Extraction conditions evaluation of pectin methylesterase produced by solid state culture of Aspergillus niger
2019 ◽
Vol 36
(No. 6)
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pp. 476-479
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Tween 80
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Different solvents and extraction solutions (distilled water, NaCl, Tween 80, citrate buffer, and acetate buffer) were evaluated for enzymes recovery. The independent variables evaluated were agitation (12–348 rpm), time (4.8–55.2 min), and temperature (13.2–46.8°C) and 10 extraction cycles, using an experimental design (Central composite rotatable design 2<sup>3</sup>). Pectin methyl esterase maximum recovery by solid state culture of Aspergillus niger was 11 U/g<sub>wm</sub> (31 U/g<sub>dm</sub>) using NaCl (0.1 mol/l) as solvent at a 5 : 1 (v/w) ratio, 30°C for 55.2 min, at 180 rpm, with one extraction cycle.
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2012 ◽
Vol 47
(12)
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pp. 2199-2203
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1999 ◽
Vol 34
(2)
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pp. 181-186
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1998 ◽
Vol 23
(1-2)
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pp. 149-156
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2008 ◽
Vol 2
(2)
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pp. 208-212
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2015 ◽
Vol 176
(4)
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pp. 1131-1140
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1990 ◽
Vol 12
(7)
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pp. 546-551
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