PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP KETEBALAN ENDOMETRIUM  MENCIT (Mus musculus)

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGFI) derived from pregnant crossbreed mare serum (PMS) on endometrium thickness of mice (Mus musculus). The subject of this research were 35 female mice. The research was arranged by Completely Randomized Design (CRD) with seven treatment and five replications. The treatment were P0 = 10 ng/ml of physiological NaCl, P11 = 10 ng/ml of IGF-I PMS, P12 = 20 ng/ml of IGF-I PMS, P13 = 40 ng/ml of IGF-I PMS, P21 = 10 ng/ml of IGF-I recombinant mouse, P22 = 20 ng/ml of IGF-I recombinant mouse, and P23 = 40 ng/ml of IGF-I recombinant mouse. Observed variables include histopatological endometrium thickness of mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The data was also be analyzed using General Linear Model Univarieted to see the comparison between IGF-I PMS and recombinant mouse. The result showed that the addition of IGF-I PMS did not significantly affect (p>0,05) on endometrium thickness of mice . It showed that did not significantly difference (p>0,05) between the effect of IGF-I PMS and IGF-I recombinant mouse against the endometrium thickness of mice.

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PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP FOLIKULOGENESIS MENCIT (Mus musculus)

Ovozoa Journal of Animal Reproduction ◽

10.20473/ovz.v7i2.2018.102-108 ◽

2020 ◽

Vol 7 (2) ◽

pp. 102

Author(s):

Abdullah Abdullah ◽

Tjuk Imam Restiadi ◽

Nunuk Dyah Retno Lastuti ◽

Tita Damayanti ◽

Wurlina Wurlina ◽

...

Keyword(s):

Growth Factor ◽

Mus Musculus ◽

Insulin Like Growth Factor ◽

Factor I ◽

Randomized Design ◽

Honestly Significant Difference ◽

Significant Difference ◽

Igf I ◽

Growth Factor I ◽

Difference Test

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbreed mare serum (PMS) in mice (Mus musculus) folliculogenesis. The subject of this research were 20 female mice. The research was arranged by Completely Randomized Design (CRD) with four treatments and five replications. The treatment were K0 = 10 ng/ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, and P3 = 40 ng/ml of IGF-I PMS. Observed variables are number of primary, secondary, tertiary and de Graff follicles. During the treatment the estrus cycle was also observed. The data of follicles number were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS significantly affect (p<0,05) on increasing of the primary and secondary follicles number. The addition of IGF-I PMS 20 ng/ml and 40 ng/ml can increase the primary and secondary follicle significantly (p<0,05).

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Insulin-Like Growth Factor-I (IGF-I) from Crossbred Pregnant MareSerum to Increase Follicle Number of Mice (Mus musculus)

KnE Life Sciences ◽

10.18502/kls.v3i6.1195 ◽

2017 ◽

Vol 3 (6) ◽

pp. 658

Author(s):

Abdullah Abdullah ◽

Tjuk IRestiadi ◽

Nunuk DR Lastuti ◽

Tita Damayanti ◽

Wurlina Wurlina ◽

...

Keyword(s):

Growth Factor ◽

Mus Musculus ◽

Insulin Like Growth Factor ◽

Factor I ◽

Randomized Design ◽

Honestly Significant Difference ◽

Significant Difference ◽

Igf I ◽

Growth Factor I ◽

Difference Test

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbred mare serum (PMS) in mice (Mus musculus) folliculogenesis. The subjects of this research were 20 female mice. The research was arranged by Completely Randomized Design (CRD) with four treatments and five replications. The treatments were C0 = 10 ng/ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, and P3 = 40 ng/ml of IGF-I PMS. Observed variables are the number of primary, secondary, tertiary and de Graff follicles. During the treatment, the estrous cycle was also observed. The data of follicles number were analyzed by Analysis of Variance (ANOVA) and followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS significantly affects (p<0.05) on increasing the primary and secondary follicles number. The addition of IGF-I PMS 20 ng/ml and 40 ng/ml can increase the primary and secondary follicles significantly (p<0.05). Keywords: IGF-I crossbreed mare serum pregnant; follicle; Mus musculus

