Accuracy investigation of Fused Deposition Modelling (FDM) processed ABS and ULTRAT parts.

This paper aims to assess the dimensional deviation of Fused Deposition Modeling (FDM) processed ABS and ULTRAT parts using a new geometrical model which can evaluate three types of dimensional deviation: along the z-axis, along external and internal dimensions, and through diameters. The methodology involves a step-by-step procedure wherein after establishing the experimental plan and manufacturing the specimens, the measurements taken are analyzed via Grey Relational Analysis (GRA) to find out the optimal combination of parameters leading to the minimum deviation in all dimensions of parts for both materials. Statistical techniques such as Analysis of Variance (ANOVA) and Signal to Noise (S/N) ratio were also used. Subsequently, a confirmation test was carried out to validate the results obtained. The findings of the ANOVA and the S/N ratio were in good concordance with those of GRA.

Design of experiment analysis of elevated temperature wear of Mg-WC nano-composites

Current study explores the effect of selected process parameters i.e. wt.% of reinforcement (A), elevated temperature (B) and load (C) on wear characteristics of Mg-WC nanocomposites using Taguchi robust design concept. Ultrasonic treated stir casting is employed to synthesize nanocomposites. Three levels for every factor are taken into consideration and accordingly L27 orthogonal array (OA) is used for minimization of wear rate. Main effect plot is generated to investigate the important parameters and optimality is also predicted from the main effect plot. Optimal condition for minimum wear rate is 2wt.% of WC, 100°C temperature and 20N load (A3B1C1). Interaction plots are generated to scrutinize the interaction outcome between selected parameters. ANOVA study is executed to evaluate significant parameters and their effective handout on output. Current investigation reveals, Wt.% of WC is the most significant factor while temperature and load are moderately significant. Among the interacting parameters, interaction between wt.% of WC & temperature (A×B) has moderate significance. Wt.% of WC (A) has 43.135% contribution while temperature (B), load (C) and interaction between wt.% of WC & temperature (A×B) have 26.623%, 19.037% and 5.639% contribution respectively. Residual plots for wear rate are discussed and confirmation test finally helps to validate present experimental model. S/N ratio is improved by 4.411 dB (48.60%) than the initial condition.

Development and validation of an algorithm to estimate the risk of severe complications of COVID-19: a retrospective cohort study in primary care in the Netherlands

ObjectiveTo develop an algorithm (sCOVID) to predict the risk of severe complications of COVID-19 in a community-dwelling population to optimise vaccination scenarios.DesignPopulation-based cohort study.Setting264 Dutch general practices contributing to the NL-COVID database.Participants6074 people aged 0–99 diagnosed with COVID-19.Main outcomesSevere complications (hospitalisation, institutionalisation, death). The algorithm was developed from a training data set comprising 70% of the patients and validated in the remaining 30%. Potential predictor variables included age, sex, chronic comorbidity score (CCS) based on risk factors for COVID-19 complications, obesity, neighbourhood deprivation score (NDS), first or second COVID-19 wave and confirmation test. Six population vaccination scenarios were explored: (1) random (naive), (2) random for persons above 60 years (60plus), (3) oldest patients first in age band of 5 years (oldest first), (4) target population of the annual influenza vaccination programme (influenza), (5) those 25–65 years of age first (worker), and (6) risk based using the prediction algorithm (sCOVID).ResultsSevere complications were reported in 243 (4.8%) people with 59 (20.3%) nursing home admissions, 181 (62.2%) hospitalisations and 51 (17.5%) deaths. The algorithm included age, sex, CCS, NDS, wave and confirmation test (c-statistic=0.91, 95% CI 0.88 to 0.94) in the validation set. Applied to different vaccination scenarios, the proportion of people needed to be vaccinated to reach a 50% reduction of severe complications was 67.5%, 50.0%, 26.1%, 16.0%, 10.0% and 8.4% for the worker, naive, influenza, 60plus, oldest first and sCOVID scenarios, respectively.ConclusionThe sCOVID algorithm performed well to predict the risk of severe complications of COVID-19 in the first and second waves of COVID-19 infections in this Dutch population. The regression estimates can and need to be adjusted for future predictions. The algorithm can be applied to identify persons with highest risks from data in the electronic health records of general practitioners (GPs).

