scholarly journals Lower quality bovine embryos may be successfully used for sex determination

2008 ◽  
Vol 52 (No. 12) ◽  
pp. 540-546
Author(s):  
M. Lopatarova ◽  
S. Cech ◽  
P. Krontorad ◽  
L. Holy ◽  
J. Hlavicova ◽  
...  
Keyword(s):  
Sex Determination ◽  
Pcr Analysis ◽  
Embryonic Cells ◽  
Perivitelline Space ◽  
Bovine Embryos ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Pcr Method ◽  
Polymerase Chain ◽  
And Gender

The aim of this study was to evaluate the effect of sex determination procedures in Day 7 to Day 8 bovine embryos of various quality. For the purposes of comparison we used high quality (HQ) as well as lower quality (LQ) embryos obtained from superovulated donors. The healthy embryonic cells isolated from HQ embryos and blastomeres protruding into the perivitelline space of LQ embryos, were analysed by polymerase chain reactions (PCR) using primers specific for the Y chromosome determinant. After microsurgical intervention and completion of sex determination, the female embryos were then transferred (ET) to synchronized recipients. A total of 310 embryos of HQ were used and gender was safely determined in 275 cases (88.7%). PCR analysis of extruded cells isolated from 170 LQ embryos was carried out with certainty only in 111 embryos (65.3%, <I>P</I> < 0.01). After ET of 122 HQ sex defined embryos, pregnancy was established in 69 recipients (56.6%). A similar conception rate 51.9% (27/52) was found after the ET of sex defined embryos designated as LQ. The accuracy of analysis was confirmed after calving and revealed that designated female sex coincided with 95.5% and 96.2% of calves when HQ and LQ embryos were transferred, respectively. Our results clearly show that a microsurgical technique in combination with PCR method represents a rapid and reliable approach for sex determination in HQ as well as in LQ preimplantation bovine embryos and can be used in field conditions for the regulation of the sex of progeny in selected herds.

scholarly journals Sex determination in gibbons of genus Nomascus using non-invasive method

2016 ◽  
Vol 85 (4) ◽  
pp. 363-366 ◽  
Author(s):  
Petra Bolechová ◽  
Kateřina Ječmínková ◽  
Michal Hradec ◽  
Tomáš Kott ◽  
Jana Doležalová
Keyword(s):  
Sex Determination ◽  
Direct Examination ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Non Invasive ◽  
Faecal Samples ◽  
Invasive Method ◽  
Polymerase Chain ◽  
Template Dna

Gibbons of the genus Nomascus have a strong sexual dimorphism and dichromatism. As they mature, both sexes develop sex-specific pelage colour. In combination with physical similarities in the genitalia with both sexes, there are problems with determining the sex of young individuals compared to other genus of gibbons. This is a pilot study applying a multiplex polymerase chain reactions based on a non-invasive method for sex determination of gibbons. The study was conducted on 22 faecal samples from gibbons of the genus Nomascus. The animals were monitored by staff so that the samples were identified correctly and each sample was collected immediately after the defecation. Results confirmed the sex in all adult and juvenile animals with known sex; and 2 females and 5 males in juveniles were determined with unknown sex. The results of direct examination completely corresponded with the PCR results. The PCR reaction with template DNA isolated from faecal material required BSA usage, however, we observed the occurrence of nonspecific fragments. This did not affect the reliability of our results and we confirmed the usability of this method for this genus.

scholarly journals Investigation of Lethal Concurrent Outbreak of Chlamydiosis and Pigeon Circovirus in a Zoo

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1654
Author(s):  
Wei-Tao Chen ◽  
Chin-Ann Teng ◽  
Cheng-Hsin Shih ◽  
Wei-Hsiang Huang ◽  
Yi-Fan Jiang ◽  
...  
Keyword(s):  
Disease Outbreaks ◽  
Chlamydia Psittaci ◽  
Bursa Of Fabricius ◽  
Chain Reactions ◽  
Renal Epithelium ◽  
Polymerase Chain Reactions ◽  
Transmission Electron ◽  
Intracytoplasmic Inclusion Bodies ◽  
Polymerase Chain ◽  
Streptopelia Orientalis

During the spring, an outbreak of sudden death involving 58 birds occurred in a zoo. Histopathological examinations revealed variable numbers of intracytoplasmic basophilic microorganisms in the macrophages, hepatocytes, and renal epithelium of most birds, along with occasional botryoid intracytoplasmic inclusion bodies within histiocytes in the bursa of Fabricius. Based on the results of histopathological examinations, immunohistochemical staining, transmission electron microscopy, and polymerase chain reactions, genotype B Chlamydia psittaci infection concurrent with pigeon circovirus (PiCV) was diagnosed. A retrospective survey, including two years before the outbreak and the outbreak year, of C. psittaci and PiCV infections of dead birds in the aviaries, revealed that the outbreak was an independent episode. The findings of this study indicate that concurrent infection with C. psittaci and PiCV might lead to lethal outbreaks of chlamydiosis, particularly Streptopelia orientalis. In addition, persistently monitoring both pathogens and identifying potential PiCV carriers or transmitters might also help prevent lethal disease outbreaks.

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