scholarly journals Detection of Campylobacter spp. from Poultry and Poultry Products in Dhaka, Bangladesh

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Sultana S
Keyword(s):  
Poultry Products ◽  
Campylobacter Spp

Occurrence of Pathogens in Raw and Ready-to-Eat Meat and Poultry Products Collected from the Retail Marketplace in Edmonton, Alberta, Canada

2006 ◽  
Vol 69 (9) ◽  
pp. 2176-2182 ◽  
Author(s):  
V. M. BOHAYCHUK ◽  
G. E. GENSLER ◽  
R. K. KING ◽  
K. I. MANNINEN ◽  
O. SORENSEN ◽  
...  
Keyword(s):  
Shiga Toxin ◽  
Ground Beef ◽  
Fermented Sausage ◽  
E Coli ◽  
Poultry Products ◽  
Fermented Sausages ◽  
Roast Beef ◽  
Chicken Legs ◽  
Retail Products ◽  
Campylobacter Spp

A total of 800 meat and poultry products were purchased from the retail marketplace in Edmonton, Alberta, Canada. The products consisted of raw ground beef, chicken legs, pork chops, and ready-to-eat fermented sausage, roast beef, processed turkey breast, chicken wieners, and beef wieners. The samples were analyzed to determine the prevalence of Shiga toxin–producing Escherichia coli, Salmonella, Campylobacter spp., and Listeria monocytogenes. Shiga toxin–producing E. coli O22: H8 was found in one raw ground beef sample. Salmonella and Campylobacter were found in 30 and 62% of raw chicken legs, respectively. L. monocytogenes was found in 52% of raw ground beef, 34% of raw chicken legs, 24% of raw pork chops, 4% of fermented sausages, 3% of processed turkey breast, 5% of beef wieners, and 3% of chicken wieners. The occurrence of pathogens in this study is similar to that in retail products in many other international locales.

Method-Dependent Variability in Determination of Prevalence of Campylobacter jejuni and Campylobacter coli in Canadian Retail Poultry

2014 ◽  
Vol 77 (10) ◽  
pp. 1682-1688 ◽  
Author(s):  
CATHERINE D. CARRILLO ◽  
DANIEL PLANTE ◽  
IRÈNE IUGOVAZ ◽  
ROBYN KENWELL ◽  
GHISLAINE BÉLANGER ◽  
...  
Keyword(s):  
Campylobacter Coli ◽  
Testing Methods ◽  
Poultry Products ◽  
Thermophilic Campylobacter ◽  
Microbiological Methods ◽  
Bacterial Gastroenteritis ◽  
Campylobacter Species ◽  
Raw Poultry ◽  
Campylobacter Spp

Campylobacter is the most frequent cause of bacterial gastroenteritis in Canada, and the illness is commonly associated with poultry consumption. Whereas Canadian retail poultry is often contaminated with campylobacters, studies on the prevalence of this organism are inconsistent due to variability in sampling and microbiological methodology. To determine the current microbiological status of Canadian poultry, and to evaluate two commonly used microbiological methods, 348 raw poultry samples were collected at retail across Canada over a period of 3 years (2007 to 2010) and were analyzed for the presence of thermophilic Campylobacter species. The overall prevalence of Campylobacter spp. was found to be 42.8% by a combination of the two testing methods, with 33.9% of the samples positive for C. jejuni, 3.7% of the samples positive for C. coli, and 5.2% of the samples positive for both. Variability in Campylobacter spp. prevalence was observed in samples obtained from different regions across Canada and from poultry with or without skin, but this was not statistically significant. In co-contaminated samples, C. jejuni was preferentially recovered from Preston agar compared with mCCDA and Campy-Cefex agar, with an increase in recovery of C. coli on all selective media after 48 h of enrichment. A subset of 214 of the poultry rinses were analyzed by both Health Canada's standard method, MFLP-46 (enrichment in Park and Sanders broth), and a second method requiring enrichment in Bolton broth. Significantly more positive samples were obtained with the MFLP-46 method (40.6%) than with the alternate method (35.0%). This improved recovery with MFLP-46 may be due to the omission of cycloheximide from this method. These results demonstrate that determination of prevalence of Campylobacter spp. on poultry products may be significantly impacted by the choice of microbiological methods used. Canadian poultry continues to be a source of exposure to Campylobacter spp.

scholarly journals Arsenic Resistance in Campylobacter spp. Isolated from Retail Poultry Products

2006 ◽  
Vol 72 (4) ◽  
pp. 3069-3071 ◽  
Author(s):  
Amy R. Sapkota ◽  
Lance B. Price ◽  
Ellen K. Silbergeld ◽  
Kellogg J. Schwab
Keyword(s):  
Growth Promotion ◽  
Poultry Production ◽  
Arsenic Resistance ◽  
Poultry Products ◽  
Campylobacter Spp

ABSTRACT Organoarsenicals are commonly used for growth promotion in U.S. poultry production. Susceptibilities to arsenite, arsenate, and the organoarsenical roxarsone were measured in 251 Campylobacter isolates from conventional and antimicrobial-free retail poultry products. Isolates from conventional poultry products had significantly higher roxarsone MICs (z = 8.22; P < 0.0001).