Use of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples

The use of direct PCR technique without DNA extraction in the confirmation test for Salmonella typhimurium ATCC 14028 bacteria on meatball samples was carried out in the Food and Drug molecular biology testing laboratory Administration in Gorontalo. The basis of this research is to have an impact on economic value in carrying out the confirmation test for S. typhimurium ATCC 14028, where testing is carried out conventionally, namely DNA extraction, which requires a large amount of money. Hence, it is necessary to innovate to modify the testing phase so that it is more effective and efficient. The purpose of this study was to see whether the direct PCR technique without DNA extraction can be done for the confirmation test of S. typhimurium ATCC 14028 on meatball samples. This study's sample consisted of 20 types of meatball samples spiked with S. typhimurium ATCC 14028 cultures. The method used in this study was qPCR analysis using the SYBR Green method. Data analysis was carried out based on 2 main criteria: (1) Ct analysis and (2) Tm analysis. Real-time PCR analysis results obtained Ct values ​​in the range 14.14 - 15.20 with an average of 14.82 and Tm values ​​85.20 - 86.30 with an average of 85.79. Based on these data, it can be concluded that using direct PCR can be used for testing confirmation of S. typhimurium ATCC 14028 on meatball samples.

Autonous Aldosterone Secretion as a Subclinical Form of Primary Aldosteronism: Pathogenesis and Clinical Significance

In recent years, a substantial prevalence of primary aldosteronism (PA) has been demonstrated in both normotensive and mildly hypertensive cohorts. Consequently, a classic presentation of the syndrome, i. e. moderate-to-severe and resistant hypertension with concomitant hypokalemia, should be considered a tip-of-the-iceberg phenotype of a wide PA spectrum. Its entire range encompasses the non-classic clinical forms of mild hypertension and prehypertension but also several biochemical presentations, including patients who meet PA screening and confirmation test criteria, as well as those with either of them and those with other parameters indicating mineralocorticoid excess. In the current review, research insights on the pathogenetic background and clinical significance of autonomous aldosterone secretion (AAS) are presented, which is defined as a constellation of either: 1) normotension, normokalemia, a positive PA screening (high aldosterone-to-renin ratio) and/or confirmation test, or 2) hypertension, normokalemia and a positive PA screening but negative confirmation test. For this purpose, a literature search of the PubMed database was conducted. Advances in immunohistochemistry and genetic sequencing of isolated adrenal cells are provided as probable morphologic basis of the wide range of aldosterone secretion autonomy. Also, the role of corticotropin as an aldosterone secretagogue is discussed. To date, clinical studies depict consequences of subclinical PA phenotypes, such as increased mortality and risk of developing hypertension, impaired arterial and kidney function, association with metabolic syndrome and age, as well as osteoporosis.

Explosion Shock Measurement System of the Precursor Warhead for the Tandem Projectile

This paper presents a system that measures the acceleration of the shock caused by the explosion of the precursor warhead for the tandem projectile. The proposed system, which is implemented based on the MIL-STD-810G, Method 517.1, consists of a miniaturized shock measurement device, a cable, accelerometers, and a trigger circuit. The shock measurement device has a size of ￠102 x 171 mm and cable has a length of 3 m. The operational confirmation test is conducted by implementing the measurement system. The Analysis of shock data(accelerometer output data) is carried out using Shock Response Spectrum(SRS), pseudo velocity and plot of acceleration time transient. Through measurement analysis, one can predict the damage of electronics in projectile when precursor warhead is exploded.

Miastenia Gravis Okular Juvenil: Laporan Kasus

Purpose: This study aimed to summarize the general history, physical exam findings, confirmation test, and treatment of a patient with juvenile ocular myasthenia gravis. Research Methodology: This article is a pediatric RSUD Abdoel Moeloek Lampung case report in April 2021. The subject of the case is a 3-year-old girl who is hospitalized in the hospital ward with juvenile ocular myasthenia gravis. Result: A 3-year-old girl, an inpatient in the Pediatric Ward, RSUD Abdoel Moeloek Lampung, with complaints of decreased left upper eyelid since three months ago. On physical examination, there was ptosis of the left superior palpebra. Prostigmine test was performed on day 2 of treatment in patients with positive test results. The patient was clinically diagnosed with “Juvenile Ocular Myasthenia Gravis.” Management of the patient during treatment was the injection of methylprednisolone 10 mg/12 hours and neostigmine orally 2.5 mg/8 hours. Limitations: This case report is limited to juvenile ocular myasthenia gravis cases with a scarce number of cases and rare cases in children. Contribution: This case report can be helpful in medical education or used as a reference in making relevant case reports and can be applied in the hospital area when there are cases of juvenile ocular myasthenia gravis.

