scholarly journals Infection of the Colon of the Rhesus Monkey by Spiral-Shaped Organisms

1982 ◽  
Vol 19 (7_suppl) ◽  
pp. 26-32 ◽  
Author(s):  
J. Zeller ◽  
A. Takeuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Small Intestine ◽  
Macaca Mulatta ◽  
Brush Border ◽  
Rhesus Monkeys ◽  
Clinically Normal ◽  
Intestinal Spirochetosis ◽  
Hematoxylin And Eosin ◽  
Scanning Electron

Intestinal spirochetosis, an infection of the mucosa by spiral-shaped organisms, was studied in clinically normal rhesus monkeys (Macaca mulatta) by histology, transmission and scanning electron microscopy. The incidence of intestinal spirochetosis was 42% in 221 monkeys. Spiral organisms stained with hematoxylin and eosin (HE) appeared as a broad basophilic haze on the colonic surface and were strongly positive by the Warthin-Starry stain. Spiral-shaped bacteria include two structurally different organisms: spirochetes and flagellated microbes. They intimately populated the brush border of the surface of the epithelium of the large intestine. They were absent in the crypts and in the small intestine. Infection by spirochetes produced no alteration of cytocomponents of the underlying host structures. Spirochetes and flagellates infrequently penetrated beyond the brush border into the epithelial cytoplasm and also into the lamina propria. Even in cases where invasion was documented, no inflammatory response was found.

scholarly journals White Scours in Piglets; II. Scanning Electron Microscopy of the Mucosa of the Small Intestine

1971 ◽  
Vol 8 (5-6) ◽  
pp. 401-413 ◽  
Author(s):  
J. M. V. M. Mouwen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Small Intestine ◽  
Small Intestinal Mucosa ◽  
Clinically Normal ◽  
New Findings ◽  
Villous Surface ◽  
Small Intestinal ◽  
Scanning Electron ◽  
High Range

The mucosa of the small intestine of clinically normal piglets and of piglets with white scours was investigated, using a scanning electron microscope. No new findings on the rough villous pattern of the small intestinal mucosa could be added to those obtained with the stereomicroscope. In the high range of magnification, however, the surface of the different villous forms showed features that could barely be seen with the stereomicroscope. The findings indicate that in general the microstructure of the villous surface differs in the various types of villi.

Scanning Electron Microscopy of Human Jejunum in Whipple's Disease

1977 ◽  
Vol 35 ◽  
pp. 354-355
Author(s):  
John H. L. Watson ◽  
C. N. Sun
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Basement Membrane ◽  
Lamina Propria ◽  
Brush Border ◽  
Whipple’S Disease ◽  
Whipple's Disease ◽  
Biopsy Specimens ◽  
Brush Borders ◽  
Scanning Electron

That the etiology of Whipple's disease could be bacterial was first suggested from electron micrographs in 1960. Evidence for binary fission of the bacteria, their phagocytosis by histiocytes in the lamina propria, their occurrence between and within the cells of the epithelium and on the brush border of the lumen were reported later. Scanning electron microscopy has been applied by us in an attempt to confirm the earlier observations by the new technique and to describe the bacterium further. Both transmission and scanning electron microscopy have been used concurrently to study the same biopsy specimens, and transmission observations have been used to confirm those made by scanning.The locations of the brush borders, the columnar epithelial cells, the basement membrane and the lamina propria beneath it were each easily identified by scanning electron microscopy. The lamina propria was completely filled with the wiener-shaped bacteria, Fig. 1.

scholarly journals Scanning Electron Microscopy of the Small Intestine during gluten-Challenge in Celiac Disease.

1992 ◽  
Vol 55 (Suppl) ◽  
pp. 125-130 ◽  
Author(s):  
F. M. MAGLIOCCA ◽  
M. BONAMICO ◽  
V. PETROZZA ◽  
S. CORRER ◽  
M. MONTUORI ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Small Intestine ◽  
Celiac Disease ◽  
Scanning Electron

scholarly journals Rapid chemical dehydration of biologic samples for scanning electron microscopy using 2,2-dimethoxypropane.

1977 ◽  
Vol 25 (4) ◽  
pp. 247-251 ◽  
Author(s):  
M D Maser ◽  
J J Trimble
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Small Intestine ◽  
Cultured Fibroblasts ◽  
Critical Point Drying ◽  
Mouse Small Intestine ◽  
Fine Surface ◽  
Plastic Embedding ◽  
Rat Trachea ◽  
Scanning Electron

Acidified 2,2-dimethoxypropane (DMP) has been used as a rapid chemical dehydrating agent en route to plastic embedding and ulthrathin sectioning for conventional electron miscroscopy (J Histochem Cytochem 23:107, 1975). We have used DMP to dehydrate biologic specimens prior to critical point drying and metal coating for scanning electron microscopy. There is no difference in either the gross architecture or the fine surface structure of mouse small intestine and trachea, rat trachea and kidney, and cultured fibroblasts, between samples dehydrated in DMP for 5 min to 30 days and those conventionally dehydrated in ethanol or acetone. DMP dehydration is advantageous in speed, economy and apparent completeness.

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