Prevalence of Campylobacter spp. in poultry and poultry products for sale on the Bulgarian retail market

2007 ◽  
Vol 92 (3) ◽  
pp. 285-288 ◽  
Author(s):  
Todor Stoyanchev ◽  
Ivan Vashin ◽  
Christian Ring ◽  
Viktoria Atanassova
Keyword(s):  
Retail Market ◽  
Poultry Products ◽  
Campylobacter Spp

scholarly journals Prevalence and antibiotic resistance of Campylobacter coli isolated from broiler farms in the Marrakesh Safi region, Morocco

2020 ◽  
Vol 13 (9) ◽  
pp. 1892-1897
Author(s):  
Raja Asmai ◽  
Bouchra Karraouan ◽  
Khadija Es-Soucratti ◽  
Houda En-Nassiri ◽  
Brahim Bouchrif ◽  
...  
Keyword(s):  
Poultry Meat ◽  
Diffusion Method ◽  
Campylobacter Coli ◽  
Disk Diffusion Method ◽  
Poultry Products ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Control Procedures ◽  
Susceptibility Profiles ◽  
Campylobacter Spp

Background and Aim: Campylobacteriosis is a common foodborne disease epidemiologically linked to the consumption of poultry products. However, other sources, such as raw or contaminated milk, contaminated water or ice, contact with infected livestock, and pets, are reported. This study aimed to evaluate the prevalence and resistance to microbial resistance of Campylobacter coliM in broiler farms in the region of Marrakesh Safi, Morocco. Materials and Methods: The study was conducted between May and December 2017 and involved 35 broiler farms. One hundred and five cloacal swabs were collected from the eight provinces in the region of Marrakesh Safi, Morocco. Bacteriology method NM ISO/TS 10272-3: 2013 was used to isolate and identify Campylobacter spp. Molecular identification (polymerase chain reaction) was used for confirmation. A disk diffusion method on Mueller-Hinton agar was used for susceptibility testing. Five antibiotic agents, including first-line drugs, were evaluated. Results: Among 105 samples, 71.4% (75/105) were positive for Campylobacter spp. test and 56% (42/75) of isolates belonged to the species coli. Susceptibility profiles showed that 95.2% of C. coli strains were resistant to ampicillin, 92.8% to erythromycin and tetracycline, 85.7% to ciprofloxacin, and 7.1% to gentamicin. Conclusion: This study underlines the need to strengthen implementation of specific control procedures to decrease contamination of poultry meat with Campylobacter spp. and to reduce the use of antibiotics in the poultry sector.

Assessment of Three Nucleic Acid Hybridization Systems for Detection of Campylobacter spp. in Poultry Products

1994 ◽  
Vol 57 (8) ◽  
pp. 703-709 ◽  
Author(s):  
GERALDINE M. RANSOM ◽  
DAVID W. DREESEN ◽  
BONNIE E. ROSE ◽  
CHARLES P. LATTUADA
Keyword(s):  
Nucleic Acid ◽  
The United States ◽  
Nucleic Acid Hybridization ◽  
United States Department ◽  
Department Of Agriculture ◽  
Poultry Products ◽  
Cultural Method ◽  
Confirmation Test ◽  
Inspection Service ◽  
Campylobacter Spp

Three commercially available nucleic acid hybridization systems were evaluated in combination with the United States Department of Agriculture-Food Safety and Inspection Service (USDA/FSIS) cultural protocol for the detection of Campylobacter spp. from a variety of poultry products. Samples were enriched for 24 h in Hunt broth and then plated onto modified charcoal Campylobacter differential agar. Suspensions of growth from the selective agar plates were then analyzed by the probe assays. The GENE-TRAK® Campylobacter Assay (revised format) and the GEN-PROBE® ACCUPROBE™ Campylobacter Culture Confirmation Test showed sensitivities and specificities of 100% upon testing of 30 raw chicken rinses. The original format GENE-TRAK® test had a sensitivity of 93% and a specificity of 100% when these samples were tested. Ninety percent of the raw chicken rinses were found to contain Campylobacter spp. By either of the two more sensitive probes or by the USDA/FSIS cultural method. Eighty-three percent of the rinses registered Campylobacter-positive by the original format GENE-TRAK® probe. When inoculated ready-to-eat poultry samples were examined, the revised format GENE- TRAK® test and the ACCUPROBE™ assay had sensitivities of 83% and specificities of 100%. The original format GENE-TRAK® test showed a 75% sensitivity and a 100% specificity with these samples. The USDA/FSIS cultural method had a sensitivity of 79% and a specificity of 100% with the inoculated samples. The detection limit of the revised format GENE-TRAK® and the ACCUPROBE™ assays upon testing pooled cell suspensions of four Campylobacter jejuni poultry isolates was approximately 106 CPU/ml.