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The Effect of Insulin-Like Growth Factor-I of Pregnant Crossbred Mare Serum and Insulin-Like Growth Factor-I Recombinant Mouse on Estrous Cycles and Litter Sizes of Mice (Mus musculus)

KnE Life Sciences ◽

10.18502/kls.v3i6.1126 ◽

2017 ◽

Vol 3 (6) ◽

pp. 175

Author(s):

Tjuk Imam Restiadi ◽

Imam Mustofa ◽

Suzanita Utama ◽

Sri Mulyati

Keyword(s):

Growth Factor ◽

Estrous Cycle ◽

Litter Size ◽

Mus Musculus ◽

Insulin Like Growth Factor ◽

Estrous Cycles ◽

Factor I ◽

Recombinant Mouse ◽

Igf I ◽

Growth Factor I

The research was conducted to determine the effect of insulin-like growth factor-I (IGF-I) derived from pregnant crossbred mare serum (PMS) on estrous cycle and litter size in mice (Mus musculus). The research was arranged by Completely Randomized Design (CRD) with seven treatments and five replications. The treatments were control (CO)= physiological NaCl, treatment (T) 1 = 10 ng/ml of IGF-I pregnant mares crossbred serum (PMS), T2 = 20 ng/ml of IGF-I PMS, T3 = 40 ng/ml of IGF-I PMS, T4 = 10 ng/ml of IGF-I recombinant mouse, T5 = 20 ng/ml of IGF-I recombinant mouse, and T6 = 40 ng/ml of IGF-I recombinant mouse. Serie I the subject of this research were 35 female mice. Observed variables included estrous cycle phases that proestrus, estrus, metestrus, and diestrus phase. Serie II for the objects of this research were 35 female mice and 21 male mice in the same treatment on litter size in mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS did not significantly affect (p>0.05) on proestrus, metestrus, and diestrus phase. The addition of IGF-I PMS 40 ng/ml was extended (p<0.05) the estrus phase. It showed that there was not significantly difference (p>0.05) between the effect of IGF-I PMS and IGF-I recombinant mouse with the mice estrous cycles. The result of IGF-I derived from PMS and IGF-I recombinant mouse on litter size in mice did not significantly difference (p>0.05). Test results shows significant differences between control and 40 ng/ml of IGF-I PMS. Keywords: IGF-I, IGF-I pregnant crossbred mare serum, estrous cycle, litter sizes, Mus musculus

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The galactopoietic effect of bovine growth hormone in goats is associated with increased concentrations of insulin-like growth factor-I in milk and mammary tissue

Journal of Endocrinology ◽

10.1677/joe.0.1280457 ◽

1991 ◽

Vol 128 (3) ◽

pp. 457-463 ◽

Cited By ~ 22

Author(s):

C. G. Prosser ◽

C. Royle ◽

I. R. Fleet ◽

T. B. Mepham

Keyword(s):

Growth Factor ◽

Mammary Tissue ◽

Insulin Like Growth Factor ◽

Factor I ◽

Lactating Goats ◽

Significant Difference ◽

Igf I ◽

Growth Factor I ◽

Group 2 ◽

Group 1

ABSTRACT Lactating goats exhibiting widely divergent responses to short-term (4 days) treatment with bovine GH (bGH) were retrospectively divided into two groups based on the magnitude of this response. There was no difference between groups in terms of the pretreatment milk yield, but by day 4 of treatment milk secretion had increased by 4·99±2·5 (s.e.m.) ml/h (P > 0·05 compared with pretreatment) for group 1 and 22·9±2·4 ml/h (P< 0·001) for group 2. Plasma GH increased in both groups, but concentrations were significantly higher both before and during treatment in group 1 compared with group 2. Plasma concentrations of insulin-like growth factor-I (IGF-I) increased significantly during bGH treatment for both groups and there was no significant difference between the two until day 4 of treatment when levels of IGF-I in group 1 began to decline, whereas those from group 2 were maintained. Concentrations of IGF-I in milk from goats in group 1 were not significantly altered by GH administration, whereas those in goats in group 2 were increased by 40% (P < 0·01 compared with pretreatment). Levels of IGF-I in mammary secretory tissue from four animals from group 1 were not altered by bGH (2·8±0·2 and 2·77 ±0·08 nmol/kg tissue before and after treatment respectively), but were significantly (P < 0·05) increased in four animals from group 2 (2·80±0·2 and 9·9±1·1 nmol/kg tissue). Thus, it appears that the galactopoietic response in goats was associated with significantly lower levels of GH in plasma after 3 days of treatment and, more strikingly, greater amounts of IGF-I in milk and mammary tissue. This latter observation is consistent with the hypothesis that the effects of bGH on the mammary gland itself are mediated by IGF-I and that the availability of IGF-I to mammary tissue is an important component of the overall galactopoietic response to bGH. Journal of Endocrinology (1991) 128, 457–463

