scholarly journals Cryoprotection of porcine cornea: a scanning electron microscopy study 

2012 ◽  
Vol 50 (No. 5) ◽  
pp. 219-224 ◽  
Author(s):  
A. Neronov ◽  
P. Giurov ◽  
M. Cholakova ◽  
M. Dimitrova ◽  
E. Nikolova
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Blue Staining ◽  
Peg 400 ◽  
Temperature Regimen ◽  
Protective Medium ◽  
Scanning Electron ◽  
Porcine Cornea

Porcine corneas were frozen with Me<sub>2</sub>SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1&ordm;C/min and 5&ordm;C/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan blue staining. The presence of 5&ndash;10% PEG-400 in the protective medium was the most effective in minimizing changes in the integrity of the corneal endothelium during freezing-thawing procedures.

To morphology, ecology and distribution of rare species Sellaphora hustedtii (Krasske) Lange-Bertalot et Werum (Bacillariophyta) from waterbodies of the Gydan Peninsula

2014 ◽  
Vol 48 ◽  
pp. 57-65 ◽  
Author(s):  
S. I. Genkal ◽  
M. I. Jarushina
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
River Basin ◽  
Rare Species ◽  
Western Siberia ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Diagnostic Features ◽  
Scanning Electron Microscopy Study ◽  
Scanning Electron

A rare species Sellaphora hustedtii, which is also new for the flora of Russia, has been found in waterbodies of the Messoyakha River basin (south-western part of the Gydan Peninsula, Western Siberia). The scanning electron microscopy study revealed variability of the main quantitative and qualitative diagnostic features of the species (length and width of the valve, number of striae and areolae in 10 μm, shape of the valve and structure of the raphe). New data on the ecology and distribution of the species allowed us to specify its diagnosis.

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