scholarly journals Prevalence and genetic diversity of cucumber mosaic virus isolates infecting tomato plants in Marmara region of Turkey

2021 ◽  
Author(s):  
Ali KARANFİL
Keyword(s):  
Genetic Diversity ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Marmara Region ◽  
Tomato Plants ◽  
Virus Isolates

scholarly journals Cucumber mosaic virus Isolates from Greek Legumes are Associated with Satellite RNAs that are Necrogenic for Tomato

Plant Disease ◽  
2018 ◽  
Vol 102 (11) ◽  
pp. 2268-2276 ◽  
Author(s):  
Antonis Giakountis ◽  
Iason Tsarmpopoulos ◽  
Elisavet K. Chatzivassiliou
Keyword(s):  
Genetic Diversity ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Protein Sequence ◽  
Coat Protein Sequence ◽  
Tomato Plants ◽  
Satellite Rnas ◽  
Virus Isolates ◽  
Limited Genetic Diversity

Worldwide, Cucumber mosaic virus (CMV) is the causal agent of many economically important diseases. Based on immunological or molecular analysis, three distinct subgroups of CMV isolates can be identified (IA, IB, and II). In addition, some CMV isolates are associated with satellite RNAs (satRNAs), a type of noncoding transcript that may alter the symptoms of CMV infections. This study presents an analysis of CMV isolates occurring in legumes in Greece in respect to their genetic diversity, and the presence and diversity of their satRNA. Phylogenetic analysis of the CMV coat protein sequence of 18 legume and 5 tomato CMV isolates collected throughout Greece classified them within subgroups IA and IB, with a limited genetic diversity. The CMV satRNAs found in nine field legumes exhibiting mild symptoms and in one tomato with a necrotic syndrome contained a functional necrogenic motif; therefore, they were grouped within the necrogenic group of CMV-satRNAs. The necrotic phenotype was expressed in all legume CMV isolates containing necrogenic satRNAs when mechanically inoculated onto tomato plants. To our knowledge, this is the first observation that legumes host necrogenic CMV-satRNAs. The possible role of legumes in the epidemiology of CMV and necrogenic satRNA complex is discussed.

Genetic diversity, distant phylogenetic relationships and the occurrence of recombination events among cucumber mosaic virus isolates from zucchini in Poland

2017 ◽  
Vol 162 (6) ◽  
pp. 1751-1756 ◽  
Author(s):  
Beata Hasiów-Jaroszewska ◽  
Mateusz Chrzanowski ◽  
Daria Budzyńska ◽  
Natalia Rymelska ◽  
Natasza Borodynko-Filas
Keyword(s):  
Genetic Diversity ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Phylogenetic Relationships ◽  
Virus Isolates

scholarly journals Genetic Structure and Molecular Variability of Cucumber mosaic virus Isolates in the United States

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96582 ◽  
Author(s):  
Shahideh Nouri ◽  
Rafael Arevalo ◽  
Bryce W. Falk ◽  
Russell L. Groves
Keyword(s):  
United States ◽  
Genetic Structure ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
The United States ◽  
Molecular Variability ◽  
Virus Isolates

scholarly journals Analysis of Mechanisms Involved in the Cucumber mosaic virus Satellite RNA-mediated Transgenic Resistance in Tomato Plants

2004 ◽  
Vol 17 (1) ◽  
pp. 98-108 ◽  
Author(s):  
Fabrizio Cillo ◽  
Mariella M. Finetti-Sialer ◽  
Maria A. Papanice ◽  
Donato Gallitelli
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Resistance Mechanism ◽  
Degradation Process ◽  
Transgenic Tomato ◽  
Satellite Rna ◽  
Total Recovery ◽  
Tomato Plants ◽  
Free Strain

Transgenic tomato (Lycopersicon esculentum Mill. cv. UC82) plants expressing a benign variant of Cucumber mosaic virus satellite RNA (CMV Tfn-satRNA) were generated. The transformed plants did not produce symptoms when challenged with a satRNA-free strain of CMV (CMV-FL). The same plant lines initially were susceptible to necrosis elicited by a CMV strain supporting a necrogenic variant of satRNA (CMV-77), but a phenotype of total recovery from the necrosis was observed in the newly developing leaves. The features of the observed resistance were analyzed and are consistent with two different mechanisms of resistance. In transgenic plants inoculated with CMV-FL strain, the symptomless phenotype was correlated to the down-regulation of CMV by Tfn-satRNA, amplified from the transgene transcripts, as the first resistance mechanism. On the other hand, the delayed resistance to CMV-77 in transgenic tomato lines was mediated by a degradation process that targets satRNAs in a sequence-specific manner. Evidence is provided for a correlation between a reduced accumulation level of transgenic messenger Tfn-satRNA, the accumulation of small (approximately 23 nucleotides) RNAs with sequence homology to satRNAs, the progressively reduced accumulation of 77-satRNA in infected tissues, and the transition in infected plants from diseased to healthy. Thus, events leading to the degradation of satRNA sequences indicate a role for RNA silencing as the second mechanism determining resistance of transgenic tomato lines.

