scholarly journals Content of phenolic acids in rye caryopses determined using DAD-HPLC method

2013 ◽  
Vol 19 (No. 6) ◽  
pp. 201-205 ◽  
Author(s):  
R. Amarowicz ◽  
S. Weidner
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Hplc Method ◽  
Uv Spectra ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Uv Spectrum ◽  
Free Phenolic Acids

Phenolic compounds were extracted from rye caryopses with 80% (v/v) methanol. Phenolic acids were determined as free compounds and those liberated from soluble esters and glycosides. The analyses were performed using a Waters HPLC system equipped with a diode array detector (DAD). The following free phenolic acids were found: p-coumaric, ferulic and sinapic; the phenolic acids liberated from soluble esters were as follows: vanillic, caffeic, p-coumaric, ferulic and sinapic; and those liberated from soluble glycosides were the following: vanillic, p-coumaric, ferulic and sinapic. In rye caryopses, phenolic acids were chiefly in the form of soluble esters. A diode array detector was especially useful for the determination of vanillic acid: the UV spectrum of this compound showed a maximum at 260 nm whereas UV spectra of other phenolic acids were characterised by maxima at longer wavelengths.

scholarly journals Quantitative Determination of Chemical Constituents from Seeds of Nigella sativa L. Using HPLC-UV and Identification by LC-ESI-TOF

2010 ◽  
Vol 93 (6) ◽  
pp. 1778-1787 ◽  
Author(s):  
Bharathi Avula ◽  
Yan-Hong Wang ◽  
Zulfiqar Ali ◽  
Ikhlas A Khan
Keyword(s):  
Nigella Sativa ◽  
Chemical Constituents ◽  
Hplc Method ◽  
Array Detector ◽  
Diode Array ◽  
Gradient System ◽  
Diode Array Detector ◽  
Column Material ◽  
Positive Ion

Abstract An HPLC method was developed for the simultaneous determination of nine compounds of Nigella sativa L. The separation was achieved within 23 min by using C18 column material, a wateracetonitrile mobile phase, both containing 0.1 acetic acid gradient system and a temperature of 35C. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ of nine compounds were in the range of 0.0910 and 0.325 g/mL, respectively. The wavelength used for quantification with the diode array detector was 205 and 260 nm. LC/MS coupled with electrospray ionization interface method is described for the identification of compounds in N. sativa L. samples. This method involved the use of [MH]<sup/> and [MNa]<sup/> ions in the positive ion mode with extracted ion chromatogram.

Determination of phenolic acids and a flavonoid in Eleusine coracana (L.) by semi-preparative HPLC photo diode array detector

2015 ◽  
Vol 70 (3) ◽  
pp. 369-373
Author(s):  
Jignasu P. Mehta ◽  
Chirag R. Fultariya ◽  
Pravin H. Parmar ◽  
Sohil H. Vadia ◽  
Balubhai A. Golakiya
Keyword(s):  
Phenolic Acids ◽  
Eleusine Coracana ◽  
Array Detector ◽  
Diode Array ◽  
Preparative Hplc ◽  
Diode Array Detector ◽  
Photo Diode

scholarly journals Quantititative Determination of Lycorine and Galanthamine in Galanthus trojanus and G. cilicicus by HPLC-DAD

2014 ◽  
Vol 9 (8) ◽  
pp. 1934578X1400900
Author(s):  
Gulen Irem Kaya ◽  
Derya Cicek Polat ◽  
Buket Sarikaya ◽  
Mustafa Ali Onur ◽  
Nehir Unver Somer
Keyword(s):  
Biological Activities ◽  
Hplc Method ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Simple Method ◽  
Aerial Parts ◽  
Flowering Season ◽  
Low Mass

Lycorine and galanthamine have various biological activities. A reliable HPLC method coupled with DAD detection was developed and validated for the determination of galanthamine and lycorine in Galanthus trojanus and G. cilicicus. A simple method for the extraction of the alkaloids in low-mass plant samples was employed utilizing columns pre-packed with diatomaceous earth (Extrelut®). This method was applied to the aerial parts and bulbs of G. trojanus and G. cilicicus (Amaryllidaceae) collected during the flowering season. The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5) applied at a flow rate of 1 mL min−1 and using a diode array detector. Validation procedures showed that the method was specific, accurate and precise. The highest amount of lycorine (0.012%) was detected in the bulbs of G. trojanus collected from Çan (Çanakkale), whereas the aerial parts of this species collected from Bayramiç (Çanakkale) was not found to contain this alkaloid. In G. cilicicus samples, lycorine was only determined in the bulbs, giving yields of 0.004%; galanthamine yields were between 0.015-0.016%, but none of the G. trojanus samples contained this latter alkaloid.