Prevalence of Campylobacter spp. in Raw Retail Poultry on Sale in Northern Ireland

2009 ◽  
Vol 72 (9) ◽  
pp. 1830-1835 ◽  
Author(s):  
LYNN MORAN ◽  
PAM SCATES ◽  
ROBERT H. MADDEN
Keyword(s):  
United Kingdom ◽  
Northern Ireland ◽  
Incubation Temperature ◽  
Mean Value ◽  
Campylobacter Coli ◽  
Poultry Products ◽  
Campylobacter Lari ◽  
Retail Products ◽  
Human Campylobacteriosis ◽  
Campylobacter Spp

A year-long survey of fresh, retail poultry products on sale in Northern Ireland was undertaken to define the prevalence of Campylobacter spp. by using protocols based on ISO (standard) 10272-1:2006. Incubation at 37 and 42°C was undertaken to increase the diversity of isolates obtained. Overall, 652 isolates were identified as Campylobacter spp. by using PCR and amplified fragment length polymorphic typing. Phenotyping wrongly identified 21% of isolates. Prevalences of Campylobacter found were chicken, 91% (n = 336); turkey, 56% (n = 77); and duck, 100% (n = 17). Prevalence rates for chicken produced in Northern Ireland, Scotland, England, and Wales were similar, with a mean value of 91%. The prevalences in product from the latter two countries were much higher than were found in two United Kingdom–wide surveys of chicken. The incubation temperature did not affect the relative proportions of the species isolated (P &gt; 0.05). Campylobacter jejuni composed 64.6% of isolates, Campylobacter coli, 27.4%, and Campylobacter lari, 1%. Most cases of human campylobacteriosis are caused by C. jejuni and C. coli. The overall Campylobacter prevalence results are consistent with Northern Ireland surveys undertaken since 2000, and indicate that United Kingdom strategies to control Campylobacter in chicken have not had a significant effect on the prevalence of this pathogen in retail products on sale in Northern Ireland.

scholarly journals Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp.

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Sylvain Daton Kougblénou ◽  
Alidéhou Jerrold Agbankpé ◽  
Justin Gbèssohélé Béhanzin ◽  
Tamègnon Victorien Dougnon ◽  
Alidah Victonie Aniambossou ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Irrigation Water ◽  
Poultry Manure ◽  
Food Poisoning ◽  
Leafy Vegetables ◽  
Contamination Rate ◽  
Campylobacter Coli ◽  
Poultry Products ◽  
Campylobacter Spp ◽  
Southern Benin

Foodborne infections, mainly those attributable to Campylobacter, are one of the most common causes of intestinal diseases, of bacterial origin in humans. Although the vehicle of transmission is not always identified, the most common vehicles are poultry, poultry products, and contaminated water. In Southern Benin, an excessive use of poultry manure as fertilizer in vegetable farms was noted. This survey aimed to determine the prevalence and concentration of Campylobacter spp., especially Campylobacter jejuni and Campylobacter coli, in selected environmental samples (poultry manure, and irrigation water) and freshly harvested leafy vegetables in two (Houeyiho and Sèmè-Kpodji) vegetable farms in southern Benin. To achieve this objective, we analyzed 280 samples, including 224 samples of leafy vegetables (Solanum macrocarpon and Lactuca sativa capita), 28 samples of irrigation water, and 28 samples of poultry manure. The analysis of the samples taken was carried out according to the modified NF EN ISO 10272-1 standard. Of the 280 samples analyzed in this survey, 63 were positive for Campylobacter contamination. For leafy vegetable samples analyzed in this survey, the contamination rate was of 15.63%. 60.71% of poultry manure samples analyzed were contaminated with Campylobacter spp. and 39.29% of irrigation water samples were contaminated. The statistical analysis of these results showed that there is a correlation between the contamination of leafy vegetables, poultry manure, and irrigations (p<0.01). Campylobacter jejuni (53.97%) was more involved in contaminations than Campylobacter coli (36.57%). This study has shown that there is a real risk of food poisoning by Campylobacter jejuni and Campylobacter coli among consumers of leafy vegetables in southern Benin. The origin of contamination of these leafy vegetables is poultry manure used as fertilizer in vegetable gardens and irrigation water used.

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