Comparative transmission of two cucumber mosaic virus isolates by two color morphs of Acyrthosiphon pisum (Harris)

2012 ◽  
Vol 56 (02) ◽  
pp. 139-143 ◽  
Author(s):  
A. Tahmasebi ◽  
A. Dizadji ◽  
F. Farhoudi ◽  
H. Allahyari ◽  
M. Koohi-Habibi
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Acyrthosiphon Pisum ◽  
Color Morphs ◽  
Virus Isolates

scholarly journals Occurrence of Cucumber mosaic virus Subgroups IA and IB Isolates in Tomatoes in Nigeria

Plant Disease ◽  
2014 ◽  
Vol 98 (12) ◽  
pp. 1750-1750 ◽  
Author(s):  
A. B. Kayode ◽  
B. O. Odu ◽  
K. A. Ako-Nai ◽  
O. J. Alabi
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Bootstrap Support ◽  
Acid Extraction ◽  
International Institute ◽  
Weed Species ◽  
Southwestern Nigeria ◽  
Tomato Plants ◽  
Southwest Nigeria ◽  
Specific Sequences

Tomato (Solanum lycopersicum L.) is a major economic crop consumed globally in fresh or processed forms. During a routine field survey of major tomato-producing areas of southwestern Nigeria in May/June 2013, tomato plants cv. Roma VF showing virus-like symptoms including stunting, chlorosis, and narrowing of leaf blades were observed in 10 farmers' fields with varying levels of incidence averaging ~27%. Moderate to high aphid infestations were also observed in affected fields, and fruit production was significantly impacted based on visual observations. Since symptoms observed on affected plants are similar to those described for Cucumber mosaic virus (CMV) infection in tomato (5), leaf tissue samples collected from a total of 92 tomato plants across 10 commercial farms were subjected to antigen coated plate (ACP)-ELISA essentially as described previously (2). In ACP-ELISA using a CMV polyclonal antibody, 24 of the 92 samples (26.1%) derived from 7 of the 10 survey locations spread across Oyo, Ogun, Ekiti, and Osun states of southwestern Nigeria tested positive for CMV. Based on the ACP-ELISA results, one randomly selected sample from each of the CMV-positive survey locations, seven samples in total, was subjected to total nucleic acid extraction (1) followed by one step-single tube RT-PCR using primers CMV1/CMV2 and conditions described previously (4) with appropriate virus-positive and -negative controls. A ~500 bp DNA band was amplified from these seven ACP-ELISA-positive samples, thus confirming the presence of CMV. To further confirm these results and to enable molecular typing of CMV isolates from southwest Nigeria, the amplified DNA fragments were precipitated with the addition of 70% ethanol and centrifugation and directly sequenced using the ABI 3130xL Genetic Analyzer (Applied Biosystems, California) at the Bioscience Center of the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria. Following the removal of primer- and 3′UTR-specific sequences, the remaining 366-bp partial CP-specific sequences (GenBank Accession Nos. KM091952 to 58) and corresponding sequences of global CMV isolates obtained from GenBank were subjected to multiple alignments using the MEGA 6.0 software. This analysis showed that tomato-infecting CMV isolates from southwest Nigeria shared 91.6 to 99.4% and 94.9 to 99.1% nucleotide (nt) and amino acid (aa) identities among themselves and 91.6 to 98.0% and 94.1 to 98.3%, 89.4 to 94.1% and 93.2 to 98.3%, and 75.2 to 78.8% and 84.0 to 87.3% with corresponding nt and aa sequences of representatives of CMV isolates belonging to subgroups IA (D10538), IB (AB008777), and II (M21464), respectively. Maximum likelihood phylogenetic analysis revealed the clustering of four and three CMV isolates obtained in this study into subgroups IA and IB, respectively, with >70% bootstrap support. CMV has been detected in tomato seeds (3) and its very wide host range includes cultivated crops and weed species (5). It is therefore plausible that contaminated seed lots and alternative weed and crop host plants serve as sources of CMV inoculum to cultivated tomato in affected farms. Although CMV has been reported from tomato from several countries worldwide, to our knowledge, this is the first empirical evidence for the occurrence of CMV subgroups IA and IB in cultivated tomato in Nigeria. References: (1) S. L. Dellaporta et al. Plant Mol. Biol. Rep. 1:19, 1983. (2) J. d'A. Hughes and S. A. Tarawali. Trop. Sci. 39:70, 1999. (3) K. H. Park and B. J. Cha. Res. Plant Dis. 8:101, 2002. (4) S. Wylie et al. Aus. J. Agric. Res. 44:41, 1993. (5) T. A. Zitter and J. F. Murphy. Plant Health Instructor. DOI: 10.1094/PHI-I-2009-0518-01, 2009.

scholarly journals Emergence and Characterization of Satellite RNAs Associated with Spanish Cucumber Mosaic Virus Isolates

1984 ◽  
Vol 65 (3) ◽  
pp. 539-547 ◽  
Author(s):  
I. Garcia-Luque ◽  
J. M. Kaper ◽  
J. R. Diaz-Ruiz ◽  
M. Rubio-Huertos
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Satellite Rnas ◽  
Virus Isolates
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