAP-DES than in nontransgenic mice. The WAP-DES transgene had no effect on these parameters during forced involution. Mammary tissue concentrations of the transgene protein were 2- to 10-fold higher than those of endogenous IGF-I. Western blot analysis of pooled mammary tissue extracts demonstrated only slightly higher phosphorylation of the IGF signal transducers insulin receptor substrate-1 (IRS-1) and Akt in the WAP-DES than in nontransgenic mice. Dramatic early reductions in phospho-IRS-1, phospho-Akt, IRS-1, IRS-2, and Akt proteins occurred during forced, but not natural, involution. The abundance of the IGF-I receptor and the messenger RNAs for the IGF-I receptors, IRS-1 and -2, were not affected by either genotype or involution. These findings support the conclusions that mammary cells lose their responsiveness to insulin-like signals during forced involution, and that posttranscriptional or posttranslational regulation of IRS-1 and IRS-2 may play a role in this loss.

scholarly journals PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP KETEBALAN ENDOMETRIUM MENCIT (Mus musculus)

2020 ◽  
Vol 7 (2) ◽  
pp. 120
Author(s):  
Dyah Ayu Roro Risna Y ◽  
Sri Mulyati ◽  
Roesno Darsono ◽  
Imam Mustofa ◽  
Arimbi Arimbi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mus Musculus ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Recombinant Mouse ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Igf I ◽  
Growth Factor I

The purpose of the research was to know  the effect of Insulin-Like Growth Factor-I (IGFI) derived from pregnant crossbreed mare serum (PMS) on endometrium thickness of mice (Mus musculus). The subject of this research were 35 female mice. The research was arranged by Completely Randomized Design (CRD) with seven treatment and five replications. The treatment were P0 = 10 ng/ml of physiological NaCl, P11 = 10 ng/ml of IGF-I PMS, P12 = 20 ng/ml of IGF-I PMS, P13 = 40 ng/ml of IGF-I PMS, P21 = 10 ng/ml of IGF-I recombinant mouse, P22 = 20 ng/ml of IGF-I recombinant mouse, and P23 = 40 ng/ml of IGF-I recombinant mouse. Observed variables include histopatological endometrium thickness of mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The data was also be analyzed using General Linear Model Univarieted to see the comparison between IGF-I PMS and recombinant mouse. The result showed that the addition of IGF-I PMS did not significantly affect (p>0,05) on endometrium thickness of mice . It showed that did not significantly difference (p>0,05) between the effect of IGF-I PMS and IGF-I recombinant mouse against the endometrium thickness of mice.

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