scholarly journals Quantitative Determination by HPLC-DAD of Icariin, Epimedin A, Epimedin B, and Epimedin C in Epimedium (Berberidaceae) Species Growing in Turkey

2016 ◽  
Vol 11 (11) ◽  
pp. 1934578X1601101 ◽  
Author(s):  
Derya Cicek Polat ◽  
Maksut Coskun
Keyword(s):  
Quantitative Determination ◽  
Flow Rate ◽  
Mobile Phase ◽  
Hplc Method ◽  
Biologically Active ◽  
Array Detector ◽  
Diode Array ◽  
Gradient System ◽  
Diode Array Detector

The genus Epimedium is rich in terms of flavonoids, of which icariin, epimedin A, epimedin B and epimedin C are known especially to be biologically active. Therefore, it is important to quantify these compounds. In this study, a HPLC method coupled with DAD detection was developed and validated for the determination of icariin, epimedin A, epimedin B and epimedin C in Epimedium species growing in Turkey. The chromatographic separation was performed using a gradient system with a mobile phase of 0.1% formic acid (A) and acetonitrile (B) applied at a flow rate of 1 mL/min using a diode array detector. The highest values were, respectively, icariin 0.65%, epimedin A 0.13%, epimedin B 0.11%, epimedin C 0.06%. The highest values were obtained from the materials collected in Uzungol (Trabzon-Turkey).

scholarly journals Simultaneous RP-HPLC determination of sparfloxacin and dexamethasone in pharmaceutical formulations

2013 ◽  
Vol 49 (2) ◽  
pp. 301-309 ◽  
Author(s):  
Syed Naeem Razzaq ◽  
Muhammad Ashfaq ◽  
Irfana Mariam ◽  
Islam Ullah Khan ◽  
Syed Saleem Razzaq
Keyword(s):  
Degradation Products ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Array Detector ◽  
Diode Array ◽  
Stability Studies ◽  
Diode Array Detector ◽  
Rp Hplc ◽  
Peak Purity

The present study describes the development and subsequent validation of simple and accurate stability indicating RP-HPLC method for the determination of sparfloxacin and dexamethasone in pharmaceutical formulations in the presence of their stress-induced degradation products. Both the drugs and their stress-induced degradation products were separated within 10 minutes using C8 column and mixture of methanol and 0.02 M phosphate buffer pH 3.0 (60:40 v/v, respectively) as mobile phase at 270 nm using diode array detector. Regression analysis showed linearity in the range of 15-105 µg/mL for sparfloxacin and 5-35 µg/mL for dexamethasone. All the analytes were adequately resolved with acceptable tailing. Peak purity of the two drugs was also greater than 0.9999, showing no co-elution peaks. The developed method was applied for simultaneous determination of sparfloxacin and dexamethasone in pharmaceutical formulations for stability studies.

scholarly journals Analysis of the Mycosporine-Like Amino Acid (MAA) Pattern of the Salt Marsh Red Alga Bostrychia scorpioides

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 321
Author(s):  
Maria Orfanoudaki ◽  
Anja Hartmann ◽  
Julia Mayr ◽  
Félix L. Figueroa ◽  
Julia Vega ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Red Alga ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
The Past ◽  
Specific Species

This study presents the validation of a high-performance liquid chromatography diode array detector (HPLC-DAD) method for the determination of different mycosporine-like amino acids (MAAs) in the red alga Bostrychia scorpioides. The investigated MAAs, named bostrychines, have only been found in this specific species so far. The developed HPLC-DAD method was successfully applied for the quantification of the major MAAs in Bostrychia scorpioides extracts, collected from four different countries in Europe showing only minor differences between the investigated samples. In the past, several Bostrychia spp. have been reported to include cryptic species, and in some cases such as B. calliptera, B. simpliciuscula, and B. moritziana, the polyphyly was supported by differences in their MAA composition. The uniformity in the MAA composition of the investigated B. scorpioides samples is in agreement with the reported monophyly of this Bostrychia sp.

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