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Regulation of plasma and tissue levels of insulin-like growth factor-I by nutrition and treatment with growth hormone in sheep

Journal of Endocrinology ◽

10.1677/joe.0.1360217 ◽

1993 ◽

Vol 136 (2) ◽

pp. 217-224 ◽

Cited By ~ 20

Author(s):

K. M. Hua ◽

R. Ord ◽

S. Kirk ◽

Q. J. Li ◽

S. C. Hodgkinson ◽

...

Keyword(s):

Growth Factor ◽

Critical Role ◽

Biceps Femoris ◽

Insulin Like Growth Factor ◽

Gh Treatment ◽

Factor I ◽

Significant Difference ◽

Igf I ◽

Growth Factor I ◽

Rna Levels

ABSTRACT Tissue and plasma levels of insulin-like growth factor-I (IGF-I), and relative levels of liver IGF-I RNA, were measured in 6-month-old ewe lambs which were well fed (n = 10) or starved (n = 10) for 5 days. Half of each nutrition group was given daily (09.00 h) injections of human GH (hGH; 0·15 mg/kg body weight per day). Blood was sampled daily from 09.00 to 12.00 h at 15-min intervals through jugular vein catheters and the lambs were slaughtered 24 h after the fifth injection of hGH. Tissue and plasma IGF-I was extracted using an acid-ethanol-cryo-precipitation technique and estimated by radioimmunoassay. Tissue IGF-I was corrected for retained plasma IGF-I using tissue and blood haemaglobin levels. Liver IGF-I RNA levels were monitored by in-situ hybridization. Plasma IGF-I (nmol/l) was higher in both the fed group and the fed group given GH treatment. Tissue IGF-I from kidneys (nmol/kg) was also higher (P < 0·001) in the fed group. There was no significant difference in IGF-I concentrations in the muscle biceps femoris or liver between fed and starved lambs. Although GH treatment did not increase IGF-I levels in tissues significantly, IGF-I RNA levels in liver were increased (P = 0·02) in both fed and starved animals. The relative liver IGF-I RNA levels positively correlated with their corresponding tissue IGF-I levels in the fed group and the fed group given GH treatment. The lack of a significant IGF-I response to GH in tissues may be due to either the time at which tissues were sampled after the GH treatment or the dose of GH administered. However, the higher IGF-I concentrations in plasma and kidney from fed compared with starved animals and the positive correlations between liver IGF-I and IGF-I RNA levels suggest that tissue and plasma IGF-I is regulated by nutrition and GH, with nutrition playing a critical role in the regulation of tissue and plasma IGF-I in normal lambs. Journal of Endocrinology (1993) 136, 217–224

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Pengaruh Pemberian Hormon Igf-I (Insulin-Like Growth Factor-I) Recombinant Mouse terhadap Pertumbuhan Ikan Gurame (Osphronemus Gouramy) [ Effect of hormones Recombinant Mouse IGF-I (Insulin-Like Growthfactor-I) on The Growth of Osphronemus gouramy]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v8i1.11190 ◽

2019 ◽

Vol 8 (1) ◽

pp. 45

Author(s):

Dimas Eraparamarta Harkitiyanto ◽

Woro Hastuti Satyantini ◽

Prayogo Prayogo

Keyword(s):

