scholarly journals Scanning Electron Microscopy of the Small Intestine during gluten-Challenge in Celiac Disease.

1992 ◽  
Vol 55 (Suppl) ◽  
pp. 125-130 ◽  
Author(s):  
F. M. MAGLIOCCA ◽  
M. BONAMICO ◽  
V. PETROZZA ◽  
S. CORRER ◽  
M. MONTUORI ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Small Intestine ◽  
Celiac Disease ◽  
Scanning Electron

scholarly journals Infection of the Colon of the Rhesus Monkey by Spiral-Shaped Organisms

1982 ◽  
Vol 19 (7_suppl) ◽  
pp. 26-32 ◽  
Author(s):  
J. Zeller ◽  
A. Takeuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Small Intestine ◽  
Macaca Mulatta ◽  
Brush Border ◽  
Rhesus Monkeys ◽  
Clinically Normal ◽  
Intestinal Spirochetosis ◽  
Hematoxylin And Eosin ◽  
Scanning Electron

Intestinal spirochetosis, an infection of the mucosa by spiral-shaped organisms, was studied in clinically normal rhesus monkeys (Macaca mulatta) by histology, transmission and scanning electron microscopy. The incidence of intestinal spirochetosis was 42% in 221 monkeys. Spiral organisms stained with hematoxylin and eosin (HE) appeared as a broad basophilic haze on the colonic surface and were strongly positive by the Warthin-Starry stain. Spiral-shaped bacteria include two structurally different organisms: spirochetes and flagellated microbes. They intimately populated the brush border of the surface of the epithelium of the large intestine. They were absent in the crypts and in the small intestine. Infection by spirochetes produced no alteration of cytocomponents of the underlying host structures. Spirochetes and flagellates infrequently penetrated beyond the brush border into the epithelial cytoplasm and also into the lamina propria. Even in cases where invasion was documented, no inflammatory response was found.

scholarly journals Rapid chemical dehydration of biologic samples for scanning electron microscopy using 2,2-dimethoxypropane.

1977 ◽  
Vol 25 (4) ◽  
pp. 247-251 ◽  
Author(s):  
M D Maser ◽  
J J Trimble
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Small Intestine ◽  
Cultured Fibroblasts ◽  
Critical Point Drying ◽  
Mouse Small Intestine ◽  
Fine Surface ◽  
Plastic Embedding ◽  
Rat Trachea ◽  
Scanning Electron

Acidified 2,2-dimethoxypropane (DMP) has been used as a rapid chemical dehydrating agent en route to plastic embedding and ulthrathin sectioning for conventional electron miscroscopy (J Histochem Cytochem 23:107, 1975). We have used DMP to dehydrate biologic specimens prior to critical point drying and metal coating for scanning electron microscopy. There is no difference in either the gross architecture or the fine surface structure of mouse small intestine and trachea, rat trachea and kidney, and cultured fibroblasts, between samples dehydrated in DMP for 5 min to 30 days and those conventionally dehydrated in ethanol or acetone. DMP dehydration is advantageous in speed, economy and apparent completeness.

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