Pemeriksaan Bercak Darah pada Kain yang Direndam & Dikubur Menggunakan Tes Teichmann

Background. Blood is the most important physical evidences and often found at crime scene. The Teichmann test is a confirmation test to check whether the spot really a blood.Objective. This study aims to identify the bloodstain on cloth soaked in water and buried in the ground using Teichmann test.Method. The research type is a descriptive study with a laboratory experimental study design. The sample of this study was a cloth dripped with blood, 27 samples are immersed in a bucket filled with water and 27 other samples are buried in the ground with a depth of 20 cm. The examination using the Teichmann test will be carried out on the 6th to the 14th day of exposure.Result. From the research that has been done, the results of the Teichmann test were positive on blood spots on cloth soaked in water and buried in the ground on the 6th to 9th day of exposure. Positive results indicate the formation of hemin hydrochloride crystals in the form of blackish-brown rods.Conclusion. The conclusion of this study is hemin hydrochloride crystals can still be found in blood stains on cloth soaked in water and buried in the soil using the Teichmann test but limited to the 9th day of exposure.

Confirmation Value of Western Blotting in Detecting Anti-treponema Pallidum Specific Antibodies With Suspicious Results

ObjectiveThis study aimed to evaluate the necessity of Western blotting (WB) in samples with inconsistent results in detecting anti-treponema pallidum (TP) antibodies by enzyme-linked immunosorbent assay (ELISA) and Treponema pallidum granule agglutination assay (TPPA). MethodsSpecific anti-TP test results in our clinical laboratory were retrospectively analyzed. The specimens with a positive or a negative result, but with colored ELISA plates, were retested by TPPA. WB was used to confirm the suspicious results between ELISA and TPPA. The chi-square test was used to analyze whether the difference was statistically significant. ResultsA total of 106,757 anti-TP specimens were screened by ELISA from August 2018 to December 2019; 3972 were retested by TPPA, and 3809 were positive by TPPA. ELISA and TPPA showed different results in 163 specimens. Among them, 29 specimens were negative and 134 were positive by ELISA; 76 were negative, 23 were positive, and 64 were “reserve” by TPPA; 93 were negative, 31 were positive, and 39 were suspicious by the WB confirmation test. Compared with WB, the difference in the results of ELISA and TPPA was statistically significant. ConclusionsTPPA is an effective retest method for anti-TP antibody detection. If the results of anti-TP antibodies by ELISA and TPPA are inconsistent, it is necessary to use WB for confirmation.

Optimizing Natural Deep Eutectic Solvent Citric Acid-Glucose Based Microwave-Assisted Extraction of Total Polyphenols Content from Eleutherine bulbosa (Mill.) Bulb

Application of natural deep eutectic solvent (NADES) citric acid-glucose-based microwave-assisted extraction (MAE) method for total polyphenol content (TPC) enriches the use of Eleutherine bulbosa (Mill.) bulb more widely and commercially. Therefore, this study aims to optimize NADES citric acid-glucose-based MAE on the TPC from E. bulbosa bulbs using response surface methodology (RSM). In the present study, the dried sample of E. bulbosa bulb was extracted using the NADES based MAE. Determination of TPC was conducted using Folin-Ciocalteau reagent and standard gallic acid, then measured using a UV-Vis spectrophotometer at 761 nm. Extraction condition optimization of the NADES based MAE method was performed using RSM with Box Behnken Design (four-factors-three-levels and 29 runs) on Design Expert v12. Based on the results study, the optimum condition extraction on the TPC enrichment was obtained at the NADES ratio (citric acid: glucose) 1:1 g/g; solid-liquid ratio 1:8 g/mL; extraction time of 15 min; and 270 Watt microwave power. The confirmation test and scale-up (50 g samples) were obtained with a 61.63 ± 2.23 mg GA/g sample.