Growth Factor ◽

Cell Metabolism ◽

Scientific Information ◽

Fish Feed ◽

Insulin Like Growth Factor ◽

Factor I ◽

Recombinant Mouse ◽

Hormone Injection ◽

Igf I ◽

Growth Factor I

Abstrak Konsumsi ikan gurame semakin meningkat setiap tahun mengakibatkan tingginya jumlah permintaan, belum dapat diimbangi produksi yang mencukupi. Laju pertumbuhan ikan gurame dikenal lambat, sehingga untuk mencapai ukuran konsumsi diperlukan waktu pemeliharaan relatif lama. Peningkatan kualitas produksi dengan meningkatkan kemampuan metabolisme sel terhadap asupan pakan ikan melalui penyuntikan hormon insulin-like growth factor-I(IGF-I) diharapkan dapat memacu pertumbuhan, dan produksi ikan dapat ditingkatkan. Tujuan dari penelitian ini adalahuntuk mengetahui pengaruh pemberian hormon (IGF-I) recombinant mouse terhadap peningkatan pertumbuhan ikan gurame(Osphronemus gouramy). Penelitian adalah metode eksperimental rancangan acak lengkap (RAL), menggunakan ikan gurame sebanyak 80 ekor yang terbagi dalam 4 perlakuanP0 : tanpa disuntik hormon IGF-I, P1 : disuntik hormon IGF-I dengan dosis 10 ng/ml, P2 : disuntik hormon dengan dosis 20 ng/ml dan P3 : disuntik hormon dengan dosis 40 ng/ml masing-masing 5 ulangan setiap ulangan 4 ekor ikan gurame. Perlakuan dengan injeksi hormon IGF-I recombinant mouse. Parameter yang diukur adalah pertumbuhan. Hasil penelitian menunjukkan bahwa perlakuan penyuntikan hormon IGF-I recombinant mouse dengan dosis yang berbeda memberikan perbedaan yang nyata (p<0,05); pertumbuhan berat (P0: 0 ng/ml = 7,23 gram± 0,37 gram dan P3: 40 ng/ml = 26,35 gram± 1,89 gram) ;pertumbuhan panjang (P0 : 0 ng/ml = 5,76 cm ± 0,38 cm dan P3 : 40 ng/ml = 7,93 cm± 0,07 cm).). Kesimpulan penelitian bahwa IGF-I recombinant mouse berpengaruh meningkatkan pertumbuhan pada peningkatan berat dan panjang ikan gurame pada perlakuan penyuntikan 40 ng/ml. Abstract Osphronemus gouramy consumption is increasing every year which causes in a high number of inquiries for this fish, cannot be offset sufficiently by the amount of production. The growth rate of O. gouramy are known relatively slow, so that to achieve the required consumption is relatively long. Quality improvement of productivity by improving the ability of cell metabolism to intake of fish feed through hormone injection recombinant mouse insulin-like growth factor-I (IGF-I) is expected to spur growth, so that fish production can be increased. The aim of this study is to determine the effect of the hormone IGF-I recombinant mouse to the increased growth of O.gouramy. The benefits of this research are expected to deliver the benefitsof science in the form of scientific information to the public in general and fish farmers especially about the effect of the hormone recombinant mouse IGF-I on the growth of O. gouramy.The research is methodis carried out experimentally by using completely randomized design (CRD),this study uses 80 O. gouramy were divided into 4 treatment P0: without hormone injectionsof IGF-I, P1: injected with the hormone IGF-I at a dose of 10 ng/ml, P2: injected with the hormone IGF-I at a dose of 20 ng/ml and P3: injected with the hormone ata dose of 40ng/ml each of 5 replicates each test 4 O. gouramy. The hormone IGF-I recombinant mouse is done by injection. The treatment with hormone injection of recombinant mouse IGF-I. Parameters measured were growth. The results showed that the treatment hormone injection of recombinant mouse IGF-I with different doses gave significant differences (p <0.05);weight growth (P0: 0 ng/ml = 7,23 gram ± 0,37 gram dan P3: 40 ng/ml = 26,35 gram ± 1,89 gram.); length growth (P0 : 0 ng/ml = 5,76 cm ± 0,38 cm dan P3 : 40 ng/ml = 7,93 cm ± 0,07 cm). The conclusion that recombinant mouse IGF-Iincreased the growth by increasing in weight and length of O. gouramy and improvement of the growth occurred in the treatment injection of the dose: 40ng/ml.

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